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The map label for this gene is poxA [H]

Identifier: 126208994

GI number: 126208994

Start: 1744436

End: 1745419

Strand: Direct

Name: poxA [H]

Synonym: APL_1530

Alternate gene names: 126208994

Gene position: 1744436-1745419 (Clockwise)

Preceding gene: 126208985

Following gene: 126208996

Centisome position: 76.7

GC content: 46.24

Gene sequence:

>984_bases
ATGAACGAATTAACTTTGGAAAATATCGAGTGGAAACCGACCGCTTCCATTCAGAACCTGATCAAACGCAGCAAAATTAT
GGCGGAAATTCGCCAGTTTTTTAAAGATCGCGGCGTATTAGAAGTGGAAACCCCGGCATTAAGCGAGTTTTCCGTAACGG
ACGTGCATCTTTCTACCTTCAGCACCCAATTTCTCTCGCCATTTTCCAGCGAAGCGAAAACATTGCATCTGATGACTAGC
CCCGAATACCATATGAAACGCTTATTGGCGGCGGGTAGCGGTGCAATTTTCCAATTATGTCGCGTATTTCGTAATGAAGA
AGCCGGTAAACGTCATAATCCGGAATTTACTATGCTGGAATGGTATCGCCCGCACTTTGATATGTATCGTTTAATCAATG
AAGTGGATGACTTATTACAGCAAATTTTGGATTGCGAACCGGCGGAATCTTATAGCTATCAGTTCGTGTTCCAAACTTAC
GTCGGTTTAGATCCGCTTTCGGCAACTCGAGCGCAATTGGTTGAAAAAGCCCGTAAACACGGCTTCCCGTGTGAAGATGA
CGAGAATCGAGATACTTTGTTGCAATTCCTGTTCAGTGAAATTGTGGAAGCGAATATCGGTAAAGAGCGCCCGACTGCGG
TCTATCATTTCCCGTCGTCGCAAGCGGCACTGGCGCAAATCAGCTCGGAAGATAACCGTGTTGCGGAACGCTTCGAGGTT
TATTACCAAGGCTTAGAGTTGGCAAACGGTTTTCATGAACTTAGTGATGCGAAAGAGCAAATGCGCCGTTTTGAGCAAGA
CAACGTACAGCGTGCGCAAATGGGCTTACCGGCGCAACAGTTGGACTCTCGTTTTCTTGCCGCATTAAAAGCGGGCATTC
CGAATTGTTCCGGCGTTGCGTTAGGTGTGGATCGCTTAATGATGATTGCGATGAATGCCGAGAAAATCGATGAAGTAATG
GCATTCTGTGTGGAAAACGCTTAG

Upstream 100 bases:

>100_bases
TTATGGAAAACGATATGAAATTTCTTGTAGCGAAAATTAGCTTTCGCAAAATAAAGCAAATCTCGTTAAAATAAATTTTT
TATGTCTAAAAAGCATTTTT

Downstream 100 bases:

>100_bases
GAGCGATTAGCCATAAATAACAAGCGGTCAAATTCTTGCAAAATTTTGCAGAAATTTGACCGCTTACTTTTAGCTAAAAA
CTTAAAGTTTAATAAACCCG

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNELTLENIEWKPTASIQNLIKRSKIMAEIRQFFKDRGVLEVETPALSEFSVTDVHLSTFSTQFLSPFSSEAKTLHLMTS
PEYHMKRLLAAGSGAIFQLCRVFRNEEAGKRHNPEFTMLEWYRPHFDMYRLINEVDDLLQQILDCEPAESYSYQFVFQTY
VGLDPLSATRAQLVEKARKHGFPCEDDENRDTLLQFLFSEIVEANIGKERPTAVYHFPSSQAALAQISSEDNRVAERFEV
YYQGLELANGFHELSDAKEQMRRFEQDNVQRAQMGLPAQQLDSRFLAALKAGIPNCSGVALGVDRLMMIAMNAEKIDEVM
AFCVENA

Sequences:

>Translated_327_residues
MNELTLENIEWKPTASIQNLIKRSKIMAEIRQFFKDRGVLEVETPALSEFSVTDVHLSTFSTQFLSPFSSEAKTLHLMTS
PEYHMKRLLAAGSGAIFQLCRVFRNEEAGKRHNPEFTMLEWYRPHFDMYRLINEVDDLLQQILDCEPAESYSYQFVFQTY
VGLDPLSATRAQLVEKARKHGFPCEDDENRDTLLQFLFSEIVEANIGKERPTAVYHFPSSQAALAQISSEDNRVAERFEV
YYQGLELANGFHELSDAKEQMRRFEQDNVQRAQMGLPAQQLDSRFLAALKAGIPNCSGVALGVDRLMMIAMNAEKIDEVM
AFCVENA
>Mature_327_residues
MNELTLENIEWKPTASIQNLIKRSKIMAEIRQFFKDRGVLEVETPALSEFSVTDVHLSTFSTQFLSPFSSEAKTLHLMTS
PEYHMKRLLAAGSGAIFQLCRVFRNEEAGKRHNPEFTMLEWYRPHFDMYRLINEVDDLLQQILDCEPAESYSYQFVFQTY
VGLDPLSATRAQLVEKARKHGFPCEDDENRDTLLQFLFSEIVEANIGKERPTAVYHFPSSQAALAQISSEDNRVAERFEV
YYQGLELANGFHELSDAKEQMRRFEQDNVQRAQMGLPAQQLDSRFLAALKAGIPNCSGVALGVDRLMMIAMNAEKIDEVM
AFCVENA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=337, Percent_Identity=25.8160237388724, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI5031815, Length=337, Percent_Identity=25.8160237388724, Blast_Score=111, Evalue=9e-25,
Organism=Escherichia coli, GI87082379, Length=323, Percent_Identity=65.3250773993808, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1790571, Length=330, Percent_Identity=29.6969696969697, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789256, Length=323, Percent_Identity=28.7925696594427, Blast_Score=137, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI71994340, Length=336, Percent_Identity=28.2738095238095, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17535927, Length=336, Percent_Identity=28.2738095238095, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17535925, Length=336, Percent_Identity=28.2738095238095, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324256, Length=359, Percent_Identity=26.4623955431755, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320242, Length=335, Percent_Identity=26.5671641791045, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24640851, Length=332, Percent_Identity=25.3012048192771, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24640849, Length=332, Percent_Identity=25.3012048192771, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 37402; Mature: 37402

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELTLENIEWKPTASIQNLIKRSKIMAEIRQFFKDRGVLEVETPALSEFSVTDVHLSTF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHH
STQFLSPFSSEAKTLHLMTSPEYHMKRLLAAGSGAIFQLCRVFRNEEAGKRHNPEFTMLE
HHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEHH
WYRPHFDMYRLINEVDDLLQQILDCEPAESYSYQFVFQTYVGLDPLSATRAQLVEKARKH
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHC
GFPCEDDENRDTLLQFLFSEIVEANIGKERPTAVYHFPSSQAALAQISSEDNRVAERFEV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHH
YYQGLELANGFHELSDAKEQMRRFEQDNVQRAQMGLPAQQLDSRFLAALKAGIPNCSGVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHH
LGVDRLMMIAMNAEKIDEVMAFCVENA
HHHHHHHHHHHCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNELTLENIEWKPTASIQNLIKRSKIMAEIRQFFKDRGVLEVETPALSEFSVTDVHLSTF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHH
STQFLSPFSSEAKTLHLMTSPEYHMKRLLAAGSGAIFQLCRVFRNEEAGKRHNPEFTMLE
HHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEHH
WYRPHFDMYRLINEVDDLLQQILDCEPAESYSYQFVFQTYVGLDPLSATRAQLVEKARKH
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHC
GFPCEDDENRDTLLQFLFSEIVEANIGKERPTAVYHFPSSQAALAQISSEDNRVAERFEV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHCCCHHHHHHHHHH
YYQGLELANGFHELSDAKEQMRRFEQDNVQRAQMGLPAQQLDSRFLAALKAGIPNCSGVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHH
LGVDRLMMIAMNAEKIDEVMAFCVENA
HHHHHHHHHHHCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA