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The map label for this gene is rluB [H]

Identifier: 126208628

GI number: 126208628

Start: 1336910

End: 1337959

Strand: Reverse

Name: rluB [H]

Synonym: APL_1160

Alternate gene names: 126208628

Gene position: 1337959-1336910 (Counterclockwise)

Preceding gene: 126208629

Following gene: 126208627

Centisome position: 58.82

GC content: 43.62

Gene sequence:

>1050_bases
ATGAAAACATTTCAAACAAAAAATGCCGATTCGAAATCGGCAAAATTTTCTAAAAATCCACGTTTAGCCGAGCGTAATGA
AAAGCGTGCCGAACGCCCGACCTCAAAACCGTTAAAAGCAAAAAGTGAGCAATATTTTGCAAAAAAATCAGCAAAACCGA
CCGCTTCCATCACTGAAACCTCAGAGAAGAAAGAGAAAGTAGTCGGTGAAAAACTACAGAAAATTCTAGCGCGTGCCGGT
CAGGGATCTCGCCGTGAGTTGGAAGAGATTATTGCGGCAGGGCGTGTAAGTGTTGATGGGAAAATCGCAAGCTTAGGCGA
TCGTGTGCAAGTAAGCAGTGCAACTAAAATTCGTATTGACGGTAATTTAATCAGCCTAATTCCGGTACAAAAAGAAATTT
GTCGCGTATTGATGTATTACAAACCGGAAGGCGAATTGTGTACTCGTTCAGATCCGGAAGGGCGTGCAACGGTATTTGAT
CGCTTACCTCGTTTAACCGGAGCGCGTTGGATTGCGGTAGGTCGTTTAGATATTAATACTTCGGGTTTATTACTGTTTAC
TACGGACGGTGAGTTAGCGAATCGTTTAATGCACCCGAGTCGTGAAGTAGAGCGTGAATATTCCGTGCGTGTGTTCGGTA
ATATTGATGAAGCAATGTTACAACGTTTACGTAAGGGTGTTCAGTTAGAAGACGGTCCGGCAAACTTTAAACAGATTAAA
ACGGTCGGCGGCACAGGTTTAAACCAATGGTTTGATGTCACTTTAACCGAAGGACGTAACCGTGAAGTTCGTCGTTTATG
GGAATCGCAAGGTGTTGAGGTTAGTCGTTTGATTCGTATTCGTTACGGTAATATCAAATTGGAAAAAGGTTTACCGCGCG
GCGGTTGGGAAGAAATGGGCTTAGAGCAAGTAAACTATTTGCGTGAGCTTGTCGGCCTACCGGCGGAAACCGAAACCAAA
GTCGATGTGACGAAAAATCGCCGTCGTACCAATATTCGTCAAATTCGTAAAGCGGTAAAACAGCATTCTAAATACAGAAC
GAAAGCGTAA

Upstream 100 bases:

>100_bases
GATCGGGCGATATTACGCCGTTTAATTAATGATTTTCTCCTTCTTAAAGGAGGATATATATATTTTTAAAGACGCTCGTG
AGAGCGACAAAGGAATCTTA

Downstream 100 bases:

>100_bases
AACAAACCTAGATAAGCCAATATGAGAAATATTGGCTTTTTTATCTTTACATAATAATTGTAAATTTTGTTTATTTATTT
AAAGTTGTTTGTGTATAATT

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKTFQTKNADSKSAKFSKNPRLAERNEKRAERPTSKPLKAKSEQYFAKKSAKPTASITETSEKKEKVVGEKLQKILARAG
QGSRRELEEIIAAGRVSVDGKIASLGDRVQVSSATKIRIDGNLISLIPVQKEICRVLMYYKPEGELCTRSDPEGRATVFD
RLPRLTGARWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVRVFGNIDEAMLQRLRKGVQLEDGPANFKQIK
TVGGTGLNQWFDVTLTEGRNREVRRLWESQGVEVSRLIRIRYGNIKLEKGLPRGGWEEMGLEQVNYLRELVGLPAETETK
VDVTKNRRRTNIRQIRKAVKQHSKYRTKA

Sequences:

>Translated_349_residues
MKTFQTKNADSKSAKFSKNPRLAERNEKRAERPTSKPLKAKSEQYFAKKSAKPTASITETSEKKEKVVGEKLQKILARAG
QGSRRELEEIIAAGRVSVDGKIASLGDRVQVSSATKIRIDGNLISLIPVQKEICRVLMYYKPEGELCTRSDPEGRATVFD
RLPRLTGARWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVRVFGNIDEAMLQRLRKGVQLEDGPANFKQIK
TVGGTGLNQWFDVTLTEGRNREVRRLWESQGVEVSRLIRIRYGNIKLEKGLPRGGWEEMGLEQVNYLRELVGLPAETETK
VDVTKNRRRTNIRQIRKAVKQHSKYRTKA
>Mature_349_residues
MKTFQTKNADSKSAKFSKNPRLAERNEKRAERPTSKPLKAKSEQYFAKKSAKPTASITETSEKKEKVVGEKLQKILARAG
QGSRRELEEIIAAGRVSVDGKIASLGDRVQVSSATKIRIDGNLISLIPVQKEICRVLMYYKPEGELCTRSDPEGRATVFD
RLPRLTGARWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVRVFGNIDEAMLQRLRKGVQLEDGPANFKQIK
TVGGTGLNQWFDVTLTEGRNREVRRLWESQGVEVSRLIRIRYGNIKLEKGLPRGGWEEMGLEQVNYLRELVGLPAETETK
VDVTKNRRRTNIRQIRKAVKQHSKYRTKA

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=277, Percent_Identity=74.3682310469314, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI1790453, Length=241, Percent_Identity=30.7053941908714, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788510, Length=239, Percent_Identity=29.2887029288703, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87081838, Length=185, Percent_Identity=32.972972972973, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 39591; Mature: 39591

Theoretical pI: Translated: 10.82; Mature: 10.82

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFQTKNADSKSAKFSKNPRLAERNEKRAERPTSKPLKAKSEQYFAKKSAKPTASITET
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHH
SEKKEKVVGEKLQKILARAGQGSRRELEEIIAAGRVSVDGKIASLGDRVQVSSATKIRID
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHCCCCEEEECCCEEEEEC
GNLISLIPVQKEICRVLMYYKPEGELCTRSDPEGRATVFDRLPRLTGARWIAVGRLDINT
CCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEEECC
SGLLLFTTDGELANRLMHPSREVEREYSVRVFGNIDEAMLQRLRKGVQLEDGPANFKQIK
CCEEEEECCHHHHHHHCCCCHHHHHHCEEEEECCCHHHHHHHHHCCCEECCCCCCHHHHH
TVGGTGLNQWFDVTLTEGRNREVRRLWESQGVEVSRLIRIRYGNIKLEKGLPRGGWEEMG
HCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEHEEEEEECCEEEECCCCCCCHHHHC
LEQVNYLRELVGLPAETETKVDVTKNRRRTNIRQIRKAVKQHSKYRTKA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTFQTKNADSKSAKFSKNPRLAERNEKRAERPTSKPLKAKSEQYFAKKSAKPTASITET
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHH
SEKKEKVVGEKLQKILARAGQGSRRELEEIIAAGRVSVDGKIASLGDRVQVSSATKIRID
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHCCCCEEEECCCEEEEEC
GNLISLIPVQKEICRVLMYYKPEGELCTRSDPEGRATVFDRLPRLTGARWIAVGRLDINT
CCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEEECC
SGLLLFTTDGELANRLMHPSREVEREYSVRVFGNIDEAMLQRLRKGVQLEDGPANFKQIK
CCEEEEECCHHHHHHHCCCCHHHHHHCEEEEECCCHHHHHHHHHCCCEECCCCCCHHHHH
TVGGTGLNQWFDVTLTEGRNREVRRLWESQGVEVSRLIRIRYGNIKLEKGLPRGGWEEMG
HCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEHEEEEEECCEEEECCCCCCCHHHHC
LEQVNYLRELVGLPAETETKVDVTKNRRRTNIRQIRKAVKQHSKYRTKA
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]