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The map label for this gene is ubiF [H]

Identifier: 126209208

GI number: 126209208

Start: 1981355

End: 1982521

Strand: Reverse

Name: ubiF [H]

Synonym: APL_1744

Alternate gene names: 126209208

Gene position: 1982521-1981355 (Counterclockwise)

Preceding gene: 126209209

Following gene: 126209207

Centisome position: 87.16

GC content: 45.76

Gene sequence:

>1167_bases
ATGAAAACACAGGATATTATCATTATCGGTGGCGGTATGGTCGGAGCTGCGGTAGCACTCGGCTTGGCGAAACAAGGGTT
AAATATTGCATTAATCGAAAAAAATCCGCTTCCCTCATTTGATGCGAATGCTGCTTATGATTTACGCATTTCCGCTATCA
GTATTACCTCGGTCAAACTGCTGGAAGAACTGGGTGCTTGGCAGGCGATCAGCCAAATGCGAGTTTGCCCGTATGACGGT
TTGGAAACTTGGGAAATCGAAGGGTTTAATACCGCCTTTTATGCAACGGAAATCGGCTTGGATAAACTCGGTTTTATGGT
GGAAAATAACGCGATTCAGCTCGGTTTATGGCAAGCCTTAAACCAGTATCCGAATTGCCGACAAGCGGTCGGATTTTCGC
AAATTTCTGCAAATTATCACGAACAATTATGGACGGTCACCGTTGATGATCAAACATTTACCGCCCCACTACTGATCGCT
GCGGACGGCGCAAACTCACAAGTGCGTAACTGGGCGGGTATCGGGCTAACCGGTTGGCAATATCGCCAACATTGCTTGCT
TGCCACGGTGAAAACCGAATTACCGCAACAATCTGTCACCTGGCAACAATTCTTCCCGAGCGGCCCTCGTGCATTTTTAC
CTCTATCAGATCACAACGGTTGTGTCGTGTGGTATGACGCACCGCAACGCATTGCTCAGCTAAAACAACTGCCTTCAGAA
AAACTCACCGCCGAAATTCAACAACATTTCCCTGCCCGTTTAGGCAAAGTGGGAGTAGTCAATGCCGCCGGTTTTCCACT
GACTCGCCAACACGCTCAACATTATGTAAAAAACGGCGTGGTGTTGATGGGTGATGCGGCGCACACTATTAATCCGCTTG
CCGGACAAGGGGTTAATCTCGGTTTTAAAGACGTGCAAGTATTATTGGAAGTAATTGAACAAGCGGTTAAAAAAGACGAA
AACTTTGCAAATGAAGCGGTACTTAAACGTTATGAACATAAACGTAAACCCGACAATTTATTGATGCAAACCGGTATGGA
CGTTTTTTATAAGGCATTCAAAACCGAGTTATTGCCGGTAAAAGCCGCCCGAAATTTAGGTTTAGTACTGGCGGAAAAAA
TCACGCCTCTTAAGAAAAAAGCACTACGCTATGCGATAGGTTTATAG

Upstream 100 bases:

>100_bases
TGAAACTATCCTAAAAATCCTAAAAGCACTAAATATTGAGTTAGTTCCGGTGATTAAAACGTTATAATAGGGCATCAATA
TTCAGCAAATGAGGGGCAAA

Downstream 100 bases:

>100_bases
CTCTCATCACAAGCGGTCAAGTTTTGCAGATTTTTTGCGAAATTCGACCGCTTGCTGTTTTCGCAAACACTCAATTTAAG
TCAATAACGTTACCTTTTCT

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKTQDIIIIGGGMVGAAVALGLAKQGLNIALIEKNPLPSFDANAAYDLRISAISITSVKLLEELGAWQAISQMRVCPYDG
LETWEIEGFNTAFYATEIGLDKLGFMVENNAIQLGLWQALNQYPNCRQAVGFSQISANYHEQLWTVTVDDQTFTAPLLIA
ADGANSQVRNWAGIGLTGWQYRQHCLLATVKTELPQQSVTWQQFFPSGPRAFLPLSDHNGCVVWYDAPQRIAQLKQLPSE
KLTAEIQQHFPARLGKVGVVNAAGFPLTRQHAQHYVKNGVVLMGDAAHTINPLAGQGVNLGFKDVQVLLEVIEQAVKKDE
NFANEAVLKRYEHKRKPDNLLMQTGMDVFYKAFKTELLPVKAARNLGLVLAEKITPLKKKALRYAIGL

Sequences:

>Translated_388_residues
MKTQDIIIIGGGMVGAAVALGLAKQGLNIALIEKNPLPSFDANAAYDLRISAISITSVKLLEELGAWQAISQMRVCPYDG
LETWEIEGFNTAFYATEIGLDKLGFMVENNAIQLGLWQALNQYPNCRQAVGFSQISANYHEQLWTVTVDDQTFTAPLLIA
ADGANSQVRNWAGIGLTGWQYRQHCLLATVKTELPQQSVTWQQFFPSGPRAFLPLSDHNGCVVWYDAPQRIAQLKQLPSE
KLTAEIQQHFPARLGKVGVVNAAGFPLTRQHAQHYVKNGVVLMGDAAHTINPLAGQGVNLGFKDVQVLLEVIEQAVKKDE
NFANEAVLKRYEHKRKPDNLLMQTGMDVFYKAFKTELLPVKAARNLGLVLAEKITPLKKKALRYAIGL
>Mature_388_residues
MKTQDIIIIGGGMVGAAVALGLAKQGLNIALIEKNPLPSFDANAAYDLRISAISITSVKLLEELGAWQAISQMRVCPYDG
LETWEIEGFNTAFYATEIGLDKLGFMVENNAIQLGLWQALNQYPNCRQAVGFSQISANYHEQLWTVTVDDQTFTAPLLIA
ADGANSQVRNWAGIGLTGWQYRQHCLLATVKTELPQQSVTWQQFFPSGPRAFLPLSDHNGCVVWYDAPQRIAQLKQLPSE
KLTAEIQQHFPARLGKVGVVNAAGFPLTRQHAQHYVKNGVVLMGDAAHTINPLAGQGVNLGFKDVQVLLEVIEQAVKKDE
NFANEAVLKRYEHKRKPDNLLMQTGMDVFYKAFKTELLPVKAARNLGLVLAEKITPLKKKALRYAIGL

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=404, Percent_Identity=29.4554455445545, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI32967307, Length=332, Percent_Identity=30.1204819277108, Blast_Score=149, Evalue=6e-36,
Organism=Escherichia coli, GI1786883, Length=385, Percent_Identity=50.3896103896104, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1789273, Length=393, Percent_Identity=38.676844783715, Blast_Score=254, Evalue=5e-69,
Organism=Escherichia coli, GI1789274, Length=397, Percent_Identity=29.7229219143577, Blast_Score=164, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI25146631, Length=436, Percent_Identity=29.8165137614679, Blast_Score=185, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321694, Length=448, Percent_Identity=27.0089285714286, Blast_Score=153, Evalue=4e-38,
Organism=Drosophila melanogaster, GI19920760, Length=426, Percent_Identity=29.3427230046948, Blast_Score=174, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 42837; Mature: 42837

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTQDIIIIGGGMVGAAVALGLAKQGLNIALIEKNPLPSFDANAAYDLRISAISITSVKL
CCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEEEEEEHHHHHH
LEELGAWQAISQMRVCPYDGLETWEIEGFNTAFYATEIGLDKLGFMVENNAIQLGLWQAL
HHHHCHHHHHHHCCCCCCCCCCEEEECCCCEEEEEEECCHHHHCEEEECCEEEEHHHHHH
NQYPNCRQAVGFSQISANYHEQLWTVTVDDQTFTAPLLIAADGANSQVRNWAGIGLTGWQ
HHCCCHHHHCCHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHCCCCCCCCHH
YRQHCLLATVKTELPQQSVTWQQFFPSGPRAFLPLSDHNGCVVWYDAPQRIAQLKQLPSE
HHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEECCCCCEEEEECCHHHHHHHHHCCHH
KLTAEIQQHFPARLGKVGVVNAAGFPLTRQHAQHYVKNGVVLMGDAAHTINPLAGQGVNL
HHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCEEEEECCHHHCCCCCCCCCCC
GFKDVQVLLEVIEQAVKKDENFANEAVLKRYEHKRKPDNLLMQTGMDVFYKAFKTELLPV
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCH
KAARNLGLVLAEKITPLKKKALRYAIGL
HHHCCCCCHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKTQDIIIIGGGMVGAAVALGLAKQGLNIALIEKNPLPSFDANAAYDLRISAISITSVKL
CCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEEEEEEHHHHHH
LEELGAWQAISQMRVCPYDGLETWEIEGFNTAFYATEIGLDKLGFMVENNAIQLGLWQAL
HHHHCHHHHHHHCCCCCCCCCCEEEECCCCEEEEEEECCHHHHCEEEECCEEEEHHHHHH
NQYPNCRQAVGFSQISANYHEQLWTVTVDDQTFTAPLLIAADGANSQVRNWAGIGLTGWQ
HHCCCHHHHCCHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHCCCCCCCCHH
YRQHCLLATVKTELPQQSVTWQQFFPSGPRAFLPLSDHNGCVVWYDAPQRIAQLKQLPSE
HHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEECCCCCEEEEECCHHHHHHHHHCCHH
KLTAEIQQHFPARLGKVGVVNAAGFPLTRQHAQHYVKNGVVLMGDAAHTINPLAGQGVNL
HHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCEEEEECCHHHCCCCCCCCCCC
GFKDVQVLLEVIEQAVKKDENFANEAVLKRYEHKRKPDNLLMQTGMDVFYKAFKTELLPV
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCH
KAARNLGLVLAEKITPLKKKALRYAIGL
HHHCCCCCHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]