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The map label for this gene is tig

Identifier: 126208971

GI number: 126208971

Start: 1723353

End: 1724654

Strand: Direct

Name: tig

Synonym: APL_1507

Alternate gene names: 126208971

Gene position: 1723353-1724654 (Clockwise)

Preceding gene: 126208970

Following gene: 126208972

Centisome position: 75.77

GC content: 42.01

Gene sequence:

>1302_bases
ATGTCAATTTCTATTGAAACTTTAGAAGGCTTACAACGCCGTGTAACGATTACCGTAGCTGCAGACAAAATCGAAGCGGC
TTATAAAGAACAATTAAAAGGCTATGCGAAAAACGCTCGTGTAGACGGTTTCCGTAAAGGTAAAGTGCCGCACGCAATTA
TCGAACAACGTTTCGGTTTAGCGGCTCGCCAAGACGTATTATCCGATGAAATGCAACGTGCGTTCTTTGATGCGGTAATC
GCAGAGAAAATCAACCTTGCAGGTCGTCCTACCTTCACACCGAACAACTACCAACCGGGTCAAGAATTCAGCTTCACAGC
AACGTTTGAAGTATTCCCGGAAGTTGAATTAAAAGGCTTAGAAAATATCGAAGTTGAAAAACCGGTTGTAGAAATCACAG
AAGCTGATTTAGACAAAATGATCGATGTGTTACGTAAACAACAAGCGACTTGGGCTGAATCTCAAGCAGCGGCGCAAGCG
GAAGACCGTGTTGTAATCGACTTCGTCGGTTCTGTTGACGGTGAAGAGTTTGAAGGCGGTAAAGCGACAGACTTCACTTT
AGCAATGGGTCAAGATCGTATGATCCCTGGTTTCGAAGAAGGTATCGTTGGTCACAAAGCCGGCGAACAATTCGATATCG
ATGTTACTTTCCCTGAAGAATACCACGCTGAAAACTTAAAAGGTAAAGCGGCGAAATTCGCAATTACACTTAAGAAAGTA
GAAAATATCGTATTACCTGAATTAACCGAAGAATTCGTGAAAAAATTCGGTTCAGCAAAAACTGTAGAAGATTTACGTGC
GGAAATTAAGAAAAATATGCAACGTGAACTTAAAAACGCAGTAACCGCACGCGTTAAAAACCAAGTAATCAACGGTTTAA
TCGCACAAAATGAAATTGAAGTGCCGGCTGCAGCGGTAGCGGAAGAAGTTGATGTATTACGTCGTCAAGCGGTTCAACGT
TTCGGTGGTAAACCGGAAATGGCTGCACAATTACCGGCAGAATTATTCGAAGCTGATGCAAAACGTCGTGTTCAAGTAGG
TTTATTACTTTCAACCGTAATCGGTACCAACGAATTAAAAGTTGATGAAAAACGTGTTGAAGAAACGATTGCAGAAATCG
CTTCAGCTTACGAACAACCGGCGGAAGTTGTTGCTCATTATGCGAAAAACCGTCAATTAACCGAAAATATCCGTAACGTA
GTGTTAGAAGAGCAAGCGGTTGAAGTTGTACTTGCGAAAGCAAAAGTAACTGAAAAAGCGACTTCTTTTGATGAAGTAAT
GGCTCAACAAGCTCAAGGCTAA

Upstream 100 bases:

>100_bases
TTGTTCGGAATCCTATTATCCCTCGACAGATCATTGACATAACGTTACAATGCACGTCTATTTCTGTATCTTTTATATAC
CCCCAACTTGAGGTTTAAGA

Downstream 100 bases:

>100_bases
TTCTCAAACATAAAGCTCTCCGGTTTGGAGGGTTTTTAATTAGCATTTTATAAGCGGTTGATTTTAGGAAAAAATTTGCA
AAAATTTCTCTAAAAATGAC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSISIETLEGLQRRVTITVAADKIEAAYKEQLKGYAKNARVDGFRKGKVPHAIIEQRFGLAARQDVLSDEMQRAFFDAVI
AEKINLAGRPTFTPNNYQPGQEFSFTATFEVFPEVELKGLENIEVEKPVVEITEADLDKMIDVLRKQQATWAESQAAAQA
EDRVVIDFVGSVDGEEFEGGKATDFTLAMGQDRMIPGFEEGIVGHKAGEQFDIDVTFPEEYHAENLKGKAAKFAITLKKV
ENIVLPELTEEFVKKFGSAKTVEDLRAEIKKNMQRELKNAVTARVKNQVINGLIAQNEIEVPAAAVAEEVDVLRRQAVQR
FGGKPEMAAQLPAELFEADAKRRVQVGLLLSTVIGTNELKVDEKRVEETIAEIASAYEQPAEVVAHYAKNRQLTENIRNV
VLEEQAVEVVLAKAKVTEKATSFDEVMAQQAQG

Sequences:

>Translated_433_residues
MSISIETLEGLQRRVTITVAADKIEAAYKEQLKGYAKNARVDGFRKGKVPHAIIEQRFGLAARQDVLSDEMQRAFFDAVI
AEKINLAGRPTFTPNNYQPGQEFSFTATFEVFPEVELKGLENIEVEKPVVEITEADLDKMIDVLRKQQATWAESQAAAQA
EDRVVIDFVGSVDGEEFEGGKATDFTLAMGQDRMIPGFEEGIVGHKAGEQFDIDVTFPEEYHAENLKGKAAKFAITLKKV
ENIVLPELTEEFVKKFGSAKTVEDLRAEIKKNMQRELKNAVTARVKNQVINGLIAQNEIEVPAAAVAEEVDVLRRQAVQR
FGGKPEMAAQLPAELFEADAKRRVQVGLLLSTVIGTNELKVDEKRVEETIAEIASAYEQPAEVVAHYAKNRQLTENIRNV
VLEEQAVEVVLAKAKVTEKATSFDEVMAQQAQG
>Mature_432_residues
SISIETLEGLQRRVTITVAADKIEAAYKEQLKGYAKNARVDGFRKGKVPHAIIEQRFGLAARQDVLSDEMQRAFFDAVIA
EKINLAGRPTFTPNNYQPGQEFSFTATFEVFPEVELKGLENIEVEKPVVEITEADLDKMIDVLRKQQATWAESQAAAQAE
DRVVIDFVGSVDGEEFEGGKATDFTLAMGQDRMIPGFEEGIVGHKAGEQFDIDVTFPEEYHAENLKGKAAKFAITLKKVE
NIVLPELTEEFVKKFGSAKTVEDLRAEIKKNMQRELKNAVTARVKNQVINGLIAQNEIEVPAAAVAEEVDVLRRQAVQRF
GGKPEMAAQLPAELFEADAKRRVQVGLLLSTVIGTNELKVDEKRVEETIAEIASAYEQPAEVVAHYAKNRQLTENIRNVV
LEEQAVEVVLAKAKVTEKATSFDEVMAQQAQG

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=432, Percent_Identity=66.4351851851852, Blast_Score=582, Evalue=1e-167,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_ACTP2 (A3N2F5)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054196.1
- ProteinModelPortal:   A3N2F5
- SMR:   A3N2F5
- STRING:   A3N2F5
- GeneID:   4849723
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1507
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   A3N2F5
- ProtClustDB:   PRK01490
- BioCyc:   APLE416269:APL_1507-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 48022; Mature: 47891

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISIETLEGLQRRVTITVAADKIEAAYKEQLKGYAKNARVDGFRKGKVPHAIIEQRFGL
CCCCHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCH
AARQDVLSDEMQRAFFDAVIAEKINLAGRPTFTPNNYQPGQEFSFTATFEVFPEVELKGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHCCC
ENIEVEKPVVEITEADLDKMIDVLRKQQATWAESQAAAQAEDRVVIDFVGSVDGEEFEGG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
KATDFTLAMGQDRMIPGFEEGIVGHKAGEQFDIDVTFPEEYHAENLKGKAAKFAITLKKV
CCCEEEEECCCCCCCCCHHHCCCCCCCCCEEEEEEECCHHHHHHCCCCCHHHHEEEHHHH
ENIVLPELTEEFVKKFGSAKTVEDLRAEIKKNMQRELKNAVTARVKNQVINGLIAQNEIE
HHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VPAAAVAEEVDVLRRQAVQRFGGKPEMAAQLPAELFEADAKRRVQVGLLLSTVIGTNELK
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VDEKRVEETIAEIASAYEQPAEVVAHYAKNRQLTENIRNVVLEEQAVEVVLAKAKVTEKA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSFDEVMAQQAQG
HHHHHHHHHHCCC
>Mature Secondary Structure 
SISIETLEGLQRRVTITVAADKIEAAYKEQLKGYAKNARVDGFRKGKVPHAIIEQRFGL
CCCHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCH
AARQDVLSDEMQRAFFDAVIAEKINLAGRPTFTPNNYQPGQEFSFTATFEVFPEVELKGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHCCC
ENIEVEKPVVEITEADLDKMIDVLRKQQATWAESQAAAQAEDRVVIDFVGSVDGEEFEGG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
KATDFTLAMGQDRMIPGFEEGIVGHKAGEQFDIDVTFPEEYHAENLKGKAAKFAITLKKV
CCCEEEEECCCCCCCCCHHHCCCCCCCCCEEEEEEECCHHHHHHCCCCCHHHHEEEHHHH
ENIVLPELTEEFVKKFGSAKTVEDLRAEIKKNMQRELKNAVTARVKNQVINGLIAQNEIE
HHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VPAAAVAEEVDVLRRQAVQRFGGKPEMAAQLPAELFEADAKRRVQVGLLLSTVIGTNELK
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VDEKRVEETIAEIASAYEQPAEVVAHYAKNRQLTENIRNVVLEEQAVEVVLAKAKVTEKA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSFDEVMAQQAQG
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA