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The map label for this gene is mltF

Identifier: 126208830

GI number: 126208830

Start: 1562283

End: 1563650

Strand: Reverse

Name: mltF

Synonym: APL_1366

Alternate gene names: 126208830

Gene position: 1563650-1562283 (Counterclockwise)

Preceding gene: 126208831

Following gene: 126208825

Centisome position: 68.75

GC content: 42.11

Gene sequence:

>1368_bases
ATGCAGGCGGAAGAAAATCGCTATAACCAAATTCAACAACGTAAAATATTAAGAGTCGGAATGGTTAATCATCCTCTTTC
CTATTTTATCGGGGCGGAAGGTACCGCCGGGATAGAATACGAATTGGCCAAATCTTTTGCTAATTATCTTGACGTAAGAT
TGGATATTAAAACTTTTGATAACAGTGAGCAGCTCTTTAGCGCATTAAAAGATAATAAAGTGGATATTGCGGCGGCAGGC
TTATTATACCAGCCGGAATTGAGTAAGCAGTTTCAAATCGGTTCGGCTTATTATTCCGCTTCTTGGCAAGTCGTATATAA
AAAAGGCAGTAACCGCCCTTATAAACTGTCGGAATTAGAAGGTGATTTGATCATACCTGCCGGTTCAGCGGTATTGCCGA
TTTTACAACGCTTAAAAGAAGATAATCCGAAACTAAGTTGGCAAACTACCAATCAGTTTACGCAGGAAGAGTTATTGCTC
CAAGTCGCCGAGGGAAAAATTCCTTATACGGTCGGCGTATCCGTTGATATTTCCGCCGCACAACATATCCGCCCGAATAT
TGCGGTCGGTTTCGATTTAACCGATGAGGCGCCGGTATTGTGGTATTTACCGAACAGTTCTTATAGTGAGCTGCAAGCTG
CGGTATTGGATTTTATGAATCATGCGAATGAAACCGGATTGATCTCACGGATTGAAGAAAAATATTTTAATCATTTGGCG
CATTTTGATTACGTCGATATCCAAAGTTATTTAAAAGCAATTAAATTGGTCTTACCTAAATATCAGTCCCTGTTTGAAAA
GTATTGCGGCGATTTGGAATGGCAGATGCTGGCGGCTATCGCTTATCAGGAATCGCATTGGGATCCGAATGCGACTTCTC
CGACCGGTGTACGCGGTATGATGATGCTGACCAGAGATACGGCAGAGCGTATGAAAATTACGGATCGAACTAGTGCGGAA
CAAAGTATTCGTGCCGGTTCCGAATATCTTCATATGCTGATGAGACAAATTCCCGAAACGGTACCGAAAGAAGATCGTAT
TTGGTACGGATTAGCCGCTTATAATATGGGACTCGGGCATTTATTGGATGTTCGCCGTTTAACGAGGCAGTTGGGCGGTA
ATCCGGATAATTGGTTGGATGTGAAGAAGAACCTACCGCTTTTAGCGGAGAAGCGTCATTATAGCGGTTTAAAATACGGC
TATGCTCGAGGGTTTGAGGCATTTCAGTATGTCGAAAATATTCGTCGATATTATAGTAGTATCATTAACCATCAAAGGGT
AGAGGAACAACAAATCCAGAATAATGAAGAGCAACCGAGTGTTCCGCAAGAGATAAGCAAAGAATCGGATTCAACTTTGA
AGGAATAG

Upstream 100 bases:

>100_bases
TATAGAGGATTTTATATTGAAAGGATTAATTGCCAGATTCATTGCAGGCTTTGCGCTTTTGCTTTGGGCTTGGGATATGG
TATTTCCGTGGCAACAATTA

Downstream 100 bases:

>100_bases
AGCAATGAAACGTAAACTTAAAACCAAGAAAAAACTCGTGTGTAATCATAAAGCGCGTCGTCTTTTTCATATCTGGAAAA
CGAAGCACCGTATTGCGGAA

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MQAEENRYNQIQQRKILRVGMVNHPLSYFIGAEGTAGIEYELAKSFANYLDVRLDIKTFDNSEQLFSALKDNKVDIAAAG
LLYQPELSKQFQIGSAYYSASWQVVYKKGSNRPYKLSELEGDLIIPAGSAVLPILQRLKEDNPKLSWQTTNQFTQEELLL
QVAEGKIPYTVGVSVDISAAQHIRPNIAVGFDLTDEAPVLWYLPNSSYSELQAAVLDFMNHANETGLISRIEEKYFNHLA
HFDYVDIQSYLKAIKLVLPKYQSLFEKYCGDLEWQMLAAIAYQESHWDPNATSPTGVRGMMMLTRDTAERMKITDRTSAE
QSIRAGSEYLHMLMRQIPETVPKEDRIWYGLAAYNMGLGHLLDVRRLTRQLGGNPDNWLDVKKNLPLLAEKRHYSGLKYG
YARGFEAFQYVENIRRYYSSIINHQRVEEQQIQNNEEQPSVPQEISKESDSTLKE

Sequences:

>Translated_455_residues
MQAEENRYNQIQQRKILRVGMVNHPLSYFIGAEGTAGIEYELAKSFANYLDVRLDIKTFDNSEQLFSALKDNKVDIAAAG
LLYQPELSKQFQIGSAYYSASWQVVYKKGSNRPYKLSELEGDLIIPAGSAVLPILQRLKEDNPKLSWQTTNQFTQEELLL
QVAEGKIPYTVGVSVDISAAQHIRPNIAVGFDLTDEAPVLWYLPNSSYSELQAAVLDFMNHANETGLISRIEEKYFNHLA
HFDYVDIQSYLKAIKLVLPKYQSLFEKYCGDLEWQMLAAIAYQESHWDPNATSPTGVRGMMMLTRDTAERMKITDRTSAE
QSIRAGSEYLHMLMRQIPETVPKEDRIWYGLAAYNMGLGHLLDVRRLTRQLGGNPDNWLDVKKNLPLLAEKRHYSGLKYG
YARGFEAFQYVENIRRYYSSIINHQRVEEQQIQNNEEQPSVPQEISKESDSTLKE
>Mature_455_residues
MQAEENRYNQIQQRKILRVGMVNHPLSYFIGAEGTAGIEYELAKSFANYLDVRLDIKTFDNSEQLFSALKDNKVDIAAAG
LLYQPELSKQFQIGSAYYSASWQVVYKKGSNRPYKLSELEGDLIIPAGSAVLPILQRLKEDNPKLSWQTTNQFTQEELLL
QVAEGKIPYTVGVSVDISAAQHIRPNIAVGFDLTDEAPVLWYLPNSSYSELQAAVLDFMNHANETGLISRIEEKYFNHLA
HFDYVDIQSYLKAIKLVLPKYQSLFEKYCGDLEWQMLAAIAYQESHWDPNATSPTGVRGMMMLTRDTAERMKITDRTSAE
QSIRAGSEYLHMLMRQIPETVPKEDRIWYGLAAYNMGLGHLLDVRRLTRQLGGNPDNWLDVKKNLPLLAEKRHYSGLKYG
YARGFEAFQYVENIRRYYSSIINHQRVEEQQIQNNEEQPSVPQEISKESDSTLKE

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family

Homologues:

Organism=Escherichia coli, GI171474010, Length=452, Percent_Identity=49.3362831858407, Blast_Score=436, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLTF_ACTP2 (A3N214)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001054055.1
- ProteinModelPortal:   A3N214
- STRING:   A3N214
- GeneID:   4849619
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1366
- eggNOG:   COG4623
- HOGENOM:   HBG644469
- ProtClustDB:   PRK10859
- BioCyc:   APLE416269:APL_1366-MONOMER
- HAMAP:   MF_02016
- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT

EC number: NA

Molecular weight: Translated: 52149; Mature: 52149

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: ACT_SITE 312-312

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAEENRYNQIQQRKILRVGMVNHPLSYFIGAEGTAGIEYELAKSFANYLDVRLDIKTFD
CCCCCHHHHHHHHHHHHHHHCCCCCHHHEECCCCCCCCHHHHHHHHHHHHEEEEEEEECC
NSEQLFSALKDNKVDIAAAGLLYQPELSKQFQIGSAYYSASWQVVYKKGSNRPYKLSELE
CHHHHHHHHHCCCEEEEEECEEECCCCCCCEECCCCEECCCEEEEEECCCCCCEEHHCCC
GDLIIPAGSAVLPILQRLKEDNPKLSWQTTNQFTQEELLLQVAEGKIPYTVGVSVDISAA
CCEEEECCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEEECHH
QHIRPNIAVGFDLTDEAPVLWYLPNSSYSELQAAVLDFMNHANETGLISRIEEKYFNHLA
HHCCCCEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
HFDYVDIQSYLKAIKLVLPKYQSLFEKYCGDLEWQMLAAIAYQESHWDPNATSPTGVRGM
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEE
MMLTRDTAERMKITDRTSAEQSIRAGSEYLHMLMRQIPETVPKEDRIWYGLAAYNMGLGH
EEEEHHHHHHHEECCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCHHH
LLDVRRLTRQLGGNPDNWLDVKKNLPLLAEKRHYSGLKYGYARGFEAFQYVENIRRYYSS
HHHHHHHHHHHCCCCCCCEECCCCCCCEECHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IINHQRVEEQQIQNNEEQPSVPQEISKESDSTLKE
HHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MQAEENRYNQIQQRKILRVGMVNHPLSYFIGAEGTAGIEYELAKSFANYLDVRLDIKTFD
CCCCCHHHHHHHHHHHHHHHCCCCCHHHEECCCCCCCCHHHHHHHHHHHHEEEEEEEECC
NSEQLFSALKDNKVDIAAAGLLYQPELSKQFQIGSAYYSASWQVVYKKGSNRPYKLSELE
CHHHHHHHHHCCCEEEEEECEEECCCCCCCEECCCCEECCCEEEEEECCCCCCEEHHCCC
GDLIIPAGSAVLPILQRLKEDNPKLSWQTTNQFTQEELLLQVAEGKIPYTVGVSVDISAA
CCEEEECCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEEECHH
QHIRPNIAVGFDLTDEAPVLWYLPNSSYSELQAAVLDFMNHANETGLISRIEEKYFNHLA
HHCCCCEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
HFDYVDIQSYLKAIKLVLPKYQSLFEKYCGDLEWQMLAAIAYQESHWDPNATSPTGVRGM
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEE
MMLTRDTAERMKITDRTSAEQSIRAGSEYLHMLMRQIPETVPKEDRIWYGLAAYNMGLGH
EEEEHHHHHHHEECCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCHHH
LLDVRRLTRQLGGNPDNWLDVKKNLPLLAEKRHYSGLKYGYARGFEAFQYVENIRRYYSS
HHHHHHHHHHHCCCCCCCEECCCCCCCEECHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IINHQRVEEQQIQNNEEQPSVPQEISKESDSTLKE
HHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA