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The map label for this gene is pfkA

Identifier: 126208594

GI number: 126208594

Start: 1299786

End: 1300760

Strand: Reverse

Name: pfkA

Synonym: APL_1124

Alternate gene names: 126208594

Gene position: 1300760-1299786 (Counterclockwise)

Preceding gene: 126208595

Following gene: 126208593

Centisome position: 57.19

GC content: 45.85

Gene sequence:

>975_bases
ATGGCTAAACAAATCAAAAAAATTGCCGTGCTAACCAGTGGAGGTGATGCACCGGGTATGAATGCCGCAATTCGTGGCGT
AGTCCGCGCCGCATTAAACGAAGGCTTAGAAGTATATGGCGTACAAGACGGTTATTACGGTTTATATACCGATAGAGTAA
TTCCGCTTGATCGTCGTTCCGTATCGGAAACGATCAACCGCGGCGGTACTTTTTTAGGTTCGGCGCGCTTCCCGCAGTTT
AAAGATCCTGACGTACGTAAAAAATCGGTAGAAACCTTAAAAAAATACGATATTGATGCGCTGGTCGTTATCGGCGGAGA
CGGCTCGTATATGGGAGCGAAACTTATAACCGAAGAATTCGGTTATCCGTGTATCGGTATTCCGGGTACGATCGATAATG
ATATCGTCGGTACGGATTATACTATCGGTTACCAAACCGCATTGGAAACGGCGGTGGAAGCGATTGACCGTTTACGCGAT
ACTTCTACCTCGCATCAGCGTATTTCAATCGTTGAAATTATGGGACGCCACTGCGGCGATTTAACCATTAGTGCGGCGCT
TGCCAGCGGTTGCGAATATATTATCGTGCCGGAAAAAGGTTTGGATAAAGAGTCGCTAATGCGTAATATCGAAGACGGTT
TTAATAAAGGTAAACGTCATGCGATTATTGCGATTACCGAATTAATGACGGACGTTCAGGCATTGGCAAAAGAAATCGAA
GACCGCTTCGGTCATGAAACGCGCGCAACCGTATTAGGTCACATTCAACGTGGCGGTGCACCTTGCCCGTTCGACCGTAT
TTTAGCTTCCCGTATGGGCGTATATGCGGTGGATCTGTTATTACAAGGTCACGGCGGTCGTTGTATCGGTATCAAAAATG
AGAATTTGGTACATCACGATATTATTGATGCGATTAACAATATGCGCCGCCCGTTCAAAGAAGAACTGTTTGAGGCGGCT
AGAAAATTATTCTAA

Upstream 100 bases:

>100_bases
AGATGAAATAGCACTAGAATGAATACCCTAAATAAGGTAATCTTGCCGCCAATTTTCGATTTATTTTAAAATTTAAATTT
ATAGCAAAAGGTGTCAAAAA

Downstream 100 bases:

>100_bases
TTTTCCTTAGAATACCGAGGCGACTATGTTAGTCGCCTTTTGTTTTTAGGTAGGATAGTTATGCTGAAAGATAAACCGGT
CATTTCAAATCAACACGGCG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MAKQIKKIAVLTSGGDAPGMNAAIRGVVRAALNEGLEVYGVQDGYYGLYTDRVIPLDRRSVSETINRGGTFLGSARFPQF
KDPDVRKKSVETLKKYDIDALVVIGGDGSYMGAKLITEEFGYPCIGIPGTIDNDIVGTDYTIGYQTALETAVEAIDRLRD
TSTSHQRISIVEIMGRHCGDLTISAALASGCEYIIVPEKGLDKESLMRNIEDGFNKGKRHAIIAITELMTDVQALAKEIE
DRFGHETRATVLGHIQRGGAPCPFDRILASRMGVYAVDLLLQGHGGRCIGIKNENLVHHDIIDAINNMRRPFKEELFEAA
RKLF

Sequences:

>Translated_324_residues
MAKQIKKIAVLTSGGDAPGMNAAIRGVVRAALNEGLEVYGVQDGYYGLYTDRVIPLDRRSVSETINRGGTFLGSARFPQF
KDPDVRKKSVETLKKYDIDALVVIGGDGSYMGAKLITEEFGYPCIGIPGTIDNDIVGTDYTIGYQTALETAVEAIDRLRD
TSTSHQRISIVEIMGRHCGDLTISAALASGCEYIIVPEKGLDKESLMRNIEDGFNKGKRHAIIAITELMTDVQALAKEIE
DRFGHETRATVLGHIQRGGAPCPFDRILASRMGVYAVDLLLQGHGGRCIGIKNENLVHHDIIDAINNMRRPFKEELFEAA
RKLF
>Mature_323_residues
AKQIKKIAVLTSGGDAPGMNAAIRGVVRAALNEGLEVYGVQDGYYGLYTDRVIPLDRRSVSETINRGGTFLGSARFPQFK
DPDVRKKSVETLKKYDIDALVVIGGDGSYMGAKLITEEFGYPCIGIPGTIDNDIVGTDYTIGYQTALETAVEAIDRLRDT
STSHQRISIVEIMGRHCGDLTISAALASGCEYIIVPEKGLDKESLMRNIEDGFNKGKRHAIIAITELMTDVQALAKEIED
RFGHETRATVLGHIQRGGAPCPFDRILASRMGVYAVDLLLQGHGGRCIGIKNENLVHHDIIDAINNMRRPFKEELFEAAR
KLF

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI266453768, Length=358, Percent_Identity=37.9888268156425, Blast_Score=211, Evalue=7e-55,
Organism=Homo sapiens, GI266453748, Length=358, Percent_Identity=37.9888268156425, Blast_Score=211, Evalue=7e-55,
Organism=Homo sapiens, GI4505749, Length=358, Percent_Identity=37.9888268156425, Blast_Score=211, Evalue=7e-55,
Organism=Homo sapiens, GI266453619, Length=358, Percent_Identity=37.9888268156425, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI11321601, Length=358, Percent_Identity=36.5921787709497, Blast_Score=208, Evalue=6e-54,
Organism=Homo sapiens, GI48762920, Length=311, Percent_Identity=40.1929260450161, Blast_Score=199, Evalue=3e-51,
Organism=Escherichia coli, GI1790350, Length=321, Percent_Identity=70.404984423676, Blast_Score=469, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17558788, Length=320, Percent_Identity=38.4375, Blast_Score=200, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI25147584, Length=317, Percent_Identity=36.9085173501577, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI72003601, Length=289, Percent_Identity=31.8339100346021, Blast_Score=147, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6321679, Length=318, Percent_Identity=38.0503144654088, Blast_Score=198, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6323861, Length=319, Percent_Identity=36.9905956112853, Blast_Score=181, Evalue=1e-46,
Organism=Drosophila melanogaster, GI28573326, Length=315, Percent_Identity=40, Blast_Score=214, Evalue=8e-56,
Organism=Drosophila melanogaster, GI24652337, Length=315, Percent_Identity=39.6825396825397, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI17647809, Length=312, Percent_Identity=40.0641025641026, Blast_Score=205, Evalue=3e-53,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_ACTP2 (A3N1C8)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053819.1
- ProteinModelPortal:   A3N1C8
- SMR:   A3N1C8
- STRING:   A3N1C8
- GeneID:   4849861
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1124
- eggNOG:   COG0205
- HOGENOM:   HBG405044
- OMA:   RCEEFKT
- PhylomeDB:   A3N1C8
- ProtClustDB:   PRK03202
- BioCyc:   APLE416269:APL_1124-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 35515; Mature: 35384

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 132-132 BINDING 167-167 BINDING 248-248 BINDING 254-254 BINDING 257-257

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQIKKIAVLTSGGDAPGMNAAIRGVVRAALNEGLEVYGVQDGYYGLYTDRVIPLDRRS
CCCHHEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCHHH
VSETINRGGTFLGSARFPQFKDPDVRKKSVETLKKYDIDALVVIGGDGSYMGAKLITEEF
HHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHC
GYPCIGIPGTIDNDIVGTDYTIGYQTALETAVEAIDRLRDTSTSHQRISIVEIMGRHCGD
CCCEECCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
LTISAALASGCEYIIVPEKGLDKESLMRNIEDGFNKGKRHAIIAITELMTDVQALAKEIE
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
DRFGHETRATVLGHIQRGGAPCPFDRILASRMGVYAVDLLLQGHGGRCIGIKNENLVHHD
HHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHEEEHEEECCCCEEEEECCCCCHHHH
IIDAINNMRRPFKEELFEAARKLF
HHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKQIKKIAVLTSGGDAPGMNAAIRGVVRAALNEGLEVYGVQDGYYGLYTDRVIPLDRRS
CCHHEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCHHH
VSETINRGGTFLGSARFPQFKDPDVRKKSVETLKKYDIDALVVIGGDGSYMGAKLITEEF
HHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHC
GYPCIGIPGTIDNDIVGTDYTIGYQTALETAVEAIDRLRDTSTSHQRISIVEIMGRHCGD
CCCEECCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
LTISAALASGCEYIIVPEKGLDKESLMRNIEDGFNKGKRHAIIAITELMTDVQALAKEIE
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
DRFGHETRATVLGHIQRGGAPCPFDRILASRMGVYAVDLLLQGHGGRCIGIKNENLVHHD
HHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHEEEHEEECCCCEEEEECCCCCHHHH
IIDAINNMRRPFKEELFEAARKLF
HHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA