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The map label for this gene is hisH [H]

Identifier: 126209479

GI number: 126209479

Start: 2253185

End: 2253775

Strand: Direct

Name: hisH [H]

Synonym: APL_2025

Alternate gene names: 126209479

Gene position: 2253185-2253775 (Clockwise)

Preceding gene: 126209478

Following gene: 126209480

Centisome position: 99.06

GC content: 46.36

Gene sequence:

>591_bases
ATGAATTTAGTCATCATCAACACAGGTTGTGCAAACCTGTCATCTGTAAAATTTGCATTCGATCGCTTGGGTATTCAGGC
GGAGATTAGTTGTGATTTAGACAAAATCAAATCAGCGGATAAATTGCTGTTGCCCGGAGTGGGGACGGCTGCGGCGGCGA
TGAAGATTTTGCAAGATCGCGATTTGATTGACACGATCCGCAACGCCACTCAGCCAATGCTTGGTATTTGTTTGGGTATG
CAGTTAATGACTGAATTTTCTGCCGAAGGCGATGTGGACACACTCAAGCTAATGAGTGGCAAAACCGAGTTAATCCCCAA
TACAGGTTTGCCGTTGCCACATATGGGCTGGAACAAGGTTCAGTATCAAGCCGACCACCCGTTATTTCACGGCATTGAGC
AAAACAGCCATTTCTATTTTGTACATAGCTATGCGGTGTTGCCGAATGAAAACACCATTGCTACCGCTGATTATGGCGTG
CCTTTCTCGGCTGCGATTGCGAGCAAAAACTTCTACGGCGTACAGTTCCACCCCGAGCGTTCGGGCAAAAATGGCGCACA
GTTGTTGCGAAATTTTGTGGAGAATATTTAA

Upstream 100 bases:

>100_bases
ATTGTAGGAACATCAGGGCGAATGTAATTCGCCCCTACAAATGGATTTTAAAATTTGTACAATGACAATTTAAAGATATA
AAAATAATTAGGAATATAAG

Downstream 100 bases:

>100_bases
TCCCCATACGAGCCGTATGGGGCTACATAATCCGAGCCACGCTGTGGCTCTTTGATACAGCCGCAGAGCGGCTGAGTTTA
TTCAGCCTAGCACGGCTCGT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MNLVIINTGCANLSSVKFAFDRLGIQAEISCDLDKIKSADKLLLPGVGTAAAAMKILQDRDLIDTIRNATQPMLGICLGM
QLMTEFSAEGDVDTLKLMSGKTELIPNTGLPLPHMGWNKVQYQADHPLFHGIEQNSHFYFVHSYAVLPNENTIATADYGV
PFSAAIASKNFYGVQFHPERSGKNGAQLLRNFVENI

Sequences:

>Translated_196_residues
MNLVIINTGCANLSSVKFAFDRLGIQAEISCDLDKIKSADKLLLPGVGTAAAAMKILQDRDLIDTIRNATQPMLGICLGM
QLMTEFSAEGDVDTLKLMSGKTELIPNTGLPLPHMGWNKVQYQADHPLFHGIEQNSHFYFVHSYAVLPNENTIATADYGV
PFSAAIASKNFYGVQFHPERSGKNGAQLLRNFVENI
>Mature_196_residues
MNLVIINTGCANLSSVKFAFDRLGIQAEISCDLDKIKSADKLLLPGVGTAAAAMKILQDRDLIDTIRNATQPMLGICLGM
QLMTEFSAEGDVDTLKLMSGKTELIPNTGLPLPHMGWNKVQYQADHPLFHGIEQNSHFYFVHSYAVLPNENTIATADYGV
PFSAAIASKNFYGVQFHPERSGKNGAQLLRNFVENI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=195, Percent_Identity=56.4102564102564, Blast_Score=220, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6319725, Length=207, Percent_Identity=32.3671497584541, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21471; Mature: 21471

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLVIINTGCANLSSVKFAFDRLGIQAEISCDLDKIKSADKLLLPGVGTAAAAMKILQDR
CEEEEEECCCCCCHHHHHHHHHCCCEEEEECCHHHHCCCCEEECCCCCHHHHHHHHHHCC
DLIDTIRNATQPMLGICLGMQLMTEFSAEGDVDTLKLMSGKTELIPNTGLPLPHMGWNKV
HHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCCE
QYQADHPLFHGIEQNSHFYFVHSYAVLPNENTIATADYGVPFSAAIASKNFYGVQFHPER
EEECCCCCEECCCCCCEEEEEEEEEEECCCCEEEEECCCCCHHHHHCCCCEEEEEECCCC
SGKNGAQLLRNFVENI
CCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNLVIINTGCANLSSVKFAFDRLGIQAEISCDLDKIKSADKLLLPGVGTAAAAMKILQDR
CEEEEEECCCCCCHHHHHHHHHCCCEEEEECCHHHHCCCCEEECCCCCHHHHHHHHHHCC
DLIDTIRNATQPMLGICLGMQLMTEFSAEGDVDTLKLMSGKTELIPNTGLPLPHMGWNKV
HHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCCE
QYQADHPLFHGIEQNSHFYFVHSYAVLPNENTIATADYGVPFSAAIASKNFYGVQFHPER
EEECCCCCEECCCCCCEEEEEEEEEEECCCCEEEEECCCCCHHHHHCCCCEEEEEECCCC
SGKNGAQLLRNFVENI
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA