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The map label for this gene is phoB [H]

Identifier: 126208723

GI number: 126208723

Start: 1446864

End: 1447505

Strand: Reverse

Name: phoB [H]

Synonym: APL_1257

Alternate gene names: 126208723

Gene position: 1447505-1446864 (Counterclockwise)

Preceding gene: 126208724

Following gene: 126208722

Centisome position: 63.64

GC content: 39.56

Gene sequence:

>642_bases
ATGATCTCTCTGTTTTTATTACAGCAGAATTATCAAGTGCTCGAAGCCGAGGATTATCAAAGTGCGGTCAAAAAGCTGAG
CGAGAAACCGAAGTTAATTCTACTGGATTGGATGTTACCGGGGCGTTCGGGCATTCAGTTTATTCAATATTTAAAAAAAT
CGGAAGAAACCGCACAAATTCCTATCTTAATGCTCACCGCTCGCAGTTCGGAAGACGATTGCATTGTTTGTCTTAATACC
GGTGCGGACGATTATGTGACTAAACCGTTCTCGCCAAAAGTGTTAATTGCCCGAATCGAATCCTTACTTAGACGAACCTA
TCAAAATAACGATATTATCAATATAGATGATCTGATCTTGGATCAAAATGCCAAACGCGTTACCTTCCAAAAAAAAGAAA
TTAGCTTAAGCAGTACCGAATATAAATTATTACACTTTTTTATGGCTCATCCGGAAAAAGTCTATAGCCGAGAACAATTA
CTGGATTTTGTTTGGGGCAATGATATTTATGTGGAAGACAGAACGGTCGATTCGTATATTCGTCGGTTAAGAAAAAGTTT
GGAACCCTGCGGTTTCGAGCGCTATGTGCAAACGGTACGCGGATCCGGTTATCGTTTTTCCAACCACTTTCAGGATAATT
AA

Upstream 100 bases:

>100_bases
AGAGCAGAAAGTTAGAAGCAGACGATGGGGCGCAAAGGATTAGGAGCATAAGATAAATGAAAAAATTCTGATTGTCGAAG
ATGAAAGAGCTATCCGAGAA

Downstream 100 bases:

>100_bases
TGAAAAAATTCAGCCTTTCTTTCAAGCATTTTTTTATCGAAGCGATGCTTGCCGTTGCGACGGCATTTTTATTCGGCTTA
TTTGCTTGGGACTTCCTGAC

Product: phosphate regulon transcriptional regulatory protein PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MISLFLLQQNYQVLEAEDYQSAVKKLSEKPKLILLDWMLPGRSGIQFIQYLKKSEETAQIPILMLTARSSEDDCIVCLNT
GADDYVTKPFSPKVLIARIESLLRRTYQNNDIINIDDLILDQNAKRVTFQKKEISLSSTEYKLLHFFMAHPEKVYSREQL
LDFVWGNDIYVEDRTVDSYIRRLRKSLEPCGFERYVQTVRGSGYRFSNHFQDN

Sequences:

>Translated_213_residues
MISLFLLQQNYQVLEAEDYQSAVKKLSEKPKLILLDWMLPGRSGIQFIQYLKKSEETAQIPILMLTARSSEDDCIVCLNT
GADDYVTKPFSPKVLIARIESLLRRTYQNNDIINIDDLILDQNAKRVTFQKKEISLSSTEYKLLHFFMAHPEKVYSREQL
LDFVWGNDIYVEDRTVDSYIRRLRKSLEPCGFERYVQTVRGSGYRFSNHFQDN
>Mature_213_residues
MISLFLLQQNYQVLEAEDYQSAVKKLSEKPKLILLDWMLPGRSGIQFIQYLKKSEETAQIPILMLTARSSEDDCIVCLNT
GADDYVTKPFSPKVLIARIESLLRRTYQNNDIINIDDLILDQNAKRVTFQKKEISLSSTEYKLLHFFMAHPEKVYSREQL
LDFVWGNDIYVEDRTVDSYIRRLRKSLEPCGFERYVQTVRGSGYRFSNHFQDN

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=213, Percent_Identity=57.7464788732394, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI1788394, Length=181, Percent_Identity=38.121546961326, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1789809, Length=216, Percent_Identity=32.4074074074074, Blast_Score=120, Evalue=5e-29,
Organism=Escherichia coli, GI2367329, Length=210, Percent_Identity=35.7142857142857, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI87082012, Length=181, Percent_Identity=35.3591160220994, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1786911, Length=202, Percent_Identity=34.6534653465347, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1790860, Length=207, Percent_Identity=34.7826086956522, Blast_Score=113, Evalue=8e-27,
Organism=Escherichia coli, GI1790863, Length=216, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=5e-26,
Organism=Escherichia coli, GI1786784, Length=175, Percent_Identity=34.2857142857143, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787375, Length=199, Percent_Identity=31.6582914572864, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI145693140, Length=224, Percent_Identity=32.5892857142857, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1790552, Length=174, Percent_Identity=34.4827586206897, Blast_Score=90, Evalue=9e-20,
Organism=Escherichia coli, GI1787229, Length=184, Percent_Identity=34.2391304347826, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1789402, Length=176, Percent_Identity=29.5454545454545, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 24978; Mature: 24978

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLFLLQQNYQVLEAEDYQSAVKKLSEKPKLILLDWMLPGRSGIQFIQYLKKSEETAQI
CEEEEEEECCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCC
PILMLTARSSEDDCIVCLNTGADDYVTKPFSPKVLIARIESLLRRTYQNNDIINIDDLIL
EEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHEE
DQNAKRVTFQKKEISLSSTEYKLLHFFMAHPEKVYSREQLLDFVWGNDIYVEDRTVDSYI
CCCCCEEEEEHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCHHHHH
RRLRKSLEPCGFERYVQTVRGSGYRFSNHFQDN
HHHHHHCCCCCHHHHHHHHCCCCCEECCCCCCC
>Mature Secondary Structure
MISLFLLQQNYQVLEAEDYQSAVKKLSEKPKLILLDWMLPGRSGIQFIQYLKKSEETAQI
CEEEEEEECCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCC
PILMLTARSSEDDCIVCLNTGADDYVTKPFSPKVLIARIESLLRRTYQNNDIINIDDLIL
EEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHEE
DQNAKRVTFQKKEISLSSTEYKLLHFFMAHPEKVYSREQLLDFVWGNDIYVEDRTVDSYI
CCCCCEEEEEHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCHHHHH
RRLRKSLEPCGFERYVQTVRGSGYRFSNHFQDN
HHHHHHCCCCCHHHHHHHHCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]