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The map label for this gene is gidB

Identifier: 126209118

GI number: 126209118

Start: 1880642

End: 1881259

Strand: Reverse

Name: gidB

Synonym: APL_1654

Alternate gene names: 126209118

Gene position: 1881259-1880642 (Counterclockwise)

Preceding gene: 126209119

Following gene: 126209117

Centisome position: 82.71

GC content: 40.13

Gene sequence:

>618_bases
ATGAGACAGAAATTAGACCGCTTGTTGGAACAGGCGCAAATTAATTTGACCGATCAGCAGAAAGAGCAGTTGGTAGGTTT
CGTGCGTTTATTAGATAAATGGAATAAAGCTTATAACCTGACTTCGGTGCGTAATCCTGACGAAATGCTGGTTAAGCATA
TCTTAGATAGTTTAGTGGTAAGCGAGCATTTACAGGGAAATAATTTTATTGATGTCGGCACCGGGCCGGGATTACCTGGC
ATACCGCTTGCGATTGCAAATCCGGATAAACAATTTGTATTGCTGGATAGTCTTGGTAAGCGTATTACTTTTATCAAGAA
CGCATTACGTGAGTTAGGTATTACTAATGTAACTCCGGTACTGTCTCGTGTGGAAGAGTATAAGGAACAAACGTTTGACG
GGGTATTAAGCCGAGCTTTTGCCAGCTTAAACGATATGGTGGATTGGTGTTACCATTTACCGAATCCGCAAGGTAAATTT
TATGCGCTTAAAGGCATTTATGCGGAAAGTGAAGTGCAAGAGATTAAAAATCCGATTGGGCTGGAAAAAGTCATTCCTCT
TTCCGTACCGGAATTAGTTGGTGAGCGTCATTTAGTATTATTAAATAAACCTAATTAA

Upstream 100 bases:

>100_bases
ATTTAGCTGATTAGGAAACACGGAATATACGGAAATTAAAACAATGATTACTTTCGGTTTATTCCGTGTTTTATTGTGTA
AACCATAAGGATTTTACAAA

Downstream 100 bases:

>100_bases
GCTGATTTACTGAGATGATCTCTTTGAGATAAAAAATTAAGTAAAAAGTATCACTTTACACTTATTTTTTTTGAGTTGTT
AATCTATGAAAAATATTTAA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MRQKLDRLLEQAQINLTDQQKEQLVGFVRLLDKWNKAYNLTSVRNPDEMLVKHILDSLVVSEHLQGNNFIDVGTGPGLPG
IPLAIANPDKQFVLLDSLGKRITFIKNALRELGITNVTPVLSRVEEYKEQTFDGVLSRAFASLNDMVDWCYHLPNPQGKF
YALKGIYAESEVQEIKNPIGLEKVIPLSVPELVGERHLVLLNKPN

Sequences:

>Translated_205_residues
MRQKLDRLLEQAQINLTDQQKEQLVGFVRLLDKWNKAYNLTSVRNPDEMLVKHILDSLVVSEHLQGNNFIDVGTGPGLPG
IPLAIANPDKQFVLLDSLGKRITFIKNALRELGITNVTPVLSRVEEYKEQTFDGVLSRAFASLNDMVDWCYHLPNPQGKF
YALKGIYAESEVQEIKNPIGLEKVIPLSVPELVGERHLVLLNKPN
>Mature_205_residues
MRQKLDRLLEQAQINLTDQQKEQLVGFVRLLDKWNKAYNLTSVRNPDEMLVKHILDSLVVSEHLQGNNFIDVGTGPGLPG
IPLAIANPDKQFVLLDSLGKRITFIKNALRELGITNVTPVLSRVEEYKEQTFDGVLSRAFASLNDMVDWCYHLPNPQGKF
YALKGIYAESEVQEIKNPIGLEKVIPLSVPELVGERHLVLLNKPN

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=202, Percent_Identity=60.8910891089109, Blast_Score=252, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_ACTP2 (A3N2V2)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054343.1
- ProteinModelPortal:   A3N2V2
- SMR:   A3N2V2
- STRING:   A3N2V2
- GeneID:   4848985
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1654
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- PhylomeDB:   A3N2V2
- ProtClustDB:   PRK00107
- BioCyc:   APLE416269:APL_1654-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 23138; Mature: 23138

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQKLDRLLEQAQINLTDQQKEQLVGFVRLLDKWNKAYNLTSVRNPDEMLVKHILDSLVV
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
SEHLQGNNFIDVGTGPGLPGIPLAIANPDKQFVLLDSLGKRITFIKNALRELGITNVTPV
HHHCCCCCEEECCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHH
LSRVEEYKEQTFDGVLSRAFASLNDMVDWCYHLPNPQGKFYALKGIYAESEVQEIKNPIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCC
LEKVIPLSVPELVGERHLVLLNKPN
CHHCCCCCCHHHHCCCEEEEEECCC
>Mature Secondary Structure
MRQKLDRLLEQAQINLTDQQKEQLVGFVRLLDKWNKAYNLTSVRNPDEMLVKHILDSLVV
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
SEHLQGNNFIDVGTGPGLPGIPLAIANPDKQFVLLDSLGKRITFIKNALRELGITNVTPV
HHHCCCCCEEECCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHH
LSRVEEYKEQTFDGVLSRAFASLNDMVDWCYHLPNPQGKFYALKGIYAESEVQEIKNPIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCC
LEKVIPLSVPELVGERHLVLLNKPN
CHHCCCCCCHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA