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The map label for this gene is yifE [C]

Identifier: 126209432

GI number: 126209432

Start: 2205462

End: 2205794

Strand: Direct

Name: yifE [C]

Synonym: APL_1978

Alternate gene names: 126209432

Gene position: 2205462-2205794 (Clockwise)

Preceding gene: 126209429

Following gene: 126209433

Centisome position: 96.97

GC content: 43.54

Gene sequence:

>333_bases
ATGGCAGAGAGTTTTAGCGTTACGCGTCGTTTTTTTGATGATAAAAATTATCCGCGCGGTTTTGCACGCCACGGCGATTA
CACTATTCGTGAAGCACAAACTTTAGAGCAATTCGGTCAGGCGTGTTTAGCGTTAGAATCAGGTGAGCGTAGCCCGGTAA
CGGCTGAAGAACAACGTTTCGTTGCCGTAATGAAAGGTGATGAAATTGCTGAAAGCATTATTGAAAAATCATGGTTAAAA
TACCGTTCATTAACCAGCAAAACCAAACGTATTTATACGCTTTCGGGTAATGCGGGTAACGATGCCGGTGATGATTTCAG
CGCGGCTGAATAA

Upstream 100 bases:

>100_bases
ATACTTATTTTTTCTTGACTTATTTTGAATAATTGCTTTAATGGTAGGTCGATTTTTTTCTTTCTAAATTCAGCAATAAA
TAATTAATAAGAGGCATATT

Downstream 100 bases:

>100_bases
TCGGTATTTCTGAGCATTTGCAGTTTTAATGATGCCACGAAATACGCTGAAGACACATAAGTGTCGGTAGGGGTATTTTG
TGGTTTCTTTTTTTCTACTA

Product: hypothetical protein

Products: NA

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 110; Mature: 109

Protein sequence:

>110_residues
MAESFSVTRRFFDDKNYPRGFARHGDYTIREAQTLEQFGQACLALESGERSPVTAEEQRFVAVMKGDEIAESIIEKSWLK
YRSLTSKTKRIYTLSGNAGNDAGDDFSAAE

Sequences:

>Translated_110_residues
MAESFSVTRRFFDDKNYPRGFARHGDYTIREAQTLEQFGQACLALESGERSPVTAEEQRFVAVMKGDEIAESIIEKSWLK
YRSLTSKTKRIYTLSGNAGNDAGDDFSAAE
>Mature_109_residues
AESFSVTRRFFDDKNYPRGFARHGDYTIREAQTLEQFGQACLALESGERSPVTAEEQRFVAVMKGDEIAESIIEKSWLKY
RSLTSKTKRIYTLSGNAGNDAGDDFSAAE

Specific function: Not known, a mutation in ORF3 causes a slight decrease in transformation efficiency [H]

COG id: COG3085

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0438 family [H]

Homologues:

Organism=Escherichia coli, GI2367277, Length=96, Percent_Identity=53.125, Blast_Score=107, Evalue=1e-25,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007335 [H]

Pfam domain/function: PF04219 DUF413 [H]

EC number: NA

Molecular weight: Translated: 12369; Mature: 12238

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESFSVTRRFFDDKNYPRGFARHGDYTIREAQTLEQFGQACLALESGERSPVTAEEQRF
CCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHE
VAVMKGDEIAESIIEKSWLKYRSLTSKTKRIYTLSGNAGNDAGDDFSAAE
EEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
AESFSVTRRFFDDKNYPRGFARHGDYTIREAQTLEQFGQACLALESGERSPVTAEEQRF
CCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHE
VAVMKGDEIAESIIEKSWLKYRSLTSKTKRIYTLSGNAGNDAGDDFSAAE
EEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1438213; 7542800; 10675023 [H]