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The map label for this gene is pgaC [H]

Identifier: 126209383

GI number: 126209383

Start: 2160319

End: 2161554

Strand: Direct

Name: pgaC [H]

Synonym: APL_1923

Alternate gene names: 126209383

Gene position: 2160319-2161554 (Clockwise)

Preceding gene: 126209382

Following gene: 126209384

Centisome position: 94.98

GC content: 41.02

Gene sequence:

>1236_bases
ATGATTCTAGAAATATTCAGTTTATTTGTATTTGCTTATCCGGCGGTAATGGCATTTTATTGGGCTTTTGCAGGGCTTAC
ATATTTTTTATTTAAAGAAAAGTTAAAAGTACCGCCGAATTTTGATCAAATGAAGCATGAAGAAGTACCGTTAGTCAGCT
TAATGGTGCCTTGCTATAACGAATCGGATAACTTAGACGAAGCGATTCCGCATTTGCTCAATCTAAAATATCCGAATTAC
GAACTGATTTTTATTAATGACGGCAGTAAAGATCATACCGGAGAGATTATCGATAAGTGGGCGAAAAGAGATAAGCGCAT
CGTTGCGTTACACCAAGCGAACTCCGGTAAAGCCAGCGCGTTAAATAACGGTCTGCGAATCGCAAGAGGCAAGTATGTCG
GTTGCATAGACGGTGATGCGGTATTGGATTACAAGGCGTTGGATTATATGGTGCAGGCGTTGGAATCTAATCCTCGATAC
GGTGCGGTGACCGGTAATCCGCGAGTACGTAATCGAAGCACGATTTTAGGACGCTTACAAGTGTCCGAATTCAGCTCGAT
TATCGGTTTAATTAAACGTGCGCAATGTTTAATGGGAACCATCTTTACCGTGTCGGGCGTGTGTTGTTTATTCAGAAAAG
ACATAATGTTCGAGATCGGCGGTTGGAGTACGAACATGATTACGGAAGATATTGACGTCAGTTGGAAAATCCAAACGTCG
GGTTATGACATCTTCTACGAACCGAGAGCGCTATGCTGGGTATTGATGCCGGAAACCATAAACGGTCTGTTTAAACAACG
TCTTCGTTGGGCGCAAGGCGGTGCGGAAACCATGATGAAATATTTTCCGCAAATTTGGCGTTTAAAAAATCGCCGTTTGT
GGCCGATGTTTATCGAATATATTGTGACCGCAATTTGGGCGTCGTTACTTTTAGTTTCAATTTTACTGAGTATATATAAC
CTGATCTTTGATAACCAAATCGGTTTGTTGGATTGGGCCGAGTTAAAACCGAGCATTGCGATACTGTTTATCGCTTTTTT
CACTCAATTAAGTATCAGTCTTTATATCGATAATCGCTATGAAAAAGGCGTAGTGAAATATGTATTTTCTTGTATTTGGT
ATCCTTGGCTGTATTGGAGCCTAAATACGATTACTTTACTGTGCGGTATCCCAAAAGCGATTTTCCGAAATAAAACCAAA
CTAGCGGTATGGACCAGTCCTGATAGAGGAGTTTAA

Upstream 100 bases:

>100_bases
GTTATTACCCGAATAGATATTTATTTGATGAAAAAATGCTGCAAAAAATGAAACCTTATGTTTCAAGTAATAGAGATATC
ACAAGGAAGTAGGCGGATAT

Downstream 100 bases:

>100_bases
TATGTCGGTGCAACAATTACAGAAATTAATGATTATTAACAAAACTAAACAACTCGGTTGGTGGATTCGTTTAAAAAGCT
TGTTTATCACTTTGCTGACT

Product: N-glycosyltransferase

Products: NA

Alternate protein names: PGA synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm PGA synthesis protein PgaC; N-acetylglucosaminyltransferase PgaC [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MILEIFSLFVFAYPAVMAFYWAFAGLTYFLFKEKLKVPPNFDQMKHEEVPLVSLMVPCYNESDNLDEAIPHLLNLKYPNY
ELIFINDGSKDHTGEIIDKWAKRDKRIVALHQANSGKASALNNGLRIARGKYVGCIDGDAVLDYKALDYMVQALESNPRY
GAVTGNPRVRNRSTILGRLQVSEFSSIIGLIKRAQCLMGTIFTVSGVCCLFRKDIMFEIGGWSTNMITEDIDVSWKIQTS
GYDIFYEPRALCWVLMPETINGLFKQRLRWAQGGAETMMKYFPQIWRLKNRRLWPMFIEYIVTAIWASLLLVSILLSIYN
LIFDNQIGLLDWAELKPSIAILFIAFFTQLSISLYIDNRYEKGVVKYVFSCIWYPWLYWSLNTITLLCGIPKAIFRNKTK
LAVWTSPDRGV

Sequences:

>Translated_411_residues
MILEIFSLFVFAYPAVMAFYWAFAGLTYFLFKEKLKVPPNFDQMKHEEVPLVSLMVPCYNESDNLDEAIPHLLNLKYPNY
ELIFINDGSKDHTGEIIDKWAKRDKRIVALHQANSGKASALNNGLRIARGKYVGCIDGDAVLDYKALDYMVQALESNPRY
GAVTGNPRVRNRSTILGRLQVSEFSSIIGLIKRAQCLMGTIFTVSGVCCLFRKDIMFEIGGWSTNMITEDIDVSWKIQTS
GYDIFYEPRALCWVLMPETINGLFKQRLRWAQGGAETMMKYFPQIWRLKNRRLWPMFIEYIVTAIWASLLLVSILLSIYN
LIFDNQIGLLDWAELKPSIAILFIAFFTQLSISLYIDNRYEKGVVKYVFSCIWYPWLYWSLNTITLLCGIPKAIFRNKTK
LAVWTSPDRGV
>Mature_411_residues
MILEIFSLFVFAYPAVMAFYWAFAGLTYFLFKEKLKVPPNFDQMKHEEVPLVSLMVPCYNESDNLDEAIPHLLNLKYPNY
ELIFINDGSKDHTGEIIDKWAKRDKRIVALHQANSGKASALNNGLRIARGKYVGCIDGDAVLDYKALDYMVQALESNPRY
GAVTGNPRVRNRSTILGRLQVSEFSSIIGLIKRAQCLMGTIFTVSGVCCLFRKDIMFEIGGWSTNMITEDIDVSWKIQTS
GYDIFYEPRALCWVLMPETINGLFKQRLRWAQGGAETMMKYFPQIWRLKNRRLWPMFIEYIVTAIWASLLLVSILLSIYN
LIFDNQIGLLDWAELKPSIAILFIAFFTQLSISLYIDNRYEKGVVKYVFSCIWYPWLYWSLNTITLLCGIPKAIFRNKTK
LAVWTSPDRGV

Specific function: Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N- acetylglucosamine to produce the linear homopolymer poly-beta-1,6- N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide [H]

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=411, Percent_Identity=44.2822384428224, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1786560, Length=219, Percent_Identity=31.0502283105023, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87082284, Length=254, Percent_Identity=25.9842519685039, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790044, Length=88, Percent_Identity=37.5, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 47370; Mature: 47370

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILEIFSLFVFAYPAVMAFYWAFAGLTYFLFKEKLKVPPNFDQMKHEEVPLVSLMVPCYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEECCC
ESDNLDEAIPHLLNLKYPNYELIFINDGSKDHTGEIIDKWAKRDKRIVALHQANSGKASA
CCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHH
LNNGLRIARGKYVGCIDGDAVLDYKALDYMVQALESNPRYGAVTGNPRVRNRSTILGRLQ
HCCCCEEECCCEEEEECCCCEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHEEEEE
VSEFSSIIGLIKRAQCLMGTIFTVSGVCCLFRKDIMFEIGGWSTNMITEDIDVSWKIQTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEECCCEEEEEEEC
GYDIFYEPRALCWVLMPETINGLFKQRLRWAQGGAETMMKYFPQIWRLKNRRLWPMFIEY
CCEEEECCCEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
IVTAIWASLLLVSILLSIYNLIFDNQIGLLDWAELKPSIAILFIAFFTQLSISLYIDNRY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHEEEEEECCCC
EKGVVKYVFSCIWYPWLYWSLNTITLLCGIPKAIFRNKTKLAVWTSPDRGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure
MILEIFSLFVFAYPAVMAFYWAFAGLTYFLFKEKLKVPPNFDQMKHEEVPLVSLMVPCYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEECCC
ESDNLDEAIPHLLNLKYPNYELIFINDGSKDHTGEIIDKWAKRDKRIVALHQANSGKASA
CCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHH
LNNGLRIARGKYVGCIDGDAVLDYKALDYMVQALESNPRYGAVTGNPRVRNRSTILGRLQ
HCCCCEEECCCEEEEECCCCEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHEEEEE
VSEFSSIIGLIKRAQCLMGTIFTVSGVCCLFRKDIMFEIGGWSTNMITEDIDVSWKIQTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEECCCEEEEEEEC
GYDIFYEPRALCWVLMPETINGLFKQRLRWAQGGAETMMKYFPQIWRLKNRRLWPMFIEY
CCEEEECCCEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
IVTAIWASLLLVSILLSIYNLIFDNQIGLLDWAELKPSIAILFIAFFTQLSISLYIDNRY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHEEEEEECCCC
EKGVVKYVFSCIWYPWLYWSLNTITLLCGIPKAIFRNKTKLAVWTSPDRGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]