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The map label for this gene is ydgI [H]

Identifier: 126208712

GI number: 126208712

Start: 1433543

End: 1434181

Strand: Reverse

Name: ydgI [H]

Synonym: APL_1246

Alternate gene names: 126208712

Gene position: 1434181-1433543 (Counterclockwise)

Preceding gene: 126208713

Following gene: 126208711

Centisome position: 63.06

GC content: 44.91

Gene sequence:

>639_bases
ATGGAACTGAAAAATGTGATTCAGCAACGAAAAACCGTCAAAGTTTTTAATCCGAATGTAAAAATAGAACGCCAAGAATT
AGAGGAAATGTTAGGCTTGGCGCAGCTTGCGCCGTCTAAAGCAAATTTGCAACCGTGGCGTTTTGTCGTGGTGGACGATG
TAGAACAGAAAAGTAAATTACTCGGCTCGGTTGCCTTCAACGTACCGCCTTGTGAAACGGCTTCTGCGGTGATTCTCGTA
TTAGCGGATTTACAATATCATCTGCTACTGGATGATATTTTGGATCGCTCGATTGAGACCGGTTGCTTACATCAACAATT
TAAACAACGTTCATTGGATTTTCTCCTTAGCGTACATAATGCGGCAACCGAACAGGATATTCGCGACCAAGTGGTTACCG
ATACCAGTTTAGCCGCCATGCAATTAATGTTAATTGCCAAAGATAAAGGTTATGATACCCATGCTATCGGTGTATTTGAC
CGTCCGGCGGTATTAGCCGTACTTGAGGTGGATGCCGAGCGTTATTTACCGGTGATGCTATTAGCGATTGGTAAGGCGGC
AACGGCGGCACTGCCGAGCAGTCGTTTACCTCTCGAATATACCGTTGCTTGGAATAGCGGGCAGGGCTTTAAAAAGTAA

Upstream 100 bases:

>100_bases
CTAGTGCATAAAAAGTGTTAAATACCTCATTTAGACTATTTCTCTTTAGATTGGCTAAACGTACACTGGTATAAATAATA
ATCTATAGAAGGAATGAAAA

Downstream 100 bases:

>100_bases
TTGTAATATTTGTTAAACAAGCGGTCTGATTTCTTAAAACTTTTGCAAAAAGTTGCTAAAATTAGACCGCTTTTTTAGTG
CTATTTTATATTTGAGGAAG

Product: hypothetical protein

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MELKNVIQQRKTVKVFNPNVKIERQELEEMLGLAQLAPSKANLQPWRFVVVDDVEQKSKLLGSVAFNVPPCETASAVILV
LADLQYHLLLDDILDRSIETGCLHQQFKQRSLDFLLSVHNAATEQDIRDQVVTDTSLAAMQLMLIAKDKGYDTHAIGVFD
RPAVLAVLEVDAERYLPVMLLAIGKAATAALPSSRLPLEYTVAWNSGQGFKK

Sequences:

>Translated_212_residues
MELKNVIQQRKTVKVFNPNVKIERQELEEMLGLAQLAPSKANLQPWRFVVVDDVEQKSKLLGSVAFNVPPCETASAVILV
LADLQYHLLLDDILDRSIETGCLHQQFKQRSLDFLLSVHNAATEQDIRDQVVTDTSLAAMQLMLIAKDKGYDTHAIGVFD
RPAVLAVLEVDAERYLPVMLLAIGKAATAALPSSRLPLEYTVAWNSGQGFKK
>Mature_212_residues
MELKNVIQQRKTVKVFNPNVKIERQELEEMLGLAQLAPSKANLQPWRFVVVDDVEQKSKLLGSVAFNVPPCETASAVILV
LADLQYHLLLDDILDRSIETGCLHQQFKQRSLDFLLSVHNAATEQDIRDQVVTDTSLAAMQLMLIAKDKGYDTHAIGVFD
RPAVLAVLEVDAERYLPVMLLAIGKAATAALPSSRLPLEYTVAWNSGQGFKK

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23567; Mature: 23567

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKNVIQQRKTVKVFNPNVKIERQELEEMLGLAQLAPSKANLQPWRFVVVDDVEQKSKL
CCHHHHHHHHCEEEEECCCCEEEHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHH
LGSVAFNVPPCETASAVILVLADLQYHLLLDDILDRSIETGCLHQQFKQRSLDFLLSVHN
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AATEQDIRDQVVTDTSLAAMQLMLIAKDKGYDTHAIGVFDRPAVLAVLEVDAERYLPVML
HHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCCEEEECCCCCCEEEEEECCHHHHHHHHH
LAIGKAATAALPSSRLPLEYTVAWNSGQGFKK
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MELKNVIQQRKTVKVFNPNVKIERQELEEMLGLAQLAPSKANLQPWRFVVVDDVEQKSKL
CCHHHHHHHHCEEEEECCCCEEEHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHH
LGSVAFNVPPCETASAVILVLADLQYHLLLDDILDRSIETGCLHQQFKQRSLDFLLSVHN
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AATEQDIRDQVVTDTSLAAMQLMLIAKDKGYDTHAIGVFDRPAVLAVLEVDAERYLPVML
HHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCCEEEECCCCCCEEEEEECCHHHHHHHHH
LAIGKAATAALPSSRLPLEYTVAWNSGQGFKK
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]