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The map label for this gene is trmD

Identifier: 126209252

GI number: 126209252

Start: 2011631

End: 2012386

Strand: Direct

Name: trmD

Synonym: APL_1788

Alternate gene names: 126209252

Gene position: 2011631-2012386 (Clockwise)

Preceding gene: 126209251

Following gene: 126209253

Centisome position: 88.44

GC content: 45.63

Gene sequence:

>756_bases
ATGTGGATTGGTATCATTTCACTGTTCCCCGAAATGTTTAAAGCAATTACCGATTTTGGGGTGACAGGACGTGCGGTAAA
ACAAGATCTTCTGCAAATTCAATGCTGGAATCCTCGCGATTTCACGTTTGATAAACATAAAACGGTTGATGATCGCCCTT
ACGGCGGCGGACCGGGAATGTTAATGATGGTGCAGCCTTTGCGTGATGCGATTCATGCAGCAAAGCAGGCGGCAAAAGCG
GAAGACGGTGTAGAGGCAAAGGTTATTTATCTCTCACCGCAAGGGCGTAAGCTGGATCAACAAGGTGTGAAGACACTGGC
TTCAAATCAGAAACTGATTTTAATTTGTGGACGATACGAAGGGGTTGATGAACGATTGATTCAAACGGAAGTTGATGAAG
AATGGTCGATCGGTGATTATGTGCTTACAGGGGGCGAATTGCCTGCAATGACGTTGATTGACGCCGTTGCGAGATTTGTG
CCCGGAGTGTTAGGTAAACAGGCCTCTGCAGATGAAGATTCATTTGCGACAGGTTTATTGGATTGTCCGCATTACACTCG
CCCGGAAATGTTAGACGGTATTCCTGTTCCCGAAGTGCTGATGTCGGGTCATCACGAAAATATTCGTAAATGGCGATTAG
AACAATCGCTTGAACGTACGTGGTTAAGACGCCCTGAGCTATTGGATAGCCTAGCTCTGACTGACGAACAACGCGTGTTG
TTGGCTAAAATTAAGAAACAACACAAAATCAGTTAA

Upstream 100 bases:

>100_bases
CGCCACCAAGACAATTACGGTGGATTGGGACGCTGGTTTCTAACCTCAGGTATGCGGTGCAGCTTTAACTTTAAGTAGTA
AGAACATTTGAGGATAAATT

Downstream 100 bases:

>100_bases
TTCTAGGATTTAGAAGGTTTTTAAAATGAGTAACATCATCAAACAAATCGAACAAGAACAGTTAAAACAAAACGTACCTA
GCTTCCGTCCGGGTGACACA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MWIGIISLFPEMFKAITDFGVTGRAVKQDLLQIQCWNPRDFTFDKHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKQAAKA
EDGVEAKVIYLSPQGRKLDQQGVKTLASNQKLILICGRYEGVDERLIQTEVDEEWSIGDYVLTGGELPAMTLIDAVARFV
PGVLGKQASADEDSFATGLLDCPHYTRPEMLDGIPVPEVLMSGHHENIRKWRLEQSLERTWLRRPELLDSLALTDEQRVL
LAKIKKQHKIS

Sequences:

>Translated_251_residues
MWIGIISLFPEMFKAITDFGVTGRAVKQDLLQIQCWNPRDFTFDKHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKQAAKA
EDGVEAKVIYLSPQGRKLDQQGVKTLASNQKLILICGRYEGVDERLIQTEVDEEWSIGDYVLTGGELPAMTLIDAVARFV
PGVLGKQASADEDSFATGLLDCPHYTRPEMLDGIPVPEVLMSGHHENIRKWRLEQSLERTWLRRPELLDSLALTDEQRVL
LAKIKKQHKIS
>Mature_251_residues
MWIGIISLFPEMFKAITDFGVTGRAVKQDLLQIQCWNPRDFTFDKHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKQAAKA
EDGVEAKVIYLSPQGRKLDQQGVKTLASNQKLILICGRYEGVDERLIQTEVDEEWSIGDYVLTGGELPAMTLIDAVARFV
PGVLGKQASADEDSFATGLLDCPHYTRPEMLDGIPVPEVLMSGHHENIRKWRLEQSLERTWLRRPELLDSLALTDEQRVL
LAKIKKQHKIS

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=248, Percent_Identity=77.8225806451613, Blast_Score=387, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_ACTP2 (A3N386)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054477.1
- ProteinModelPortal:   A3N386
- SMR:   A3N386
- STRING:   A3N386
- GeneID:   4848951
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1788
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   A3N386
- ProtClustDB:   PRK00026
- BioCyc:   APLE416269:APL_1788-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28221; Mature: 28221

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: BINDING 117-117

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWIGIISLFPEMFKAITDFGVTGRAVKQDLLQIQCWNPRDFTFDKHKTVDDRPYGGGPGM
CEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCC
LMMVQPLRDAIHAAKQAAKAEDGVEAKVIYLSPQGRKLDQQGVKTLASNQKLILICGRYE
EEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCC
GVDERLIQTEVDEEWSIGDYVLTGGELPAMTLIDAVARFVPGVLGKQASADEDSFATGLL
CHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCC
DCPHYTRPEMLDGIPVPEVLMSGHHENIRKWRLEQSLERTWLRRPELLDSLALTDEQRVL
CCCCCCCHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHH
LAKIKKQHKIS
HHHHHHHHCCC
>Mature Secondary Structure
MWIGIISLFPEMFKAITDFGVTGRAVKQDLLQIQCWNPRDFTFDKHKTVDDRPYGGGPGM
CEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCC
LMMVQPLRDAIHAAKQAAKAEDGVEAKVIYLSPQGRKLDQQGVKTLASNQKLILICGRYE
EEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCC
GVDERLIQTEVDEEWSIGDYVLTGGELPAMTLIDAVARFVPGVLGKQASADEDSFATGLL
CHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCC
DCPHYTRPEMLDGIPVPEVLMSGHHENIRKWRLEQSLERTWLRRPELLDSLALTDEQRVL
CCCCCCCHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHH
LAKIKKQHKIS
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA