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The map label for this gene is gidA

Identifier: 126209119

GI number: 126209119

Start: 1881359

End: 1883251

Strand: Reverse

Name: gidA

Synonym: APL_1655

Alternate gene names: 126209119

Gene position: 1883251-1881359 (Counterclockwise)

Preceding gene: 126209121

Following gene: 126209118

Centisome position: 82.8

GC content: 44.9

Gene sequence:

>1893_bases
ATGATTTACCATGAAATTTATGATGTGATCGTTGTTGGCGGCGGTCATGCAGGGACGGAAGCGGCACTGGCTCCTGCCCG
TATGGGGTTAAAAACGCTACTTCTAACACATAATGTAGATACTTTAGGGCAAATGTCTTGTAATCCGGCTATCGGCGGTA
TCGGTAAAGGTCATTTAGTAAAAGAGATCGATGCGATGGGCGGTTTAATGGCCATTGCTATTGACCAAGCGGGGATCCAA
TTCCGTACCTTAAACAGTTCGAAAGGGCCTGCCGTACGTGCGACTCGTGCGCAAGCAGACCGAGTGCTTTACCGCCAAGC
GGTGCGTACCGCACTAGAAAATCAACCTAATTTAGATATTTTCCAACAAGAAGTGGTGGATATTTTAGTCGAAAATAACC
GTGCGGTTGGGGCTGTAACTAAAATGGGGCTCACCTTTAAAGCACGTTCCGTAGTATTAACCGCCGGTACTTTCCTTGCC
GGTAAAATTCATATCGGTTTAGATAACTATGCCGGAGGGCGTGCGGGAGATCCTGCTGCGACAATGTTGGCAGATCGCCT
ACGTGATCTGAATTTACGTGTTGATCGTCTTAAAACCGGTACGCCGCCGCGTTTGGATGCTCGTACGATCAATTTTGACG
TTTTGGCTAAACAACATGGTGATGCGGAATTACCGGTAATGTCGTTTATGGGGTCGGTAGATCTTCATCCGCGCCAAATC
CCTTGCCATATCACGCATACTAATGAACAAACACACGATTTGATCCGAAATAGTTTAGATCGTAGTCCGATGTACACCGG
TATCATCGAAGGGATCGGACCTCGTTACTGTCCGTCGATCGAAGATAAAGTAATGCGTTTCAGCGATCGTAACAGTCACC
AAATTTATCTTGAGCCTGAAGGTTTAAGTACCATTGAAGTTTACCCGAACGGGATTTCAACCAGTTTGCCGTTTGACGTA
CAAATGGGTATCGTAAATTCGATGAAGGGTTTGGAAAAAACTCGTATTATCAAACCGGGCTATGCAATTGAATATGATTA
TTTCGACCCTCGTGATCTAAAACCGACGTTAGAAACTAAGGCGATTGAAGGTTTATTCTTTGCAGGTCAGATTAACGGTA
CGACCGGTTATGAAGAAGCTGCTGCACAAGGTTTGTTAGCCGGTATTAATGCGGCATTACAGGTTCAAGGCAAAGAGGCT
TGGTTCCCAACTCGTGATCTTGCTTATACAGGCGTATTGGTTGATGATCTTTGCACGCTCGGTACTAAAGAGCCGTACCG
TGTCTTTACCTCGCGTGCGGAATATCGTTTATTGCTGCGTGAAGATAATGCAGATATCCGTTTGACCCCGATTGCACATG
AGTTAGGTTTGATTGATGATGCGCGTTGGGCGAGATTCAATCAGAAAATGGAAAATATCGAACGTGAGCGTGAACGTCTA
AAACAAATTTGGATTCACCCGCAATCGGAACATTTGGCGGTAGTCAATGAATTGGTTAATAGTCCGCTTACCCGTGAAGC
AAGCGGCGAGGATTTATTACGCCGTCCAGAAGTGACTTATGACAAATTAACCCAAGTCGCTGCGTTTGCTCCGGCTTTAG
ACGATAAACAAGCGGCTGAACAAGTTGAAATATCGATTAAATATCAGGGTTATATCGAACATCAGCAGAATGAGATTGAA
CGTCATAAACGTCACGAAAATACTTTAATTCCGGCTGAATTTGATTATGACAAGGTTGAGAGCCTTTCAAACGAAGTACG
AGCGAAACTGATGCAACATCGCCCGGTTTCGATTGGCCAAGCAAGTCGTATTTCAGGTATTACACCGGCCGCGATTTCAA
TTCTTCTCGTAAATTTGAAAAAGCAAGGCATGCTGAAACGAGGCGAATTATAA

Upstream 100 bases:

>100_bases
ACGATCTTAAAATTGCGTTTTCATGATCCTGTAATTTTGAGGTAGAATAGCACTCTTTTGTTTCAGTTACTCAGAATATA
AAAATTATAAAGGCAGATAT

Downstream 100 bases:

>100_bases
TTTAGCTGATTAGGAAACACGGAATATACGGAAATTAAAACAATGATTACTTTCGGTTTATTCCGTGTTTTATTGTGTAA
ACCATAAGGATTTTACAAAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MIYHEIYDVIVVGGGHAGTEAALAPARMGLKTLLLTHNVDTLGQMSCNPAIGGIGKGHLVKEIDAMGGLMAIAIDQAGIQ
FRTLNSSKGPAVRATRAQADRVLYRQAVRTALENQPNLDIFQQEVVDILVENNRAVGAVTKMGLTFKARSVVLTAGTFLA
GKIHIGLDNYAGGRAGDPAATMLADRLRDLNLRVDRLKTGTPPRLDARTINFDVLAKQHGDAELPVMSFMGSVDLHPRQI
PCHITHTNEQTHDLIRNSLDRSPMYTGIIEGIGPRYCPSIEDKVMRFSDRNSHQIYLEPEGLSTIEVYPNGISTSLPFDV
QMGIVNSMKGLEKTRIIKPGYAIEYDYFDPRDLKPTLETKAIEGLFFAGQINGTTGYEEAAAQGLLAGINAALQVQGKEA
WFPTRDLAYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTPIAHELGLIDDARWARFNQKMENIERERERL
KQIWIHPQSEHLAVVNELVNSPLTREASGEDLLRRPEVTYDKLTQVAAFAPALDDKQAAEQVEISIKYQGYIEHQQNEIE
RHKRHENTLIPAEFDYDKVESLSNEVRAKLMQHRPVSIGQASRISGITPAAISILLVNLKKQGMLKRGEL

Sequences:

>Translated_630_residues
MIYHEIYDVIVVGGGHAGTEAALAPARMGLKTLLLTHNVDTLGQMSCNPAIGGIGKGHLVKEIDAMGGLMAIAIDQAGIQ
FRTLNSSKGPAVRATRAQADRVLYRQAVRTALENQPNLDIFQQEVVDILVENNRAVGAVTKMGLTFKARSVVLTAGTFLA
GKIHIGLDNYAGGRAGDPAATMLADRLRDLNLRVDRLKTGTPPRLDARTINFDVLAKQHGDAELPVMSFMGSVDLHPRQI
PCHITHTNEQTHDLIRNSLDRSPMYTGIIEGIGPRYCPSIEDKVMRFSDRNSHQIYLEPEGLSTIEVYPNGISTSLPFDV
QMGIVNSMKGLEKTRIIKPGYAIEYDYFDPRDLKPTLETKAIEGLFFAGQINGTTGYEEAAAQGLLAGINAALQVQGKEA
WFPTRDLAYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTPIAHELGLIDDARWARFNQKMENIERERERL
KQIWIHPQSEHLAVVNELVNSPLTREASGEDLLRRPEVTYDKLTQVAAFAPALDDKQAAEQVEISIKYQGYIEHQQNEIE
RHKRHENTLIPAEFDYDKVESLSNEVRAKLMQHRPVSIGQASRISGITPAAISILLVNLKKQGMLKRGEL
>Mature_630_residues
MIYHEIYDVIVVGGGHAGTEAALAPARMGLKTLLLTHNVDTLGQMSCNPAIGGIGKGHLVKEIDAMGGLMAIAIDQAGIQ
FRTLNSSKGPAVRATRAQADRVLYRQAVRTALENQPNLDIFQQEVVDILVENNRAVGAVTKMGLTFKARSVVLTAGTFLA
GKIHIGLDNYAGGRAGDPAATMLADRLRDLNLRVDRLKTGTPPRLDARTINFDVLAKQHGDAELPVMSFMGSVDLHPRQI
PCHITHTNEQTHDLIRNSLDRSPMYTGIIEGIGPRYCPSIEDKVMRFSDRNSHQIYLEPEGLSTIEVYPNGISTSLPFDV
QMGIVNSMKGLEKTRIIKPGYAIEYDYFDPRDLKPTLETKAIEGLFFAGQINGTTGYEEAAAQGLLAGINAALQVQGKEA
WFPTRDLAYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTPIAHELGLIDDARWARFNQKMENIERERERL
KQIWIHPQSEHLAVVNELVNSPLTREASGEDLLRRPEVTYDKLTQVAAFAPALDDKQAAEQVEISIKYQGYIEHQQNEIE
RHKRHENTLIPAEFDYDKVESLSNEVRAKLMQHRPVSIGQASRISGITPAAISILLVNLKKQGMLKRGEL

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=630, Percent_Identity=44.7619047619048, Blast_Score=494, Evalue=1e-139,
Organism=Homo sapiens, GI19882217, Length=655, Percent_Identity=43.0534351145038, Blast_Score=480, Evalue=1e-135,
Organism=Homo sapiens, GI183227703, Length=670, Percent_Identity=42.089552238806, Blast_Score=474, Evalue=1e-133,
Organism=Escherichia coli, GI2367273, Length=627, Percent_Identity=75.4385964912281, Blast_Score=999, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=624, Percent_Identity=39.1025641025641, Blast_Score=441, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6321202, Length=620, Percent_Identity=43.5483870967742, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24658174, Length=637, Percent_Identity=44.8979591836735, Blast_Score=504, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_ACTP2 (A3N2V3)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054344.1
- ProteinModelPortal:   A3N2V3
- SMR:   A3N2V3
- STRING:   A3N2V3
- GeneID:   4850207
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1655
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- PhylomeDB:   A3N2V3
- ProtClustDB:   PRK05192
- BioCyc:   APLE416269:APL_1655-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 69935; Mature: 69935

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYHEIYDVIVVGGGHAGTEAALAPARMGLKTLLLTHNVDTLGQMSCNPAIGGIGKGHLV
CCHHEEEEEEEECCCCCCCHHHHHHHHHCHHHEEEECCCCHHHCCCCCCCCCCCCCCHHH
KEIDAMGGLMAIAIDQAGIQFRTLNSSKGPAVRATRAQADRVLYRQAVRTALENQPNLDI
HHHHHHCCEEEEEEECCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHH
FQQEVVDILVENNRAVGAVTKMGLTFKARSVVLTAGTFLAGKIHIGLDNYAGGRAGDPAA
HHHHHHHHHHCCCCCEEHHHHCCEEEEECEEEEEECCEEEEEEEEECCCCCCCCCCCHHH
TMLADRLRDLNLRVDRLKTGTPPRLDARTINFDVLAKQHGDAELPVMSFMGSVDLHPRQI
HHHHHHHHHCCCEEEEECCCCCCCCCEEEEEEEEEEECCCCCCCCHHHHCCCCCCCCCCC
PCHITHTNEQTHDLIRNSLDRSPMYTGIIEGIGPRYCPSIEDKVMRFSDRNSHQIYLEPE
EEEEECCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEECCC
GLSTIEVYPNGISTSLPFDVQMGIVNSMKGLEKTRIIKPGYAIEYDYFDPRDLKPTLETK
CCEEEEEECCCCCCCCCCEEEHHHHHHHCCCCHHEEECCCCEEEECCCCCCCCCCCHHHH
AIEGLFFAGQINGTTGYEEAAAQGLLAGINAALQVQGKEAWFPTRDLAYTGVLVDDLCTL
HHCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHEEECHHHHHHHHC
GTKEPYRVFTSRAEYRLLLREDNADIRLTPIAHELGLIDDARWARFNQKMENIERERERL
CCCCCHHHHHCCCEEEEEEEECCCCEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KQIWIHPQSEHLAVVNELVNSPLTREASGEDLLRRPEVTYDKLTQVAAFAPALDDKQAAE
HHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHC
QVEISIKYQGYIEHQQNEIERHKRHENTLIPAEFDYDKVESLSNEVRAKLMQHRPVSIGQ
EEEEEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
ASRISGITPAAISILLVNLKKQGMLKRGEL
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIYHEIYDVIVVGGGHAGTEAALAPARMGLKTLLLTHNVDTLGQMSCNPAIGGIGKGHLV
CCHHEEEEEEEECCCCCCCHHHHHHHHHCHHHEEEECCCCHHHCCCCCCCCCCCCCCHHH
KEIDAMGGLMAIAIDQAGIQFRTLNSSKGPAVRATRAQADRVLYRQAVRTALENQPNLDI
HHHHHHCCEEEEEEECCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHH
FQQEVVDILVENNRAVGAVTKMGLTFKARSVVLTAGTFLAGKIHIGLDNYAGGRAGDPAA
HHHHHHHHHHCCCCCEEHHHHCCEEEEECEEEEEECCEEEEEEEEECCCCCCCCCCCHHH
TMLADRLRDLNLRVDRLKTGTPPRLDARTINFDVLAKQHGDAELPVMSFMGSVDLHPRQI
HHHHHHHHHCCCEEEEECCCCCCCCCEEEEEEEEEEECCCCCCCCHHHHCCCCCCCCCCC
PCHITHTNEQTHDLIRNSLDRSPMYTGIIEGIGPRYCPSIEDKVMRFSDRNSHQIYLEPE
EEEEECCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEECCC
GLSTIEVYPNGISTSLPFDVQMGIVNSMKGLEKTRIIKPGYAIEYDYFDPRDLKPTLETK
CCEEEEEECCCCCCCCCCEEEHHHHHHHCCCCHHEEECCCCEEEECCCCCCCCCCCHHHH
AIEGLFFAGQINGTTGYEEAAAQGLLAGINAALQVQGKEAWFPTRDLAYTGVLVDDLCTL
HHCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHEEECHHHHHHHHC
GTKEPYRVFTSRAEYRLLLREDNADIRLTPIAHELGLIDDARWARFNQKMENIERERERL
CCCCCHHHHHCCCEEEEEEEECCCCEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KQIWIHPQSEHLAVVNELVNSPLTREASGEDLLRRPEVTYDKLTQVAAFAPALDDKQAAE
HHHHCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHC
QVEISIKYQGYIEHQQNEIERHKRHENTLIPAEFDYDKVESLSNEVRAKLMQHRPVSIGQ
EEEEEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
ASRISGITPAAISILLVNLKKQGMLKRGEL
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA