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The map label for this gene is yfkN [H]

Identifier: 126209207

GI number: 126209207

Start: 1979499

End: 1981163

Strand: Reverse

Name: yfkN [H]

Synonym: APL_1743

Alternate gene names: 126209207

Gene position: 1981163-1979499 (Counterclockwise)

Preceding gene: 126209208

Following gene: 126209202

Centisome position: 87.1

GC content: 40.78

Gene sequence:

>1665_bases
ATGAAAAAAGTACTCGGTACTTTATTGGTATTATCTGCAGCGAGCGTTGCAGTTGCGAAAGAAGTGAATATTAAATTTTT
AGGCACGTCGGATATTCACGGGCGTATCGTACCTTGGAACTATGGCGCGGATATAGAGGACAAATCCGGTTCTTACGCAC
AGATTTCCACCTATGTAAAAGAGGTACGCCAAAATAATAAAAATGTGGTGTTAGTCGATATCGGTGATGCGATCCAAGAC
AACCAAGTGGAAGTTTTCGCTAAAACGAAAAAATACTACAAAGACAATCCGGTGCCTAAAGTATTAAACGAAATGAAATA
CGACTATTTCGTTTTAGGTAACCACGAATTTAACTTCGGTATGACCGCTTTAAATGAAATCATTAAAGACATTAATGCCA
AAGTATTAACAGCTAACTTCTACTACAAAAAAGACGGAAAACGTTACGTTACCGCAACCGATATTATTGAAAAAGACGGC
GTAAAACTCGGTGTAATCGGCTTAACCACCCCAATGGCGGCAACCTTTGAAAAAGATACGCACAATCTTGATGAAATGAA
ATTCAGCTCGCCGAGTGAAGAAGCGAAAGCTCAAATTGCCGAGTTAAAAGCGAAAGGGGTTGATGCGATTATCGTATTAG
CACATATGGGTATTGATAACGAAAACAATATTCCTGATACCGGCGTGGCAGATTTAGTCAATAATGTGGAAGGCATTGAT
GTGATTATCGCCGGTCATATGCACAAAAACATTCCTGCGGAAACGATTAAAAATACGCTAATTACCGAACCGCACCGCTA
CGGTACTGTGGTTTCAGAAGTGGATTTAGCCTTTGATGTCGCGGATAACGGCAAAGTGAAATTATTGTCAAAAACCGCCA
AAACCGTACCGGTTAAGAAATTGGCTTCAGATCCGGCAATCGAAAAAATTTACCAACCGTATCATGACGAATTACGCCGT
TTAAATAATGTGAAAATCGGCGAAACTGCACAAAATATGATTCCGCAGGGTAAAAATCATGGTGTAGCGATTGCCTTTAC
GCAAGATACCGGCTTATCTTCATTGATTAATGACGTGCAACAACATTACAGCAAAGCGGATGTGGTCTCTTTTGCTTTCG
ACCACCAAACCGTACGTTTAGACAAAGGCGATATTAAGAAAAAAGATATTATCTATAACTATCGCTATGCAGGCGGTGAT
GTGAGCGTGTACGAAGTGACCGGCAAACAGCTTAAAGCCTATATGAATTGGTCGGCAGATTACTTCGATACGATTCAGCC
GCAAGATAAAGACTACCGTATCAATGAAAAACGCGGCAAATCGAAATATGTCACGTTTGATATTTTCGGCGGCGTAAAAT
TCAACATTGATTTACGCAAACCAAGCGGCGAGAAAATTGTTGATCTTAGCCTTGCCAACGGTACCGCAATCACTGATGAC
AGCAAAATCAAACTCGGTATGAATTCATATCGCTATGAACAGCTCATCAAAAAAGGTGGTGTATGGGAAGGACAACAAAT
CCCGGTATTATGGGAATCTAAAGTCGCAATGGGCAGCGAAGCCGGCACCATTCAAAATATGATGATCGATTACATCTCTA
ACGTGAAAAAAGGCAAAGTGGAAGGCGTGTCTCACAACTGCTGGAAAATTGTCGGTTTAGAATAA

Upstream 100 bases:

>100_bases
ACCTTTTCTAAAAAACACTTTCAATTAAGTACAATATATAATCAAGCTAAGTTAAAATAGTTTGTAATTTATTTCCTACT
TATCTCATAAGGAGATCATC

Downstream 100 bases:

>100_bases
TTAATACAAAACAAGCGGTTAAATTCTCCAACTTTTACAAAAACCATCAGGAAATATGACCGCTTACAATAAGAATCCAC
TTATCTAAAAACAAAGGGAT

Product: Ser/Thr protein phosphatase family protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MKKVLGTLLVLSAASVAVAKEVNIKFLGTSDIHGRIVPWNYGADIEDKSGSYAQISTYVKEVRQNNKNVVLVDIGDAIQD
NQVEVFAKTKKYYKDNPVPKVLNEMKYDYFVLGNHEFNFGMTALNEIIKDINAKVLTANFYYKKDGKRYVTATDIIEKDG
VKLGVIGLTTPMAATFEKDTHNLDEMKFSSPSEEAKAQIAELKAKGVDAIIVLAHMGIDNENNIPDTGVADLVNNVEGID
VIIAGHMHKNIPAETIKNTLITEPHRYGTVVSEVDLAFDVADNGKVKLLSKTAKTVPVKKLASDPAIEKIYQPYHDELRR
LNNVKIGETAQNMIPQGKNHGVAIAFTQDTGLSSLINDVQQHYSKADVVSFAFDHQTVRLDKGDIKKKDIIYNYRYAGGD
VSVYEVTGKQLKAYMNWSADYFDTIQPQDKDYRINEKRGKSKYVTFDIFGGVKFNIDLRKPSGEKIVDLSLANGTAITDD
SKIKLGMNSYRYEQLIKKGGVWEGQQIPVLWESKVAMGSEAGTIQNMMIDYISNVKKGKVEGVSHNCWKIVGLE

Sequences:

>Translated_554_residues
MKKVLGTLLVLSAASVAVAKEVNIKFLGTSDIHGRIVPWNYGADIEDKSGSYAQISTYVKEVRQNNKNVVLVDIGDAIQD
NQVEVFAKTKKYYKDNPVPKVLNEMKYDYFVLGNHEFNFGMTALNEIIKDINAKVLTANFYYKKDGKRYVTATDIIEKDG
VKLGVIGLTTPMAATFEKDTHNLDEMKFSSPSEEAKAQIAELKAKGVDAIIVLAHMGIDNENNIPDTGVADLVNNVEGID
VIIAGHMHKNIPAETIKNTLITEPHRYGTVVSEVDLAFDVADNGKVKLLSKTAKTVPVKKLASDPAIEKIYQPYHDELRR
LNNVKIGETAQNMIPQGKNHGVAIAFTQDTGLSSLINDVQQHYSKADVVSFAFDHQTVRLDKGDIKKKDIIYNYRYAGGD
VSVYEVTGKQLKAYMNWSADYFDTIQPQDKDYRINEKRGKSKYVTFDIFGGVKFNIDLRKPSGEKIVDLSLANGTAITDD
SKIKLGMNSYRYEQLIKKGGVWEGQQIPVLWESKVAMGSEAGTIQNMMIDYISNVKKGKVEGVSHNCWKIVGLE
>Mature_554_residues
MKKVLGTLLVLSAASVAVAKEVNIKFLGTSDIHGRIVPWNYGADIEDKSGSYAQISTYVKEVRQNNKNVVLVDIGDAIQD
NQVEVFAKTKKYYKDNPVPKVLNEMKYDYFVLGNHEFNFGMTALNEIIKDINAKVLTANFYYKKDGKRYVTATDIIEKDG
VKLGVIGLTTPMAATFEKDTHNLDEMKFSSPSEEAKAQIAELKAKGVDAIIVLAHMGIDNENNIPDTGVADLVNNVEGID
VIIAGHMHKNIPAETIKNTLITEPHRYGTVVSEVDLAFDVADNGKVKLLSKTAKTVPVKKLASDPAIEKIYQPYHDELRR
LNNVKIGETAQNMIPQGKNHGVAIAFTQDTGLSSLINDVQQHYSKADVVSFAFDHQTVRLDKGDIKKKDIIYNYRYAGGD
VSVYEVTGKQLKAYMNWSADYFDTIQPQDKDYRINEKRGKSKYVTFDIFGGVKFNIDLRKPSGEKIVDLSLANGTAITDD
SKIKLGMNSYRYEQLIKKGGVWEGQQIPVLWESKVAMGSEAGTIQNMMIDYISNVKKGKVEGVSHNCWKIVGLE

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=625, Percent_Identity=25.12, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1786687, Length=458, Percent_Identity=24.6724890829694, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24641187, Length=496, Percent_Identity=21.5725806451613, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221329836, Length=350, Percent_Identity=23.1428571428571, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19922446, Length=199, Percent_Identity=27.1356783919598, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24654424, Length=199, Percent_Identity=27.1356783919598, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922444, Length=224, Percent_Identity=26.3392857142857, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 61654; Mature: 61654

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLGTLLVLSAASVAVAKEVNIKFLGTSDIHGRIVPWNYGADIEDKSGSYAQISTYVK
CHHHHHHHHHHHHHHHEEEEECEEEEEECCCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
EVRQNNKNVVLVDIGDAIQDNQVEVFAKTKKYYKDNPVPKVLNEMKYDYFVLGNHEFNFG
HHHHCCCCEEEEECCCCCCCCCEEEEEEHHHHHCCCCHHHHHHHCCCCEEEECCCCCCCC
MTALNEIIKDINAKVLTANFYYKKDGKRYVTATDIIEKDGVKLGVIGLTTPMAATFEKDT
HHHHHHHHHHCCCEEEEEEEEEECCCCEEEEEEHHHHCCCCEEEEEEECCCCHHHHCCCC
HNLDEMKFSSPSEEAKAQIAELKAKGVDAIIVLAHMGIDNENNIPDTGVADLVNNVEGID
CCHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHCCCCCEE
VIIAGHMHKNIPAETIKNTLITEPHRYGTVVSEVDLAFDVADNGKVKLLSKTAKTVPVKK
EEEECCCCCCCCHHHHHHHHCCCCHHHCCHHEEEEEEEEECCCCCEEEEECCCCCCCHHH
LASDPAIEKIYQPYHDELRRLNNVKIGETAQNMIPQGKNHGVAIAFTQDTGLSSLINDVQ
HCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHH
QHYSKADVVSFAFDHQTVRLDKGDIKKKDIIYNYRYAGGDVSVYEVTGKQLKAYMNWSAD
HHHCHHHEEEEEECCCEEEECCCCCCCCEEEEEEEECCCCEEEEEECCHHHHHHHCCCCC
YFDTIQPQDKDYRINEKRGKSKYVTFDIFGGVKFNIDLRKPSGEKIVDLSLANGTAITDD
HHCCCCCCCCCEEECCCCCCCEEEEEEEECCEEEEEEEECCCCCEEEEEEECCCCEECCC
SKIKLGMNSYRYEQLIKKGGVWEGQQIPVLWESKVAMGSEAGTIQNMMIDYISNVKKGKV
CEEEECCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCC
EGVSHNCWKIVGLE
CCCCCCCEEEEECC
>Mature Secondary Structure
MKKVLGTLLVLSAASVAVAKEVNIKFLGTSDIHGRIVPWNYGADIEDKSGSYAQISTYVK
CHHHHHHHHHHHHHHHEEEEECEEEEEECCCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
EVRQNNKNVVLVDIGDAIQDNQVEVFAKTKKYYKDNPVPKVLNEMKYDYFVLGNHEFNFG
HHHHCCCCEEEEECCCCCCCCCEEEEEEHHHHHCCCCHHHHHHHCCCCEEEECCCCCCCC
MTALNEIIKDINAKVLTANFYYKKDGKRYVTATDIIEKDGVKLGVIGLTTPMAATFEKDT
HHHHHHHHHHCCCEEEEEEEEEECCCCEEEEEEHHHHCCCCEEEEEEECCCCHHHHCCCC
HNLDEMKFSSPSEEAKAQIAELKAKGVDAIIVLAHMGIDNENNIPDTGVADLVNNVEGID
CCHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHCCCCCEE
VIIAGHMHKNIPAETIKNTLITEPHRYGTVVSEVDLAFDVADNGKVKLLSKTAKTVPVKK
EEEECCCCCCCCHHHHHHHHCCCCHHHCCHHEEEEEEEEECCCCCEEEEECCCCCCCHHH
LASDPAIEKIYQPYHDELRRLNNVKIGETAQNMIPQGKNHGVAIAFTQDTGLSSLINDVQ
HCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHH
QHYSKADVVSFAFDHQTVRLDKGDIKKKDIIYNYRYAGGDVSVYEVTGKQLKAYMNWSAD
HHHCHHHEEEEEECCCEEEECCCCCCCCEEEEEEEECCCCEEEEEECCHHHHHHHCCCCC
YFDTIQPQDKDYRINEKRGKSKYVTFDIFGGVKFNIDLRKPSGEKIVDLSLANGTAITDD
HHCCCCCCCCCEEECCCCCCCEEEEEEEECCEEEEEEEECCCCCEEEEEEECCCCEECCC
SKIKLGMNSYRYEQLIKKGGVWEGQQIPVLWESKVAMGSEAGTIQNMMIDYISNVKKGKV
CEEEECCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCC
EGVSHNCWKIVGLE
CCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]