Click here to switch to the map view.

The map label for this gene is brnQ [C]

Identifier: 126209445

GI number: 126209445

Start: 2217078

End: 2218370

Strand: Reverse

Name: brnQ [C]

Synonym: APL_1991

Alternate gene names: 126209445

Gene position: 2218370-2217078 (Counterclockwise)

Preceding gene: 126209446

Following gene: 126209444

Centisome position: 97.53

GC content: 42.54

Gene sequence:

>1293_bases
ATGTATATGACTAAAAATACATTTGTGGTCGGCTTTATGCTGTTTGCCATCTTCTTTGGGGCGGGTAACTTAATTTTTCC
GCCGAAATTAGGTTTTGAAAGCGGTGCGGATTTTTGGACCGCAACCATCGGTTTTATTATTACCGGAGTAGGCTTACCGT
TACTCGGTATTATCGTCAGCGCCTCTTATAAAGGCGGCTATAAAGAGGCGTTACATCGTATTCATCCGTGGTTTTCTATC
TTATTCTTAGTTGCGATTTATTTAACTATCGGACCTTTCTTTGCCATTCCGCGTACGGCGGCAACCGCTTATGAAATGGC
GGTATTACCGTTTTTAGGCGAGCAAACCACCGTATCGTTACTGATTTTTACCGCAGTATATTTTGCTTTAACCATTTGGC
TAAGTTTAAATCCGTCCAAAATGGTGGAGCGAATCGGTTCTATCCTGACACCGGCATTACTCATCGCCATTCTTGCACTT
GTCATTAGCGGTATCGCATTATTCTCCGATAATCCGTCAAGCAGCAATGTGATGGAAGCGCCGATGGTAAACGGCATCCT
TGCCGGTTACAATACGATGGACGCTTTAGCTTCCGTTGCCTTTTCGGTTATCGTAATCAATGCGATTAAAAGCAAAGGCT
TATCCGAAACCAAAGCATTAAGTAAACAAACCGCAATGGCGGGGGTAATCGCTGCAATTTTATTAGCGTTAATTTATCTC
TCTATCGGTTGGATCGGTAACAAATTACCGATTTCTGCGGAAGTATTGGCGGACTTAACCGCTAAAAAACAAGATTTAGG
GACTTATATTCTTAACGTTGCCGCAACGCAAGCCTTCGGCGAATTAGGTAGAACGCTATTAGGCGTTATCGTATCGCTTG
CTTGTTTAACTACCGCTATCGGTCTTGTCGTATCGGTAAGCGAATATTTCCACGAAATTTTCCCGAAAATTTCTTATAGA
ACCTATGCAATCGTTTGTACTTTAATCGGTTTCGGCTTGGCAAACCAAGGGCTAAGTGCGGTAATCAGTAAATCTATTCC
GGTATTACTGGTACTTTATCCGATTGCTATGTCGATTATTTTCTTACTGTTATTGAATCTATTCGTGAAACTCCCGTTAT
TAGCATTACGTGTTTCACTGATTCTCGTGACAATCGTATCCATTCTTTCCGTAGCAGGCGTAAGTATTACCGAAAACTTA
CCGCTTAAAGCCTACTCTATGGAATGGCTACCGTTTGCCGTAGGCGGATTACTGATTGGCGTCGTCTTCAGCCGTTTAGT
CAGAGCGCAATAA

Upstream 100 bases:

>100_bases
CGAGCTTTACTTTTCGGTAAAGTTCGGTTTTACTATTTTTTCGGGTCTCCTTTTTCTTTTTATATTTGTTATTTGCTGAG
AAGCAAATTAAAAGTGTGGT

Downstream 100 bases:

>100_bases
TAAAATCAATCGGGCAAGGTTATCCTTGCCCTTTGTTTATCTAAATTCTTTGGAGAATATACATGCCCGAATTACCGGAA
GTGGAAACCAGCTTACGAGG

Product: branched chain amino acid ABC transporter carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MYMTKNTFVVGFMLFAIFFGAGNLIFPPKLGFESGADFWTATIGFIITGVGLPLLGIIVSASYKGGYKEALHRIHPWFSI
LFLVAIYLTIGPFFAIPRTAATAYEMAVLPFLGEQTTVSLLIFTAVYFALTIWLSLNPSKMVERIGSILTPALLIAILAL
VISGIALFSDNPSSSNVMEAPMVNGILAGYNTMDALASVAFSVIVINAIKSKGLSETKALSKQTAMAGVIAAILLALIYL
SIGWIGNKLPISAEVLADLTAKKQDLGTYILNVAATQAFGELGRTLLGVIVSLACLTTAIGLVVSVSEYFHEIFPKISYR
TYAIVCTLIGFGLANQGLSAVISKSIPVLLVLYPIAMSIIFLLLLNLFVKLPLLALRVSLILVTIVSILSVAGVSITENL
PLKAYSMEWLPFAVGGLLIGVVFSRLVRAQ

Sequences:

>Translated_430_residues
MYMTKNTFVVGFMLFAIFFGAGNLIFPPKLGFESGADFWTATIGFIITGVGLPLLGIIVSASYKGGYKEALHRIHPWFSI
LFLVAIYLTIGPFFAIPRTAATAYEMAVLPFLGEQTTVSLLIFTAVYFALTIWLSLNPSKMVERIGSILTPALLIAILAL
VISGIALFSDNPSSSNVMEAPMVNGILAGYNTMDALASVAFSVIVINAIKSKGLSETKALSKQTAMAGVIAAILLALIYL
SIGWIGNKLPISAEVLADLTAKKQDLGTYILNVAATQAFGELGRTLLGVIVSLACLTTAIGLVVSVSEYFHEIFPKISYR
TYAIVCTLIGFGLANQGLSAVISKSIPVLLVLYPIAMSIIFLLLLNLFVKLPLLALRVSLILVTIVSILSVAGVSITENL
PLKAYSMEWLPFAVGGLLIGVVFSRLVRAQ
>Mature_430_residues
MYMTKNTFVVGFMLFAIFFGAGNLIFPPKLGFESGADFWTATIGFIITGVGLPLLGIIVSASYKGGYKEALHRIHPWFSI
LFLVAIYLTIGPFFAIPRTAATAYEMAVLPFLGEQTTVSLLIFTAVYFALTIWLSLNPSKMVERIGSILTPALLIAILAL
VISGIALFSDNPSSSNVMEAPMVNGILAGYNTMDALASVAFSVIVINAIKSKGLSETKALSKQTAMAGVIAAILLALIYL
SIGWIGNKLPISAEVLADLTAKKQDLGTYILNVAATQAFGELGRTLLGVIVSLACLTTAIGLVVSVSEYFHEIFPKISYR
TYAIVCTLIGFGLANQGLSAVISKSIPVLLVLYPIAMSIIFLLLLNLFVKLPLLALRVSLILVTIVSILSVAGVSITENL
PLKAYSMEWLPFAVGGLLIGVVFSRLVRAQ

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine), which is coupled to a proton motive force (Potential) [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=363, Percent_Identity=36.0881542699724, Blast_Score=221, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 45944; Mature: 45944

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYMTKNTFVVGFMLFAIFFGAGNLIFPPKLGFESGADFWTATIGFIITGVGLPLLGIIVS
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ASYKGGYKEALHRIHPWFSILFLVAIYLTIGPFFAIPRTAATAYEMAVLPFLGEQTTVSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHH
LIFTAVYFALTIWLSLNPSKMVERIGSILTPALLIAILALVISGIALFSDNPSSSNVMEA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
PMVNGILAGYNTMDALASVAFSVIVINAIKSKGLSETKALSKQTAMAGVIAAILLALIYL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SIGWIGNKLPISAEVLADLTAKKQDLGTYILNVAATQAFGELGRTLLGVIVSLACLTTAI
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVVSVSEYFHEIFPKISYRTYAIVCTLIGFGLANQGLSAVISKSIPVLLVLYPIAMSII
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FLLLLNLFVKLPLLALRVSLILVTIVSILSVAGVSITENLPLKAYSMEWLPFAVGGLLIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEECCCHHHHHHHHHHHH
VVFSRLVRAQ
HHHHHHHCCC
>Mature Secondary Structure
MYMTKNTFVVGFMLFAIFFGAGNLIFPPKLGFESGADFWTATIGFIITGVGLPLLGIIVS
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ASYKGGYKEALHRIHPWFSILFLVAIYLTIGPFFAIPRTAATAYEMAVLPFLGEQTTVSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHH
LIFTAVYFALTIWLSLNPSKMVERIGSILTPALLIAILALVISGIALFSDNPSSSNVMEA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
PMVNGILAGYNTMDALASVAFSVIVINAIKSKGLSETKALSKQTAMAGVIAAILLALIYL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SIGWIGNKLPISAEVLADLTAKKQDLGTYILNVAATQAFGELGRTLLGVIVSLACLTTAI
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVVSVSEYFHEIFPKISYRTYAIVCTLIGFGLANQGLSAVISKSIPVLLVLYPIAMSII
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FLLLLNLFVKLPLLALRVSLILVTIVSILSVAGVSITENLPLKAYSMEWLPFAVGGLLIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEECCCHHHHHHHHHHHH
VVFSRLVRAQ
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA