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The map label for this gene is scrR [H]

Identifier: 126209486

GI number: 126209486

Start: 2258469

End: 2259488

Strand: Reverse

Name: scrR [H]

Synonym: APL_2032

Alternate gene names: 126209486

Gene position: 2259488-2258469 (Counterclockwise)

Preceding gene: 126209487

Following gene: 126209485

Centisome position: 99.34

GC content: 43.43

Gene sequence:

>1020_bases
GTGGAATCCAAAGCTAAAAAACGCCTGACACTTAGCGATATAGCGGCACTTAGCGGCGTGTCGAAAACGACGGCAAGTAT
GATTCTCAACGGTAGAAGCGATGATTTCCGCATCAAACTTGAAACGCGCCAAAAAGTTCAAGCAATCGCCGAACAATACG
GTTATCGTGCGAATATCTATGCGAAAGCGTTACAAGCGCAGCGTTCTAACGTAATCGGTTTGGTAATTCCCGACTTGACC
AACTACGGCTTTGCTTCCACCGCCCGTAATCTTGAAAAACTGTGCCGAGAAAACGGTTTACAGCTTGTGATCGCTTGTTC
CGACGATAATCCTCAACAAGAAAAACTGGTGATTGATCGTTTGCTTGATCGCCAAGTGGATTTACTGATTACCGCTCCGA
CACATCAAGACCCGAATTATTATAAAAAAATCATTAGGCATACGCCGGTGTTACATATCGACCGCCATATTCCGAATTTG
GAGCTGAATTACATTATCAGCGACGACACGCCCAGCGTAGCGAAATTGGTGGAAAATATCGTAAGAGCCTATCAACCGAA
AGAATTTTTCTATTTAGGCGGACAACTTTCGCTTTCGCCGAGTGCTTCTCGTTTGGAAGGCTTTTACGAAGGCTTGGAGC
AATCGCATTTAGCGGTAAAAAGCGATTGGATTTTACATAAAGATTACCAACCGGAATCGGGTTATCAAATGTTCGCCGAA
GTGGTTGAACGCTTAGGACGCTTACCCGAAGCGGTGTTTACCGCCTCTTATACCATTTTGGAAGGCGTGTTGCGTTATCT
GACCGAACATAAACAAATGGCGAAATTGATGAATCAAGAATTACATTTAGCTACGTTTGACGATCATCATTTACTTGACG
CACTCCCCTTTCATATTCACTCGATTGCGCAAGACCACGATCAAATTGCATTAAGAACTTTCCAATTAATGCGAGAAAAA
CTGCAGCGTAAAACGATTAACAATGCCAAAGTAGATTGCGAAATTATTTGGCGACACTAA

Upstream 100 bases:

>100_bases
GTGCAGCCGATTCAAATTCAAGATAAATAATAACAATAGGCGCAAGCAAAACTTGCGCCCTTTCGCTTCAAAATTAGAAG
CCACCTTTGAGGAGAACATT

Downstream 100 bases:

>100_bases
AACCGATACCGTTAATTTTAAAAGGATAACCTATGGCACATTTTTATGAATATTTAGAATTTAGCAGTGACGAAGACCGT
GAAAATCAGCTTGAAGTTTA

Product: sucrose operon repressor (Scr operon regulatory protein)

Products: NA

Alternate protein names: Scr operon regulatory protein [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MESKAKKRLTLSDIAALSGVSKTTASMILNGRSDDFRIKLETRQKVQAIAEQYGYRANIYAKALQAQRSNVIGLVIPDLT
NYGFASTARNLEKLCRENGLQLVIACSDDNPQQEKLVIDRLLDRQVDLLITAPTHQDPNYYKKIIRHTPVLHIDRHIPNL
ELNYIISDDTPSVAKLVENIVRAYQPKEFFYLGGQLSLSPSASRLEGFYEGLEQSHLAVKSDWILHKDYQPESGYQMFAE
VVERLGRLPEAVFTASYTILEGVLRYLTEHKQMAKLMNQELHLATFDDHHLLDALPFHIHSIAQDHDQIALRTFQLMREK
LQRKTINNAKVDCEIIWRH

Sequences:

>Translated_339_residues
MESKAKKRLTLSDIAALSGVSKTTASMILNGRSDDFRIKLETRQKVQAIAEQYGYRANIYAKALQAQRSNVIGLVIPDLT
NYGFASTARNLEKLCRENGLQLVIACSDDNPQQEKLVIDRLLDRQVDLLITAPTHQDPNYYKKIIRHTPVLHIDRHIPNL
ELNYIISDDTPSVAKLVENIVRAYQPKEFFYLGGQLSLSPSASRLEGFYEGLEQSHLAVKSDWILHKDYQPESGYQMFAE
VVERLGRLPEAVFTASYTILEGVLRYLTEHKQMAKLMNQELHLATFDDHHLLDALPFHIHSIAQDHDQIALRTFQLMREK
LQRKTINNAKVDCEIIWRH
>Mature_339_residues
MESKAKKRLTLSDIAALSGVSKTTASMILNGRSDDFRIKLETRQKVQAIAEQYGYRANIYAKALQAQRSNVIGLVIPDLT
NYGFASTARNLEKLCRENGLQLVIACSDDNPQQEKLVIDRLLDRQVDLLITAPTHQDPNYYKKIIRHTPVLHIDRHIPNL
ELNYIISDDTPSVAKLVENIVRAYQPKEFFYLGGQLSLSPSASRLEGFYEGLEQSHLAVKSDWILHKDYQPESGYQMFAE
VVERLGRLPEAVFTASYTILEGVLRYLTEHKQMAKLMNQELHLATFDDHHLLDALPFHIHSIAQDHDQIALRTFQLMREK
LQRKTINNAKVDCEIIWRH

Specific function: Repressor for the scr operon. Binds D-fructose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786268, Length=313, Percent_Identity=33.5463258785943, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1787948, Length=317, Percent_Identity=23.0283911671924, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790369, Length=353, Percent_Identity=24.6458923512748, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790194, Length=333, Percent_Identity=22.5225225225225, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789068, Length=283, Percent_Identity=24.0282685512367, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787906, Length=303, Percent_Identity=25.7425742574257, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789202, Length=280, Percent_Identity=25, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI48994940, Length=301, Percent_Identity=25.2491694352159, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788474, Length=283, Percent_Identity=23.321554770318, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012781
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38876; Mature: 38876

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKAKKRLTLSDIAALSGVSKTTASMILNGRSDDFRIKLETRQKVQAIAEQYGYRANIY
CCCCHHHCCHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCHHHH
AKALQAQRSNVIGLVIPDLTNYGFASTARNLEKLCRENGLQLVIACSDDNPQQEKLVIDR
HHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
LLDRQVDLLITAPTHQDPNYYKKIIRHTPVLHIDRHIPNLELNYIISDDTPSVAKLVENI
HHCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHH
VRAYQPKEFFYLGGQLSLSPSASRLEGFYEGLEQSHLAVKSDWILHKDYQPESGYQMFAE
HHHCCCCCEEEECCEEEECCCHHHHHHHHHHHHHHHHEEECCEEEECCCCCCCHHHHHHH
VVERLGRLPEAVFTASYTILEGVLRYLTEHKQMAKLMNQELHLATFDDHHLLDALPFHIH
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHH
SIAQDHDQIALRTFQLMREKLQRKTINNAKVDCEIIWRH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
>Mature Secondary Structure
MESKAKKRLTLSDIAALSGVSKTTASMILNGRSDDFRIKLETRQKVQAIAEQYGYRANIY
CCCCHHHCCHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCHHHH
AKALQAQRSNVIGLVIPDLTNYGFASTARNLEKLCRENGLQLVIACSDDNPQQEKLVIDR
HHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
LLDRQVDLLITAPTHQDPNYYKKIIRHTPVLHIDRHIPNLELNYIISDDTPSVAKLVENI
HHCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHH
VRAYQPKEFFYLGGQLSLSPSASRLEGFYEGLEQSHLAVKSDWILHKDYQPESGYQMFAE
HHHCCCCCEEEECCEEEECCCHHHHHHHHHHHHHHHHEEECCEEEECCCCCCCHHHHHHH
VVERLGRLPEAVFTASYTILEGVLRYLTEHKQMAKLMNQELHLATFDDHHLLDALPFHIH
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHH
SIAQDHDQIALRTFQLMREKLQRKTINNAKVDCEIIWRH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412665 [H]