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The map label for this gene is dcuB2 [H]

Identifier: 126208780

GI number: 126208780

Start: 1506732

End: 1508066

Strand: Reverse

Name: dcuB2 [H]

Synonym: APL_1316

Alternate gene names: 126208780

Gene position: 1508066-1506732 (Counterclockwise)

Preceding gene: 126208783

Following gene: 126208777

Centisome position: 66.3

GC content: 43.6

Gene sequence:

>1335_bases
ATGCTTTATGCAGAATTTTTGCTTTTATTAGCATTTCTCTATGCGGGTAGCCGTTACGGCGGTATCGGTTTAGGGGTTGT
TTCCGGTATCGGTTTATTTGTGGAAGTATTTTTCCTCGGTATGCCGCTTTCAAAAGCACCGATTGACGTAATGTTGGTCA
TTTTAGCCGTGGTGACTTGTGCGTCCGTACTTGAAGCGGCGGGCGGTTTGAAATTTATGTTACAAATCGCGGAACGTATC
TTACGTAGTAATCCGAAACGCATCACGCTACTTGGTCCTATCGTAACTTATGTAATGACTTTAATGTTAGGTACCGGTCA
CTCGGTATATTCAATTATGCCGATTATCGGTGACATTGCGTTAAAAAATAAAATTCGTCCTGAACGCCCGATGGCAGCGG
CATCGGTTGCTTCGCAATTAGGGATTACCGGTAGCCCGCTTTCAGCTGCAGTGGCGTATTACTTAACCGAAATTACCAAA
TTACCGGGCTTTGAAGGCGTTACACTTTTAAATATCGTAGCGGTAACCATGTCTGCAACATTTGTTGGTGTACTTGCAAT
GTGTTTATATAGTTTACGTCGCGGTAAAGAGCTTGAGGATGATCCGGAATATCAACGTCGTATGCAAGACCCCGTATTGC
GTAAACAAATTGAAGAAACCAGTTCTACTTCATTAGACGAGCAATTACCGGCAAGTGCGAAAAATTCCGTTTATCTATTC
TTAGCGGCAATCGCAACGATCGTTATTATCGCAATGGTGCCGGCAATTAAACCGGTGATGGCGGATACGGGCAAAACGGC
CGGTATGGATAAAATCATTCAAATCGTGATGCTGACTTTCGGCGGTTTAATCTTAGTATTAACCAAAACCGATGTGAAAA
AAGTACCGAACGGCGTAGTATTTAAATCCGGTATGGTGGCGACTATCGCAATCTTCGGTATTGCGTGGATGAGCGATACT
TATTTCAGTTATGCGATGCCTTCATTTAAAGCGGGTGTAACCGAAATGATTAAAGCGCAGCCTTGGACGTTCGCCTTCGC
ATTATTTGCGGTGTCCGTAGTGGTAAACAGCCAAGCGGTAACGGCGAAAATGTTATTACCGGTCGGTATTGCCATCGGTT
TACCGGCACCGTTATTAGTCGGTTTAATGCCTGCAACCTATGCGTACTTCTTTATTCCTAACTATCCGTCAGATATCGCA
ACGGTAAACTTTGATGTGACAGGTACAACCAAAATCGGTAAATACTACTTTAACCACAGCTTTATGGCACCGGGTTTAGT
CGGGGTAATTACCGCATGTTGTGTGGGTATTGCATTAGCAAATATTCTGATTTAA

Upstream 100 bases:

>100_bases
AAAGTGTTAATCTGTTTTTGATCAAATGCAAAGAATCACGGCACTCGCTCAAGTACACTTGCGCACAGTGATTAGTTATT
TTTAATTTTTTGGAGTTTAT

Downstream 100 bases:

>100_bases
TGACTCAATAAAGTAAGCGGTTCGATTTAGTAAAATTTATACAAAATCGAACCGCTTATTTTTTGCGTTATAAACCTAAT
TGATTGAGTTTATCCGCTTC

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MLYAEFLLLLAFLYAGSRYGGIGLGVVSGIGLFVEVFFLGMPLSKAPIDVMLVILAVVTCASVLEAAGGLKFMLQIAERI
LRSNPKRITLLGPIVTYVMTLMLGTGHSVYSIMPIIGDIALKNKIRPERPMAAASVASQLGITGSPLSAAVAYYLTEITK
LPGFEGVTLLNIVAVTMSATFVGVLAMCLYSLRRGKELEDDPEYQRRMQDPVLRKQIEETSSTSLDEQLPASAKNSVYLF
LAAIATIVIIAMVPAIKPVMADTGKTAGMDKIIQIVMLTFGGLILVLTKTDVKKVPNGVVFKSGMVATIAIFGIAWMSDT
YFSYAMPSFKAGVTEMIKAQPWTFAFALFAVSVVVNSQAVTAKMLLPVGIAIGLPAPLLVGLMPATYAYFFIPNYPSDIA
TVNFDVTGTTKIGKYYFNHSFMAPGLVGVITACCVGIALANILI

Sequences:

>Translated_444_residues
MLYAEFLLLLAFLYAGSRYGGIGLGVVSGIGLFVEVFFLGMPLSKAPIDVMLVILAVVTCASVLEAAGGLKFMLQIAERI
LRSNPKRITLLGPIVTYVMTLMLGTGHSVYSIMPIIGDIALKNKIRPERPMAAASVASQLGITGSPLSAAVAYYLTEITK
LPGFEGVTLLNIVAVTMSATFVGVLAMCLYSLRRGKELEDDPEYQRRMQDPVLRKQIEETSSTSLDEQLPASAKNSVYLF
LAAIATIVIIAMVPAIKPVMADTGKTAGMDKIIQIVMLTFGGLILVLTKTDVKKVPNGVVFKSGMVATIAIFGIAWMSDT
YFSYAMPSFKAGVTEMIKAQPWTFAFALFAVSVVVNSQAVTAKMLLPVGIAIGLPAPLLVGLMPATYAYFFIPNYPSDIA
TVNFDVTGTTKIGKYYFNHSFMAPGLVGVITACCVGIALANILI
>Mature_444_residues
MLYAEFLLLLAFLYAGSRYGGIGLGVVSGIGLFVEVFFLGMPLSKAPIDVMLVILAVVTCASVLEAAGGLKFMLQIAERI
LRSNPKRITLLGPIVTYVMTLMLGTGHSVYSIMPIIGDIALKNKIRPERPMAAASVASQLGITGSPLSAAVAYYLTEITK
LPGFEGVTLLNIVAVTMSATFVGVLAMCLYSLRRGKELEDDPEYQRRMQDPVLRKQIEETSSTSLDEQLPASAKNSVYLF
LAAIATIVIIAMVPAIKPVMADTGKTAGMDKIIQIVMLTFGGLILVLTKTDVKKVPNGVVFKSGMVATIAIFGIAWMSDT
YFSYAMPSFKAGVTEMIKAQPWTFAFALFAVSVVVNSQAVTAKMLLPVGIAIGLPAPLLVGLMPATYAYFFIPNYPSDIA
TVNFDVTGTTKIGKYYFNHSFMAPGLVGVITACCVGIALANILI

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=406, Percent_Identity=42.8571428571429, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1790580, Length=455, Percent_Identity=35.6043956043956, Blast_Score=260, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 47466; Mature: 47466

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYAEFLLLLAFLYAGSRYGGIGLGVVSGIGLFVEVFFLGMPLSKAPIDVMLVILAVVTC
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
ASVLEAAGGLKFMLQIAERILRSNPKRITLLGPIVTYVMTLMLGTGHSVYSIMPIIGDIA
HHHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LKNKIRPERPMAAASVASQLGITGSPLSAAVAYYLTEITKLPGFEGVTLLNIVAVTMSAT
HHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
FVGVLAMCLYSLRRGKELEDDPEYQRRMQDPVLRKQIEETSSTSLDEQLPASAKNSVYLF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCHHHHCCCCCCCCHHHH
LAAIATIVIIAMVPAIKPVMADTGKTAGMDKIIQIVMLTFGGLILVLTKTDVKKVPNGVV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEECHHHHHCCCCEE
FKSGMVATIAIFGIAWMSDTYFSYAMPSFKAGVTEMIKAQPWTFAFALFAVSVVVNSQAV
ECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHH
TAKMLLPVGIAIGLPAPLLVGLMPATYAYFFIPNYPSDIATVNFDVTGTTKIGKYYFNHS
HHHHHHHHHHHHCCCHHHHHHHHHHHHEEEECCCCCCCCEEEEEEECCCHHHHHHHHCCC
FMAPGLVGVITACCVGIALANILI
CCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLYAEFLLLLAFLYAGSRYGGIGLGVVSGIGLFVEVFFLGMPLSKAPIDVMLVILAVVTC
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
ASVLEAAGGLKFMLQIAERILRSNPKRITLLGPIVTYVMTLMLGTGHSVYSIMPIIGDIA
HHHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LKNKIRPERPMAAASVASQLGITGSPLSAAVAYYLTEITKLPGFEGVTLLNIVAVTMSAT
HHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
FVGVLAMCLYSLRRGKELEDDPEYQRRMQDPVLRKQIEETSSTSLDEQLPASAKNSVYLF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCHHHHCCCCCCCCHHHH
LAAIATIVIIAMVPAIKPVMADTGKTAGMDKIIQIVMLTFGGLILVLTKTDVKKVPNGVV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEECHHHHHCCCCEE
FKSGMVATIAIFGIAWMSDTYFSYAMPSFKAGVTEMIKAQPWTFAFALFAVSVVVNSQAV
ECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHH
TAKMLLPVGIAIGLPAPLLVGLMPATYAYFFIPNYPSDIATVNFDVTGTTKIGKYYFNHS
HHHHHHHHHHHHCCCHHHHHHHHHHHHEEEECCCCCCCCEEEEEEECCCHHHHHHHHCCC
FMAPGLVGVITACCVGIALANILI
CCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]