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The map label for this gene is pcaK [H]

Identifier: 126208773

GI number: 126208773

Start: 1499927

End: 1501249

Strand: Reverse

Name: pcaK [H]

Synonym: APL_1309

Alternate gene names: 126208773

Gene position: 1501249-1499927 (Counterclockwise)

Preceding gene: 126208774

Following gene: 126208772

Centisome position: 66.0

GC content: 47.09

Gene sequence:

>1323_bases
ATGACAATTCAAACGCCACAATATACGAATAATTTAAGGTTCGCGATTGATCACGGACGTATGGGCGTATATCAATGGGC
AATCGTAATTATGGCTGCCGTGATGAACTTTTTAGACGGTTTCGACGTATTGGCGATTGCCTTTACCGCCACCAATATTT
CTAAAGAGTTCGGTTTATCTAAAACTGAGTTCGGTGTGTTAATCAGTGCCGGTTTAGCCGGTATGACGATCGGTTCGCTT
TTCCTTGCGCCATTGGCGGATAAATTCGGTCGTCGTCCGTTATTATTACTTTCCGTAGCGTTATCGGCGATTGGTTTGTT
ACTTTCGGGATTAGCGACCACACCGTTTGTACTTGGTTTTTCACGTGTAATTACCGGTTTGGGAGTGGGCGGTATTTTAG
TCGGCACCAATGTGATCACTAGCGAATATTCGTCAAAAAAATGGCGTAGTTTTGCGATCAGCGTTTATGCGGCAGGCTTC
GGTATCGGTGCGATGATCGGCGGTATAATGGCGAAAGAGTTGCAAGCGACTTATAGCTGGCATGCGGTGTATTTTGCCGG
TGCGGCAATGACGGCGGTCGTGTGGGTTGTGTTATTTATTTGGTTACCGGAGTCGATTGACTTTTTAACCATGAAGCAAC
CGGCAAACGCCAAAGCACGCTTAAATCACATTGCGAAAAAAATCGGTTTTAACGGTGATTGGGGGCTACCGAAAAAAGTT
GAAGCGGTGAAAACCAAGCTGCCGATTTCTCAGCTGTTTAATGATAAATATCGCCGTTCAACCTTATTATTATGGCTTTC
GTTCTTTGCCATTATGTTCAGCTTCTATTTTATCAGCTCATGGACTCCGGCATTGCTCAAAGAAGCGGGAATGACGGTGG
AAGACAGTATCAATGTAGGCATGGCGATTTCGATCGGCGGTGCGGGCGGTTCGTTAATCTACGGTTTAATCGCCAGCCGT
TGGCAGGCAAGAGCGGTATTAATGCTGTTTACCGTATTATCGGCGATTGCGATTGTGGTGTTTATCCTTTCATCTTCGGC
ACTCGGCGTGGCGATGGCAATGGGAGTGGTTGTCGGGGCGTTAGTAAACGGCTGTATCAGCGGTTTATACACTATCAATC
CGGCGACTTACGATGCGGATATCCGTAATACCGGTGTGGGTTGGGCGATAGGTGCGGGGCGTGCAGGCTCGATTCTTGCG
CCGACAGTCGCAGGAATATTGCTTGACGGCGGTTTGGCGAAACAAGATTTATATATCGCCGTGGCGGGCGTGATGTTACT
ATCAACCCTAGCATTAGCCTTCAAACGTGAAAGTAAAATTTGA

Upstream 100 bases:

>100_bases
CTTAATATTTTTCAATGCCGTTATTGCAGATTTGCATTGTTCTTTGCAATTGTTTCATTTAGGATTCCTATTAAATGAAA
ATCTTTAATGAGGAATACCT

Downstream 100 bases:

>100_bases
TTGTAGGAATCTATTAAGTATTCATAGTGTTTCACGGACGCAAGCACTTTTCCTATAAATCATAAGCGGTCGAATTTGCA
AATTTTTCTGCAAATTCGAC

Product: putative permease

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MTIQTPQYTNNLRFAIDHGRMGVYQWAIVIMAAVMNFLDGFDVLAIAFTATNISKEFGLSKTEFGVLISAGLAGMTIGSL
FLAPLADKFGRRPLLLLSVALSAIGLLLSGLATTPFVLGFSRVITGLGVGGILVGTNVITSEYSSKKWRSFAISVYAAGF
GIGAMIGGIMAKELQATYSWHAVYFAGAAMTAVVWVVLFIWLPESIDFLTMKQPANAKARLNHIAKKIGFNGDWGLPKKV
EAVKTKLPISQLFNDKYRRSTLLLWLSFFAIMFSFYFISSWTPALLKEAGMTVEDSINVGMAISIGGAGGSLIYGLIASR
WQARAVLMLFTVLSAIAIVVFILSSSALGVAMAMGVVVGALVNGCISGLYTINPATYDADIRNTGVGWAIGAGRAGSILA
PTVAGILLDGGLAKQDLYIAVAGVMLLSTLALAFKRESKI

Sequences:

>Translated_440_residues
MTIQTPQYTNNLRFAIDHGRMGVYQWAIVIMAAVMNFLDGFDVLAIAFTATNISKEFGLSKTEFGVLISAGLAGMTIGSL
FLAPLADKFGRRPLLLLSVALSAIGLLLSGLATTPFVLGFSRVITGLGVGGILVGTNVITSEYSSKKWRSFAISVYAAGF
GIGAMIGGIMAKELQATYSWHAVYFAGAAMTAVVWVVLFIWLPESIDFLTMKQPANAKARLNHIAKKIGFNGDWGLPKKV
EAVKTKLPISQLFNDKYRRSTLLLWLSFFAIMFSFYFISSWTPALLKEAGMTVEDSINVGMAISIGGAGGSLIYGLIASR
WQARAVLMLFTVLSAIAIVVFILSSSALGVAMAMGVVVGALVNGCISGLYTINPATYDADIRNTGVGWAIGAGRAGSILA
PTVAGILLDGGLAKQDLYIAVAGVMLLSTLALAFKRESKI
>Mature_439_residues
TIQTPQYTNNLRFAIDHGRMGVYQWAIVIMAAVMNFLDGFDVLAIAFTATNISKEFGLSKTEFGVLISAGLAGMTIGSLF
LAPLADKFGRRPLLLLSVALSAIGLLLSGLATTPFVLGFSRVITGLGVGGILVGTNVITSEYSSKKWRSFAISVYAAGFG
IGAMIGGIMAKELQATYSWHAVYFAGAAMTAVVWVVLFIWLPESIDFLTMKQPANAKARLNHIAKKIGFNGDWGLPKKVE
AVKTKLPISQLFNDKYRRSTLLLWLSFFAIMFSFYFISSWTPALLKEAGMTVEDSINVGMAISIGGAGGSLIYGLIASRW
QARAVLMLFTVLSAIAIVVFILSSSALGVAMAMGVVVGALVNGCISGLYTINPATYDADIRNTGVGWAIGAGRAGSILAP
TVAGILLDGGLAKQDLYIAVAGVMLLSTLALAFKRESKI

Specific function: Could Be A Transporter For 3-Phenylpropionate (Hydrocinnamic Acid). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=438, Percent_Identity=23.2876712328767, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI7662270, Length=334, Percent_Identity=24.251497005988, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI213021148, Length=470, Percent_Identity=21.7021276595745, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI87081723, Length=407, Percent_Identity=29.7297297297297, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87082404, Length=382, Percent_Identity=22.2513089005236, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87082231, Length=443, Percent_Identity=23.0248306997743, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082159, Length=373, Percent_Identity=24.3967828418231, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787880, Length=187, Percent_Identity=27.807486631016, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=429, Percent_Identity=22.8438228438228, Blast_Score=95, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46718; Mature: 46587

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQTPQYTNNLRFAIDHGRMGVYQWAIVIMAAVMNFLDGFDVLAIAFTATNISKEFGLS
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
KTEFGVLISAGLAGMTIGSLFLAPLADKFGRRPLLLLSVALSAIGLLLSGLATTPFVLGF
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRVITGLGVGGILVGTNVITSEYSSKKWRSFAISVYAAGFGIGAMIGGIMAKELQATYSW
HHHHHHCCCCHHHCCCHHHHHCCCCHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHH
HAVYFAGAAMTAVVWVVLFIWLPESIDFLTMKQPANAKARLNHIAKKIGFNGDWGLPKKV
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHH
EAVKTKLPISQLFNDKYRRSTLLLWLSFFAIMFSFYFISSWTPALLKEAGMTVEDSINVG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEECCCCCC
MAISIGGAGGSLIYGLIASRWQARAVLMLFTVLSAIAIVVFILSSSALGVAMAMGVVVGA
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LVNGCISGLYTINPATYDADIRNTGVGWAIGAGRAGSILAPTVAGILLDGGLAKQDLYIA
HHHHHHHCCEEECCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
VAGVMLLSTLALAFKRESKI
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIQTPQYTNNLRFAIDHGRMGVYQWAIVIMAAVMNFLDGFDVLAIAFTATNISKEFGLS
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
KTEFGVLISAGLAGMTIGSLFLAPLADKFGRRPLLLLSVALSAIGLLLSGLATTPFVLGF
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRVITGLGVGGILVGTNVITSEYSSKKWRSFAISVYAAGFGIGAMIGGIMAKELQATYSW
HHHHHHCCCCHHHCCCHHHHHCCCCHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHH
HAVYFAGAAMTAVVWVVLFIWLPESIDFLTMKQPANAKARLNHIAKKIGFNGDWGLPKKV
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHH
EAVKTKLPISQLFNDKYRRSTLLLWLSFFAIMFSFYFISSWTPALLKEAGMTVEDSINVG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEECCCCCC
MAISIGGAGGSLIYGLIASRWQARAVLMLFTVLSAIAIVVFILSSSALGVAMAMGVVVGA
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LVNGCISGLYTINPATYDADIRNTGVGWAIGAGRAGSILAPTVAGILLDGGLAKQDLYIA
HHHHHHHCCEEECCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
VAGVMLLSTLALAFKRESKI
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8063101 [H]