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The map label for this gene is dacA [H]

Identifier: 126209060

GI number: 126209060

Start: 1822752

End: 1823930

Strand: Reverse

Name: dacA [H]

Synonym: APL_1596

Alternate gene names: 126209060

Gene position: 1823930-1822752 (Counterclockwise)

Preceding gene: 126209061

Following gene: 126209059

Centisome position: 80.19

GC content: 42.66

Gene sequence:

>1179_bases
ATGAAAAGAACGTTATTAAAATCCGTAAGCCTTTGCGCTTTAGCCGTTTCTCAATTTGCGACCGCAGACGCAAATGTGGA
TTTCGGTATTTCCGCACCGCAATTAAATGCACAGACCTATATTTTAATGGACTATAATTCGGGTAATGTATTGGCAAGTT
TAAATCCGGATCAACGTCAATATCCGGCATCATTGACCAAAATGATGACCAGTTATGTAGTGGGGGACGCCCTTAAACAA
GGTAAAGTAAAAAACAGCGATATGGTGACCGTTACCGAGAATTCTTGGGCGCAAAAATTCCCGGGATCATCATTAATGTT
CTTGGATTTAAACACGCAAGTTTCGGTTGCCGATTTAATGCGCGGTTTAATTATCGTATCGGGTAATGATGCGGCGGTGG
CGTTGGCGGAACATACTTCCGGCTCGCAACAAGCCTTTATCGACCAAATGAACAAGTTCGGTCAGCAATTCGGTTTGAAA
AATACCAATTTTACGACCGTACACGGTTTGGATGATCCGAATCAATATTCATCTGCACGCGATATGGCGATTATCGGTGT
GCATGTTATTCGTGATCAGCCGGAAGAATATAAAATTTATGCCGAAAAAGAATTTAAATACAACATTAAAAAAGCACAGC
CGAACCGTAACGGTTTGCTTTGGGATAAAACGATTAATGTGGACGGTATGAAAACAGGTCACACCAGTCAAGCCGGTTAT
AATTTAGTCGCATCCGCAACGAATAACAACACCCGTTTAATTTCGGTGGTAATGGGCGTACCGACGTATAAAGGCCGTGA
AGTCGAAAGTAAAAAACTCCTGCAATGGGGTTTTGCAAACTTTGAAACGGTAAAATCGTTACAAGCGGGACAAGCGGTTT
CCGAACAGAGCGTTTACTACGGCGAAGCGGATAAAGTCCAACTCGGTTCAATTCAAGACAGCTTTGTGACCGTACCGAAA
GGTAAGGCGTCCGAATTAAAAGCTCGTTACGAGTTGGAACGTAAAAACCTTGAAGCGCCTTTAGCGAAAGGTCAGGTTAT
CGGTAAAGTGATTTACCAATTGGACGGTAAAGATGTTGCAAGTAGCGATCTGCAAGTGTTACAAGACGTACCTGAAGCGG
GTATTTTCGGTAAAGCATGGGATTGGGTGGTATTAACCGTTAAGAGTTTATTTGACTAA

Upstream 100 bases:

>100_bases
TTTAAATATTTTATATCTCGCATCAGGGTTTGTTGAGTTTCAACAAGCCCTTGTTTGCTTTATATCACCTGTTGTTTCCC
CCAAAGAATTGGATGCTTTT

Downstream 100 bases:

>100_bases
TCTCGGCTAACTTGTAATGTTTAAAATCGTCCCCATATTCGGACGAGTAATATACAAGCGGTATGATTTCGCAAAGTTCT
TGCAAGATCATACCGCTTAG

Product: D-alanyl-D-alanine carboxypeptidase fraction A

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKRTLLKSVSLCALAVSQFATADANVDFGISAPQLNAQTYILMDYNSGNVLASLNPDQRQYPASLTKMMTSYVVGDALKQ
GKVKNSDMVTVTENSWAQKFPGSSLMFLDLNTQVSVADLMRGLIIVSGNDAAVALAEHTSGSQQAFIDQMNKFGQQFGLK
NTNFTTVHGLDDPNQYSSARDMAIIGVHVIRDQPEEYKIYAEKEFKYNIKKAQPNRNGLLWDKTINVDGMKTGHTSQAGY
NLVASATNNNTRLISVVMGVPTYKGREVESKKLLQWGFANFETVKSLQAGQAVSEQSVYYGEADKVQLGSIQDSFVTVPK
GKASELKARYELERKNLEAPLAKGQVIGKVIYQLDGKDVASSDLQVLQDVPEAGIFGKAWDWVVLTVKSLFD

Sequences:

>Translated_392_residues
MKRTLLKSVSLCALAVSQFATADANVDFGISAPQLNAQTYILMDYNSGNVLASLNPDQRQYPASLTKMMTSYVVGDALKQ
GKVKNSDMVTVTENSWAQKFPGSSLMFLDLNTQVSVADLMRGLIIVSGNDAAVALAEHTSGSQQAFIDQMNKFGQQFGLK
NTNFTTVHGLDDPNQYSSARDMAIIGVHVIRDQPEEYKIYAEKEFKYNIKKAQPNRNGLLWDKTINVDGMKTGHTSQAGY
NLVASATNNNTRLISVVMGVPTYKGREVESKKLLQWGFANFETVKSLQAGQAVSEQSVYYGEADKVQLGSIQDSFVTVPK
GKASELKARYELERKNLEAPLAKGQVIGKVIYQLDGKDVASSDLQVLQDVPEAGIFGKAWDWVVLTVKSLFD
>Mature_392_residues
MKRTLLKSVSLCALAVSQFATADANVDFGISAPQLNAQTYILMDYNSGNVLASLNPDQRQYPASLTKMMTSYVVGDALKQ
GKVKNSDMVTVTENSWAQKFPGSSLMFLDLNTQVSVADLMRGLIIVSGNDAAVALAEHTSGSQQAFIDQMNKFGQQFGLK
NTNFTTVHGLDDPNQYSSARDMAIIGVHVIRDQPEEYKIYAEKEFKYNIKKAQPNRNGLLWDKTINVDGMKTGHTSQAGY
NLVASATNNNTRLISVVMGVPTYKGREVESKKLLQWGFANFETVKSLQAGQAVSEQSVYYGEADKVQLGSIQDSFVTVPK
GKASELKARYELERKNLEAPLAKGQVIGKVIYQLDGKDVASSDLQVLQDVPEAGIFGKAWDWVVLTVKSLFD

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=395, Percent_Identity=52.9113924050633, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1787062, Length=391, Percent_Identity=50.1278772378517, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI87082022, Length=386, Percent_Identity=45.0777202072539, Blast_Score=341, Evalue=4e-95,
Organism=Escherichia coli, GI87082054, Length=245, Percent_Identity=28.1632653061224, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43024; Mature: 43024

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTLLKSVSLCALAVSQFATADANVDFGISAPQLNAQTYILMDYNSGNVLASLNPDQRQ
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEECCCCHHH
YPASLTKMMTSYVVGDALKQGKVKNSDMVTVTENSWAQKFPGSSLMFLDLNTQVSVADLM
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCCEEEEEECCCCEEHHHHH
RGLIIVSGNDAAVALAEHTSGSQQAFIDQMNKFGQQFGLKNTNFTTVHGLDDPNQYSSAR
CCEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHCCCC
DMAIIGVHVIRDQPEEYKIYAEKEFKYNIKKAQPNRNGLLWDKTINVDGMKTGHTSQAGY
CEEEEEEEEECCCCCCEEEEEEEEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCC
NLVASATNNNTRLISVVMGVPTYKGREVESKKLLQWGFANFETVKSLQAGQAVSEQSVYY
EEEEECCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEE
GEADKVQLGSIQDSFVTVPKGKASELKARYELERKNLEAPLAKGQVIGKVIYQLDGKDVA
CCCCCEEECCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
SSDLQVLQDVPEAGIFGKAWDWVVLTVKSLFD
HHHHHHHHHCCCCCCCCCCCHHHEEEHHHHCC
>Mature Secondary Structure
MKRTLLKSVSLCALAVSQFATADANVDFGISAPQLNAQTYILMDYNSGNVLASLNPDQRQ
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEECCCCHHH
YPASLTKMMTSYVVGDALKQGKVKNSDMVTVTENSWAQKFPGSSLMFLDLNTQVSVADLM
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCCEEEEEECCCCEEHHHHH
RGLIIVSGNDAAVALAEHTSGSQQAFIDQMNKFGQQFGLKNTNFTTVHGLDDPNQYSSAR
CCEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHCCCC
DMAIIGVHVIRDQPEEYKIYAEKEFKYNIKKAQPNRNGLLWDKTINVDGMKTGHTSQAGY
CEEEEEEEEECCCCCCEEEEEEEEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCC
NLVASATNNNTRLISVVMGVPTYKGREVESKKLLQWGFANFETVKSLQAGQAVSEQSVYY
EEEEECCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEE
GEADKVQLGSIQDSFVTVPKGKASELKARYELERKNLEAPLAKGQVIGKVIYQLDGKDVA
CCCCCEEECCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
SSDLQVLQDVPEAGIFGKAWDWVVLTVKSLFD
HHHHHHHHHCCCCCCCCCCCHHHEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]