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The map label for this gene is tagI [H]

Identifier: 126209391

GI number: 126209391

Start: 2167581

End: 2168144

Strand: Direct

Name: tagI [H]

Synonym: APL_1931

Alternate gene names: 126209391

Gene position: 2167581-2168144 (Clockwise)

Preceding gene: 126209390

Following gene: 126209396

Centisome position: 95.3

GC content: 42.38

Gene sequence:

>564_bases
ATGGTAAAGCGTTGTAGTTGGGCGGGAGAGAGTGAAATTTATATCAATTATCACGATCAAGAATGGGGTAAACCTGAATT
TGACAGTCGCAAGCTGTTTGAAAAAATTTGTTTGGAAGGGCAGCAAGCAGGGCTTTCTTGGATTACCGTATTGAAAAAGC
GCGAGGCTTATCGCCAAGCCTTTTATCAATTTGAACCGGAACGTATCGCACAAATGACGGAATCGGATATTGATCAACTT
ATGCAAAATACCGGTTTGATTCGCCATCGAGCCAAATTGGAAGCGATTGTGAAAAATGCTAAGGCATATCTTGCCATGCA
AGCAAACGGAGAAGATTTTAGCCAATTTATTTGGGCATTTGTCGGCGGTAAGCCGCAAATTAACGACGTGCCGGATTTGT
CCGTCGTACCGGCAAAAACTGCCGTTTCGAGCGCAATGTCAAAGGCATTGAAAAAGAAAGGTTTTGTGTTTGTCGGCGAA
ACTACTTGTTATGCGTTTATGCAATCAATGGGGCTTGTTGATGATCATCTGAATGATTGTTGTTGCAAACGTGCGAAAAA
ATGA

Upstream 100 bases:

>100_bases
CCAAGAAAATAGTTGTAACGTTTAGTTAAAAGCTGACCGCTTGTGAGTATGATTTTTATCACAAGCGGTCTTTTTTCATC
AGTTTTTGGAGAATATGGCT

Downstream 100 bases:

>100_bases
TAAAAGGATCAGCCTACAAAAAGAGGAATTTGTAGGCTGATTTTTTATTGATTAATGATCATTAAATAAATGTTTAATGA
CGGCAACCACCAAATTACAT

Product: 3-methyladenine-DNA glycosidase

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase; TAG [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MVKRCSWAGESEIYINYHDQEWGKPEFDSRKLFEKICLEGQQAGLSWITVLKKREAYRQAFYQFEPERIAQMTESDIDQL
MQNTGLIRHRAKLEAIVKNAKAYLAMQANGEDFSQFIWAFVGGKPQINDVPDLSVVPAKTAVSSAMSKALKKKGFVFVGE
TTCYAFMQSMGLVDDHLNDCCCKRAKK

Sequences:

>Translated_187_residues
MVKRCSWAGESEIYINYHDQEWGKPEFDSRKLFEKICLEGQQAGLSWITVLKKREAYRQAFYQFEPERIAQMTESDIDQL
MQNTGLIRHRAKLEAIVKNAKAYLAMQANGEDFSQFIWAFVGGKPQINDVPDLSVVPAKTAVSSAMSKALKKKGFVFVGE
TTCYAFMQSMGLVDDHLNDCCCKRAKK
>Mature_187_residues
MVKRCSWAGESEIYINYHDQEWGKPEFDSRKLFEKICLEGQQAGLSWITVLKKREAYRQAFYQFEPERIAQMTESDIDQL
MQNTGLIRHRAKLEAIVKNAKAYLAMQANGEDFSQFIWAFVGGKPQINDVPDLSVVPAKTAVSSAMSKALKKKGFVFVGE
TTCYAFMQSMGLVDDHLNDCCCKRAKK

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=186, Percent_Identity=57.5268817204301, Blast_Score=246, Evalue=6e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 21308; Mature: 21308

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKRCSWAGESEIYINYHDQEWGKPEFDSRKLFEKICLEGQQAGLSWITVLKKREAYRQA
CCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
FYQFEPERIAQMTESDIDQLMQNTGLIRHRAKLEAIVKNAKAYLAMQANGEDFSQFIWAF
HHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHH
VGGKPQINDVPDLSVVPAKTAVSSAMSKALKKKGFVFVGETTCYAFMQSMGLVDDHLNDC
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCHHHHHHHH
CCKRAKK
HHHHCCC
>Mature Secondary Structure
MVKRCSWAGESEIYINYHDQEWGKPEFDSRKLFEKICLEGQQAGLSWITVLKKREAYRQA
CCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
FYQFEPERIAQMTESDIDQLMQNTGLIRHRAKLEAIVKNAKAYLAMQANGEDFSQFIWAF
HHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHH
VGGKPQINDVPDLSVVPAKTAVSSAMSKALKKKGFVFVGETTCYAFMQSMGLVDDHLNDC
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCHHHHHHHH
CCKRAKK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]