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The map label for this gene is hisA

Identifier: 126209480

GI number: 126209480

Start: 2253896

End: 2254648

Strand: Direct

Name: hisA

Synonym: APL_2026

Alternate gene names: 126209480

Gene position: 2253896-2254648 (Clockwise)

Preceding gene: 126209479

Following gene: 126209481

Centisome position: 99.09

GC content: 47.01

Gene sequence:

>753_bases
ATGCAAAAAAAATCAATCATCATCCCCGCCCTCGATTTAATCGACGGCAATGTCGTTCGCCTACATCAAGGGGACTATGC
GAAACAAACCACCTATTCCGATAATCCGATTGAGCAATTCGCTAATTATCTCGCACAAGGGGCGGAGCAGTTGCATTTGG
TGGATCTGACGGGGGCGAAATATCCTGCAAAAAGACAGACCGCACTTATTGGAAAGATTATTGCGGAGACAAATTGCCAA
ATTCAAGTGGGCGGTGGTATTCGTACCGAGCAAGACGTGGCGGATTTATTGGCGGTGGGGGCAAATCGTGTAGTGATTGG
CTCAACCGCAGTGAAAGATCGGGCAATGGTCAAAGGCTGGTTTGAAAAATACGGCGCGGAAAAATTCGTGCTGGCGTTGG
ACGTGAACATTGACGCAAGCGGTCAAAAAATCATTGCGATTAGCGGTTGGCAGGAAGCGAGTGGTGTGTCGTTGGAAGAG
CTGATTGAAGATTATCAAGTGGTCGGTTTACAACACGTTTTATGTACCGATATTTCCCGAGATGGCACGTTAGCTGGCTC
AAATGTGAATCTTTACCGTGAAATCTGTGCCAAATATCCTAAAATCCATTTCCAATCATCAGGCGGAATTGGCTCGCTTG
ACGATATTAAGGCGTTAAAAGGCACAGGTGTGTCGGGCGTGATCGTAGGGCGAGCGTTGTTAGAAGGCAAATTTAATGTA
GCGGAGGCAATCGAATGTTGGCAAAACGGATAA

Upstream 100 bases:

>100_bases
GGCTACATAATCCGAGCCACGCTGTGGCTCTTTGATACAGCCGCAGAGCGGCTGAGTTTATTCAGCCTAGCACGGCTCGT
GCCAGGTACAGGAACACAAT

Downstream 100 bases:

>100_bases
TCCCTTGTTTGGACGTGCGTGATGGTCAGGTGGTCAAAGGCGTGCAGTTCCGCAACCACGAAATTATCGGCGACATCGTA
CCGCTTGCGAAACGCTATGC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MQKKSIIIPALDLIDGNVVRLHQGDYAKQTTYSDNPIEQFANYLAQGAEQLHLVDLTGAKYPAKRQTALIGKIIAETNCQ
IQVGGGIRTEQDVADLLAVGANRVVIGSTAVKDRAMVKGWFEKYGAEKFVLALDVNIDASGQKIIAISGWQEASGVSLEE
LIEDYQVVGLQHVLCTDISRDGTLAGSNVNLYREICAKYPKIHFQSSGGIGSLDDIKALKGTGVSGVIVGRALLEGKFNV
AEAIECWQNG

Sequences:

>Translated_250_residues
MQKKSIIIPALDLIDGNVVRLHQGDYAKQTTYSDNPIEQFANYLAQGAEQLHLVDLTGAKYPAKRQTALIGKIIAETNCQ
IQVGGGIRTEQDVADLLAVGANRVVIGSTAVKDRAMVKGWFEKYGAEKFVLALDVNIDASGQKIIAISGWQEASGVSLEE
LIEDYQVVGLQHVLCTDISRDGTLAGSNVNLYREICAKYPKIHFQSSGGIGSLDDIKALKGTGVSGVIVGRALLEGKFNV
AEAIECWQNG
>Mature_250_residues
MQKKSIIIPALDLIDGNVVRLHQGDYAKQTTYSDNPIEQFANYLAQGAEQLHLVDLTGAKYPAKRQTALIGKIIAETNCQ
IQVGGGIRTEQDVADLLAVGANRVVIGSTAVKDRAMVKGWFEKYGAEKFVLALDVNIDASGQKIIAISGWQEASGVSLEE
LIEDYQVVGLQHVLCTDISRDGTLAGSNVNLYREICAKYPKIHFQSSGGIGSLDDIKALKGTGVSGVIVGRALLEGKFNV
AEAIECWQNG

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=69.2622950819672, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1788336, Length=245, Percent_Identity=22.4489795918367, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_ACTP2 (A3N3W4)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054705.1
- ProteinModelPortal:   A3N3W4
- STRING:   A3N3W4
- GeneID:   4850124
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_2026
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- PhylomeDB:   A3N3W4
- ProtClustDB:   PRK00748
- BioCyc:   APLE416269:APL_2026-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26890; Mature: 26890

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKSIIIPALDLIDGNVVRLHQGDYAKQTTYSDNPIEQFANYLAQGAEQLHLVDLTGAK
CCCCEEEEEEHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCC
YPAKRQTALIGKIIAETNCQIQVGGGIRTEQDVADLLAVGANRVVIGSTAVKDRAMVKGW
CCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHH
FEKYGAEKFVLALDVNIDASGQKIIAISGWQEASGVSLEELIEDYQVVGLQHVLCTDISR
HHHCCCCEEEEEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
DGTLAGSNVNLYREICAKYPKIHFQSSGGIGSLDDIKALKGTGVSGVIVGRALLEGKFNV
CCEEECCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCH
AEAIECWQNG
HHHHHHHCCC
>Mature Secondary Structure
MQKKSIIIPALDLIDGNVVRLHQGDYAKQTTYSDNPIEQFANYLAQGAEQLHLVDLTGAK
CCCCEEEEEEHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCC
YPAKRQTALIGKIIAETNCQIQVGGGIRTEQDVADLLAVGANRVVIGSTAVKDRAMVKGW
CCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHH
FEKYGAEKFVLALDVNIDASGQKIIAISGWQEASGVSLEELIEDYQVVGLQHVLCTDISR
HHHCCCCEEEEEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
DGTLAGSNVNLYREICAKYPKIHFQSSGGIGSLDDIKALKGTGVSGVIVGRALLEGKFNV
CCEEECCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCH
AEAIECWQNG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA