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The map label for this gene is pbpB [H]

Identifier: 126209285

GI number: 126209285

Start: 2043868

End: 2046270

Strand: Reverse

Name: pbpB [H]

Synonym: APL_1823

Alternate gene names: 126209285

Gene position: 2046270-2043868 (Counterclockwise)

Preceding gene: 126209286

Following gene: 126209282

Centisome position: 89.97

GC content: 44.78

Gene sequence:

>2403_bases
ATGTCAAACAACCAAACTCAAACACCGGAACAAGACGAAACTCCGTCAAAGAAAAAGCCGTTTTTTATTCCGACCTGCCT
TAAATTAGGGCTTGTCGGCGCTTGCTTTGCCGCATTCTACGGGATTTATCTCGACGGACGTATTCGAGCGAAAATGGACG
GGCAAGTTTGGCAATTACCTGCAGAAGTTTATAGCCGGATTGAAAGTGTCAGCGTTGAAGATAAATTATCGCTTGAAGCG
ATCAAACAAGTGTTATTGGATAACGGTTATCGCCAGGTTTCTCAAATTGCGACGCCGGGCGATTTCAAAATTGAAAACAA
TACGCTTGTTTTATTACGTCGTGCTTTCCCGTTTCCTGAAACACCGGAAGCACAACGTATTTTGCGTTTACGTTTTGACA
ACGACAAACTGACGCATATCGAAGATTTAGTTCAGCGTCGATTAATTAACGAGTTTCGACTTGACCCGAAATTAATTGCA
ATGCTGCATTCGGATAATGACGAAGAACGCCAAGCGTTACGTTTGCAGCAATACCCGTATTTCCTTATTCAAGCGTTAAT
TCTAACCGAAGATAAGCGTTTCTATCAGCACGACGGTATCAGCCCGCTAGGCATTGCGCGAGCCTTAATGGCAAACTATC
AGGCGGGCAGAACGGTACAGGGTGCGAGTACGCTTACTCAACAATTAGTTAAAAACCTGTTTTTAACCAGCGAAAAAACC
ATCGTGCGTAAAGTGAATGAAGCGTTAATGTCGCTCATTTTGGATTTTCGCTACGATAAAAACCGCATTCTTGAAACTTA
CTTAAACGAGATTTATTTAGGTCAAAACGGTAGCTATCAAGTGCACGGATTTGCCTTGGCAAGCCAATTCTATTTCGGTC
GTCCGATTCAAGAAATCACCCCGTCACAAATGGCGTTATTGGTCGGTATGGTGAAAGGACCTTCGCTCTATAATCCGTGG
CGACATCCGGAAGCCGCACTTGAACGTCGTAATGTGGTGTTAAAATTATTACTAGAAAACCAAGCGATTACCCAGCCGGA
GTATGAATTATTGGTAAAACAACCGCTCGGCGTAAAAGAAAAAGGCACGATTTACCGCCAACAACCGGCATTTATGCAAG
CGTTAAATCTTGATCTAAAAGCGGAACTCGGCGAAAGCAAATATGCGCAGCTTTCCGGTGCGAAGATTTTTACGACATTA
GACAGAAAGCAGCAGCGTTCGGCGGAACAGGCGGTAATTAACGGATTGGAAAAACTGGAAGAGTCGAATAGTAAAATCAG
AGATTTACAATCCGCAATCGTAGTGGCGGAATATAAAACCGGTAAAGTGCGTGCGATTGTCGGCGACCGTTTAACCCAAT
TTGCCGGTTTTAACCGTGCGATTCAGACTAAACGCCAAATCGGTTCATTGGTCAAACCTTCGATTTATGCGATCGCGCTT
TCGGATCCGAAGAATTTCCGTTTAAATACGCCGATCCAAAACCAACCGATCACCATTTATACCAAAGGCTCTCCGCCTTG
GACACCGAAAAACTATGATAAACGCTTTAGCGGTTCGGTCATGTTAATGGATGCGTTAGTGCGCTCGTTAAATATTCCGA
CCGTGAATATCGGTATGAAAGTCGGCTTAAAGAACGTGATTGCGAAGCAAAAAGAGATGGGTTGGGATAAAGCCGATATT
CCGGCGTATCCTTCGATGTTGCTCGGTTCGTACTCTATTTCGCCGTATGATGTGACCAAGTCTTATCAAGTATTGGCAAA
TAACGGTTTAAAAACACCGCTTACGACGATTGAAGCCATTATGTCGCATGACGGTAGCCTGCTTTACCAGCGCAATACCG
AAGAAGTCAGCCGCCAAGTATTACCGCAAGAGGCAACAATTCAAACACTATATGCTATGCAACAAGTGGTAGAACGAGGT
ACGGCTCGCAGCTTACAAAACGAATTCGCTCATTTACGTTTAGCCGGAAAAACCGGGACGACCAATAATGCGCGCGATAC
TTGGTTCGTCGGAGTGGACGGTGAAAACGTCACCACCGTATGGTTAGGGAAAGATAATAATACCGATACCAATTTAACCG
GTTCCAGCGGTGCATTGTTCGTGTATAAACAATATTTGGAACGTGCTTTACCGACCGCGTTTAAATTGCCAAAAGCGAAA
AGTATGCAATGGGTGGGCATCAATGGCTACGGTACGATTAATTGCGATCCGAACCGTCAAATTCCAATGTGGCGAGATCG
GGGACAGCACTACTGTACTAATGCCGGTAATGTGGTCGAAGCCGCCAAACCGACCGTATTGGAAGCGATTTCATTAGATA
AAACCAAAACGGAAAATACCAAAGCCGATGTGGCTGCGGATCAACAAGTCTTACCGGCGGAAGAAATCGCACCGACCGAA
TAA

Upstream 100 bases:

>100_bases
TAAATTTAAACGTAATCCGTAATGCCTTCTATTACCTCCCCCTATAACGGGGAGGTGCTGACAAGCGGTCCGATTCGCTG
ACTTTTTTGCAAATAATTTT

Downstream 100 bases:

>100_bases
GCAAGCGGTCGAAAAACGTGAAATTTTTACGAAACGCGGATCAAAAAACGCATATTCAATGATGGTTGAATATGCGTTTT
TATTTAAGTGAATCTCAAAT

Product: penicillin-binding protein 1B (PBP1b)

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 800; Mature: 799

Protein sequence:

>800_residues
MSNNQTQTPEQDETPSKKKPFFIPTCLKLGLVGACFAAFYGIYLDGRIRAKMDGQVWQLPAEVYSRIESVSVEDKLSLEA
IKQVLLDNGYRQVSQIATPGDFKIENNTLVLLRRAFPFPETPEAQRILRLRFDNDKLTHIEDLVQRRLINEFRLDPKLIA
MLHSDNDEERQALRLQQYPYFLIQALILTEDKRFYQHDGISPLGIARALMANYQAGRTVQGASTLTQQLVKNLFLTSEKT
IVRKVNEALMSLILDFRYDKNRILETYLNEIYLGQNGSYQVHGFALASQFYFGRPIQEITPSQMALLVGMVKGPSLYNPW
RHPEAALERRNVVLKLLLENQAITQPEYELLVKQPLGVKEKGTIYRQQPAFMQALNLDLKAELGESKYAQLSGAKIFTTL
DRKQQRSAEQAVINGLEKLEESNSKIRDLQSAIVVAEYKTGKVRAIVGDRLTQFAGFNRAIQTKRQIGSLVKPSIYAIAL
SDPKNFRLNTPIQNQPITIYTKGSPPWTPKNYDKRFSGSVMLMDALVRSLNIPTVNIGMKVGLKNVIAKQKEMGWDKADI
PAYPSMLLGSYSISPYDVTKSYQVLANNGLKTPLTTIEAIMSHDGSLLYQRNTEEVSRQVLPQEATIQTLYAMQQVVERG
TARSLQNEFAHLRLAGKTGTTNNARDTWFVGVDGENVTTVWLGKDNNTDTNLTGSSGALFVYKQYLERALPTAFKLPKAK
SMQWVGINGYGTINCDPNRQIPMWRDRGQHYCTNAGNVVEAAKPTVLEAISLDKTKTENTKADVAADQQVLPAEEIAPTE

Sequences:

>Translated_800_residues
MSNNQTQTPEQDETPSKKKPFFIPTCLKLGLVGACFAAFYGIYLDGRIRAKMDGQVWQLPAEVYSRIESVSVEDKLSLEA
IKQVLLDNGYRQVSQIATPGDFKIENNTLVLLRRAFPFPETPEAQRILRLRFDNDKLTHIEDLVQRRLINEFRLDPKLIA
MLHSDNDEERQALRLQQYPYFLIQALILTEDKRFYQHDGISPLGIARALMANYQAGRTVQGASTLTQQLVKNLFLTSEKT
IVRKVNEALMSLILDFRYDKNRILETYLNEIYLGQNGSYQVHGFALASQFYFGRPIQEITPSQMALLVGMVKGPSLYNPW
RHPEAALERRNVVLKLLLENQAITQPEYELLVKQPLGVKEKGTIYRQQPAFMQALNLDLKAELGESKYAQLSGAKIFTTL
DRKQQRSAEQAVINGLEKLEESNSKIRDLQSAIVVAEYKTGKVRAIVGDRLTQFAGFNRAIQTKRQIGSLVKPSIYAIAL
SDPKNFRLNTPIQNQPITIYTKGSPPWTPKNYDKRFSGSVMLMDALVRSLNIPTVNIGMKVGLKNVIAKQKEMGWDKADI
PAYPSMLLGSYSISPYDVTKSYQVLANNGLKTPLTTIEAIMSHDGSLLYQRNTEEVSRQVLPQEATIQTLYAMQQVVERG
TARSLQNEFAHLRLAGKTGTTNNARDTWFVGVDGENVTTVWLGKDNNTDTNLTGSSGALFVYKQYLERALPTAFKLPKAK
SMQWVGINGYGTINCDPNRQIPMWRDRGQHYCTNAGNVVEAAKPTVLEAISLDKTKTENTKADVAADQQVLPAEEIAPTE
>Mature_799_residues
SNNQTQTPEQDETPSKKKPFFIPTCLKLGLVGACFAAFYGIYLDGRIRAKMDGQVWQLPAEVYSRIESVSVEDKLSLEAI
KQVLLDNGYRQVSQIATPGDFKIENNTLVLLRRAFPFPETPEAQRILRLRFDNDKLTHIEDLVQRRLINEFRLDPKLIAM
LHSDNDEERQALRLQQYPYFLIQALILTEDKRFYQHDGISPLGIARALMANYQAGRTVQGASTLTQQLVKNLFLTSEKTI
VRKVNEALMSLILDFRYDKNRILETYLNEIYLGQNGSYQVHGFALASQFYFGRPIQEITPSQMALLVGMVKGPSLYNPWR
HPEAALERRNVVLKLLLENQAITQPEYELLVKQPLGVKEKGTIYRQQPAFMQALNLDLKAELGESKYAQLSGAKIFTTLD
RKQQRSAEQAVINGLEKLEESNSKIRDLQSAIVVAEYKTGKVRAIVGDRLTQFAGFNRAIQTKRQIGSLVKPSIYAIALS
DPKNFRLNTPIQNQPITIYTKGSPPWTPKNYDKRFSGSVMLMDALVRSLNIPTVNIGMKVGLKNVIAKQKEMGWDKADIP
AYPSMLLGSYSISPYDVTKSYQVLANNGLKTPLTTIEAIMSHDGSLLYQRNTEEVSRQVLPQEATIQTLYAMQQVVERGT
ARSLQNEFAHLRLAGKTGTTNNARDTWFVGVDGENVTTVWLGKDNNTDTNLTGSSGALFVYKQYLERALPTAFKLPKAKS
MQWVGINGYGTINCDPNRQIPMWRDRGQHYCTNAGNVVEAAKPTVLEAISLDKTKTENTKADVAADQQVLPAEEIAPTE

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=730, Percent_Identity=47.2602739726027, Blast_Score=675, Evalue=0.0,
Organism=Escherichia coli, GI87082258, Length=290, Percent_Identity=35.1724137931034, Blast_Score=175, Evalue=1e-44,
Organism=Escherichia coli, GI1788867, Length=519, Percent_Identity=28.5163776493256, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1789601, Length=130, Percent_Identity=37.6923076923077, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 90073; Mature: 89942

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNQTQTPEQDETPSKKKPFFIPTCLKLGLVGACFAAFYGIYLDGRIRAKMDGQVWQLP
CCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHEECCEEEEEECCCEEECH
AEVYSRIESVSVEDKLSLEAIKQVLLDNGYRQVSQIATPGDFKIENNTLVLLRRAFPFPE
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEECCCEEEEEEECCCCCC
TPEAQRILRLRFDNDKLTHIEDLVQRRLINEFRLDPKLIAMLHSDNDEERQALRLQQYPY
CCCHHHEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHEEEEECCCHHHHHHHHHHHCHH
FLIQALILTEDKRFYQHDGISPLGIARALMANYQAGRTVQGASTLTQQLVKNLFLTSEKT
HHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHCCHHH
IVRKVNEALMSLILDFRYDKNRILETYLNEIYLGQNGSYQVHGFALASQFYFGRPIQEIT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCEEEEEEEEEHHHHCCCCHHHCC
PSQMALLVGMVKGPSLYNPWRHPEAALERRNVVLKLLLENQAITQPEYELLVKQPLGVKE
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCHHHHHCCCCCCCCC
KGTIYRQQPAFMQALNLDLKAELGESKYAQLSGAKIFTTLDRKQQRSAEQAVINGLEKLE
CCCEEECCHHHHHHHCCCCEECCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
ESNSKIRDLQSAIVVAEYKTGKVRAIVGDRLTQFAGFNRAIQTKRQIGSLVKPSIYAIAL
HCCHHHHHHHHHEEEEEECCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEE
SDPKNFRLNTPIQNQPITIYTKGSPPWTPKNYDKRFSGSVMLMDALVRSLNIPTVNIGMK
CCCCCCEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECHH
VGLKNVIAKQKEMGWDKADIPAYPSMLLGSYSISPYDVTKSYQVLANNGLKTPLTTIEAI
HHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHH
MSHDGSLLYQRNTEEVSRQVLPQEATIQTLYAMQQVVERGTARSLQNEFAHLRLAGKTGT
HCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
TNNARDTWFVGVDGENVTTVWLGKDNNTDTNLTGSSGALFVYKQYLERALPTAFKLPKAK
CCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCC
SMQWVGINGYGTINCDPNRQIPMWRDRGQHYCTNAGNVVEAAKPTVLEAISLDKTKTENT
CEEEEEECCEEEEECCCCCCCCCHHHCCCEEECCCCCEECCCCCHHHHHHHCCCCCCCCC
KADVAADQQVLPAEEIAPTE
CHHHCCCCCCCCHHHCCCCC
>Mature Secondary Structure 
SNNQTQTPEQDETPSKKKPFFIPTCLKLGLVGACFAAFYGIYLDGRIRAKMDGQVWQLP
CCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHEECCEEEEEECCCEEECH
AEVYSRIESVSVEDKLSLEAIKQVLLDNGYRQVSQIATPGDFKIENNTLVLLRRAFPFPE
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEECCCEEEEEEECCCCCC
TPEAQRILRLRFDNDKLTHIEDLVQRRLINEFRLDPKLIAMLHSDNDEERQALRLQQYPY
CCCHHHEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHEEEEECCCHHHHHHHHHHHCHH
FLIQALILTEDKRFYQHDGISPLGIARALMANYQAGRTVQGASTLTQQLVKNLFLTSEKT
HHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHCCHHH
IVRKVNEALMSLILDFRYDKNRILETYLNEIYLGQNGSYQVHGFALASQFYFGRPIQEIT
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCCCEEEEEEEEEHHHHCCCCHHHCC
PSQMALLVGMVKGPSLYNPWRHPEAALERRNVVLKLLLENQAITQPEYELLVKQPLGVKE
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCHHHHHCCCCCCCCC
KGTIYRQQPAFMQALNLDLKAELGESKYAQLSGAKIFTTLDRKQQRSAEQAVINGLEKLE
CCCEEECCHHHHHHHCCCCEECCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
ESNSKIRDLQSAIVVAEYKTGKVRAIVGDRLTQFAGFNRAIQTKRQIGSLVKPSIYAIAL
HCCHHHHHHHHHEEEEEECCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEE
SDPKNFRLNTPIQNQPITIYTKGSPPWTPKNYDKRFSGSVMLMDALVRSLNIPTVNIGMK
CCCCCCEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECHH
VGLKNVIAKQKEMGWDKADIPAYPSMLLGSYSISPYDVTKSYQVLANNGLKTPLTTIEAI
HHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHH
MSHDGSLLYQRNTEEVSRQVLPQEATIQTLYAMQQVVERGTARSLQNEFAHLRLAGKTGT
HCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
TNNARDTWFVGVDGENVTTVWLGKDNNTDTNLTGSSGALFVYKQYLERALPTAFKLPKAK
CCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCC
SMQWVGINGYGTINCDPNRQIPMWRDRGQHYCTNAGNVVEAAKPTVLEAISLDKTKTENT
CEEEEEECCEEEEECCCCCCCCCHHHCCCEEECCCCCEECCCCCHHHHHHHCCCCCCCCC
KADVAADQQVLPAEEIAPTE
CHHHCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]