Click here to switch to the map view.

The map label for this gene is parE [H]

Identifier: 126208721

GI number: 126208721

Start: 1443581

End: 1445494

Strand: Reverse

Name: parE [H]

Synonym: APL_1255

Alternate gene names: 126208721

Gene position: 1445494-1443581 (Counterclockwise)

Preceding gene: 126208722

Following gene: 126208720

Centisome position: 63.55

GC content: 45.56

Gene sequence:

>1914_bases
ATGTCTGATAAACGCTACGGTGCCGATGAAATTACCGTTTTAAAAGATCTTGAACCCGTCCAACTTCGCCCCGGAATGTA
CACCGATACCACTCGTCCGAACCATTTAGGGCAAGAAGTTATTGATAATAGTGTCGATGAAGCGTTATCGGGCTATGCCT
CTCAAATCGATGTGATCCTGCATACGGATAATTCGTTGGAAGTCATTGATAACGGCCGAGGAATGCCGGTTGATATCCAC
TCTACCGAAAAAATTTCCGGTGTGGAATTGATTATGACCAAACTGCATGCAGGCGGTAAGTTTTCCAATAAAAACTACAC
GTTTTCAGGTGGTTTACACGGGGTAGGGATCTCGGTGGTAAACGCTCTTTCGCAACGTGTGGAAATCAAAATCAAGCGTA
ACGGCGAAATTTATACGATTGCCTTTGAAAACGGGGTAAAAGTTGAAGAATTAACCGTGATTGGCAGCTGTCCGAAAAAA
CAAACCGGTACGACCGTTCGTTTCTATCCTAACCCGAAATACTTTGATTCGCCGAGATTCTCGGTCAGCCGTTTACGCCA
CTTATTACGTGCCAAAGCGGTACTTTGTCCGAAATTAACCCTCAACTTCGTTGATCATATCAATAACAGCAGCGAAACTT
GGTATTACGAAGACGGTTTATCAGACTATTTAAGCGAAGCGTTAAAAGAATTCGAATGTTTACCGAATCCGCCGTTTATC
GGCGATGTCACATCCGAAACTGAAGCGGTAAGCTGGGCGTTAACTTGGTTGCCGGAAGGCGGTGAGTTAGTTGCCGAGAG
TTATGTGAACTTAATCCCGACCGCACAAGGCGGTACGCACGTAAACGGTTTACGTAACGGCTTATTAAAAGCAATGGTGG
AATTCTGCGAAATTCATAATTTACTGCCGAAAAGCGTGAAATTAACCGCCGATGACGTGTGGAATCGCTGCGCTTATGTG
CTTTCGTTGAAAATTCAAGAACCGCAATTTGCAGGGCAAACCAAAGAGCGTCTTTCCTCTCGTCAGGCTTCAAGCTATGT
GGATAGCACAATTAAAGATGCGTTCAGTTTATGGTTAAACCAAAACGTGCAAACCGGTAAATTGATTGCCGAAATGGCGA
TCTCATCGGCGCAAAGTCGTTTACGTGCGGCGAAAAAAGTAGTGCGTAAAAAGTTAGTGAGCGGCCCCGCATTACCGGGC
AAATTGGCCGATTGTACCTCGCAAGATTTAAGCCGTACCGAGCTGTTCTTAGTGGAAGGGGATTCTGCCGGCGGTTCGGC
GAAACAGGCGCGTGATAAGGATTATCAAGCGATTTTACCGCTACGAGGCAAAATTCTGAATACGTGGGAAGTGTCTTCCG
ATCAAGTGCTTGCTTCGCAAGAAGTTCACGATATTGCGGTAGCACTTGGGATTGATCCGGATAGCGATAGTTTAGATGAA
TTACGTTACGGTAAGGTATGTATTCTCGCCGATGCGGATTCGGACGGTTTACATATTGCAACCTTATTATGTGCCTTGTT
CTTGCGCCATTTCCCGAATTTAGTCAAAAACGGTCACGTTTATGTGGCGATGCCGCCGCTTTATCGGATTGATATCGGTA
AAGACGAAGTACATTACGCCCTTGACGAAGCGGAAAAAGAAGCGATTTTAGCTCGTCTTGCCAAGAAAAAAGGTAAACCG
AACGTACAGCGTTTTAAAGGTTTGGGGGAGATGAACCCGAGCCAATTACGTGAAACTACCATGGATCCGAGTACGCGCCG
TTTGGTTCAGCTTACCCTTGATGAATTGGACGTAGCCGAAGAAAACGAGCCGGATACCTTTGAAATTATGGATATGTTAC
TGGCGAAGAAACGTGCGGAAGACCGTAAACAATGGCTACAAAATCGAGGCGATGAAGCGGAATTAAGCGTTTAA

Upstream 100 bases:

>100_bases
TTGGTGAAACAAAAAATCATTAAAACAGTATTTTTTACTCAACAAATGGGGAATTTGCCAGTAAAATGCCTGCTTTTCTG
TCCACAAAGAGAGCTTAGTC

Downstream 100 bases:

>100_bases
TTTGATAGAATATTCAAGCGGTCGATTTTTTTAGTTTTTTTGTAAAAAGCGAGTAAAAATCGACCGCTTGTGTTTATGTG
AATTTCGTTAAAAATTTTTT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 637; Mature: 636

Protein sequence:

>637_residues
MSDKRYGADEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNSVDEALSGYASQIDVILHTDNSLEVIDNGRGMPVDIH
STEKISGVELIMTKLHAGGKFSNKNYTFSGGLHGVGISVVNALSQRVEIKIKRNGEIYTIAFENGVKVEELTVIGSCPKK
QTGTTVRFYPNPKYFDSPRFSVSRLRHLLRAKAVLCPKLTLNFVDHINNSSETWYYEDGLSDYLSEALKEFECLPNPPFI
GDVTSETEAVSWALTWLPEGGELVAESYVNLIPTAQGGTHVNGLRNGLLKAMVEFCEIHNLLPKSVKLTADDVWNRCAYV
LSLKIQEPQFAGQTKERLSSRQASSYVDSTIKDAFSLWLNQNVQTGKLIAEMAISSAQSRLRAAKKVVRKKLVSGPALPG
KLADCTSQDLSRTELFLVEGDSAGGSAKQARDKDYQAILPLRGKILNTWEVSSDQVLASQEVHDIAVALGIDPDSDSLDE
LRYGKVCILADADSDGLHIATLLCALFLRHFPNLVKNGHVYVAMPPLYRIDIGKDEVHYALDEAEKEAILARLAKKKGKP
NVQRFKGLGEMNPSQLRETTMDPSTRRLVQLTLDELDVAEENEPDTFEIMDMLLAKKRAEDRKQWLQNRGDEAELSV

Sequences:

>Translated_637_residues
MSDKRYGADEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNSVDEALSGYASQIDVILHTDNSLEVIDNGRGMPVDIH
STEKISGVELIMTKLHAGGKFSNKNYTFSGGLHGVGISVVNALSQRVEIKIKRNGEIYTIAFENGVKVEELTVIGSCPKK
QTGTTVRFYPNPKYFDSPRFSVSRLRHLLRAKAVLCPKLTLNFVDHINNSSETWYYEDGLSDYLSEALKEFECLPNPPFI
GDVTSETEAVSWALTWLPEGGELVAESYVNLIPTAQGGTHVNGLRNGLLKAMVEFCEIHNLLPKSVKLTADDVWNRCAYV
LSLKIQEPQFAGQTKERLSSRQASSYVDSTIKDAFSLWLNQNVQTGKLIAEMAISSAQSRLRAAKKVVRKKLVSGPALPG
KLADCTSQDLSRTELFLVEGDSAGGSAKQARDKDYQAILPLRGKILNTWEVSSDQVLASQEVHDIAVALGIDPDSDSLDE
LRYGKVCILADADSDGLHIATLLCALFLRHFPNLVKNGHVYVAMPPLYRIDIGKDEVHYALDEAEKEAILARLAKKKGKP
NVQRFKGLGEMNPSQLRETTMDPSTRRLVQLTLDELDVAEENEPDTFEIMDMLLAKKRAEDRKQWLQNRGDEAELSV
>Mature_636_residues
SDKRYGADEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNSVDEALSGYASQIDVILHTDNSLEVIDNGRGMPVDIHS
TEKISGVELIMTKLHAGGKFSNKNYTFSGGLHGVGISVVNALSQRVEIKIKRNGEIYTIAFENGVKVEELTVIGSCPKKQ
TGTTVRFYPNPKYFDSPRFSVSRLRHLLRAKAVLCPKLTLNFVDHINNSSETWYYEDGLSDYLSEALKEFECLPNPPFIG
DVTSETEAVSWALTWLPEGGELVAESYVNLIPTAQGGTHVNGLRNGLLKAMVEFCEIHNLLPKSVKLTADDVWNRCAYVL
SLKIQEPQFAGQTKERLSSRQASSYVDSTIKDAFSLWLNQNVQTGKLIAEMAISSAQSRLRAAKKVVRKKLVSGPALPGK
LADCTSQDLSRTELFLVEGDSAGGSAKQARDKDYQAILPLRGKILNTWEVSSDQVLASQEVHDIAVALGIDPDSDSLDEL
RYGKVCILADADSDGLHIATLLCALFLRHFPNLVKNGHVYVAMPPLYRIDIGKDEVHYALDEAEKEAILARLAKKKGKPN
VQRFKGLGEMNPSQLRETTMDPSTRRLVQLTLDELDVAEENEPDTFEIMDMLLAKKRAEDRKQWLQNRGDEAELSV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=591, Percent_Identity=25.8883248730964, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI19913406, Length=376, Percent_Identity=28.4574468085106, Blast_Score=122, Evalue=7e-28,
Organism=Escherichia coli, GI1789408, Length=632, Percent_Identity=72.7848101265823, Blast_Score=986, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=552, Percent_Identity=40.5797101449275, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17535065, Length=588, Percent_Identity=25.6802721088435, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=594, Percent_Identity=23.5690235690236, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=235, Percent_Identity=28.5106382978723, Blast_Score=79, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=647, Percent_Identity=24.7295208655332, Blast_Score=137, Evalue=7e-33,
Organism=Drosophila melanogaster, GI17136538, Length=596, Percent_Identity=23.993288590604, Blast_Score=132, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 70690; Mature: 70559

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKRYGADEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNSVDEALSGYASQIDVIL
CCCCCCCCCCEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEE
HTDNSLEVIDNGRGMPVDIHSTEKISGVELIMTKLHAGGKFSNKNYTFSGGLHGVGISVV
ECCCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHH
NALSQRVEIKIKRNGEIYTIAFENGVKVEELTVIGSCPKKQTGTTVRFYPNPKYFDSPRF
HHHHHCEEEEEECCCCEEEEEECCCCEEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCH
SVSRLRHLLRAKAVLCPKLTLNFVDHINNSSETWYYEDGLSDYLSEALKEFECLPNPPFI
HHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCC
GDVTSETEAVSWALTWLPEGGELVAESYVNLIPTAQGGTHVNGLRNGLLKAMVEFCEIHN
CCCCCCCHHEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LLPKSVKLTADDVWNRCAYVLSLKIQEPQFAGQTKERLSSRQASSYVDSTIKDAFSLWLN
CCCCCEEECHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QNVQTGKLIAEMAISSAQSRLRAAKKVVRKKLVSGPALPGKLADCTSQDLSRTELFLVEG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEEEEEC
DSAGGSAKQARDKDYQAILPLRGKILNTWEVSSDQVLASQEVHDIAVALGIDPDSDSLDE
CCCCCCCHHHCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHEEEEEEEECCCCCCCHHH
LRYGKVCILADADSDGLHIATLLCALFLRHFPNLVKNGHVYVAMPPLYRIDIGKDEVHYA
HCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCCHHHHH
LDEAEKEAILARLAKKKGKPNVQRFKGLGEMNPSQLRETTMDPSTRRLVQLTLDELDVAE
HHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCC
ENEPDTFEIMDMLLAKKRAEDRKQWLQNRGDEAELSV
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SDKRYGADEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNSVDEALSGYASQIDVIL
CCCCCCCCCEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEE
HTDNSLEVIDNGRGMPVDIHSTEKISGVELIMTKLHAGGKFSNKNYTFSGGLHGVGISVV
ECCCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHH
NALSQRVEIKIKRNGEIYTIAFENGVKVEELTVIGSCPKKQTGTTVRFYPNPKYFDSPRF
HHHHHCEEEEEECCCCEEEEEECCCCEEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCH
SVSRLRHLLRAKAVLCPKLTLNFVDHINNSSETWYYEDGLSDYLSEALKEFECLPNPPFI
HHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCC
GDVTSETEAVSWALTWLPEGGELVAESYVNLIPTAQGGTHVNGLRNGLLKAMVEFCEIHN
CCCCCCCHHEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LLPKSVKLTADDVWNRCAYVLSLKIQEPQFAGQTKERLSSRQASSYVDSTIKDAFSLWLN
CCCCCEEECHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QNVQTGKLIAEMAISSAQSRLRAAKKVVRKKLVSGPALPGKLADCTSQDLSRTELFLVEG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEEEEEC
DSAGGSAKQARDKDYQAILPLRGKILNTWEVSSDQVLASQEVHDIAVALGIDPDSDSLDE
CCCCCCCHHHCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHEEEEEEEECCCCCCCHHH
LRYGKVCILADADSDGLHIATLLCALFLRHFPNLVKNGHVYVAMPPLYRIDIGKDEVHYA
HCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCCHHHHH
LDEAEKEAILARLAKKKGKPNVQRFKGLGEMNPSQLRETTMDPSTRRLVQLTLDELDVAE
HHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCC
ENEPDTFEIMDMLLAKKRAEDRKQWLQNRGDEAELSV
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]