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The map label for this gene is mutM

Identifier: 126209444

GI number: 126209444

Start: 2216194

End: 2217015

Strand: Reverse

Name: mutM

Synonym: APL_1990

Alternate gene names: 126209444

Gene position: 2217015-2216194 (Counterclockwise)

Preceding gene: 126209445

Following gene: 126209435

Centisome position: 97.47

GC content: 44.65

Gene sequence:

>822_bases
ATGCCCGAATTACCGGAAGTGGAAACCAGCTTACGAGGTGTAGAACCTTATCTGCACGGCAAAATTATTAAACAAATTGT
CGTACGTACCCAAAAACTGCGTTGGGCGGTTTCGGACGAATTGCAACATATGCAAGGGGCGAAAATCGTTGCATTATCCC
GCCGAGCCAAATATTTGATATTGCATACCACTCAAGGCGATATTTTGATTCACTTGGGTATGTCCGGCTCGTTAGGGATT
TTACAGGAAAACCAACAGCCTGCGGGTAAACACGACCACGTTGATCTCATTACGCAAGACGGTATGGTTTTACGTTATAA
CGATCCGCGTAAATTCGGTTGTTGGCTATGGACGAAAAATGCCGAACAACACGAATTAATTACCCGTTTAGGGCCGGAAC
CGTTATCCGAATCTTTTACCGCTGCGTATTTATTTGCACGAAGCCGTAACAAAACCGTAGCGGTAAAGAACTTTATTATG
AATAACGATATTGTAGTGGGCGTAGGGAATATCTATGCTTGCGAATCGCTGTTTATGGCGGAGCTCCATCCGGAACTTGC
CGCACAGAATCTAACCGAGAAACAGTGCGAACGCTTAGTGAAAGTGATTAAAGAAGTGCTGGCTAAAGCGATTATTCAAG
GCGGTACAACACTGAAAGATTTTATTCAACCGGACGGTAAACCGGGCTATTTCGCCCAAGTATTACAAGTTTACGGACGT
AAAGACGAAGCCTGTAATGATTGCGGCACCATCATTGAAGCGAAAGTTATCGGGCAACGTAACAGCTATTTCTGTCCGCA
TTGCCAAATGCTACCTAGATAG

Upstream 100 bases:

>100_bases
TTGGCGTCGTCTTCAGCCGTTTAGTCAGAGCGCAATAATAAAATCAATCGGGCAAGGTTATCCTTGCCCTTTGTTTATCT
AAATTCTTTGGAGAATATAC

Downstream 100 bases:

>100_bases
CCTAAACGTAAAAAAGCGGTCAAATTTGACCGCTTTTTTGCAACAACTTACTACATAAACCAACCGGTCGGGGTCACCGA
TTTGTTATCGAATAATTTCA

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MPELPEVETSLRGVEPYLHGKIIKQIVVRTQKLRWAVSDELQHMQGAKIVALSRRAKYLILHTTQGDILIHLGMSGSLGI
LQENQQPAGKHDHVDLITQDGMVLRYNDPRKFGCWLWTKNAEQHELITRLGPEPLSESFTAAYLFARSRNKTVAVKNFIM
NNDIVVGVGNIYACESLFMAELHPELAAQNLTEKQCERLVKVIKEVLAKAIIQGGTTLKDFIQPDGKPGYFAQVLQVYGR
KDEACNDCGTIIEAKVIGQRNSYFCPHCQMLPR

Sequences:

>Translated_273_residues
MPELPEVETSLRGVEPYLHGKIIKQIVVRTQKLRWAVSDELQHMQGAKIVALSRRAKYLILHTTQGDILIHLGMSGSLGI
LQENQQPAGKHDHVDLITQDGMVLRYNDPRKFGCWLWTKNAEQHELITRLGPEPLSESFTAAYLFARSRNKTVAVKNFIM
NNDIVVGVGNIYACESLFMAELHPELAAQNLTEKQCERLVKVIKEVLAKAIIQGGTTLKDFIQPDGKPGYFAQVLQVYGR
KDEACNDCGTIIEAKVIGQRNSYFCPHCQMLPR
>Mature_272_residues
PELPEVETSLRGVEPYLHGKIIKQIVVRTQKLRWAVSDELQHMQGAKIVALSRRAKYLILHTTQGDILIHLGMSGSLGIL
QENQQPAGKHDHVDLITQDGMVLRYNDPRKFGCWLWTKNAEQHELITRLGPEPLSESFTAAYLFARSRNKTVAVKNFIMN
NDIVVGVGNIYACESLFMAELHPELAAQNLTEKQCERLVKVIKEVLAKAIIQGGTTLKDFIQPDGKPGYFAQVLQVYGRK
DEACNDCGTIIEAKVIGQRNSYFCPHCQMLPR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=269, Percent_Identity=56.8773234200744, Blast_Score=316, Evalue=9e-88,
Organism=Escherichia coli, GI1786932, Length=281, Percent_Identity=24.1992882562278, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_ACTP2 (A3N3S8)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054669.1
- ProteinModelPortal:   A3N3S8
- SMR:   A3N3S8
- STRING:   A3N3S8
- GeneID:   4850095
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1990
- eggNOG:   COG0266
- HOGENOM:   HBG690070
- OMA:   RMTGQLL
- PhylomeDB:   A3N3S8
- ProtClustDB:   PRK01103
- BioCyc:   APLE416269:APL_1990-MONOMER
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 30737; Mature: 30606

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 57-57 ACT_SITE 260-260 BINDING 91-91 BINDING 110-110 BINDING 151-151

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETSLRGVEPYLHGKIIKQIVVRTQKLRWAVSDELQHMQGAKIVALSRRAKYLI
CCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEE
LHTTQGDILIHLGMSGSLGILQENQQPAGKHDHVDLITQDGMVLRYNDPRKFGCWLWTKN
EEECCCCEEEEECCCCCCCEEECCCCCCCCCCCEEEEECCCEEEEECCCCCEEEEEEECC
AEQHELITRLGPEPLSESFTAAYLFARSRNKTVAVKNFIMNNDIVVGVGNIYACESLFMA
CHHHHHHHHCCCCCHHHHHHHHHEEECCCCCEEEEEEEEECCCEEEECCCHHHHHHHHHH
ELHPELAAQNLTEKQCERLVKVIKEVLAKAIIQGGTTLKDFIQPDGKPGYFAQVLQVYGR
HHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHCC
KDEACNDCGTIIEAKVIGQRNSYFCPHCQMLPR
CCCCHHHHHHEEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PELPEVETSLRGVEPYLHGKIIKQIVVRTQKLRWAVSDELQHMQGAKIVALSRRAKYLI
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEE
LHTTQGDILIHLGMSGSLGILQENQQPAGKHDHVDLITQDGMVLRYNDPRKFGCWLWTKN
EEECCCCEEEEECCCCCCCEEECCCCCCCCCCCEEEEECCCEEEEECCCCCEEEEEEECC
AEQHELITRLGPEPLSESFTAAYLFARSRNKTVAVKNFIMNNDIVVGVGNIYACESLFMA
CHHHHHHHHCCCCCHHHHHHHHHEEECCCCCEEEEEEEEECCCEEEECCCHHHHHHHHHH
ELHPELAAQNLTEKQCERLVKVIKEVLAKAIIQGGTTLKDFIQPDGKPGYFAQVLQVYGR
HHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHCC
KDEACNDCGTIIEAKVIGQRNSYFCPHCQMLPR
CCCCHHHHHHEEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA