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The map label for this gene is plsB

Identifier: 126208579

GI number: 126208579

Start: 1282970

End: 1285444

Strand: Reverse

Name: plsB

Synonym: APL_1107

Alternate gene names: 126208579

Gene position: 1285444-1282970 (Counterclockwise)

Preceding gene: 126208588

Following gene: 126208578

Centisome position: 56.52

GC content: 42.75

Gene sequence:

>2475_bases
ATGTCTAGCCTCTTAAATTTTTATCGTAAAGTATTAAATGTTCCGCTTTCACTGTTAGTTAAATCTCGTGCTATCCCGAC
CGATCCGGTCAAAGAGCTTAACTTAAATTTGGAACAGCCCATTATTTATGTTCTACCTTACACTTCTCAAACCGATTTAT
TGATTCTCCAAAAAAACTGCTTGTCGCTTAATTTACCCGATCCGTTACAGAATAACGAATTAAACGGTCAATCTTTACCT
CGTTATGTTTTCTTAGACGAGGGACGCCGCTTTTTTAAGTCTAAAGGTGCAAAGAGCGAAACGGAGTCCATCTTTTATCG
TTATTTGGATTTACATCGCAATAATGAATCGTTGGACGTGCAATTGATACCGGCTTCGGTATTGTGGGGACGTTCTCCGG
GGAAAGAATCCGAACCGCATTTACGCTTAATGAGCAGTTTCCAACGCATTATTTCGATGATTTGGTTCGGGCGTGATAAT
TTTGTGCGCTTTTCTCAGGCGTTGTCGTTGAAATATATGGTAGCGGAACACGGTGCCGATGAAGGCATCGCTCAAAAACT
CGCACGAGTGGCAAAAATCCATTTTGCGAAGCAACGTTATTCCGCAATGGGGCCTAGATTGCCTGATCGCCAAGCGATGT
TTAATAAAATCATTCAATCGCCGGCGATTAAAGTGGCAATAGAAGAAGAAGCGAAAACGAAGAAAATTTCAATTGAAAAA
GCGCGCCAAGAAGCGGAAAAGATCGTGAATGAGATTGCGGCGGACGTTAGCCATGAGAGTTTACGCATTGCCGATCGCGT
ATTGAGCTGGTTATGGAATAAGTTGTACCAGGGGATCAACGTTCAAAACGGGGATCGCGTACGTAAATTGGCGTTAGAAG
GACATGAAATCGTTTATGTACCTTGTCACCGTAGCCATATGGACTATTTATTGCTTTCGTATTTGCTTTATCATCAAGGA
TTGGTACCGCCGCATATCGCAGCGGGGATTAACCTGAATTTCTTTCCGGCAGGACCGATTTTCCGTAGCTGGGGCGCATT
TTTTATTCGCCGTACTTTTAAAGGAAACCGCTTATATTCGACGATTTTCCGCGAATATTTGGCGGAGCTTTTCTATCGTG
GCTATTCGGTGGAGTATTTTATCGAAGGCGGTCGTTCCCGTACCGGACGTTTACTTGAGCCGAAAACCGGTATGATGTCG
ATGACTTTACAAGCTCTGCAGCGCGGCTTAACCCGTCCGATTAGCATCGTGCCGGTGTATATCGGTTATGAACACGTGTT
GGAAGTGGATACTTATGCGAAAGAGTTGCGCGGTGCGGAAAAAGAGAAAGAAAACGCCGGCTTAGTTTTACGCGTGATCA
AGAAACTGAAAAACTTAGGACAATGCTATGTGAACTTTGCCGAGCCTATTCAGGTTAATAATTATTTAAATCAGCATTTT
CCGGAATGGAAAGAATCTCAAGCTGAAGACAGTCGTCCGAAATGGTTGAATGAAGCGGTGGATTCGGTTGCACATCAAGT
CATGATCAACATTAATAAAGCTGCGGCGATTAATGCGAAAAACTTAATCGGTTCTGTATTGCTTGCCTCTCGCCAACGTG
CATTGGCTCGCGAACAATTGATCGAACAAGTCGATAGCTATTTGCAGCTGTTTAAAAACGTCAGTTATTCCGACGATGCT
ATCGTACCGAACGACAACGCCGAAGAAATGCTTAATCATGTGCTGACTTTACCTCGTTCCGGTGTAATTAGTGAGAAAGA
CAGTTTCGGTGAAATGATTCGTCTGGATCGAGAATCGGCAGTCTTGATGACGTATTATCGTAATAATATTCAGCATCTAT
TCGTACTGCCTTCACTGGTCGCAAGCATTATTTTACATCATGAATCGGTCTCGAAAGATCTGATTATTAAAACGGTTAAT
CGTATTTATCCGTTCTTAAAAGCGGAGCTGTTTCTTCATTTTGAAGAGAACGATGTGCGAAACCAAGTGGAAGCGATTTT
AACGGAATTCTCCGCACAACGTATCGTCAAATATGAGAGCGACGTATTACAAATTAATTGCGTACGCGTTCGAGCGTTAC
AACTGCATGCGGCGGGAGTACGTGAGATTCTGCAACGTTACTATATCAGCTTGAGTATTTTGCTTGAACATCCGGAAATT
AGCCGTGCCGCATTGGAAAAAGAGAGCCGTTCTATCGCACAACGATTATCTATCTTACACGGTATTAATGCACCGGAGTT
TTTTGATAAAGCGCTGTTTTCAACCTTTAGCGCCAGTTTAAAAGCGCAAGGTTATTTTGATAGCGAAGGAAATTGCATAC
TTGAAAAAGCGAAAGAAGCGGAGGAAATATTACGTTCACTGATTTCGGTTGAAGTTCAACTGACCATTCAAGGGGCAATG
GAAAAAGTGGAAGAAGTTGAAAATACTGAAACGGTAGTCAAAACAGCGGAAGCCGTAACGGAAAAGAACGAGTAA

Upstream 100 bases:

>100_bases
ATTTGTAACTAGATAAATTCAGAAAATCCTCACAAATTTATGGTAAACTGCGGACGAAATACATTAGACGGCATAAACCA
TAACAATTAAGGATATTAAA

Downstream 100 bases:

>100_bases
ACTTTTAAATATCCGATACTTAAAAAGAACAAGCGGTAAGATTTTGCAAAATTTTTACAAAATCTTACCGCTTTATTATA
TAATCTGCGCAAACGTTTGC

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT

Number of amino acids: Translated: 824; Mature: 823

Protein sequence:

>824_residues
MSSLLNFYRKVLNVPLSLLVKSRAIPTDPVKELNLNLEQPIIYVLPYTSQTDLLILQKNCLSLNLPDPLQNNELNGQSLP
RYVFLDEGRRFFKSKGAKSETESIFYRYLDLHRNNESLDVQLIPASVLWGRSPGKESEPHLRLMSSFQRIISMIWFGRDN
FVRFSQALSLKYMVAEHGADEGIAQKLARVAKIHFAKQRYSAMGPRLPDRQAMFNKIIQSPAIKVAIEEEAKTKKISIEK
ARQEAEKIVNEIAADVSHESLRIADRVLSWLWNKLYQGINVQNGDRVRKLALEGHEIVYVPCHRSHMDYLLLSYLLYHQG
LVPPHIAAGINLNFFPAGPIFRSWGAFFIRRTFKGNRLYSTIFREYLAELFYRGYSVEYFIEGGRSRTGRLLEPKTGMMS
MTLQALQRGLTRPISIVPVYIGYEHVLEVDTYAKELRGAEKEKENAGLVLRVIKKLKNLGQCYVNFAEPIQVNNYLNQHF
PEWKESQAEDSRPKWLNEAVDSVAHQVMININKAAAINAKNLIGSVLLASRQRALAREQLIEQVDSYLQLFKNVSYSDDA
IVPNDNAEEMLNHVLTLPRSGVISEKDSFGEMIRLDRESAVLMTYYRNNIQHLFVLPSLVASIILHHESVSKDLIIKTVN
RIYPFLKAELFLHFEENDVRNQVEAILTEFSAQRIVKYESDVLQINCVRVRALQLHAAGVREILQRYYISLSILLEHPEI
SRAALEKESRSIAQRLSILHGINAPEFFDKALFSTFSASLKAQGYFDSEGNCILEKAKEAEEILRSLISVEVQLTIQGAM
EKVEEVENTETVVKTAEAVTEKNE

Sequences:

>Translated_824_residues
MSSLLNFYRKVLNVPLSLLVKSRAIPTDPVKELNLNLEQPIIYVLPYTSQTDLLILQKNCLSLNLPDPLQNNELNGQSLP
RYVFLDEGRRFFKSKGAKSETESIFYRYLDLHRNNESLDVQLIPASVLWGRSPGKESEPHLRLMSSFQRIISMIWFGRDN
FVRFSQALSLKYMVAEHGADEGIAQKLARVAKIHFAKQRYSAMGPRLPDRQAMFNKIIQSPAIKVAIEEEAKTKKISIEK
ARQEAEKIVNEIAADVSHESLRIADRVLSWLWNKLYQGINVQNGDRVRKLALEGHEIVYVPCHRSHMDYLLLSYLLYHQG
LVPPHIAAGINLNFFPAGPIFRSWGAFFIRRTFKGNRLYSTIFREYLAELFYRGYSVEYFIEGGRSRTGRLLEPKTGMMS
MTLQALQRGLTRPISIVPVYIGYEHVLEVDTYAKELRGAEKEKENAGLVLRVIKKLKNLGQCYVNFAEPIQVNNYLNQHF
PEWKESQAEDSRPKWLNEAVDSVAHQVMININKAAAINAKNLIGSVLLASRQRALAREQLIEQVDSYLQLFKNVSYSDDA
IVPNDNAEEMLNHVLTLPRSGVISEKDSFGEMIRLDRESAVLMTYYRNNIQHLFVLPSLVASIILHHESVSKDLIIKTVN
RIYPFLKAELFLHFEENDVRNQVEAILTEFSAQRIVKYESDVLQINCVRVRALQLHAAGVREILQRYYISLSILLEHPEI
SRAALEKESRSIAQRLSILHGINAPEFFDKALFSTFSASLKAQGYFDSEGNCILEKAKEAEEILRSLISVEVQLTIQGAM
EKVEEVENTETVVKTAEAVTEKNE
>Mature_823_residues
SSLLNFYRKVLNVPLSLLVKSRAIPTDPVKELNLNLEQPIIYVLPYTSQTDLLILQKNCLSLNLPDPLQNNELNGQSLPR
YVFLDEGRRFFKSKGAKSETESIFYRYLDLHRNNESLDVQLIPASVLWGRSPGKESEPHLRLMSSFQRIISMIWFGRDNF
VRFSQALSLKYMVAEHGADEGIAQKLARVAKIHFAKQRYSAMGPRLPDRQAMFNKIIQSPAIKVAIEEEAKTKKISIEKA
RQEAEKIVNEIAADVSHESLRIADRVLSWLWNKLYQGINVQNGDRVRKLALEGHEIVYVPCHRSHMDYLLLSYLLYHQGL
VPPHIAAGINLNFFPAGPIFRSWGAFFIRRTFKGNRLYSTIFREYLAELFYRGYSVEYFIEGGRSRTGRLLEPKTGMMSM
TLQALQRGLTRPISIVPVYIGYEHVLEVDTYAKELRGAEKEKENAGLVLRVIKKLKNLGQCYVNFAEPIQVNNYLNQHFP
EWKESQAEDSRPKWLNEAVDSVAHQVMININKAAAINAKNLIGSVLLASRQRALAREQLIEQVDSYLQLFKNVSYSDDAI
VPNDNAEEMLNHVLTLPRSGVISEKDSFGEMIRLDRESAVLMTYYRNNIQHLFVLPSLVASIILHHESVSKDLIIKTVNR
IYPFLKAELFLHFEENDVRNQVEAILTEFSAQRIVKYESDVLQINCVRVRALQLHAAGVREILQRYYISLSILLEHPEIS
RAALEKESRSIAQRLSILHGINAPEFFDKALFSTFSASLKAQGYFDSEGNCILEKAKEAEEILRSLISVEVQLTIQGAME
KVEEVENTETVVKTAEAVTEKNE

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family

Homologues:

Organism=Homo sapiens, GI7657134, Length=478, Percent_Identity=28.6610878661088, Blast_Score=172, Evalue=9e-43,
Organism=Homo sapiens, GI190358539, Length=421, Percent_Identity=25.8907363420428, Blast_Score=143, Evalue=6e-34,
Organism=Escherichia coli, GI87082362, Length=806, Percent_Identity=56.6997518610422, Blast_Score=954, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147672, Length=312, Percent_Identity=33.3333333333333, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71988723, Length=250, Percent_Identity=32.8, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71988728, Length=190, Percent_Identity=38.4210526315789, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI17864692, Length=357, Percent_Identity=29.4117647058824, Blast_Score=157, Evalue=4e-38,
Organism=Drosophila melanogaster, GI21357731, Length=485, Percent_Identity=25.5670103092783, Blast_Score=136, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24650754, Length=485, Percent_Identity=25.5670103092783, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24650752, Length=485, Percent_Identity=25.5670103092783, Blast_Score=135, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLSB_ACTP2 (A3N1B3)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053804.1
- ProteinModelPortal:   A3N1B3
- STRING:   A3N1B3
- GeneID:   4849349
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1107
- eggNOG:   COG2937
- HOGENOM:   HBG296590
- OMA:   VIYVPCH
- ProtClustDB:   PRK04974
- BioCyc:   APLE416269:APL_1107-MONOMER
- HAMAP:   MF_00393
- InterPro:   IPR002123
- InterPro:   IPR022284
- SMART:   SM00563
- TIGRFAMs:   TIGR03703

Pfam domain/function: PF01553 Acyltransferase

EC number: =2.3.1.15

Molecular weight: Translated: 94246; Mature: 94115

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLLNFYRKVLNVPLSLLVKSRAIPTDPVKELNLNLEQPIIYVLPYTSQTDLLILQKNC
CCHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHCCCCCCCCEEEEEECCCCCCEEEEECCC
LSLNLPDPLQNNELNGQSLPRYVFLDEGRRFFKSKGAKSETESIFYRYLDLHRNNESLDV
CCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEE
QLIPASVLWGRSPGKESEPHLRLMSSFQRIISMIWFGRDNFVRFSQALSLKYMVAEHGAD
EEECHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
EGIAQKLARVAKIHFAKQRYSAMGPRLPDRQAMFNKIIQSPAIKVAIEEEAKTKKISIEK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
ARQEAEKIVNEIAADVSHESLRIADRVLSWLWNKLYQGINVQNGDRVRKLALEGHEIVYV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEE
PCHRSHMDYLLLSYLLYHQGLVPPHIAAGINLNFFPAGPIFRSWGAFFIRRTFKGNRLYS
CCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHH
TIFREYLAELFYRGYSVEYFIEGGRSRTGRLLEPKTGMMSMTLQALQRGLTRPISIVPVY
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEE
IGYEHVLEVDTYAKELRGAEKEKENAGLVLRVIKKLKNLGQCYVNFAEPIQVNNYLNQHF
ECHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
PEWKESQAEDSRPKWLNEAVDSVAHQVMININKAAAINAKNLIGSVLLASRQRALAREQL
CCHHHCCCCCCCHHHHHHHHHHHHHHHHEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHH
IEQVDSYLQLFKNVSYSDDAIVPNDNAEEMLNHVLTLPRSGVISEKDSFGEMIRLDRESA
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCC
VLMTYYRNNIQHLFVLPSLVASIILHHESVSKDLIIKTVNRIYPFLKAELFLHFEENDVR
EEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCHHHH
NQVEAILTEFSAQRIVKYESDVLQINCVRVRALQLHAAGVREILQRYYISLSILLEHPEI
HHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHEEEECCCH
SRAALEKESRSIAQRLSILHGINAPEFFDKALFSTFSASLKAQGYFDSEGNCILEKAKEA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHH
EEILRSLISVEVQLTIQGAMEKVEEVENTETVVKTAEAVTEKNE
HHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSLLNFYRKVLNVPLSLLVKSRAIPTDPVKELNLNLEQPIIYVLPYTSQTDLLILQKNC
CHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHCCCCCCCCEEEEEECCCCCCEEEEECCC
LSLNLPDPLQNNELNGQSLPRYVFLDEGRRFFKSKGAKSETESIFYRYLDLHRNNESLDV
CCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEE
QLIPASVLWGRSPGKESEPHLRLMSSFQRIISMIWFGRDNFVRFSQALSLKYMVAEHGAD
EEECHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
EGIAQKLARVAKIHFAKQRYSAMGPRLPDRQAMFNKIIQSPAIKVAIEEEAKTKKISIEK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
ARQEAEKIVNEIAADVSHESLRIADRVLSWLWNKLYQGINVQNGDRVRKLALEGHEIVYV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEE
PCHRSHMDYLLLSYLLYHQGLVPPHIAAGINLNFFPAGPIFRSWGAFFIRRTFKGNRLYS
CCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHH
TIFREYLAELFYRGYSVEYFIEGGRSRTGRLLEPKTGMMSMTLQALQRGLTRPISIVPVY
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEE
IGYEHVLEVDTYAKELRGAEKEKENAGLVLRVIKKLKNLGQCYVNFAEPIQVNNYLNQHF
ECHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
PEWKESQAEDSRPKWLNEAVDSVAHQVMININKAAAINAKNLIGSVLLASRQRALAREQL
CCHHHCCCCCCCHHHHHHHHHHHHHHHHEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHH
IEQVDSYLQLFKNVSYSDDAIVPNDNAEEMLNHVLTLPRSGVISEKDSFGEMIRLDRESA
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCC
VLMTYYRNNIQHLFVLPSLVASIILHHESVSKDLIIKTVNRIYPFLKAELFLHFEENDVR
EEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCHHHH
NQVEAILTEFSAQRIVKYESDVLQINCVRVRALQLHAAGVREILQRYYISLSILLEHPEI
HHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHEEEECCCH
SRAALEKESRSIAQRLSILHGINAPEFFDKALFSTFSASLKAQGYFDSEGNCILEKAKEA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHH
EEILRSLISVEVQLTIQGAMEKVEEVENTETVVKTAEAVTEKNE
HHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA