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The map label for this gene is hemB [H]

Identifier: 126209442

GI number: 126209442

Start: 2214675

End: 2215694

Strand: Direct

Name: hemB [H]

Synonym: APL_1988

Alternate gene names: 126209442

Gene position: 2214675-2215694 (Clockwise)

Preceding gene: 126209441

Following gene: 126209443

Centisome position: 97.37

GC content: 43.63

Gene sequence:

>1020_bases
ATGACTCAATATCTTAACACTTCATTTCCCACTCGCCGTATGCGTCGTTTACGTAAACATGATTTCAGCCGTCGTTTAGT
GGCGGAAAGTCAATTGACTGCCGGCGATTTAATTTATCCGGTATTTGTGATTGAAGGCGAAAATCAGCGAGTAAAAGTAC
CGTCTATGCCGAGTGTAGAACGTTTAACCATCGATCAATTATTAATTGAAGCCGGCGAATTGGTCAAATACGGCGTACCT
GCAATAGCTCTTTTCCCAGTAGTAGGTAATGAGAAAAAATCTCTGATGGCGGAAGAAGCGTATAATCCGGAAGGTTTGGC
ACAGCGTGCAGTACGTGCATTAAAAGCGGCTTATCCTGAATTAGGCGTGATGACTGATGTGGCGTTAGATCCATTCACTA
CACACGGTCAAGATGGCATTATTGATGAAGAAGGTTATGTACTGAATGACATCACAACCGAAGTATTAGTAAAACAAGCG
GTATCTCACGCAGAAGCTGGTGCGGATATTGTTGCACCAAGCGATATGATGGACGGTCGTATCGGTAAAATTCGTGAAGC
ACTTGAAGCGAAAGGCTTAATTAATACGCAAATTATGGCGTATTCGGCGAAATATGCGTCAAATTATTACGGGCCGTTCC
GTGATGCGGTCGGTTCGGCGGGTAACTTAAAAGGCGGTAATAAATTTACTTATCAGGTTGATCCGGCGAATGCGAACGAA
GGTTTACACGAAGTGGCAATGGATATTCAAGAAGGGGCGGATATGGTGATGGTAAAACCGGGTATGCCTTATTTGGATAT
GGTATGGCGTGTAAAAGAAACCTTTGGCGTACCGACTTTTGCTTATCAAGTATCCGGTGAATACGCAATGCATATGGCGG
CGATTCAAAACGGCTGGTTAAAAGAACGTGAGTGCGTTATGGAAGGTTTACTTTGCTTTAAACGTGCGGGAGCGGACGGT
ATTTTAACCTATTTCGCTAAAACGGTAGCGAAATGGCTGTACGAAGATAATCGTAAATAA

Upstream 100 bases:

>100_bases
ATAAACCGAGAGACGAGCAACCGGAAACGATCGCCAATTAATCCAACCCGCTTGCAAGCGGGTTATTTTTTATGAAAATT
TACCAAATTAGGAAGCAATT

Downstream 100 bases:

>100_bases
CGATAAGAGTTAAATGTAAGCGGTTAGATTGAATGAATTTTTTGTAAAACATTCGGTCTAACCGTTACTTTTTTAGGAGG
TATAAAATGAACGACTCTTT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTQYLNTSFPTRRMRRLRKHDFSRRLVAESQLTAGDLIYPVFVIEGENQRVKVPSMPSVERLTIDQLLIEAGELVKYGVP
AIALFPVVGNEKKSLMAEEAYNPEGLAQRAVRALKAAYPELGVMTDVALDPFTTHGQDGIIDEEGYVLNDITTEVLVKQA
VSHAEAGADIVAPSDMMDGRIGKIREALEAKGLINTQIMAYSAKYASNYYGPFRDAVGSAGNLKGGNKFTYQVDPANANE
GLHEVAMDIQEGADMVMVKPGMPYLDMVWRVKETFGVPTFAYQVSGEYAMHMAAIQNGWLKERECVMEGLLCFKRAGADG
ILTYFAKTVAKWLYEDNRK

Sequences:

>Translated_339_residues
MTQYLNTSFPTRRMRRLRKHDFSRRLVAESQLTAGDLIYPVFVIEGENQRVKVPSMPSVERLTIDQLLIEAGELVKYGVP
AIALFPVVGNEKKSLMAEEAYNPEGLAQRAVRALKAAYPELGVMTDVALDPFTTHGQDGIIDEEGYVLNDITTEVLVKQA
VSHAEAGADIVAPSDMMDGRIGKIREALEAKGLINTQIMAYSAKYASNYYGPFRDAVGSAGNLKGGNKFTYQVDPANANE
GLHEVAMDIQEGADMVMVKPGMPYLDMVWRVKETFGVPTFAYQVSGEYAMHMAAIQNGWLKERECVMEGLLCFKRAGADG
ILTYFAKTVAKWLYEDNRK
>Mature_338_residues
TQYLNTSFPTRRMRRLRKHDFSRRLVAESQLTAGDLIYPVFVIEGENQRVKVPSMPSVERLTIDQLLIEAGELVKYGVPA
IALFPVVGNEKKSLMAEEAYNPEGLAQRAVRALKAAYPELGVMTDVALDPFTTHGQDGIIDEEGYVLNDITTEVLVKQAV
SHAEAGADIVAPSDMMDGRIGKIREALEAKGLINTQIMAYSAKYASNYYGPFRDAVGSAGNLKGGNKFTYQVDPANANEG
LHEVAMDIQEGADMVMVKPGMPYLDMVWRVKETFGVPTFAYQVSGEYAMHMAAIQNGWLKERECVMEGLLCFKRAGADGI
LTYFAKTVAKWLYEDNRK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=312, Percent_Identity=40.0641025641026, Blast_Score=223, Evalue=1e-58,
Organism=Escherichia coli, GI87081728, Length=320, Percent_Identity=42.5, Blast_Score=222, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6321398, Length=328, Percent_Identity=35.0609756097561, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI21358291, Length=309, Percent_Identity=36.8932038834951, Blast_Score=208, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 37582; Mature: 37450

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQYLNTSFPTRRMRRLRKHDFSRRLVAESQLTAGDLIYPVFVIEGENQRVKVPSMPSVE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEECCCCCCHH
RLTIDQLLIEAGELVKYGVPAIALFPVVGNEKKSLMAEEAYNPEGLAQRAVRALKAAYPE
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
LGVMTDVALDPFTTHGQDGIIDEEGYVLNDITTEVLVKQAVSHAEAGADIVAPSDMMDGR
CCCHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEECCCHHHCCH
IGKIREALEAKGLINTQIMAYSAKYASNYYGPFRDAVGSAGNLKGGNKFTYQVDPANANE
HHHHHHHHHHCCCHHHHHHEEEHHHHHCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCCC
GLHEVAMDIQEGADMVMVKPGMPYLDMVWRVKETFGVPTFAYQVSGEYAMHMAAIQNGWL
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCH
KERECVMEGLLCFKRAGADGILTYFAKTVAKWLYEDNRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQYLNTSFPTRRMRRLRKHDFSRRLVAESQLTAGDLIYPVFVIEGENQRVKVPSMPSVE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEECCCCCCHH
RLTIDQLLIEAGELVKYGVPAIALFPVVGNEKKSLMAEEAYNPEGLAQRAVRALKAAYPE
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
LGVMTDVALDPFTTHGQDGIIDEEGYVLNDITTEVLVKQAVSHAEAGADIVAPSDMMDGR
CCCHHHCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEECCCHHHCCH
IGKIREALEAKGLINTQIMAYSAKYASNYYGPFRDAVGSAGNLKGGNKFTYQVDPANANE
HHHHHHHHHHCCCHHHHHHEEEHHHHHCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCCC
GLHEVAMDIQEGADMVMVKPGMPYLDMVWRVKETFGVPTFAYQVSGEYAMHMAAIQNGWL
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCH
KERECVMEGLLCFKRAGADGILTYFAKTVAKWLYEDNRK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]