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The map label for this gene is rsmD [H]

Identifier: 126208811

GI number: 126208811

Start: 1547312

End: 1547905

Strand: Direct

Name: rsmD [H]

Synonym: APL_1347

Alternate gene names: 126208811

Gene position: 1547312-1547905 (Clockwise)

Preceding gene: 126208807

Following gene: 126208812

Centisome position: 68.03

GC content: 44.11

Gene sequence:

>594_bases
ATGAAAAAAAATCGTAATTCCACTTTACAAAACAAGCCGACCGGCGAAGTGCGCGTGATTGCCGGACTGTGGCGAGGGCG
AAAGCTGCCGGTGTTAAATACGGAAGGTTTACGCCCGACCACCGACCGAGTAAAAGAAACGCTGTTTAATTGGCTAATGA
ATGATGTAGCAGGCAGTCGATGTTTAGATTGTTTTGCGGGCAGTGGTTCTCTTGGGATTGAAGCGCTCTCTCGCCAAGCA
CAAGCGGTTGTTTTTCTTGAGAAATTTGCAAATGCGGCAAATCTGCTGAAAAATAATTTAACCTCGCTTAAAACTACAAA
TGGCACCGTAATTAATACCGATACGCTGCAATATTTAGCCCAAAAGAACTCGCAGCCAGGATTTGAAGTGGTATTTGTCG
ATCCGCCCTTTAATCACGGCTTTGTACCGAAGGTATTAAAGCTCTTAGAAGAAAACCAGTGGCTTGCCGACAATGCGTTG
ATTTATGTGGAAACGGAAAAGAACCATCCGCCGCTTGAATTACCGAGCAATTGGCAAATTCTTAAAGAAAAAACCGCCGG
ACAAGTAATAAGTCGCTTGATCCAATGTCTATAA

Upstream 100 bases:

>100_bases
AAACCTTTTTTCTTTTCTGCCATATTTTTCTCACTTTTCCTTCAAAATGGACTAAACTTGTATAGTTTATCAAATAATCC
TTATTTTTCCTTTAGTTAGT

Downstream 100 bases:

>100_bases
ATTTGACTTTCTAAACCTTTATTGGCAAATTAAGTCGCTATTTTTTTATATAAAAGAAACAAATTATGAACAAATTAACT
CCGGATGAAGCGATTGATCT

Product: putative methylase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MKKNRNSTLQNKPTGEVRVIAGLWRGRKLPVLNTEGLRPTTDRVKETLFNWLMNDVAGSRCLDCFAGSGSLGIEALSRQA
QAVVFLEKFANAANLLKNNLTSLKTTNGTVINTDTLQYLAQKNSQPGFEVVFVDPPFNHGFVPKVLKLLEENQWLADNAL
IYVETEKNHPPLELPSNWQILKEKTAGQVISRLIQCL

Sequences:

>Translated_197_residues
MKKNRNSTLQNKPTGEVRVIAGLWRGRKLPVLNTEGLRPTTDRVKETLFNWLMNDVAGSRCLDCFAGSGSLGIEALSRQA
QAVVFLEKFANAANLLKNNLTSLKTTNGTVINTDTLQYLAQKNSQPGFEVVFVDPPFNHGFVPKVLKLLEENQWLADNAL
IYVETEKNHPPLELPSNWQILKEKTAGQVISRLIQCL
>Mature_197_residues
MKKNRNSTLQNKPTGEVRVIAGLWRGRKLPVLNTEGLRPTTDRVKETLFNWLMNDVAGSRCLDCFAGSGSLGIEALSRQA
QAVVFLEKFANAANLLKNNLTSLKTTNGTVINTDTLQYLAQKNSQPGFEVVFVDPPFNHGFVPKVLKLLEENQWLADNAL
IYVETEKNHPPLELPSNWQILKEKTAGQVISRLIQCL

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=195, Percent_Identity=49.7435897435897, Blast_Score=196, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22002; Mature: 22002

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNRNSTLQNKPTGEVRVIAGLWRGRKLPVLNTEGLRPTTDRVKETLFNWLMNDVAGSR
CCCCCCCCCCCCCCCCEEEEEEHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCHHH
CLDCFAGSGSLGIEALSRQAQAVVFLEKFANAANLLKNNLTSLKTTNGTVINTDTLQYLA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEHHHHHHHH
QKNSQPGFEVVFVDPPFNHGFVPKVLKLLEENQWLADNALIYVETEKNHPPLELPSNWQI
HCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEECCCEEEEEEECCCCCCCCCCCCHHH
LKEKTAGQVISRLIQCL
HHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKKNRNSTLQNKPTGEVRVIAGLWRGRKLPVLNTEGLRPTTDRVKETLFNWLMNDVAGSR
CCCCCCCCCCCCCCCCEEEEEEHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCHHH
CLDCFAGSGSLGIEALSRQAQAVVFLEKFANAANLLKNNLTSLKTTNGTVINTDTLQYLA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEHHHHHHHH
QKNSQPGFEVVFVDPPFNHGFVPKVLKLLEENQWLADNALIYVETEKNHPPLELPSNWQI
HCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEECCCEEEEEEECCCCCCCCCCCCHHH
LKEKTAGQVISRLIQCL
HHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]