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The map label for this gene is hgiDIIM [H]

Identifier: 126208670

GI number: 126208670

Start: 1384691

End: 1385785

Strand: Reverse

Name: hgiDIIM [H]

Synonym: APL_1202

Alternate gene names: 126208670

Gene position: 1385785-1384691 (Counterclockwise)

Preceding gene: 126208672

Following gene: 126208669

Centisome position: 60.93

GC content: 38.54

Gene sequence:

>1095_bases
ATGTCATACAATTGGCATATAGAGTTTAATATGGTTGAAAATATGATGAAACAATTGAAAGCCGTTGATTTTTTCTGTGG
CGGCGGGGGAATGAGTTACGGTCTACAAAAAGCGGGTATTCGAATACTTGCAGGGATCGATTACGAAATAAATTGTAAAG
AAACTTACGAGACGAATATAAAAGGAGCTTCCTTTATTCATGCAAACGTTTTTGAATTAACGGAAAAAGAATTAGAAAAG
ACTCTAGATATTTCTAGAAAAGACGATAATTTGATTTTAGTCGGTTGTAGCCCCTGCCAATATTGGAGTGTTATTCGAAC
CAGTAAAGAAAAATCGGAAAAATCTAAAAGCCTACTGTCCGAATTTCAACGATTCGTTGAATATTTTGTTCCGGGATATG
TTGTTGTAGAAAATGTCCCGGGGATTTTCACTCGTCAAGAAGAAAGCGGTCTGGATATTTTTGTACGAAGATTGGAAGAA
TTGGGGTATACGGTTCATTTCGGAATACACAACACAAAGAACTACGGTGTTCCGCAAAGTCGTAAACGTTTCACTTTAAT
TGCAAATCGAGTAACTCAAGATAAATTAGAGCCTTTAGAATTAAAAGACAAAGTACTAACGGTTCGAGACGTATTGGGCG
AAAAAAACGGATTTCCTAAAATACCAGCAGGTCACCAAGATGTAAGCGAATATCTGCATAGTTGCGCAGGATTATCCGAA
ATTAATATACGGCGTTTGCGTTTAGTGCCTAAAGACGGCGGCACTCGCCTAGCTTTCGCTGATAGACCTGACTTACAACT
TAAATGCTTTATTGGAAAAGATAACTACTTTAAAGATACATTCGGTCGTTTATGGTGGGATCAGCCTTCGCCTACGATTA
CAACAAAATTTTTCAGTATTTCCAACGGGCGTTTTGCTCATCCGGAAGAAGATCGGGCCTTGTCTTTACGTGAAGGAGCT
ACTTTACAATCCTTTCCTAAAGATTATGTGTTTAAGGCGAAGGGAAGGGAGGCTATAGCGCGATTAATCGGTAATGCCGT
TCCGCCTAAATATGCCGAACAAATCGGTAAAGCGATAATTAGTAACTTGAAATAA

Upstream 100 bases:

>100_bases
TAATTTTTAACAATCAATGAGAAAAGAATCTTACTTGTTGTCTAGAATAGCTTCCTTATTAAAGGATATACGGTTATCAT
TTCCTGAAAAAGAAATAGCA

Downstream 100 bases:

>100_bases
TATATGGAGATTAAAATGTCAGGAAAACAATTAAAAATGACTTTTCAGCCGACTGTGATCGAACATCTCGGAGTAAAAAT
GTATTCACATACCGTTCCTG

Product: modification methylase

Products: NA

Alternate protein names: M.HgiDII; Cytosine-specific methyltransferase HgiDII [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSYNWHIEFNMVENMMKQLKAVDFFCGGGGMSYGLQKAGIRILAGIDYEINCKETYETNIKGASFIHANVFELTEKELEK
TLDISRKDDNLILVGCSPCQYWSVIRTSKEKSEKSKSLLSEFQRFVEYFVPGYVVVENVPGIFTRQEESGLDIFVRRLEE
LGYTVHFGIHNTKNYGVPQSRKRFTLIANRVTQDKLEPLELKDKVLTVRDVLGEKNGFPKIPAGHQDVSEYLHSCAGLSE
INIRRLRLVPKDGGTRLAFADRPDLQLKCFIGKDNYFKDTFGRLWWDQPSPTITTKFFSISNGRFAHPEEDRALSLREGA
TLQSFPKDYVFKAKGREAIARLIGNAVPPKYAEQIGKAIISNLK

Sequences:

>Translated_364_residues
MSYNWHIEFNMVENMMKQLKAVDFFCGGGGMSYGLQKAGIRILAGIDYEINCKETYETNIKGASFIHANVFELTEKELEK
TLDISRKDDNLILVGCSPCQYWSVIRTSKEKSEKSKSLLSEFQRFVEYFVPGYVVVENVPGIFTRQEESGLDIFVRRLEE
LGYTVHFGIHNTKNYGVPQSRKRFTLIANRVTQDKLEPLELKDKVLTVRDVLGEKNGFPKIPAGHQDVSEYLHSCAGLSE
INIRRLRLVPKDGGTRLAFADRPDLQLKCFIGKDNYFKDTFGRLWWDQPSPTITTKFFSISNGRFAHPEEDRALSLREGA
TLQSFPKDYVFKAKGREAIARLIGNAVPPKYAEQIGKAIISNLK
>Mature_363_residues
SYNWHIEFNMVENMMKQLKAVDFFCGGGGMSYGLQKAGIRILAGIDYEINCKETYETNIKGASFIHANVFELTEKELEKT
LDISRKDDNLILVGCSPCQYWSVIRTSKEKSEKSKSLLSEFQRFVEYFVPGYVVVENVPGIFTRQEESGLDIFVRRLEEL
GYTVHFGIHNTKNYGVPQSRKRFTLIANRVTQDKLEPLELKDKVLTVRDVLGEKNGFPKIPAGHQDVSEYLHSCAGLSEI
NIRRLRLVPKDGGTRLAFADRPDLQLKCFIGKDNYFKDTFGRLWWDQPSPTITTKFFSISNGRFAHPEEDRALSLREGAT
LQSFPKDYVFKAKGREAIARLIGNAVPPKYAEQIGKAIISNLK

Specific function: This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the HgiDII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4503351, Length=186, Percent_Identity=26.8817204301075, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI195927037, Length=186, Percent_Identity=26.8817204301075, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 41473; Mature: 41342

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYNWHIEFNMVENMMKQLKAVDFFCGGGGMSYGLQKAGIRILAGIDYEINCKETYETNI
CCEEEEEEHHHHHHHHHHHHHHEEEECCCCCCCCHHHCCEEEEEECCEEECCCHHHCCCC
KGASFIHANVFELTEKELEKTLDISRKDDNLILVGCSPCQYWSVIRTSKEKSEKSKSLLS
CCCEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EFQRFVEYFVPGYVVVENVPGIFTRQEESGLDIFVRRLEELGYTVHFGIHNTKNYGVPQS
HHHHHHHHHCCCEEEEECCCCCEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCC
RKRFTLIANRVTQDKLEPLELKDKVLTVRDVLGEKNGFPKIPAGHQDVSEYLHSCAGLSE
CCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCC
INIRRLRLVPKDGGTRLAFADRPDLQLKCFIGKDNYFKDTFGRLWWDQPSPTITTKFFSI
CCEEEEEEEECCCCCEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCEEEEEEEEE
SNGRFAHPEEDRALSLREGATLQSFPKDYVFKAKGREAIARLIGNAVPPKYAEQIGKAII
CCCCCCCCCCCCEEECCCCCCHHHCCHHHEEECCCHHHHHHHHCCCCCHHHHHHHHHHHH
SNLK
HCCC
>Mature Secondary Structure 
SYNWHIEFNMVENMMKQLKAVDFFCGGGGMSYGLQKAGIRILAGIDYEINCKETYETNI
CEEEEEEHHHHHHHHHHHHHHEEEECCCCCCCCHHHCCEEEEEECCEEECCCHHHCCCC
KGASFIHANVFELTEKELEKTLDISRKDDNLILVGCSPCQYWSVIRTSKEKSEKSKSLLS
CCCEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EFQRFVEYFVPGYVVVENVPGIFTRQEESGLDIFVRRLEELGYTVHFGIHNTKNYGVPQS
HHHHHHHHHCCCEEEEECCCCCEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCC
RKRFTLIANRVTQDKLEPLELKDKVLTVRDVLGEKNGFPKIPAGHQDVSEYLHSCAGLSE
CCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCC
INIRRLRLVPKDGGTRLAFADRPDLQLKCFIGKDNYFKDTFGRLWWDQPSPTITTKFFSI
CCEEEEEEEECCCCCEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCEEEEEEEEE
SNGRFAHPEEDRALSLREGATLQSFPKDYVFKAKGREAIARLIGNAVPPKYAEQIGKAII
CCCCCCCCCCCCEEECCCCCCHHHCCHHHEEECCCHHHHHHHHCCCCCHHHHHHHHHHHH
SNLK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1937045; 7607523 [H]