Click here to switch to the map view.

The map label for this gene is nagB

Identifier: 126209219

GI number: 126209219

Start: 1992011

End: 1992814

Strand: Direct

Name: nagB

Synonym: APL_1755

Alternate gene names: 126209219

Gene position: 1992011-1992814 (Clockwise)

Preceding gene: 126209218

Following gene: 126209220

Centisome position: 87.58

GC content: 44.65

Gene sequence:

>804_bases
ATGCGTCTTATTCCGTTACAAACCAGTGAGCAAGTAAGCCGTTGGGCAGCTCGCCATATTGTTGAACGAATCAATCGATT
TCAGCCGACCGCTGATCGTCCTTTTGTGTTAGGTTTGCCGACCGGCGGTACTCCGTTACAAACCTATAAAGAATTGATTC
GCTTATATCAAGCCGGCGAAGTGAGCTTTCAGCACGTAGTGACCTTTAATATGGACGAATATGTCGGCTTACCTAAGGAA
CATCCGCAGAGTTACCATACCTTTATGTACCGTAACTTCTTTGATCATATCGATATCCAACCGCAAAATATCAATATTTT
GAACGGCAATACCGAGGATCACGATGCGGAATGTCGCCGTTATGAAGAGAAAATCAAGTCATACGGCAAAATTCACTTAT
TTATGGGCGGCGTCGGTGTGGACGGTCATATTGCGTTTAACGAACCGGCTTCATCTTTGGGTTCTCGTACTCGTATTAAA
ACCTTGACCGAAGATACCTTAATCGCCAATTCGCGCTTTTTTGATAATGATATTACTAAAGTGCCGAAATACGCTTTAAC
CGTCGGTGTGGCGACATTACTCGATGCGGAAGAAGTGATGTTGCTGATTACCGGCTATAATAAAGCGTTGGCGTTACAGG
CTTGTGTCGAAGGCTCGGTAAACCATATGTGGACGGTTAGTGCCTTACAACTGCACAAACGAGGCATCGTAGTGTGTGAC
GAACCGGCAACGCAAGAACTCAAAGTCAAAACCGTGAAATATTTCACCCAATTGGAAACGCAAGCGATTCAAAGCGTGTT
GTAA

Upstream 100 bases:

>100_bases
ACCGACAAACAACTTGAAGTGGCAAAAGCGCTTTATGCAAAATTTTTATAAAATTTGACCGCTTGTGATATAGCTATAAC
AACAATAATTAGGAAATCTT

Downstream 100 bases:

>100_bases
GTTTTAAGGTCTGTTACACTATAAATAGACCTTTTCATTTGGAGAACAAGAATGAAATATGCTTTTACTAATAGCGTGAT
TTATACCGCCAAAGAGGTGC

Product: glucosamine-6-phosphate deaminase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MRLIPLQTSEQVSRWAARHIVERINRFQPTADRPFVLGLPTGGTPLQTYKELIRLYQAGEVSFQHVVTFNMDEYVGLPKE
HPQSYHTFMYRNFFDHIDIQPQNINILNGNTEDHDAECRRYEEKIKSYGKIHLFMGGVGVDGHIAFNEPASSLGSRTRIK
TLTEDTLIANSRFFDNDITKVPKYALTVGVATLLDAEEVMLLITGYNKALALQACVEGSVNHMWTVSALQLHKRGIVVCD
EPATQELKVKTVKYFTQLETQAIQSVL

Sequences:

>Translated_267_residues
MRLIPLQTSEQVSRWAARHIVERINRFQPTADRPFVLGLPTGGTPLQTYKELIRLYQAGEVSFQHVVTFNMDEYVGLPKE
HPQSYHTFMYRNFFDHIDIQPQNINILNGNTEDHDAECRRYEEKIKSYGKIHLFMGGVGVDGHIAFNEPASSLGSRTRIK
TLTEDTLIANSRFFDNDITKVPKYALTVGVATLLDAEEVMLLITGYNKALALQACVEGSVNHMWTVSALQLHKRGIVVCD
EPATQELKVKTVKYFTQLETQAIQSVL
>Mature_267_residues
MRLIPLQTSEQVSRWAARHIVERINRFQPTADRPFVLGLPTGGTPLQTYKELIRLYQAGEVSFQHVVTFNMDEYVGLPKE
HPQSYHTFMYRNFFDHIDIQPQNINILNGNTEDHDAECRRYEEKIKSYGKIHLFMGGVGVDGHIAFNEPASSLGSRTRIK
TLTEDTLIANSRFFDNDITKVPKYALTVGVATLLDAEEVMLLITGYNKALALQACVEGSVNHMWTVSALQLHKRGIVVCD
EPATQELKVKTVKYFTQLETQAIQSVL

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI13027378, Length=258, Percent_Identity=62.4031007751938, Blast_Score=343, Evalue=7e-95,
Organism=Homo sapiens, GI19923881, Length=258, Percent_Identity=60.8527131782946, Blast_Score=335, Evalue=3e-92,
Organism=Escherichia coli, GI1786893, Length=266, Percent_Identity=75.5639097744361, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1789530, Length=245, Percent_Identity=28.1632653061224, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI48994958, Length=240, Percent_Identity=25.8333333333333, Blast_Score=71, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17554876, Length=258, Percent_Identity=60.4651162790698, Blast_Score=325, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24581960, Length=255, Percent_Identity=63.1372549019608, Blast_Score=357, Evalue=6e-99,
Organism=Drosophila melanogaster, GI19920764, Length=255, Percent_Identity=63.1372549019608, Blast_Score=357, Evalue=6e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_ACTP2 (A3N353)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054444.1
- ProteinModelPortal:   A3N353
- SMR:   A3N353
- STRING:   A3N353
- GeneID:   4848658
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1755
- eggNOG:   COG0363
- HOGENOM:   HBG725991
- OMA:   HLSITMG
- PhylomeDB:   A3N353
- ProtClustDB:   PRK00443
- BioCyc:   APLE416269:APL_1755-MONOMER
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280
- TIGRFAMs:   TIGR00502

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 30298; Mature: 30298

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 72-72 ACT_SITE 141-141 ACT_SITE 143-143 ACT_SITE 148-148

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIPLQTSEQVSRWAARHIVERINRFQPTADRPFVLGLPTGGTPLQTYKELIRLYQAGE
CEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
VSFQHVVTFNMDEYVGLPKEHPQSYHTFMYRNFFDHIDIQPQNINILNGNTEDHDAECRR
CCEEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHH
YEEKIKSYGKIHLFMGGVGVDGHIAFNEPASSLGSRTRIKTLTEDTLIANSRFFDNDITK
HHHHHHCCCEEEEEEECCCCCCEEEECCCHHHHCCCCEEHECCCCCEEECCCCCCCHHHH
VPKYALTVGVATLLDAEEVMLLITGYNKALALQACVEGSVNHMWTVSALQLHKRGIVVCD
CCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEHHHHHHHHCCCEEEC
EPATQELKVKTVKYFTQLETQAIQSVL
CCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLIPLQTSEQVSRWAARHIVERINRFQPTADRPFVLGLPTGGTPLQTYKELIRLYQAGE
CEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
VSFQHVVTFNMDEYVGLPKEHPQSYHTFMYRNFFDHIDIQPQNINILNGNTEDHDAECRR
CCEEEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHH
YEEKIKSYGKIHLFMGGVGVDGHIAFNEPASSLGSRTRIKTLTEDTLIANSRFFDNDITK
HHHHHHCCCEEEEEEECCCCCCEEEECCCHHHHCCCCEEHECCCCCEEECCCCCCCHHHH
VPKYALTVGVATLLDAEEVMLLITGYNKALALQACVEGSVNHMWTVSALQLHKRGIVVCD
CCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEHHHHHHHHCCCEEEC
EPATQELKVKTVKYFTQLETQAIQSVL
CCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA