Click here to switch to the map view.

The map label for this gene is kdsB

Identifier: 126209041

GI number: 126209041

Start: 1803473

End: 1804231

Strand: Reverse

Name: kdsB

Synonym: APL_1577

Alternate gene names: 126209041

Gene position: 1804231-1803473 (Counterclockwise)

Preceding gene: 126209042

Following gene: 126209040

Centisome position: 79.32

GC content: 36.5

Gene sequence:

>759_bases
ATGAAGTTTACTATTATTATCCCGGCTCGTTATGCTTCGACTCGCTTACCTAGAAAACCGTTATTGGATATTTTAGGTAA
GCCGATGATCCAACACGTTTGGGAAAGAGCAAAGCAAGCGGGAGGACATCGAGTAATTATTGCAACAGATCATTCTGAGA
TTGCTGAGGTTGTTACTCGCTTTGGTGGAGAAGTTTGCCTAACTTCGGATAAACATTCATCCGGAACAGAACGTTTAGCT
GAAGTTGTTAGTAAAATGAATATTAGTGATGATGAGATTATTGTTAATGTTCAAGGCGATGAGCCTCTCATTCCACCGTG
TATTATCAAACAAGTTGCAGAGAATTTAGATAATCATCAAGTAAATATGGCAACATTAGCGGTTAAATTAACTCAAAGAG
ATGAATTATTTAATCCTAATGTAGTAAAAGTACTTTCAGATAAAAATGGTATGGCGTTATATTTTTCTCGTGCAGCTATT
CCATTTGCTAGAGATAACTTCCCTGATTGTTCAGATGATTTTGTTACTCAAAACCAGTATCTTCGTCATATAGGTATTTA
TGCTTATCGTGCAGGCTTCATTAAGCAGTATGTACAATGGCAACCGACGGCTTTAGAACAGCTAGAATCTTTAGAGCAGT
TAAGGGCTTTGTGGAATGGAGAAAAGATACACTTAGATATTGCATTAGAAACGCCTGAAGTTGGTGTTGATACGCAAGAA
GATTTAGAGCGAGTTCGTTTAATTTTATCAAATAAATAA

Upstream 100 bases:

>100_bases
GAAAGCGATTGATGATTTAGTTAAATCATTTGAAGAAATTGATACATCAAGATAAGAAATACTTCCCCTCTTAATTAGAG
GGGATTTTTATGAGGTTTTT

Downstream 100 bases:

>100_bases
GGCTATAAAATATGAATTACTTAGCAAGTGCTAGAGAAACATTATCTTTATATACTCAAGCAATAGATAGTTTACATAAT
CGCTTATCTACTGAGTTTAA

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase 2; CKS 2; CMP-KDO synthase 2

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKFTIIIPARYASTRLPRKPLLDILGKPMIQHVWERAKQAGGHRVIIATDHSEIAEVVTRFGGEVCLTSDKHSSGTERLA
EVVSKMNISDDEIIVNVQGDEPLIPPCIIKQVAENLDNHQVNMATLAVKLTQRDELFNPNVVKVLSDKNGMALYFSRAAI
PFARDNFPDCSDDFVTQNQYLRHIGIYAYRAGFIKQYVQWQPTALEQLESLEQLRALWNGEKIHLDIALETPEVGVDTQE
DLERVRLILSNK

Sequences:

>Translated_252_residues
MKFTIIIPARYASTRLPRKPLLDILGKPMIQHVWERAKQAGGHRVIIATDHSEIAEVVTRFGGEVCLTSDKHSSGTERLA
EVVSKMNISDDEIIVNVQGDEPLIPPCIIKQVAENLDNHQVNMATLAVKLTQRDELFNPNVVKVLSDKNGMALYFSRAAI
PFARDNFPDCSDDFVTQNQYLRHIGIYAYRAGFIKQYVQWQPTALEQLESLEQLRALWNGEKIHLDIALETPEVGVDTQE
DLERVRLILSNK
>Mature_252_residues
MKFTIIIPARYASTRLPRKPLLDILGKPMIQHVWERAKQAGGHRVIIATDHSEIAEVVTRFGGEVCLTSDKHSSGTERLA
EVVSKMNISDDEIIVNVQGDEPLIPPCIIKQVAENLDNHQVNMATLAVKLTQRDELFNPNVVKVLSDKNGMALYFSRAAI
PFARDNFPDCSDDFVTQNQYLRHIGIYAYRAGFIKQYVQWQPTALEQLESLEQLRALWNGEKIHLDIALETPEVGVDTQE
DLERVRLILSNK

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family

Homologues:

Organism=Escherichia coli, GI1787147, Length=246, Percent_Identity=62.1951219512195, Blast_Score=318, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDSB2_ACTP2 (A3N2M5)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054266.1
- ProteinModelPortal:   A3N2M5
- SMR:   A3N2M5
- STRING:   A3N2M5
- GeneID:   4850210
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1577
- eggNOG:   COG1212
- HOGENOM:   HBG637773
- OMA:   RFTHENG
- PhylomeDB:   A3N2M5
- ProtClustDB:   PRK05450
- BioCyc:   APLE416269:APL_1577-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00057
- InterPro:   IPR003329
- InterPro:   IPR004528
- TIGRFAMs:   TIGR00466

Pfam domain/function: PF02348 CTP_transf_3

EC number: =2.7.7.38

Molecular weight: Translated: 28530; Mature: 28530

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTIIIPARYASTRLPRKPLLDILGKPMIQHVWERAKQAGGHRVIIATDHSEIAEVVTR
CEEEEEEECHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHH
FGGEVCLTSDKHSSGTERLAEVVSKMNISDDEIIVNVQGDEPLIPPCIIKQVAENLDNHQ
CCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCC
VNMATLAVKLTQRDELFNPNVVKVLSDKNGMALYFSRAAIPFARDNFPDCSDDFVTQNQY
EEEEHEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
LRHIGIYAYRAGFIKQYVQWQPTALEQLESLEQLRALWNGEKIHLDIALETPEVGVDTQE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHH
DLERVRLILSNK
HHHHHHHHHCCC
>Mature Secondary Structure
MKFTIIIPARYASTRLPRKPLLDILGKPMIQHVWERAKQAGGHRVIIATDHSEIAEVVTR
CEEEEEEECHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHH
FGGEVCLTSDKHSSGTERLAEVVSKMNISDDEIIVNVQGDEPLIPPCIIKQVAENLDNHQ
CCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCC
VNMATLAVKLTQRDELFNPNVVKVLSDKNGMALYFSRAAIPFARDNFPDCSDDFVTQNQY
EEEEHEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
LRHIGIYAYRAGFIKQYVQWQPTALEQLESLEQLRALWNGEKIHLDIALETPEVGVDTQE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHH
DLERVRLILSNK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA