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The map label for this gene is trmA

Identifier: 126209433

GI number: 126209433

Start: 2205957

End: 2207063

Strand: Direct

Name: trmA

Synonym: APL_1979

Alternate gene names: 126209433

Gene position: 2205957-2207063 (Clockwise)

Preceding gene: 126209432

Following gene: 126209436

Centisome position: 96.99

GC content: 41.28

Gene sequence:

>1107_bases
ATGAATCTTCCTATTGAGCAATATGCCGATCTTCTTGCAGAAAAAGCGAAAAATTTGACCGCTTTACTTGCGCCTTTTAA
TCCGCCTGAGCTTGAAGTATTTGAGTCTGAAACCGGCCATTTCCGTATGCGTGCCGAATTCCGAGTATGGCATGATACTA
ACGAAGTCGGAGAAAACGAGCTGTATCACATTATGTTCGATCAGGAAACCAAGCAACGTTATTGTGTTGAACAATTCCCG
ATTGCCAATCATTTAATTAATAAAATGATGAGCAGCTTATTAGCTGAAATTAAGGGTAATGAATTACTCACTCGTAAATT
ATTCCAAGTGGATTACCTCAGCACGTTAAGCGGTGAAATTGCGGTTTCAATGCTTTATCACAAAAAGCTAAACGAAGAAT
GGCAAGCGGAAGCCGCCGCATTAAAAGCTCGTTTGGAACATCAGGGTTTTAAGGTACAAATTATCGGACGAGCGACAAAA
CAGAAGATTGCTTTAGATCGTGATTATGTGGAAGAAGTATTGCCGGTGGACGGGCGTAATTTAATTTATCGCCAAGTAGA
AAACAGCTTTACCCAGCCGAATGCCAAAATGAATATCAAGATGTTGGAATGGGCGAGAAGCTGTACTCGACACAGTAGCG
GCGATTTATTAGAACTTTACTGCGGTAACGGGAATTTTTCGATTGCATTAGCGGAGAACTTCCGCAAAGTGTTAGCTACC
GAGATTTCAAAATCTTCGGTGCAATCGGCGCAATATAATATTGAGCAAAACGGGATCGATAACTTGCAAATTATTCGAAT
GTCGGCGGAAGAATTTACTCAAGCGATGAACGGCGTGCGTGAGTTTAATCGTTTGAAAGGGATTGATTTAAAAGCCTATG
ATTGCAATACGATTTTTGTGGATCCGCCGCGTGCGGGGTTAGACCAAGATACCTTAAATATGGTACAGGCTTACGAACGA
ATTTTATATATTTCGTGTAATCCGTATACGTTGGCGGAGAATTTACGACAATTATCTCTAACGCATCGTATCGAACGTGC
GGCACTTTTTGATCAGTTCCCTTATACGCATCACGTTGAAAGCGGTGTATGGCTGATTAGGAAATAA

Upstream 100 bases:

>100_bases
GTCGGTAGGGGTATTTTGTGGTTTCTTTTTTTCTACTATTTTTCTACTATTTTAAATTTGCCGTCTTTTAGTCGGCAGGC
AAAATGAACCCTGATAAATT

Downstream 100 bases:

>100_bases
GCATAAATAGTCGGTTATGCTGAAAAATAAATCATTTAGCGATTTTTGTAGATTTTTCGCTTGACCGATTTAGCTAAAAC
TTATATTATTCCGCTCGTTT

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MNLPIEQYADLLAEKAKNLTALLAPFNPPELEVFESETGHFRMRAEFRVWHDTNEVGENELYHIMFDQETKQRYCVEQFP
IANHLINKMMSSLLAEIKGNELLTRKLFQVDYLSTLSGEIAVSMLYHKKLNEEWQAEAAALKARLEHQGFKVQIIGRATK
QKIALDRDYVEEVLPVDGRNLIYRQVENSFTQPNAKMNIKMLEWARSCTRHSSGDLLELYCGNGNFSIALAENFRKVLAT
EISKSSVQSAQYNIEQNGIDNLQIIRMSAEEFTQAMNGVREFNRLKGIDLKAYDCNTIFVDPPRAGLDQDTLNMVQAYER
ILYISCNPYTLAENLRQLSLTHRIERAALFDQFPYTHHVESGVWLIRK

Sequences:

>Translated_368_residues
MNLPIEQYADLLAEKAKNLTALLAPFNPPELEVFESETGHFRMRAEFRVWHDTNEVGENELYHIMFDQETKQRYCVEQFP
IANHLINKMMSSLLAEIKGNELLTRKLFQVDYLSTLSGEIAVSMLYHKKLNEEWQAEAAALKARLEHQGFKVQIIGRATK
QKIALDRDYVEEVLPVDGRNLIYRQVENSFTQPNAKMNIKMLEWARSCTRHSSGDLLELYCGNGNFSIALAENFRKVLAT
EISKSSVQSAQYNIEQNGIDNLQIIRMSAEEFTQAMNGVREFNRLKGIDLKAYDCNTIFVDPPRAGLDQDTLNMVQAYER
ILYISCNPYTLAENLRQLSLTHRIERAALFDQFPYTHHVESGVWLIRK
>Mature_368_residues
MNLPIEQYADLLAEKAKNLTALLAPFNPPELEVFESETGHFRMRAEFRVWHDTNEVGENELYHIMFDQETKQRYCVEQFP
IANHLINKMMSSLLAEIKGNELLTRKLFQVDYLSTLSGEIAVSMLYHKKLNEEWQAEAAALKARLEHQGFKVQIIGRATK
QKIALDRDYVEEVLPVDGRNLIYRQVENSFTQPNAKMNIKMLEWARSCTRHSSGDLLELYCGNGNFSIALAENFRKVLAT
EISKSSVQSAQYNIEQNGIDNLQIIRMSAEEFTQAMNGVREFNRLKGIDLKAYDCNTIFVDPPRAGLDQDTLNMVQAYER
ILYISCNPYTLAENLRQLSLTHRIERAALFDQFPYTHHVESGVWLIRK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily

Homologues:

Organism=Homo sapiens, GI34222389, Length=216, Percent_Identity=26.3888888888889, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI51173878, Length=216, Percent_Identity=26.3888888888889, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1790403, Length=367, Percent_Identity=64.0326975476839, Blast_Score=496, Evalue=1e-142,
Organism=Escherichia coli, GI1789148, Length=188, Percent_Identity=31.9148936170213, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787083, Length=184, Percent_Identity=27.7173913043478, Blast_Score=78, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24666579, Length=161, Percent_Identity=31.6770186335404, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRMA_ACTP2 (A3N3R7)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054658.1
- ProteinModelPortal:   A3N3R7
- SMR:   A3N3R7
- STRING:   A3N3R7
- GeneID:   4848598
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1979
- eggNOG:   COG2265
- HOGENOM:   HBG608402
- OMA:   NEVRPFR
- PhylomeDB:   A3N3R7
- ProtClustDB:   PRK05031
- BioCyc:   APLE416269:APL_1979-MONOMER
- HAMAP:   MF_01011
- InterPro:   IPR011869
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02143

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.35

Molecular weight: Translated: 42491; Mature: 42491

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 326-326 BINDING 192-192 BINDING 220-220 BINDING 225-225 BINDING 241-241 BINDING 301-301

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPIEQYADLLAEKAKNLTALLAPFNPPELEVFESETGHFRMRAEFRVWHDTNEVGENE
CCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCCCEEEEEEEEEEECCCCCCCCC
LYHIMFDQETKQRYCVEQFPIANHLINKMMSSLLAEIKGNELLTRKLFQVDYLSTLSGEI
EEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHH
AVSMLYHKKLNEEWQAEAAALKARLEHQGFKVQIIGRATKQKIALDRDYVEEVLPVDGRN
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCHHHHHHHCCCCCCH
LIYRQVENSFTQPNAKMNIKMLEWARSCTRHSSGDLLELYCGNGNFSIALAENFRKVLAT
HHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEHHHHHHHHHH
EISKSSVQSAQYNIEQNGIDNLQIIRMSAEEFTQAMNGVREFNRLKGIDLKAYDCNTIFV
HHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEE
DPPRAGLDQDTLNMVQAYERILYISCNPYTLAENLRQLSLTHRIERAALFDQFPYTHHVE
CCCCCCCCHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
SGVWLIRK
CCEEEEEC
>Mature Secondary Structure
MNLPIEQYADLLAEKAKNLTALLAPFNPPELEVFESETGHFRMRAEFRVWHDTNEVGENE
CCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCCCEEEEEEEEEEECCCCCCCCC
LYHIMFDQETKQRYCVEQFPIANHLINKMMSSLLAEIKGNELLTRKLFQVDYLSTLSGEI
EEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHH
AVSMLYHKKLNEEWQAEAAALKARLEHQGFKVQIIGRATKQKIALDRDYVEEVLPVDGRN
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCHHHHHHHCCCCCCH
LIYRQVENSFTQPNAKMNIKMLEWARSCTRHSSGDLLELYCGNGNFSIALAENFRKVLAT
HHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEHHHHHHHHHH
EISKSSVQSAQYNIEQNGIDNLQIIRMSAEEFTQAMNGVREFNRLKGIDLKAYDCNTIFV
HHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEE
DPPRAGLDQDTLNMVQAYERILYISCNPYTLAENLRQLSLTHRIERAALFDQFPYTHHVE
CCCCCCCCHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECC
SGVWLIRK
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA