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The map label for this gene is ptsB [H]

Identifier: 126208783

GI number: 126208783

Start: 1511140

End: 1512561

Strand: Reverse

Name: ptsB [H]

Synonym: APL_1319

Alternate gene names: 126208783

Gene position: 1512561-1511140 (Counterclockwise)

Preceding gene: 126208784

Following gene: 126208780

Centisome position: 66.5

GC content: 45.78

Gene sequence:

>1422_bases
ATGAACTTTCCGAAAATTGCGGAGCAAGCGATCGAGAAACTCGGTGGCAAAAGCAACATCACAACGCTGGCGCACTGCGC
AACACGCTTACGTTTAACCCTTGCAGATGAGTCAAAAGTAGATAAACAAGCGATTGAGAATATCGAAGGCGTTAAAGGTC
AATTCTCAACCAGCGGTCAATACCAAATTATTTTCGGTTCCGGTACGGTAAACAAAGTACACGCTGAGATGCAAAAACAA
ATGGGCATCGGCGATATGAGCACTTCCGAAGTGGCGGCGGCAGGTGCGGCTAATCAACCGTTGTTACAACGCTTAGTAAA
AGGGTTAGCGGATATTTTCGTACCGATCATTCCTGCAATCGTGGCAGGCGGTTTGCTGATGGGTATCCATTCAATGCTGA
CTTCGGTCGGTTTCTTCTGGGAAGGCGAGTCGGTTGCAAGTAAATATCCGCAATTTGCGGATCTGATTGATTTTATCAAT
ACGATTGCAAACGCACCGTTTGTCTTCTTACCGGTGTTACTCGGTTTCTCCGCAACGCGTAAATTCGGTGGTAACCCGTT
CTTAGGTGCGGCACTCGGTATGTTATTAGTTCACCCTGCATTAGCAGACGGTTGGAATTATGCTAAAACCTTAATGGAAG
GTAACATCAAATACTGGAACGTACTTGGTTTTGAAATTGAAAAAGTCGGTTATCAAGGTACGGTAATCCCAACCATCGTT
TCTGCGTGGGTGTTAGCAACCCTTGAAAAAGGTTTCCGTAAATTTGTACCTTCTTATTTAGATAACCTTGTGACACCGAT
GATGTCTTTATTTATCGCAGGTGTGTTAGCTTTCACGGTAATCGGTCCGTTCGGCCGTGAAGCGGGTTCCGCTATTTCAG
CAGGTTTAACTTGGTTATATGACAGCTTAGGTTTTATCGGCGGCGCAATCTTCGGTACATTCTATGCACCAATCGTCATC
ACCGGTATGCACCAAACCTTTATTGCGGTAGAAACGCAATTATTAGCGGAAATGGCGAATACCGGCGGTACATTTATCTT
CCCAATTGCAGCAATGTCTAACATCGCACAAGGTGCGGCGTGTTTAGGGGTAGCGTTCGCAATGAAAGATGCGAAAGTAC
GCGGTTTAGCCGTGCCGTCCGGTATCTCTGCATTATTAGGTATTACGGAACCGGCAATGTTCGGTGTGAACTTACGTTAC
CGTCAAGCGTTCGTTGCGGCAATGGTTGGTTCAGGTTTAGCGTCTGCGTTTATCGCATTCTTTAACGTGAAAGCGATTGC
ATTAGGTGCGGCGGGTTTCTTAGGTGTGCCGTCAATCAAACCGGACAGCCTTGCAATGTACTCTGTAGGTATGGCAATTT
CATTTATCGTTGCGTTCTCAATCTCAGCGGTATGGGTGAAAAAAGCACAAGCTAAAAAATAA

Upstream 100 bases:

>100_bases
CAAAACTAAAACGTTTTAGCAGAATTCTCTGTTTTAGCTAAAACGTTTTAGTTAAAATATAATCATTAAATTTTTGATAT
AAAAAAATAGGTGGCAAACT

Downstream 100 bases:

>100_bases
TTTAGCGATAAAACAATAGAGAAAGCCCCTAAAGCGTAAGGTTTATGGGCTTTCGTTTATGCTACAAGCGGTTAAATTTG
TTCGATTTTTTGCAAATGGG

Product: PTS system sucrose-specific EIIBC component

Products: NA

Alternate protein names: EIIBC-Scr; EII-Scr; Sucrose-specific phosphotransferase enzyme IIB component; PTS system sucrose-specific EIIB component; Sucrose permease IIC component; PTS system sucrose-specific EIIC component [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNFPKIAEQAIEKLGGKSNITTLAHCATRLRLTLADESKVDKQAIENIEGVKGQFSTSGQYQIIFGSGTVNKVHAEMQKQ
MGIGDMSTSEVAAAGAANQPLLQRLVKGLADIFVPIIPAIVAGGLLMGIHSMLTSVGFFWEGESVASKYPQFADLIDFIN
TIANAPFVFLPVLLGFSATRKFGGNPFLGAALGMLLVHPALADGWNYAKTLMEGNIKYWNVLGFEIEKVGYQGTVIPTIV
SAWVLATLEKGFRKFVPSYLDNLVTPMMSLFIAGVLAFTVIGPFGREAGSAISAGLTWLYDSLGFIGGAIFGTFYAPIVI
TGMHQTFIAVETQLLAEMANTGGTFIFPIAAMSNIAQGAACLGVAFAMKDAKVRGLAVPSGISALLGITEPAMFGVNLRY
RQAFVAAMVGSGLASAFIAFFNVKAIALGAAGFLGVPSIKPDSLAMYSVGMAISFIVAFSISAVWVKKAQAKK

Sequences:

>Translated_473_residues
MNFPKIAEQAIEKLGGKSNITTLAHCATRLRLTLADESKVDKQAIENIEGVKGQFSTSGQYQIIFGSGTVNKVHAEMQKQ
MGIGDMSTSEVAAAGAANQPLLQRLVKGLADIFVPIIPAIVAGGLLMGIHSMLTSVGFFWEGESVASKYPQFADLIDFIN
TIANAPFVFLPVLLGFSATRKFGGNPFLGAALGMLLVHPALADGWNYAKTLMEGNIKYWNVLGFEIEKVGYQGTVIPTIV
SAWVLATLEKGFRKFVPSYLDNLVTPMMSLFIAGVLAFTVIGPFGREAGSAISAGLTWLYDSLGFIGGAIFGTFYAPIVI
TGMHQTFIAVETQLLAEMANTGGTFIFPIAAMSNIAQGAACLGVAFAMKDAKVRGLAVPSGISALLGITEPAMFGVNLRY
RQAFVAAMVGSGLASAFIAFFNVKAIALGAAGFLGVPSIKPDSLAMYSVGMAISFIVAFSISAVWVKKAQAKK
>Mature_473_residues
MNFPKIAEQAIEKLGGKSNITTLAHCATRLRLTLADESKVDKQAIENIEGVKGQFSTSGQYQIIFGSGTVNKVHAEMQKQ
MGIGDMSTSEVAAAGAANQPLLQRLVKGLADIFVPIIPAIVAGGLLMGIHSMLTSVGFFWEGESVASKYPQFADLIDFIN
TIANAPFVFLPVLLGFSATRKFGGNPFLGAALGMLLVHPALADGWNYAKTLMEGNIKYWNVLGFEIEKVGYQGTVIPTIV
SAWVLATLEKGFRKFVPSYLDNLVTPMMSLFIAGVLAFTVIGPFGREAGSAISAGLTWLYDSLGFIGGAIFGTFYAPIVI
TGMHQTFIAVETQLLAEMANTGGTFIFPIAAMSNIAQGAACLGVAFAMKDAKVRGLAVPSGISALLGITEPAMFGVNLRY
RQAFVAAMVGSGLASAFIAFFNVKAIALGAAGFLGVPSIKPDSLAMYSVGMAISFIVAFSISAVWVKKAQAKK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI2367362, Length=436, Percent_Identity=37.8440366972477, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI1790159, Length=425, Percent_Identity=29.1764705882353, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI48994906, Length=437, Percent_Identity=28.604118993135, Blast_Score=182, Evalue=6e-47,
Organism=Escherichia coli, GI1788769, Length=479, Percent_Identity=30.2713987473904, Blast_Score=165, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010973 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 49947; Mature: 49947

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFPKIAEQAIEKLGGKSNITTLAHCATRLRLTLADESKVDKQAIENIEGVKGQFSTSGQ
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEECCHHHHHHHHHHHHHCCCCCCCCCCC
YQIIFGSGTVNKVHAEMQKQMGIGDMSTSEVAAAGAANQPLLQRLVKGLADIFVPIIPAI
EEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VAGGLLMGIHSMLTSVGFFWEGESVASKYPQFADLIDFINTIANAPFVFLPVLLGFSATR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KFGGNPFLGAALGMLLVHPALADGWNYAKTLMEGNIKYWNVLGFEIEKVGYQGTVIPTIV
HCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECEEHHHCCCCCCHHHHHH
SAWVLATLEKGFRKFVPSYLDNLVTPMMSLFIAGVLAFTVIGPFGREAGSAISAGLTWLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
DSLGFIGGAIFGTFYAPIVITGMHQTFIAVETQLLAEMANTGGTFIFPIAAMSNIAQGAA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
CLGVAFAMKDAKVRGLAVPSGISALLGITEPAMFGVNLRYRQAFVAAMVGSGLASAFIAF
HHHHHHHHHCCCCCCEECCCHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
FNVKAIALGAAGFLGVPSIKPDSLAMYSVGMAISFIVAFSISAVWVKKAQAKK
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNFPKIAEQAIEKLGGKSNITTLAHCATRLRLTLADESKVDKQAIENIEGVKGQFSTSGQ
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEECCHHHHHHHHHHHHHCCCCCCCCCCC
YQIIFGSGTVNKVHAEMQKQMGIGDMSTSEVAAAGAANQPLLQRLVKGLADIFVPIIPAI
EEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VAGGLLMGIHSMLTSVGFFWEGESVASKYPQFADLIDFINTIANAPFVFLPVLLGFSATR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KFGGNPFLGAALGMLLVHPALADGWNYAKTLMEGNIKYWNVLGFEIEKVGYQGTVIPTIV
HCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECEEHHHCCCCCCHHHHHH
SAWVLATLEKGFRKFVPSYLDNLVTPMMSLFIAGVLAFTVIGPFGREAGSAISAGLTWLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
DSLGFIGGAIFGTFYAPIVITGMHQTFIAVETQLLAEMANTGGTFIFPIAAMSNIAQGAA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
CLGVAFAMKDAKVRGLAVPSGISALLGITEPAMFGVNLRYRQAFVAAMVGSGLASAFIAF
HHHHHHHHHCCCCCCEECCCHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
FNVKAIALGAAGFLGVPSIKPDSLAMYSVGMAISFIVAFSISAVWVKKAQAKK
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]