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The map label for this gene is pspE [H]

Identifier: 126208751

GI number: 126208751

Start: 1476170

End: 1476538

Strand: Direct

Name: pspE [H]

Synonym: APL_1285

Alternate gene names: 126208751

Gene position: 1476170-1476538 (Clockwise)

Preceding gene: 126208750

Following gene: 126208759

Centisome position: 64.9

GC content: 41.73

Gene sequence:

>369_bases
ATGAATAAATTAACTTACCTTTTAGGCGCTTTAGTGTTAGCTACTCCCGTCCTAACGCAAGCGGCGGAAATGAAAGAGAC
GGAGCAAACAATGCAAAATGCACCGCTAAAAGCTAAAGGTATTTGGATTGACGTCCGTACTGCGGACGAATTTCAACAAG
GGCATTTAGACGGTTCGATTAATATTCCTTTCGAACAAATTGCGGATCATATTAGCAAAGTAAGCCCGAATAAAGACGAA
CCGGTACATCTGTATTGCCGTTCCGGACGTAGAGCGGAAACCGCATTACAAACGTTAAAACAACTCGGCTATAACAATGT
AACTAACCACGGAGGCTATCAGGAACTGATCGAAAAAGGTTATAAATAA

Upstream 100 bases:

>100_bases
GAAAGTGAGATTGCGGGCGCATTAGCAACCGCTATTGCGTTAAATGCAGGTGCGGCTTATACCTATTCTTTAAGAGCTTT
AGAAGCAGTGGCGGTTCAAG

Downstream 100 bases:

>100_bases
ATAGAAACTAGATTTCTTATTAATGATTATTACACACTTTTAAGTACAAAAATTAACCCAACAGATTTCTTTAATTTTTA
CTTTCTAAAATTCTAAAAGG

Product: hypothetical protein

Products: NA

Alternate protein names: TST; Phage shock protein E [H]

Number of amino acids: Translated: 122; Mature: 122

Protein sequence:

>122_residues
MNKLTYLLGALVLATPVLTQAAEMKETEQTMQNAPLKAKGIWIDVRTADEFQQGHLDGSINIPFEQIADHISKVSPNKDE
PVHLYCRSGRRAETALQTLKQLGYNNVTNHGGYQELIEKGYK

Sequences:

>Translated_122_residues
MNKLTYLLGALVLATPVLTQAAEMKETEQTMQNAPLKAKGIWIDVRTADEFQQGHLDGSINIPFEQIADHISKVSPNKDE
PVHLYCRSGRRAETALQTLKQLGYNNVTNHGGYQELIEKGYK
>Mature_122_residues
MNKLTYLLGALVLATPVLTQAAEMKETEQTMQNAPLKAKGIWIDVRTADEFQQGHLDGSINIPFEQIADHISKVSPNKDE
PVHLYCRSGRRAETALQTLKQLGYNNVTNHGGYQELIEKGYK

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspE catalyzes the sulfur-transfer reaction from thiosulfate to cyanide, to form sulfite and thiocyan

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1787567, Length=82, Percent_Identity=37.8048780487805, Blast_Score=79, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014323
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 13654; Mature: 13654

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLTYLLGALVLATPVLTQAAEMKETEQTMQNAPLKAKGIWIDVRTADEFQQGHLDGSI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCCCCCC
NIPFEQIADHISKVSPNKDEPVHLYCRSGRRAETALQTLKQLGYNNVTNHGGYQELIEKG
CCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHCC
YK
CC
>Mature Secondary Structure
MNKLTYLLGALVLATPVLTQAAEMKETEQTMQNAPLKAKGIWIDVRTADEFQQGHLDGSI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCCCCCC
NIPFEQIADHISKVSPNKDEPVHLYCRSGRRAETALQTLKQLGYNNVTNHGGYQELIEKG
CCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHCC
YK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1712397; 9097039; 9278503; 9868784 [H]