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The map label for this gene is glnA [H]

Identifier: 126209399

GI number: 126209399

Start: 2172693

End: 2174108

Strand: Reverse

Name: glnA [H]

Synonym: APL_1941

Alternate gene names: 126209399

Gene position: 2174108-2172693 (Counterclockwise)

Preceding gene: 126209400

Following gene: 126209395

Centisome position: 95.59

GC content: 43.43

Gene sequence:

>1416_bases
ATGTCTGTAAAAAGAGTATTTGATTTAATCAAAGATAACGATATCAAATTCGTAATGTTAAAATTTACCGATATCAAAGG
TAAAGAACACGGTGTTTCACTCCCGGTAAGTTTAATCGGCGACGATTTAGAAGACTTATTTGAAGAAGGTAAAATGTTCG
ACGGTTCGTCTGTTGAAGGTTGGAAAGCAATCAATAAAGCCGATATGCTTTTAATGCCAATCGCCGAAACCGCTGTTGTT
GATCCTTTCGCTCGAATCCCTACTCTTACTCTCCGCTGTAGCATCTACGATCCAAACACAATGCAATCTTACGACCGCGA
CCCGCGTTCAATCGCAATCCGTGCGGAAAACTACCTGAAATCAACCGGTATCGCCGATAGCGTAATGTTTGGTCCCGAGC
CTGAATTCTTCTTATTTGACGATGTGCGTTACAACATTGGTATGAACAACGTATCGTATAAAATTGATGATATTGAAGCG
GCTTGGAACACGAATCGTAAATATGAAGACGGCAATACAGGCTATCGCCCGCTTAAAAAAGGCGGTTACTGTGCGGTTGC
TCCGATTGATTCGGCACACGATATTCGTTCGGAAATGTGCCTAATTTTAGAAGAAATGGGCTTAGTGGTTGAAGCGCACC
ACCACGAAGTGGCGACGGCAGGTCAAAATGAAATTGCGACGAAATTTAACAGCTTAACGCTTAAAGCGGACGAAACGCAG
ATCTATAAATATGTGGTGCAAAACGTTGCGTTAGAACACGGCAAAACCGCTTGCTTTATGCCGAAACCGTTTGCAGGCGA
CAACGGTTCGGGAATGCACTGTAATATGTCATTAAGCAAAGACGGCAAAAACGTATTTATGGGTGATAAATATGCAGGTT
TATCTGAAACGGCACTTTATTATATCGGCGGTATCATTAAACACGCTAAAGCATTAAATGCGTTTACCAACCCAAGTACA
AACTCGTATAAACGTTTAGTACCGGGCTTTGAAGCGCCGGTGTTATTAGCTTATTCGGCAAGTAACCGTTCGGCATCAAT
CCGTATCCCGGCAGTGACCAGCCCGAAAGCAACCCGTGTTGAAGCGCGCTTCCCGGATCCGTTAGCAAACCCGTACCTCT
GCTTCGCCGCATTATTAATGGCGGGCTTAGACGGTGTAGTAAATAAAATCCACCCGGGCGATGCAATGGATAAAAACCTT
TATGACTTACCGCCGGAAGAACTTAAAGAAATTCCGGCAGTAGCGACTTCATTAGAGGAAGCGTTGGATTCGCTTGCAGC
TGACTATGAATTCTTAACCCAAGGCGGCGTATTTACCAAAGAATTTGTTGAAGCTTATATCAACATTCGTCGTAAAGAAG
TAGAACGTTTAAATATGACACCGCACCCGGTAGAATTTGAAATGTACTACGCATAA

Upstream 100 bases:

>100_bases
AAATTATTACATTTGTCTATTTAAAATTTCCTCAAATTCTTGTATCTTTCTTCTTTCGCAAATTCCTGTGAAAATCAACC
CGCTTGAAGGAAGTCCCACA

Downstream 100 bases:

>100_bases
TTGCAAAAAGATCAAAAAAGGCGACCGCTTGGTCGCCTTTTGTTTGCCTCTTACTTCTCTTTATTGCTTTCTTTATGATT
ACAACAAGGCGGGACGGGAT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSVKRVFDLIKDNDIKFVMLKFTDIKGKEHGVSLPVSLIGDDLEDLFEEGKMFDGSSVEGWKAINKADMLLMPIAETAVV
DPFARIPTLTLRCSIYDPNTMQSYDRDPRSIAIRAENYLKSTGIADSVMFGPEPEFFLFDDVRYNIGMNNVSYKIDDIEA
AWNTNRKYEDGNTGYRPLKKGGYCAVAPIDSAHDIRSEMCLILEEMGLVVEAHHHEVATAGQNEIATKFNSLTLKADETQ
IYKYVVQNVALEHGKTACFMPKPFAGDNGSGMHCNMSLSKDGKNVFMGDKYAGLSETALYYIGGIIKHAKALNAFTNPST
NSYKRLVPGFEAPVLLAYSASNRSASIRIPAVTSPKATRVEARFPDPLANPYLCFAALLMAGLDGVVNKIHPGDAMDKNL
YDLPPEELKEIPAVATSLEEALDSLAADYEFLTQGGVFTKEFVEAYINIRRKEVERLNMTPHPVEFEMYYA

Sequences:

>Translated_471_residues
MSVKRVFDLIKDNDIKFVMLKFTDIKGKEHGVSLPVSLIGDDLEDLFEEGKMFDGSSVEGWKAINKADMLLMPIAETAVV
DPFARIPTLTLRCSIYDPNTMQSYDRDPRSIAIRAENYLKSTGIADSVMFGPEPEFFLFDDVRYNIGMNNVSYKIDDIEA
AWNTNRKYEDGNTGYRPLKKGGYCAVAPIDSAHDIRSEMCLILEEMGLVVEAHHHEVATAGQNEIATKFNSLTLKADETQ
IYKYVVQNVALEHGKTACFMPKPFAGDNGSGMHCNMSLSKDGKNVFMGDKYAGLSETALYYIGGIIKHAKALNAFTNPST
NSYKRLVPGFEAPVLLAYSASNRSASIRIPAVTSPKATRVEARFPDPLANPYLCFAALLMAGLDGVVNKIHPGDAMDKNL
YDLPPEELKEIPAVATSLEEALDSLAADYEFLTQGGVFTKEFVEAYINIRRKEVERLNMTPHPVEFEMYYA
>Mature_470_residues
SVKRVFDLIKDNDIKFVMLKFTDIKGKEHGVSLPVSLIGDDLEDLFEEGKMFDGSSVEGWKAINKADMLLMPIAETAVVD
PFARIPTLTLRCSIYDPNTMQSYDRDPRSIAIRAENYLKSTGIADSVMFGPEPEFFLFDDVRYNIGMNNVSYKIDDIEAA
WNTNRKYEDGNTGYRPLKKGGYCAVAPIDSAHDIRSEMCLILEEMGLVVEAHHHEVATAGQNEIATKFNSLTLKADETQI
YKYVVQNVALEHGKTACFMPKPFAGDNGSGMHCNMSLSKDGKNVFMGDKYAGLSETALYYIGGIIKHAKALNAFTNPSTN
SYKRLVPGFEAPVLLAYSASNRSASIRIPAVTSPKATRVEARFPDPLANPYLCFAALLMAGLDGVVNKIHPGDAMDKNLY
DLPPEELKEIPAVATSLEEALDSLAADYEFLTQGGVFTKEFVEAYINIRRKEVERLNMTPHPVEFEMYYA

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=471, Percent_Identity=69.2144373673036, Blast_Score=699, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=175, Percent_Identity=33.1428571428571, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52324; Mature: 52193

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKRVFDLIKDNDIKFVMLKFTDIKGKEHGVSLPVSLIGDDLEDLFEEGKMFDGSSVEG
CCHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCHHHHCCCHHHHHHCCCCCCCCCCCH
WKAINKADMLLMPIAETAVVDPFARIPTLTLRCSIYDPNTMQSYDRDPRSIAIRAENYLK
HHHCCCCCEEEEECHHHHHHCCHHHCCEEEEEEEEECCCHHHHHCCCCCEEEEEEHHHHH
STGIADSVMFGPEPEFFLFDDVRYNIGMNNVSYKIDDIEAAWNTNRKYEDGNTGYRPLKK
HCCCCCCEEECCCCCEEEEECEEEEECCCCCEEEEECCHHHCCCCCCCCCCCCCCCCCCC
GGYCAVAPIDSAHDIRSEMCLILEEMGLVVEAHHHEVATAGQNEIATKFNSLTLKADETQ
CCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCHHHHHHHCCEEEECCHHH
IYKYVVQNVALEHGKTACFMPKPFAGDNGSGMHCNMSLSKDGKNVFMGDKYAGLSETALY
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCHHHHH
YIGGIIKHAKALNAFTNPSTNSYKRLVPGFEAPVLLAYSASNRSASIRIPAVTSPKATRV
HHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCEE
EARFPDPLANPYLCFAALLMAGLDGVVNKIHPGDAMDKNLYDLPPEELKEIPAVATSLEE
ECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ALDSLAADYEFLTQGGVFTKEFVEAYINIRRKEVERLNMTPHPVEFEMYYA
HHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
>Mature Secondary Structure 
SVKRVFDLIKDNDIKFVMLKFTDIKGKEHGVSLPVSLIGDDLEDLFEEGKMFDGSSVEG
CHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCHHHHCCCHHHHHHCCCCCCCCCCCH
WKAINKADMLLMPIAETAVVDPFARIPTLTLRCSIYDPNTMQSYDRDPRSIAIRAENYLK
HHHCCCCCEEEEECHHHHHHCCHHHCCEEEEEEEEECCCHHHHHCCCCCEEEEEEHHHHH
STGIADSVMFGPEPEFFLFDDVRYNIGMNNVSYKIDDIEAAWNTNRKYEDGNTGYRPLKK
HCCCCCCEEECCCCCEEEEECEEEEECCCCCEEEEECCHHHCCCCCCCCCCCCCCCCCCC
GGYCAVAPIDSAHDIRSEMCLILEEMGLVVEAHHHEVATAGQNEIATKFNSLTLKADETQ
CCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCHHHHHHHCCEEEECCHHH
IYKYVVQNVALEHGKTACFMPKPFAGDNGSGMHCNMSLSKDGKNVFMGDKYAGLSETALY
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCHHHHH
YIGGIIKHAKALNAFTNPSTNSYKRLVPGFEAPVLLAYSASNRSASIRIPAVTSPKATRV
HHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCEE
EARFPDPLANPYLCFAALLMAGLDGVVNKIHPGDAMDKNLYDLPPEELKEIPAVATSLEE
ECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ALDSLAADYEFLTQGGVFTKEFVEAYINIRRKEVERLNMTPHPVEFEMYYA
HHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]