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The map label for this gene is napF [C]

Identifier: 126209142

GI number: 126209142

Start: 1904636

End: 1905145

Strand: Direct

Name: napF [C]

Synonym: APL_1678

Alternate gene names: 126209142

Gene position: 1904636-1905145 (Clockwise)

Preceding gene: 126209141

Following gene: 126209147

Centisome position: 83.74

GC content: 43.53

Gene sequence:

>510_bases
ATGTTAGGTAGAAATAAATTAACGCTACCGGAATTAACACCGAGAGTATTTTCTCGGAGAGAACTGTTTACCGGCTTTTT
GCGTCAAACTCAATCTCATCAAGAGCAAACTCGAGTTGAAAATCGACCGCCTTTTGCAGCACCGGAACATTTGTTTAGTG
TAGCCTGTGACGGCTGTAGTAAATGTGTCACGGCTTGCCCGGTAGGGGTTATTGATATTCGCCGACAACAAGCGGTATTA
GATTTAACTTTTTCCGCTTGTACACTTTGTGGCAAATGTGCCGAAAGCTGCCCGACTCAAGCCCTACATCTCTCTTTTAA
AAAAGATACAGAACTCCGTCCCCAATTCTCATCCGCTTGTTTACAAACAAAAGGACAACCTTGCGATAGTTGTATCCGAA
GCTGCCCTCAACAAGCGATTTCATCTCAACTCACCATTAATAACGAACTTTGTAACGGTTGCGGCGAATGTAAACAAGCC
TGTTTTATGGCGGCAGTGAGCTTAAAGTAA

Upstream 100 bases:

>100_bases
GTTCTATAAAGGTTTAGCGTTACTGGCTCAACAAACCTTAAAAGATTGGCAACAACAGTTGGCAATTATTGTTCCTCAAG
TTTCTTTTTATCGTTAAGCT

Downstream 100 bases:

>100_bases
TTAAAAACAAGTAATCTGTAATAAATGCTTGTTTATGTCACAATTATTTAAGAAAGGAAGCCATCAAGTCTTTTGAGATC
GGCATAAGACCTAGCTTTAG

Product: putative ferredoxin

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MLGRNKLTLPELTPRVFSRRELFTGFLRQTQSHQEQTRVENRPPFAAPEHLFSVACDGCSKCVTACPVGVIDIRRQQAVL
DLTFSACTLCGKCAESCPTQALHLSFKKDTELRPQFSSACLQTKGQPCDSCIRSCPQQAISSQLTINNELCNGCGECKQA
CFMAAVSLK

Sequences:

>Translated_169_residues
MLGRNKLTLPELTPRVFSRRELFTGFLRQTQSHQEQTRVENRPPFAAPEHLFSVACDGCSKCVTACPVGVIDIRRQQAVL
DLTFSACTLCGKCAESCPTQALHLSFKKDTELRPQFSSACLQTKGQPCDSCIRSCPQQAISSQLTINNELCNGCGECKQA
CFMAAVSLK
>Mature_169_residues
MLGRNKLTLPELTPRVFSRRELFTGFLRQTQSHQEQTRVENRPPFAAPEHLFSVACDGCSKCVTACPVGVIDIRRQQAVL
DLTFSACTLCGKCAESCPTQALHLSFKKDTELRPQFSSACLQTKGQPCDSCIRSCPQQAISSQLTINNELCNGCGECKQA
CFMAAVSLK

Specific function: Involved In Electron Transfer. [C]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788536, Length=162, Percent_Identity=27.7777777777778, Blast_Score=64, Evalue=4e-12,
Organism=Escherichia coli, GI1788533, Length=173, Percent_Identity=28.3236994219653, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004496 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 18579; Mature: 18579

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
10.7 %Cys+Met (Translated Protein)
9.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
10.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGRNKLTLPELTPRVFSRRELFTGFLRQTQSHQEQTRVENRPPFAAPEHLFSVACDGCS
CCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
KCVTACPVGVIDIRRQQAVLDLTFSACTLCGKCAESCPTQALHLSFKKDTELRPQFSSAC
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHH
LQTKGQPCDSCIRSCPQQAISSQLTINNELCNGCGECKQACFMAAVSLK
HHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLGRNKLTLPELTPRVFSRRELFTGFLRQTQSHQEQTRVENRPPFAAPEHLFSVACDGCS
CCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
KCVTACPVGVIDIRRQQAVLDLTFSACTLCGKCAESCPTQALHLSFKKDTELRPQFSSAC
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHH
LQTKGQPCDSCIRSCPQQAISSQLTINNELCNGCGECKQACFMAAVSLK
HHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]