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The map label for this gene is rumB [H]

Identifier: 126208584

GI number: 126208584

Start: 1289023

End: 1290198

Strand: Direct

Name: rumB [H]

Synonym: APL_1112

Alternate gene names: 126208584

Gene position: 1289023-1290198 (Clockwise)

Preceding gene: 126208583

Following gene: 126208585

Centisome position: 56.67

GC content: 41.07

Gene sequence:

>1176_bases
ATGATATTAAACTGCCCGCATTTTCAACAACAAGATTGCGTTTCGTGCCAATGGTTAGAAAAGCCGTATGCTACGCAACT
TACCGATAAAGAAATCGATCTTAAGCGGTTAATTTCGCCTTTTATTTTGCAAAATTTCACCGAAATCTTACCGCCTGTTC
AATCTTCACAAAAACAGTTTCGCAATAAAGCCAAAATGGTGGTATCCGGCAGTGTCGAGCGTCCGATTTTAGGCATTTTA
AAAGATCAAACCGATCCGCAAAGTGGTATTGATCTGTGTGATTGCCCACTTTATCCTACTGAATTTGAAGCTCTTTTCCC
GATTCTTAAAGATTTTATCGCCCGAGCTGGACTCGTACCTTATAACATTTCGAAAAAGAAAGGCGAATTGAAGTATATTT
TGATTACGCAAAGCCGATATAACCAATCGATTATGTTGCGTTTTGTGCTAAAAAGCGAGCAAAAACGACCGCTTGTTGAA
CGAGAATTACCGAATCTGTTAGCCAAACTGCCGAAAGATTCGGTGGTAAGCCTGAATATTCAGCCGCAACACGCGGCAAT
TTTAGAGGGCGAAACCGAAATCTTCCTCACCGAAAAAACTACGATTGAAGAAAATTTCAACGATATCCCGTTATTTATCC
GCCCGCAAGGCTTTTTTCAAACCAATCCGAATGTGGCAAGTCAGCTTTATGCCACAGCGCAAAACTGGATCAAAGATTTA
GCGATTCAGCAATTTTGGGATCTGTTCTGTGGTGTGGGTGGCTTTGGTTTACATTGTGCTAAAGCATTACAAGAGAAGAA
TGAGAATGTTCAATTAACCGGTATTGAGATTTCCGCCTCGGCGATTGCCAGTGCCACGAAATCCGCCGAGCAATTACAAC
TTAAAAATATTACATTCGCTTCGCTTGACTCGGCACAATTTGCGCTAAACGAGAAAAGTGCAACACCGGATCTTGTGATA
GTGAATCCGCCTCGCCGTGGTATTGGAAAACCGCTTGCGGAGTTTTTAAACCAATTGGGTACACCTTATTTAATTTATTC
AAGCTGTAATGCCCGAACAATGGCGCAAGATTTTGAAGCCTTAAGCAATTATTCACTCCAAAAGGTGCAATTATTCGATA
TGTTCCCGCATACTTCACATTATGAAGTGCTGACGTTTTTAGTGAAAAAATCGTAA

Upstream 100 bases:

>100_bases
ATAAAGCGCAGCACTGCTAATCAGCCATTTATCTTTTTAGGGTATAAATTATATTTATGCCCTAACAAACCACTAAAAAT
ACCTCGATTCTTTTAACAAA

Downstream 100 bases:

>100_bases
CTTTTTATAGCCCGATATGGGTAGAAGTTTATATTTTTAGTAAAATCTCGGTATAATTAAACCGCTTTAGTTTCATTTAT
CCGTATTCGATCAAAGAAGT

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MILNCPHFQQQDCVSCQWLEKPYATQLTDKEIDLKRLISPFILQNFTEILPPVQSSQKQFRNKAKMVVSGSVERPILGIL
KDQTDPQSGIDLCDCPLYPTEFEALFPILKDFIARAGLVPYNISKKKGELKYILITQSRYNQSIMLRFVLKSEQKRPLVE
RELPNLLAKLPKDSVVSLNIQPQHAAILEGETEIFLTEKTTIEENFNDIPLFIRPQGFFQTNPNVASQLYATAQNWIKDL
AIQQFWDLFCGVGGFGLHCAKALQEKNENVQLTGIEISASAIASATKSAEQLQLKNITFASLDSAQFALNEKSATPDLVI
VNPPRRGIGKPLAEFLNQLGTPYLIYSSCNARTMAQDFEALSNYSLQKVQLFDMFPHTSHYEVLTFLVKKS

Sequences:

>Translated_391_residues
MILNCPHFQQQDCVSCQWLEKPYATQLTDKEIDLKRLISPFILQNFTEILPPVQSSQKQFRNKAKMVVSGSVERPILGIL
KDQTDPQSGIDLCDCPLYPTEFEALFPILKDFIARAGLVPYNISKKKGELKYILITQSRYNQSIMLRFVLKSEQKRPLVE
RELPNLLAKLPKDSVVSLNIQPQHAAILEGETEIFLTEKTTIEENFNDIPLFIRPQGFFQTNPNVASQLYATAQNWIKDL
AIQQFWDLFCGVGGFGLHCAKALQEKNENVQLTGIEISASAIASATKSAEQLQLKNITFASLDSAQFALNEKSATPDLVI
VNPPRRGIGKPLAEFLNQLGTPYLIYSSCNARTMAQDFEALSNYSLQKVQLFDMFPHTSHYEVLTFLVKKS
>Mature_391_residues
MILNCPHFQQQDCVSCQWLEKPYATQLTDKEIDLKRLISPFILQNFTEILPPVQSSQKQFRNKAKMVVSGSVERPILGIL
KDQTDPQSGIDLCDCPLYPTEFEALFPILKDFIARAGLVPYNISKKKGELKYILITQSRYNQSIMLRFVLKSEQKRPLVE
RELPNLLAKLPKDSVVSLNIQPQHAAILEGETEIFLTEKTTIEENFNDIPLFIRPQGFFQTNPNVASQLYATAQNWIKDL
AIQQFWDLFCGVGGFGLHCAKALQEKNENVQLTGIEISASAIASATKSAEQLQLKNITFASLDSAQFALNEKSATPDLVI
VNPPRRGIGKPLAEFLNQLGTPYLIYSSCNARTMAQDFEALSNYSLQKVQLFDMFPHTSHYEVLTFLVKKS

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=429, Percent_Identity=22.3776223776224, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI51173878, Length=429, Percent_Identity=22.3776223776224, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1787083, Length=388, Percent_Identity=54.1237113402062, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1789148, Length=393, Percent_Identity=23.4096692111959, Blast_Score=92, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24666579, Length=383, Percent_Identity=23.4986945169713, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 44153; Mature: 44153

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILNCPHFQQQDCVSCQWLEKPYATQLTDKEIDLKRLISPFILQNFTEILPPVQSSQKQF
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
RNKAKMVVSGSVERPILGILKDQTDPQSGIDLCDCPLYPTEFEALFPILKDFIARAGLVP
HHHHEEEEECCCCCCHHHHHCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCC
YNISKKKGELKYILITQSRYNQSIMLRFVLKSEQKRPLVERELPNLLAKLPKDSVVSLNI
CCCCCCCCCEEEEEEECCCCCCHHHHHEEHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
QPQHAAILEGETEIFLTEKTTIEENFNDIPLFIRPQGFFQTNPNVASQLYATAQNWIKDL
CCCCCEEECCCEEEEEEECCCHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
AIQQFWDLFCGVGGFGLHCAKALQEKNENVQLTGIEISASAIASATKSAEQLQLKNITFA
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHHHECCCEEE
SLDSAQFALNEKSATPDLVIVNPPRRGIGKPLAEFLNQLGTPYLIYSSCNARTMAQDFEA
CCCCHHHEECCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
LSNYSLQKVQLFDMFPHTSHYEVLTFLVKKS
HHCCCCCEEEEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MILNCPHFQQQDCVSCQWLEKPYATQLTDKEIDLKRLISPFILQNFTEILPPVQSSQKQF
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
RNKAKMVVSGSVERPILGILKDQTDPQSGIDLCDCPLYPTEFEALFPILKDFIARAGLVP
HHHHEEEEECCCCCCHHHHHCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCC
YNISKKKGELKYILITQSRYNQSIMLRFVLKSEQKRPLVERELPNLLAKLPKDSVVSLNI
CCCCCCCCCEEEEEEECCCCCCHHHHHEEHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
QPQHAAILEGETEIFLTEKTTIEENFNDIPLFIRPQGFFQTNPNVASQLYATAQNWIKDL
CCCCCEEECCCEEEEEEECCCHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
AIQQFWDLFCGVGGFGLHCAKALQEKNENVQLTGIEISASAIASATKSAEQLQLKNITFA
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHHHECCCEEE
SLDSAQFALNEKSATPDLVIVNPPRRGIGKPLAEFLNQLGTPYLIYSSCNARTMAQDFEA
CCCCHHHEECCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
LSNYSLQKVQLFDMFPHTSHYEVLTFLVKKS
HHCCCCCEEEEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA