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The map label for this gene is tnaB [H]

Identifier: 126208874

GI number: 126208874

Start: 1612209

End: 1613411

Strand: Direct

Name: tnaB [H]

Synonym: APL_1410

Alternate gene names: 126208874

Gene position: 1612209-1613411 (Clockwise)

Preceding gene: 126208872

Following gene: 126208876

Centisome position: 70.88

GC content: 43.06

Gene sequence:

>1203_bases
ATGAGAAAACAGCCAACACTTTTTGGTGGCGCTTGTATCATTGCAGGTGTCTGCGTAGGAGCAGGTATGCTTGGACTCCC
GACTTCCGGTGCGGGAGCTTGGACAATGTGGTCTATTCTTGCACTGGCTTTCACCATGATTGTGATGACATTTTCCGGCT
GGTTACTACTTGACGTCTATAAAAACTACGACCTACGTGCGTCTTTCAATACCGTTACCAAAGACTTATTAGGTAATAAA
ATTAATGCGTTAAATAACCTTGCAGTCTATTTTGTCGGTGGGATTTTACTTTACGCTTACACAACCGCTTCCGGTGGGAT
TTTAGAAAATCTCACCAAATCGGTGATTGATTTCGGTGAATTCGGTTCACGTATTTGGTCGGTGCTATTCGTTCTGATTT
TCTCGTTCTTTGTATGGCGCTCTACTCGCTTGGTCGATCGCATTTCGGTGCTATTAATTATCTTTATGGCACTTTCCTTT
GCGTTCAGTATCTCGGGTCTGGTCAGTAATATTGATAGCAATATTTTATTCGATCAGCAAAATGAGAGCGGAGAATACGC
CAAATACGCATTAGCGATGCTGCCGGTTGCACTAACCTCTTTCGGCTATCACCACTCAGTCTCTTCAATGCGTGCTTATT
ACGGTGAAGTGAAAAAAGCAAAATATGCGATTGCAGGCGGTACTTTTATTGCCTTAGTGCTTTATTTACTGTGGGTGATC
AGCATTTTCGGCAATTTACCGCGTGCGCAATTTGCACCGGTGATTGCAAGTAACGGTGATTTAGACGTGTTACTCAATAC
GATTGGTGAAGTAGTGCAGTCGCCGTATGTGAAACAAGCGATCAATGCCTTTTCTATGGCAGCGATTCTGTCTTCTTTTA
TCGGAGTTGGGCTAGGTACGTTTGACTTCTTGGCGGATTTCTTCAAATTTGACAACAGCAAATTAGGCAGGAGCAAAGCA
TGGGCGGTGACTTTCTTACCTCCACTAGTATTCTCATTACTTTCACCGCTCGGTTTCTTAAAAGCAATTGGTTACGCAGG
TGCGGTGGCAACCCTTTGGACTTGTATGATTCCGGCGCTATTAGCCTATAAAGCGAAAAAAGGTAATCTGGTTATGATTG
GATTGGTATTCTTATTCGGTGTTTGTACTGCGGTGTTCCATTTCCTTTCAATGTACGAAATGTTACCAATGTTTAAAGGC
TAA

Upstream 100 bases:

>100_bases
GATGATAAAAATACACTTTTTCTCTATAAGACTATTTTTTAATAAGTTAGTTTATGGCACAATACTATTACTTTAATGTA
CTACTTCAATAGGATATTTT

Downstream 100 bases:

>100_bases
CCCTTTATTTCTAACCACAGAAAACAAAAAATAAGGGGCAACCAATATTGCCCCTTTGTCTTTTTATCTGACCGCCATTT
GGAATAATACCACTTCCGCC

Product: ttryptophan-specific transport protein

Products: Indole [Cytoplasm]; L-tryptophan [Cytoplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Tryptophan permease [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MRKQPTLFGGACIIAGVCVGAGMLGLPTSGAGAWTMWSILALAFTMIVMTFSGWLLLDVYKNYDLRASFNTVTKDLLGNK
INALNNLAVYFVGGILLYAYTTASGGILENLTKSVIDFGEFGSRIWSVLFVLIFSFFVWRSTRLVDRISVLLIIFMALSF
AFSISGLVSNIDSNILFDQQNESGEYAKYALAMLPVALTSFGYHHSVSSMRAYYGEVKKAKYAIAGGTFIALVLYLLWVI
SIFGNLPRAQFAPVIASNGDLDVLLNTIGEVVQSPYVKQAINAFSMAAILSSFIGVGLGTFDFLADFFKFDNSKLGRSKA
WAVTFLPPLVFSLLSPLGFLKAIGYAGAVATLWTCMIPALLAYKAKKGNLVMIGLVFLFGVCTAVFHFLSMYEMLPMFKG

Sequences:

>Translated_400_residues
MRKQPTLFGGACIIAGVCVGAGMLGLPTSGAGAWTMWSILALAFTMIVMTFSGWLLLDVYKNYDLRASFNTVTKDLLGNK
INALNNLAVYFVGGILLYAYTTASGGILENLTKSVIDFGEFGSRIWSVLFVLIFSFFVWRSTRLVDRISVLLIIFMALSF
AFSISGLVSNIDSNILFDQQNESGEYAKYALAMLPVALTSFGYHHSVSSMRAYYGEVKKAKYAIAGGTFIALVLYLLWVI
SIFGNLPRAQFAPVIASNGDLDVLLNTIGEVVQSPYVKQAINAFSMAAILSSFIGVGLGTFDFLADFFKFDNSKLGRSKA
WAVTFLPPLVFSLLSPLGFLKAIGYAGAVATLWTCMIPALLAYKAKKGNLVMIGLVFLFGVCTAVFHFLSMYEMLPMFKG
>Mature_400_residues
MRKQPTLFGGACIIAGVCVGAGMLGLPTSGAGAWTMWSILALAFTMIVMTFSGWLLLDVYKNYDLRASFNTVTKDLLGNK
INALNNLAVYFVGGILLYAYTTASGGILENLTKSVIDFGEFGSRIWSVLFVLIFSFFVWRSTRLVDRISVLLIIFMALSF
AFSISGLVSNIDSNILFDQQNESGEYAKYALAMLPVALTSFGYHHSVSSMRAYYGEVKKAKYAIAGGTFIALVLYLLWVI
SIFGNLPRAQFAPVIASNGDLDVLLNTIGEVVQSPYVKQAINAFSMAAILSSFIGVGLGTFDFLADFFKFDNSKLGRSKA
WAVTFLPPLVFSLLSPLGFLKAIGYAGAVATLWTCMIPALLAYKAKKGNLVMIGLVFLFGVCTAVFHFLSMYEMLPMFKG

Specific function: Involved in transporting tryptophan across the cytoplasmic membrane [H]

COG id: COG0814

COG function: function code E; Amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid/polyamine transporter 2 family. Mtr/TnaB/TyrP permease subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789552, Length=392, Percent_Identity=39.2857142857143, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1790145, Length=411, Percent_Identity=36.7396593673966, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1788218, Length=372, Percent_Identity=33.3333333333333, Blast_Score=172, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002091
- InterPro:   IPR013061
- InterPro:   IPR013059
- InterPro:   IPR018227 [H]

Pfam domain/function: PF03222 Trp_Tyr_perm [H]

EC number: NA

Molecular weight: Translated: 43571; Mature: 43571

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKQPTLFGGACIIAGVCVGAGMLGLPTSGAGAWTMWSILALAFTMIVMTFSGWLLLDVY
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHEEHHH
KNYDLRASFNTVTKDLLGNKINALNNLAVYFVGGILLYAYTTASGGILENLTKSVIDFGE
HCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
FGSRIWSVLFVLIFSFFVWRSTRLVDRISVLLIIFMALSFAFSISGLVSNIDSNILFDQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
NESGEYAKYALAMLPVALTSFGYHHSVSSMRAYYGEVKKAKYAIAGGTFIALVLYLLWVI
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SIFGNLPRAQFAPVIASNGDLDVLLNTIGEVVQSPYVKQAINAFSMAAILSSFIGVGLGT
HHHCCCCCHHHCCHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHH
FDFLADFFKFDNSKLGRSKAWAVTFLPPLVFSLLSPLGFLKAIGYAGAVATLWTCMIPAL
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAYKAKKGNLVMIGLVFLFGVCTAVFHFLSMYEMLPMFKG
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKQPTLFGGACIIAGVCVGAGMLGLPTSGAGAWTMWSILALAFTMIVMTFSGWLLLDVY
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHEEHHH
KNYDLRASFNTVTKDLLGNKINALNNLAVYFVGGILLYAYTTASGGILENLTKSVIDFGE
HCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
FGSRIWSVLFVLIFSFFVWRSTRLVDRISVLLIIFMALSFAFSISGLVSNIDSNILFDQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
NESGEYAKYALAMLPVALTSFGYHHSVSSMRAYYGEVKKAKYAIAGGTFIALVLYLLWVI
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SIFGNLPRAQFAPVIASNGDLDVLLNTIGEVVQSPYVKQAINAFSMAAILSSFIGVGLGT
HHHCCCCCHHHCCHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHH
FDFLADFFKFDNSKLGRSKAWAVTFLPPLVFSLLSPLGFLKAIGYAGAVATLWTCMIPAL
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAYKAKKGNLVMIGLVFLFGVCTAVFHFLSMYEMLPMFKG
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Indole [Periplasm]; L-tryptophan [Periplasm]; Proton [Periplasm] [C]

Specific reaction: Proton [Periplasm] + indole [Periplasm] = Proton [Cytoplasm] + indole [Cytoplasm] L-tryptophan [Periplasm] + Proton [Periplasm] = L-tryptophan [Cytoplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]