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The map label for this gene is nanE

Identifier: 126209216

GI number: 126209216

Start: 1989491

End: 1990180

Strand: Direct

Name: nanE

Synonym: APL_1752

Alternate gene names: 126209216

Gene position: 1989491-1990180 (Clockwise)

Preceding gene: 126209215

Following gene: 126209217

Centisome position: 87.47

GC content: 48.99

Gene sequence:

>690_bases
ATGTCAAAGTTATCTCATTCGGAAGTATTGAATACGATTCGAAACGGTTTAATCGCTTCGTGTCAGCCGGTTGATGACGG
CCCGATGGACAAACCGGAGATCGTGGCGGCGATGGCGCAAGCCTCGCTTATCGGCGGTGCGGCAGGATTACGCATTGAAG
GGGTCGATAATTTAAAAGCGACTCGTCCGACGGTGAAAGCGCCGATTATTGCGATTGTAAAACGTGATTTACCGGATAGT
CCGGTACGTATTACGCCGTTTTTACAAGATATTGATGATCTGGCTGCAGCCGGTGCCGATATTATTGCGGTGGACGGTAC
GGATCGTGTTCGACCGGTCACGATTGAAGCGGCGTTGAAACGTATTCATGAGCTAGGTTGTTTGGCAATGGCGGACTGTT
CGACATTAGCAGAGGGTTTATATTGCCAGCAACTCGGTTTTGATATTGTCGGTAGCACAATGTCGGGTTATACCGGCGGA
GAAGTACCGAATGAACCGGATTATCAGTTAGTTAAAGACTTAAAAGCGGCGGGCTGTTTTGTGATGGCGGAAGGGCGTTA
TAACTCACCGCAACTGGCAAAAACCGCGATTGAAATCGGTGCGGATTGTGTTACGGTCGGTTCGGCACTCACTCGCTTGG
AGCATATTGTCGGCTGGTTTGCTGATGAGATAAAAACTGCGAAAGTTTAA

Upstream 100 bases:

>100_bases
GGGTTACATTTTAATTTACAAGGTTATGCAGTTTTGCGTCAGTTATTAGAAAGCTATGTATAAGCTTTATTTGAAAGCAT
AACGAACTCAAGGAGTTTAT

Downstream 100 bases:

>100_bases
GGGGAATGTATGCGTTGTTTAGCATTAGATATCGGCGGTACTAAGATTGCCTCGGCATTAGTCGAAAACGGAGTGATTTC
CCAACGCCGACAAATCGGCA

Product: N-acetylmannosamine-6-phosphate 2-epimerase

Products: NA

Alternate protein names: ManNAc-6-P epimerase

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSKLSHSEVLNTIRNGLIASCQPVDDGPMDKPEIVAAMAQASLIGGAAGLRIEGVDNLKATRPTVKAPIIAIVKRDLPDS
PVRITPFLQDIDDLAAAGADIIAVDGTDRVRPVTIEAALKRIHELGCLAMADCSTLAEGLYCQQLGFDIVGSTMSGYTGG
EVPNEPDYQLVKDLKAAGCFVMAEGRYNSPQLAKTAIEIGADCVTVGSALTRLEHIVGWFADEIKTAKV

Sequences:

>Translated_229_residues
MSKLSHSEVLNTIRNGLIASCQPVDDGPMDKPEIVAAMAQASLIGGAAGLRIEGVDNLKATRPTVKAPIIAIVKRDLPDS
PVRITPFLQDIDDLAAAGADIIAVDGTDRVRPVTIEAALKRIHELGCLAMADCSTLAEGLYCQQLGFDIVGSTMSGYTGG
EVPNEPDYQLVKDLKAAGCFVMAEGRYNSPQLAKTAIEIGADCVTVGSALTRLEHIVGWFADEIKTAKV
>Mature_228_residues
SKLSHSEVLNTIRNGLIASCQPVDDGPMDKPEIVAAMAQASLIGGAAGLRIEGVDNLKATRPTVKAPIIAIVKRDLPDSP
VRITPFLQDIDDLAAAGADIIAVDGTDRVRPVTIEAALKRIHELGCLAMADCSTLAEGLYCQQLGFDIVGSTMSGYTGGE
VPNEPDYQLVKDLKAAGCFVMAEGRYNSPQLAKTAIEIGADCVTVGSALTRLEHIVGWFADEIKTAKV

Specific function: Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P) (Potential)

COG id: COG3010

COG function: function code G; Putative N-acetylmannosamine-6-phosphate epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nanE family

Homologues:

Organism=Escherichia coli, GI1789617, Length=227, Percent_Identity=60.352422907489, Blast_Score=257, Evalue=5e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NANE_ACTP2 (A3N350)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054441.1
- ProteinModelPortal:   A3N350
- SMR:   A3N350
- STRING:   A3N350
- GeneID:   4849607
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1752
- eggNOG:   COG3010
- HOGENOM:   HBG296941
- OMA:   EHIVSWF
- PhylomeDB:   A3N350
- ProtClustDB:   PRK01130
- BioCyc:   APLE416269:APL_1752-MONOMER
- HAMAP:   MF_01235
- InterPro:   IPR013785
- InterPro:   IPR007260
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF04131 NanE; SSF51366 RibP_bind_barrel

EC number: =5.1.3.9

Molecular weight: Translated: 24161; Mature: 24030

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLSHSEVLNTIRNGLIASCQPVDDGPMDKPEIVAAMAQASLIGGAAGLRIEGVDNLKA
CCCCCHHHHHHHHHHCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCC
TRPTVKAPIIAIVKRDLPDSPVRITPFLQDIDDLAAAGADIIAVDGTDRVRPVTIEAALK
CCCCHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHCCCCEEEECCCCCCEEEEHHHHHH
RIHELGCLAMADCSTLAEGLYCQQLGFDIVGSTMSGYTGGEVPNEPDYQLVKDLKAAGCF
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCEE
VMAEGRYNSPQLAKTAIEIGADCVTVGSALTRLEHIVGWFADEIKTAKV
EEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKLSHSEVLNTIRNGLIASCQPVDDGPMDKPEIVAAMAQASLIGGAAGLRIEGVDNLKA
CCCCHHHHHHHHHHCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCC
TRPTVKAPIIAIVKRDLPDSPVRITPFLQDIDDLAAAGADIIAVDGTDRVRPVTIEAALK
CCCCHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHCCCCEEEECCCCCCEEEEHHHHHH
RIHELGCLAMADCSTLAEGLYCQQLGFDIVGSTMSGYTGGEVPNEPDYQLVKDLKAAGCF
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCEE
VMAEGRYNSPQLAKTAIEIGADCVTVGSALTRLEHIVGWFADEIKTAKV
EEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA