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The map label for this gene is pgaB [H]

Identifier: 126209382

GI number: 126209382

Start: 2158538

End: 2160310

Strand: Direct

Name: pgaB [H]

Synonym: APL_1922

Alternate gene names: 126209382

Gene position: 2158538-2160310 (Clockwise)

Preceding gene: 126209381

Following gene: 126209383

Centisome position: 94.9

GC content: 39.03

Gene sequence:

>1773_bases
ATGATATTTAAAAAATTTAGTCGAATAATTATCGCTTGTTTGTCTTTTTTATCAATAAACGTATTGGCGAATAATACTTA
TAGCGTATTGGCTTACCATTCCGTTGTCGATGAAAGTGCGCCCAAAGATAAAAGATTATATGTCTCGCAGACGATTTCTT
CCGATCAATTAATCGCGCATTTTAATTGGTTTAAAAGTCAAGGATATAACATTGTAAGCTGGCAACAAATTGTTGATGCT
GAAAAGGGGAAAACCAAACTTCCGAATAAAGCGGTATTACTGTCTTTCGATGACGGTTATGAAACGATGTATAGCGTTAT
TTATCCGCTATTAAAGGCTTATAACTATCCGGCGGTCTTTGCGCCCGTTTCAAGTTGGATTAGCACGCCGATGGGACAAA
AAATCCAATACGGTAATGAAAAACTGGATCGCGAGAAATTCTTTGCGACTTGGCAACAAATCGATGAAATGCAAAACAGC
GGTTTAGTTGAAATTGCCTCGCACACTCATGATTTACACCACGGTATAAAAGCAAATCCCGGCGGTAGCCAGCTGGCGGC
GATGATTGCGCCGGAATACAAAAACGGTAAATACGAAACGGAAGAACAATATAAATCCCGTATTCTGAATGATATGAAAA
TATCTTCCGGACTTATTAAGAAACACACGGGCAAAGCGCCGCAAATTATGGTTTGGCCTTATGGCGCTTTTACCGAAACA
ACAATTAAATTGGCAAAAGAAGCCGGAATGCCGTATCACTTTACTTTGAAAGAAAAGGTGAATCGTGTCGGCGATGCGCA
TGTAGGACGTTTTCTCGTTGATGCGGAATCTAATTTCTCCGTAATGGCACGTTATTTGAATCGAGTGGCGGATACGGATA
ACGAATCCTTTGTACAGAGAAAATTACATATTAATTTAGACTTCGTATATAGTCCGGATCCGATTAAGTTTAAGGCGAAT
TACGATGCCTTGATTAGTAATGTCGCTAAATACGGCGTAACGGCGGTGTACTTAAAAGCGTATTCGGATAGCAATAAAGA
CGGCATCATTGATGCGGTCTATTTCCCTAATAAATATCTTCCGGTAAGAGCGGATATTTTTAGTCAGGTGGCTTGGCAAT
TACGTACGCGTGCCGGGGTGAAAGTTTATACGTGGATGCCGGCTTCGCTCGAGAGCTTACCTGAGAATTTACGTCAAGTG
AATGTGATGCAATCGCTTTACCGAGATTTATCGCTTTATTCAAAAACGGACGGATTATTCTTTGACAGCAAAATCGGCAA
ACATAAATGGATCGATAATTCGAAAGAAACGATGGCGCTGACGAAATTGTGGACGGAAGCGGCGGAACCTTACTTCTTCT
TTAATCGATATACGCAAAGAATTTCCAGAAATATTCACCCGTTAGATACGAATTTTACTCAAAATTTAGTCGAGTTTAGT
AAGAATTACGACAGTATTTTAATTGAGGTGCGCCCTTATTCGTTAGGCGGTGTGACGACTAAGCAGGAAGCTAAAAATTG
GTTACTCGATATCGTTCAGCGAGTGAAAGATTCTAATGTTGAGAAGAAACAGATTTTATTCGATTTCTCGGTAGTGAATC
CCAAAACATCGGAAAATCTCTCGTCGGAAGAGTTGATCAGCTGGATTAAATTATTAGAAAAACATCAAATAATAAGTTTC
GGTTATTACCCGAATAGATATTTATTTGATGAAAAAATGCTGCAAAAAATGAAACCTTATGTTTCAAGTAATAGAGATAT
CACAAGGAAGTAG

Upstream 100 bases:

>100_bases
ATGAAATCGGTCGTAAGAAAAATATTTATGACGGTGCGGCCGAATTTAATAACTACGGTAACGTGAATTTTTCGTATTCT
TTTTAATTTAGGTAATAACA

Downstream 100 bases:

>100_bases
GCGGATATATGATTCTAGAAATATTCAGTTTATTTGTATTTGCTTATCCGGCGGTAATGGCATTTTATTGGGCTTTTGCA
GGGCTTACATATTTTTTATT

Product: biofilm PGA synthesis lipoprotein PgaB precursor

Products: NA

Alternate protein names: PGA N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase [H]

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MIFKKFSRIIIACLSFLSINVLANNTYSVLAYHSVVDESAPKDKRLYVSQTISSDQLIAHFNWFKSQGYNIVSWQQIVDA
EKGKTKLPNKAVLLSFDDGYETMYSVIYPLLKAYNYPAVFAPVSSWISTPMGQKIQYGNEKLDREKFFATWQQIDEMQNS
GLVEIASHTHDLHHGIKANPGGSQLAAMIAPEYKNGKYETEEQYKSRILNDMKISSGLIKKHTGKAPQIMVWPYGAFTET
TIKLAKEAGMPYHFTLKEKVNRVGDAHVGRFLVDAESNFSVMARYLNRVADTDNESFVQRKLHINLDFVYSPDPIKFKAN
YDALISNVAKYGVTAVYLKAYSDSNKDGIIDAVYFPNKYLPVRADIFSQVAWQLRTRAGVKVYTWMPASLESLPENLRQV
NVMQSLYRDLSLYSKTDGLFFDSKIGKHKWIDNSKETMALTKLWTEAAEPYFFFNRYTQRISRNIHPLDTNFTQNLVEFS
KNYDSILIEVRPYSLGGVTTKQEAKNWLLDIVQRVKDSNVEKKQILFDFSVVNPKTSENLSSEELISWIKLLEKHQIISF
GYYPNRYLFDEKMLQKMKPYVSSNRDITRK

Sequences:

>Translated_590_residues
MIFKKFSRIIIACLSFLSINVLANNTYSVLAYHSVVDESAPKDKRLYVSQTISSDQLIAHFNWFKSQGYNIVSWQQIVDA
EKGKTKLPNKAVLLSFDDGYETMYSVIYPLLKAYNYPAVFAPVSSWISTPMGQKIQYGNEKLDREKFFATWQQIDEMQNS
GLVEIASHTHDLHHGIKANPGGSQLAAMIAPEYKNGKYETEEQYKSRILNDMKISSGLIKKHTGKAPQIMVWPYGAFTET
TIKLAKEAGMPYHFTLKEKVNRVGDAHVGRFLVDAESNFSVMARYLNRVADTDNESFVQRKLHINLDFVYSPDPIKFKAN
YDALISNVAKYGVTAVYLKAYSDSNKDGIIDAVYFPNKYLPVRADIFSQVAWQLRTRAGVKVYTWMPASLESLPENLRQV
NVMQSLYRDLSLYSKTDGLFFDSKIGKHKWIDNSKETMALTKLWTEAAEPYFFFNRYTQRISRNIHPLDTNFTQNLVEFS
KNYDSILIEVRPYSLGGVTTKQEAKNWLLDIVQRVKDSNVEKKQILFDFSVVNPKTSENLSSEELISWIKLLEKHQIISF
GYYPNRYLFDEKMLQKMKPYVSSNRDITRK
>Mature_590_residues
MIFKKFSRIIIACLSFLSINVLANNTYSVLAYHSVVDESAPKDKRLYVSQTISSDQLIAHFNWFKSQGYNIVSWQQIVDA
EKGKTKLPNKAVLLSFDDGYETMYSVIYPLLKAYNYPAVFAPVSSWISTPMGQKIQYGNEKLDREKFFATWQQIDEMQNS
GLVEIASHTHDLHHGIKANPGGSQLAAMIAPEYKNGKYETEEQYKSRILNDMKISSGLIKKHTGKAPQIMVWPYGAFTET
TIKLAKEAGMPYHFTLKEKVNRVGDAHVGRFLVDAESNFSVMARYLNRVADTDNESFVQRKLHINLDFVYSPDPIKFKAN
YDALISNVAKYGVTAVYLKAYSDSNKDGIIDAVYFPNKYLPVRADIFSQVAWQLRTRAGVKVYTWMPASLESLPENLRQV
NVMQSLYRDLSLYSKTDGLFFDSKIGKHKWIDNSKETMALTKLWTEAAEPYFFFNRYTQRISRNIHPLDTNFTQNLVEFS
KNYDSILIEVRPYSLGGVTTKQEAKNWLLDIVQRVKDSNVEKKQILFDFSVVNPKTSENLSSEELISWIKLLEKHQIISF
GYYPNRYLFDEKMLQKMKPYVSSNRDITRK

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PGA), a biofilm adhesin polysaccharide. N- deacetylation promotes PGA export through the PgaA porin [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1787260, Length=642, Percent_Identity=30.6853582554517, Blast_Score=311, Evalue=8e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 67985; Mature: 67985

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFKKFSRIIIACLSFLSINVLANNTYSVLAYHSVVDESAPKDKRLYVSQTISSDQLIAH
CCHHHHHHHHHHHHHHHHEEEEECCCEEEEEHHHHHHCCCCCCCEEEEEECCCCCCEEHH
FNWFKSQGYNIVSWQQIVDAEKGKTKLPNKAVLLSFDDGYETMYSVIYPLLKAYNYPAVF
HHHHHHCCCCEEHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEH
APVSSWISTPMGQKIQYGNEKLDREKFFATWQQIDEMQNSGLVEIASHTHDLHHGIKANP
HHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCC
GGSQLAAMIAPEYKNGKYETEEQYKSRILNDMKISSGLIKKHTGKAPQIMVWPYGAFTET
CHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEECCCCHHHH
TIKLAKEAGMPYHFTLKEKVNRVGDAHVGRFLVDAESNFSVMARYLNRVADTDNESFVQR
HHHHHHHCCCCEEEEHHHHHHHHCCHHHCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHE
KLHINLDFVYSPDPIKFKANYDALISNVAKYGVTAVYLKAYSDSNKDGIIDAVYFPNKYL
EEEEEEEEEECCCCEEEECCHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEEECCCCCC
PVRADIFSQVAWQLRTRAGVKVYTWMPASLESLPENLRQVNVMQSLYRDLSLYSKTDGLF
CCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
FDSKIGKHKWIDNSKETMALTKLWTEAAEPYFFFNRYTQRISRNIHPLDTNFTQNLVEFS
EECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
KNYDSILIEVRPYSLGGVTTKQEAKNWLLDIVQRVKDSNVEKKQILFDFSVVNPKTSENL
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCCCCC
SSEELISWIKLLEKHQIISFGYYPNRYLFDEKMLQKMKPYVSSNRDITRK
CHHHHHHHHHHHHHCCEEEECCCCCCEEHHHHHHHHHCHHHCCCCCCCCC
>Mature Secondary Structure
MIFKKFSRIIIACLSFLSINVLANNTYSVLAYHSVVDESAPKDKRLYVSQTISSDQLIAH
CCHHHHHHHHHHHHHHHHEEEEECCCEEEEEHHHHHHCCCCCCCEEEEEECCCCCCEEHH
FNWFKSQGYNIVSWQQIVDAEKGKTKLPNKAVLLSFDDGYETMYSVIYPLLKAYNYPAVF
HHHHHHCCCCEEHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEH
APVSSWISTPMGQKIQYGNEKLDREKFFATWQQIDEMQNSGLVEIASHTHDLHHGIKANP
HHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCC
GGSQLAAMIAPEYKNGKYETEEQYKSRILNDMKISSGLIKKHTGKAPQIMVWPYGAFTET
CHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEECCCCHHHH
TIKLAKEAGMPYHFTLKEKVNRVGDAHVGRFLVDAESNFSVMARYLNRVADTDNESFVQR
HHHHHHHCCCCEEEEHHHHHHHHCCHHHCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHE
KLHINLDFVYSPDPIKFKANYDALISNVAKYGVTAVYLKAYSDSNKDGIIDAVYFPNKYL
EEEEEEEEEECCCCEEEECCHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEEECCCCCC
PVRADIFSQVAWQLRTRAGVKVYTWMPASLESLPENLRQVNVMQSLYRDLSLYSKTDGLF
CCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
FDSKIGKHKWIDNSKETMALTKLWTEAAEPYFFFNRYTQRISRNIHPLDTNFTQNLVEFS
EECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
KNYDSILIEVRPYSLGGVTTKQEAKNWLLDIVQRVKDSNVEKKQILFDFSVVNPKTSENL
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCCCCCC
SSEELISWIKLLEKHQIISFGYYPNRYLFDEKMLQKMKPYVSSNRDITRK
CHHHHHHHHHHHHHCCEEEECCCCCCEEHHHHHHHHHCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]