Click here to switch to the map view.

The map label for this gene is purT

Identifier: 126208578

GI number: 126208578

Start: 1281666

End: 1282847

Strand: Reverse

Name: purT

Synonym: APL_1106

Alternate gene names: 126208578

Gene position: 1282847-1281666 (Counterclockwise)

Preceding gene: 126208579

Following gene: 126208573

Centisome position: 56.4

GC content: 45.6

Gene sequence:

>1182_bases
ATGACCACAATTGGCACACCGTTAAGACCGAATGCTACAAAAGTAATGATGCTTGGCTCGGGTGAGTTAGGTAAAGAAGT
TGTTATCGAATTACAACGCTTAGGAGTAGAAGTGATCGCGGTCGATCGCTATGAAAATGCACCGGCGCAGCAAGTGGCAC
ATCGAGCTTATACGATCTCTATGTTAGATGGCGCAGCATTACGAGCATTAGTGGAAAAAGAAAAGCCGGATTTTATTGTG
CCGGAAGTTGAAGCGATTGCAACGGCAACCTTGGTAGAGTTAGAGCAAGAAGGTTATAACGTGGTACCGACCGCCAAAGC
AACCCAATTGACGATGAACCGCGAAGGAATTCGTCGTTTAGCGGCGGAAGAACTCGGTTTAAAAACATCGCCTTACCGTT
TTGTAGATAATCTGGAAGATTTTAAACAAGCGGTTGCCGAGATTGGTATTCCGTGTGTGGTTAAACCGATTATGTCGTCA
TCCGGTCACGGGCAATCGGTGATTAAATCGGAAGATCAAATTCAACAAGCGTGGGATTATTCACAAGAAGGCGGACGTGC
CGGCGGCGGACGAGTAATTGTGGAGGGCTTTATCAAATTCGATTATGAAATCACGCAATTAACCGTGCGTCACGTAAACG
GTACTTCTTTCTTAGCACCAATCGGGCATCGCCAAGAAGACGGCGATTATCGTGAATCTTGGCAGCCGCAAGCGATGTCC
GACCTAGCGTTAAAACGTGCGCAAGAAACCGCAGAGCGAATTACAACCGCATTAGGCGGACGTGGTATTTTCGGTGTGGA
ATTATTTGTGTGTGGTGATGAAATTATCTTTAATGAAGTTTCGCCTCGCCCGCATGATACGGGAATGGTCACCATGGCTT
CGCAAGAGCTTTCTCAGTTTGCCTTACACGCTCGTGCGATTTTAGGCTTACCGATTCCGGAAATTTATCAAATTAGCCCG
GCGGCTTCAAAAGCGATTGTAGTAGAAGGTAAATCAAACAATATGACTTTCGGTAATCTTGATAAAGTGTTGGAAGAAAT
CGGTACTAATATTCGTTTATTCGGCAAAGGCGAAGTAAACGGTCACCGTCGTTTAGGAGTTATCTTAGCTCGTGATGAAA
ATACCGAAAAAGCCTTAGCGAAAGCCGAACGTGCCTATGCGAAATTAGCGGTACAGCTTTAA

Upstream 100 bases:

>100_bases
AAAAGAACAAGCGGTAAGATTTTGCAAAATTTTTACAAAATCTTACCGCTTTATTATATAATCTGCGCAAACGTTTGCTT
TAACTTTAGAGGAAGAAAAA

Downstream 100 bases:

>100_bases
TCCCTTCTTTTTTATGAAAATACTAGTGGATAAACCGCTAGTATTTTTTATTCAATTCTTCTTATTAATTCACTTATTAT
TTAATATTCTTCCTATTTCC

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTTIGTPLRPNATKVMMLGSGELGKEVVIELQRLGVEVIAVDRYENAPAQQVAHRAYTISMLDGAALRALVEKEKPDFIV
PEVEAIATATLVELEQEGYNVVPTAKATQLTMNREGIRRLAAEELGLKTSPYRFVDNLEDFKQAVAEIGIPCVVKPIMSS
SGHGQSVIKSEDQIQQAWDYSQEGGRAGGGRVIVEGFIKFDYEITQLTVRHVNGTSFLAPIGHRQEDGDYRESWQPQAMS
DLALKRAQETAERITTALGGRGIFGVELFVCGDEIIFNEVSPRPHDTGMVTMASQELSQFALHARAILGLPIPEIYQISP
AASKAIVVEGKSNNMTFGNLDKVLEEIGTNIRLFGKGEVNGHRRLGVILARDENTEKALAKAERAYAKLAVQL

Sequences:

>Translated_393_residues
MTTIGTPLRPNATKVMMLGSGELGKEVVIELQRLGVEVIAVDRYENAPAQQVAHRAYTISMLDGAALRALVEKEKPDFIV
PEVEAIATATLVELEQEGYNVVPTAKATQLTMNREGIRRLAAEELGLKTSPYRFVDNLEDFKQAVAEIGIPCVVKPIMSS
SGHGQSVIKSEDQIQQAWDYSQEGGRAGGGRVIVEGFIKFDYEITQLTVRHVNGTSFLAPIGHRQEDGDYRESWQPQAMS
DLALKRAQETAERITTALGGRGIFGVELFVCGDEIIFNEVSPRPHDTGMVTMASQELSQFALHARAILGLPIPEIYQISP
AASKAIVVEGKSNNMTFGNLDKVLEEIGTNIRLFGKGEVNGHRRLGVILARDENTEKALAKAERAYAKLAVQL
>Mature_392_residues
TTIGTPLRPNATKVMMLGSGELGKEVVIELQRLGVEVIAVDRYENAPAQQVAHRAYTISMLDGAALRALVEKEKPDFIVP
EVEAIATATLVELEQEGYNVVPTAKATQLTMNREGIRRLAAEELGLKTSPYRFVDNLEDFKQAVAEIGIPCVVKPIMSSS
GHGQSVIKSEDQIQQAWDYSQEGGRAGGGRVIVEGFIKFDYEITQLTVRHVNGTSFLAPIGHRQEDGDYRESWQPQAMSD
LALKRAQETAERITTALGGRGIFGVELFVCGDEIIFNEVSPRPHDTGMVTMASQELSQFALHARAILGLPIPEIYQISPA
ASKAIVVEGKSNNMTFGNLDKVLEEIGTNIRLFGKGEVNGHRRLGVILARDENTEKALAKAERAYAKLAVQL

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=394, Percent_Identity=65.4822335025381, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI1786733, Length=263, Percent_Identity=25.0950570342205, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324702, Length=373, Percent_Identity=26.0053619302949, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_ACTP2 (A3N1B2)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053803.1
- ProteinModelPortal:   A3N1B2
- SMR:   A3N1B2
- STRING:   A3N1B2
- GeneID:   4850260
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1106
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- PhylomeDB:   A3N1B2
- ProtClustDB:   PRK09288
- BioCyc:   APLE416269:APL_1106-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42970; Mature: 42838

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 82-82 BINDING 114-114 BINDING 155-155 BINDING 203-203 BINDING 267-267 BINDING 279-279 BINDING 286-286 BINDING 356-356

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIGTPLRPNATKVMMLGSGELGKEVVIELQRLGVEVIAVDRYENAPAQQVAHRAYTIS
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHEEEE
MLDGAALRALVEKEKPDFIVPEVEAIATATLVELEQEGYNVVPTAKATQLTMNREGIRRL
ECCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCCEEECCCCCEEEEECHHHHHHH
AAEELGLKTSPYRFVDNLEDFKQAVAEIGIPCVVKPIMSSSGHGQSVIKSEDQIQQAWDY
HHHHHCCCCCCHHHHHCHHHHHHHHHHCCCCEEEHHHHCCCCCCCHHHCCHHHHHHHHCC
SQEGGRAGGGRVIVEGFIKFDYEITQLTVRHVNGTSFLAPIGHRQEDGDYRESWQPQAMS
CCCCCCCCCCEEEEEEEEECCEEEEEEEEEEECCCEEECCCCCCCCCCCHHHCCCCHHHH
DLALKRAQETAERITTALGGRGIFGVELFVCGDEIIFNEVSPRPHDTGMVTMASQELSQF
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHEEECCCCCCCCCCEEEEHHHHHHHH
ALHARAILGLPIPEIYQISPAASKAIVVEGKSNNMTFGNLDKVLEEIGTNIRLFGKGEVN
HHHHHHHHCCCCCHHEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCCC
GHRRLGVILARDENTEKALAKAERAYAKLAVQL
CCEEEEEEEECCCCHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
TTIGTPLRPNATKVMMLGSGELGKEVVIELQRLGVEVIAVDRYENAPAQQVAHRAYTIS
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHEEEE
MLDGAALRALVEKEKPDFIVPEVEAIATATLVELEQEGYNVVPTAKATQLTMNREGIRRL
ECCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCCEEECCCCCEEEEECHHHHHHH
AAEELGLKTSPYRFVDNLEDFKQAVAEIGIPCVVKPIMSSSGHGQSVIKSEDQIQQAWDY
HHHHHCCCCCCHHHHHCHHHHHHHHHHCCCCEEEHHHHCCCCCCCHHHCCHHHHHHHHCC
SQEGGRAGGGRVIVEGFIKFDYEITQLTVRHVNGTSFLAPIGHRQEDGDYRESWQPQAMS
CCCCCCCCCCEEEEEEEEECCEEEEEEEEEEECCCEEECCCCCCCCCCCHHHCCCCHHHH
DLALKRAQETAERITTALGGRGIFGVELFVCGDEIIFNEVSPRPHDTGMVTMASQELSQF
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHEEECCCCCCCCCCEEEEHHHHHHHH
ALHARAILGLPIPEIYQISPAASKAIVVEGKSNNMTFGNLDKVLEEIGTNIRLFGKGEVN
HHHHHHHHCCCCCHHEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCCC
GHRRLGVILARDENTEKALAKAERAYAKLAVQL
CCEEEEEEEECCCCHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA