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The map label for this gene is dcuB [H]

Identifier: 126208996

GI number: 126208996

Start: 1746506

End: 1747828

Strand: Direct

Name: dcuB [H]

Synonym: APL_1532

Alternate gene names: 126208996

Gene position: 1746506-1747828 (Clockwise)

Preceding gene: 126208994

Following gene: 126208999

Centisome position: 76.79

GC content: 43.99

Gene sequence:

>1323_bases
ATGTCCGCAATGTTTCTTTTGCAATTTGCCATAGTTTTACTATGTATTTTAATCGGTGCCCGGGTGGGTGGTATCGGTTT
GGGTGTATTCGGTGGTTTAGGTCTTGCAATTCTTTCTTTCGGCTTCGGTTTAAAACCGGCGGGTCTGCCGATCGACGTAA
TGTTTATGATTATGGCGGTCGTATCTGCAGCGGCGGCAATGCAAGCTGCCGGCGGTTTAGACTATATGATTAAAATCGCT
ACTAACATTTTACGTCGTAATCCGAAATATATTACTTTTATCGCTCCGCTCGTTACTTGGACATTCACCGTGTTAGCCGG
TACCGGTCACGTAGCATACTCGGTATTACCGGTTATCGCTGAAGTAAGCCGTCACAACGGCATTCGTCCTGAACGTCCGC
TTTCAATGGCGGTAATCGCTTCGCAATTCGCTATCGTTGCCAGCCCTATCGCCGCAGCGGTGGTTGCAGTAGTAGCCTTC
TTAGAACCGCAAGGCATTACATTAGGTAACGTACTTTCGGTCACCATGCCTGCGACATTACTCGGTTTAATGCTTGCCTG
CGTATTCGTTAATAAAATGGGTAAAGAATTAAAAGACGATCCGCATTACCAAAAATTATTACAAGATCCGGAATATGTAA
AAGCAAATAACACAAATACCAATCTTGCGGATATCGTAATCAAACCGACGGCAAAACTTTCCGTTAGCTTATTCTTATTC
GGTGCATTGTTAGTCGTATTAATGGGTGCGGTGCCTTCATTACGCCCGGTATTCGACGGTAAACCGATGGGTATGGCGCA
TACGATTGAAATCGTTATGCTTACAATCGGTGCGTTAATCATCTTCTTCTGTAAACCGGACGGTAACGAGATTACAAAAG
GTTCGGTATTCCATGCCGGTATGCGTGCGGTAATCGCCATCTTCGGTATCGCATGGTTAGGCGATACCTTAATGCAAGGT
CATATGACAGAAGTGAAAGGTATGGTATCAGGTTTAGTAGAAACTGCGCCATGGGCATTTGCTTTCGCACTGTTTATTCT
ATCCGTATTAGTAAACAGCCAAGGGGCAACCGTAGCGACACTTTTCCCATTAGCTATCGCATTACAAATCGATCCGACAG
TACTTATTGCGGTATTCGTAGCGGTAAACGGTTATTTCTTTATTCCTAACTATGGTCCGATTATCGCTTCAATCGACTTC
GATACGACCGGTACCACTCGAATCGGTAAATATATCTTTAACCACAGCTTTATGTTACCGGGCTTATTAAGCATGGCGTT
TAGTATTGCCTTCGGTTTATTATTAGCCAATATTCTACTTTAA

Upstream 100 bases:

>100_bases
TCAAAGCATTCGTTCCGCTTGAGAAGGTTTCTAATTTCTGAAAAGGTTTTGCCTCAAGTATGATGGCTTTTTTGATGAAT
TACAATGGAGTAATTAAATT

Downstream 100 bases:

>100_bases
TTCGCTGATGATTTAAGAAAATACAAATGCCCTCCAACGTTAAGTTGCGAGGGCATTTTTTTGCAAAAAATTTAGGAAAT
CCGACCGCTTGTAAAAAGGC

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSAMFLLQFAIVLLCILIGARVGGIGLGVFGGLGLAILSFGFGLKPAGLPIDVMFMIMAVVSAAAAMQAAGGLDYMIKIA
TNILRRNPKYITFIAPLVTWTFTVLAGTGHVAYSVLPVIAEVSRHNGIRPERPLSMAVIASQFAIVASPIAAAVVAVVAF
LEPQGITLGNVLSVTMPATLLGLMLACVFVNKMGKELKDDPHYQKLLQDPEYVKANNTNTNLADIVIKPTAKLSVSLFLF
GALLVVLMGAVPSLRPVFDGKPMGMAHTIEIVMLTIGALIIFFCKPDGNEITKGSVFHAGMRAVIAIFGIAWLGDTLMQG
HMTEVKGMVSGLVETAPWAFAFALFILSVLVNSQGATVATLFPLAIALQIDPTVLIAVFVAVNGYFFIPNYGPIIASIDF
DTTGTTRIGKYIFNHSFMLPGLLSMAFSIAFGLLLANILL

Sequences:

>Translated_440_residues
MSAMFLLQFAIVLLCILIGARVGGIGLGVFGGLGLAILSFGFGLKPAGLPIDVMFMIMAVVSAAAAMQAAGGLDYMIKIA
TNILRRNPKYITFIAPLVTWTFTVLAGTGHVAYSVLPVIAEVSRHNGIRPERPLSMAVIASQFAIVASPIAAAVVAVVAF
LEPQGITLGNVLSVTMPATLLGLMLACVFVNKMGKELKDDPHYQKLLQDPEYVKANNTNTNLADIVIKPTAKLSVSLFLF
GALLVVLMGAVPSLRPVFDGKPMGMAHTIEIVMLTIGALIIFFCKPDGNEITKGSVFHAGMRAVIAIFGIAWLGDTLMQG
HMTEVKGMVSGLVETAPWAFAFALFILSVLVNSQGATVATLFPLAIALQIDPTVLIAVFVAVNGYFFIPNYGPIIASIDF
DTTGTTRIGKYIFNHSFMLPGLLSMAFSIAFGLLLANILL
>Mature_439_residues
SAMFLLQFAIVLLCILIGARVGGIGLGVFGGLGLAILSFGFGLKPAGLPIDVMFMIMAVVSAAAAMQAAGGLDYMIKIAT
NILRRNPKYITFIAPLVTWTFTVLAGTGHVAYSVLPVIAEVSRHNGIRPERPLSMAVIASQFAIVASPIAAAVVAVVAFL
EPQGITLGNVLSVTMPATLLGLMLACVFVNKMGKELKDDPHYQKLLQDPEYVKANNTNTNLADIVIKPTAKLSVSLFLFG
ALLVVLMGAVPSLRPVFDGKPMGMAHTIEIVMLTIGALIIFFCKPDGNEITKGSVFHAGMRAVIAIFGIAWLGDTLMQGH
MTEVKGMVSGLVETAPWAFAFALFILSVLVNSQGATVATLFPLAIALQIDPTVLIAVFVAVNGYFFIPNYGPIIASIDFD
TTGTTRIGKYIFNHSFMLPGLLSMAFSIAFGLLLANILL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=439, Percent_Identity=43.2801822323462, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1790565, Length=403, Percent_Identity=42.1836228287841, Blast_Score=323, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 46638; Mature: 46506

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAMFLLQFAIVLLCILIGARVGGIGLGVFGGLGLAILSFGFGLKPAGLPIDVMFMIMAV
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
VSAAAAMQAAGGLDYMIKIATNILRRNPKYITFIAPLVTWTFTVLAGTGHVAYSVLPVIA
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHH
EVSRHNGIRPERPLSMAVIASQFAIVASPIAAAVVAVVAFLEPQGITLGNVLSVTMPATL
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LGLMLACVFVNKMGKELKDDPHYQKLLQDPEYVKANNTNTNLADIVIKPTAKLSVSLFLF
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEECCCCCCCEEEEEEECCHHHHHHHHHH
GALLVVLMGAVPSLRPVFDGKPMGMAHTIEIVMLTIGALIIFFCKPDGNEITKGSVFHAG
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHH
MRAVIAIFGIAWLGDTLMQGHMTEVKGMVSGLVETAPWAFAFALFILSVLVNSQGATVAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHH
LFPLAIALQIDPTVLIAVFVAVNGYFFIPNYGPIIASIDFDTTGTTRIGKYIFNHSFMLP
HHHHHHEEECCHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHH
GLLSMAFSIAFGLLLANILL
HHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SAMFLLQFAIVLLCILIGARVGGIGLGVFGGLGLAILSFGFGLKPAGLPIDVMFMIMAV
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
VSAAAAMQAAGGLDYMIKIATNILRRNPKYITFIAPLVTWTFTVLAGTGHVAYSVLPVIA
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHH
EVSRHNGIRPERPLSMAVIASQFAIVASPIAAAVVAVVAFLEPQGITLGNVLSVTMPATL
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LGLMLACVFVNKMGKELKDDPHYQKLLQDPEYVKANNTNTNLADIVIKPTAKLSVSLFLF
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEECCCCCCCEEEEEEECCHHHHHHHHHH
GALLVVLMGAVPSLRPVFDGKPMGMAHTIEIVMLTIGALIIFFCKPDGNEITKGSVFHAG
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHH
MRAVIAIFGIAWLGDTLMQGHMTEVKGMVSGLVETAPWAFAFALFILSVLVNSQGATVAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHH
LFPLAIALQIDPTVLIAVFVAVNGYFFIPNYGPIIASIDFDTTGTTRIGKYIFNHSFMLP
HHHHHHEEECCHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHH
GLLSMAFSIAFGLLLANILL
HHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]