Click here to switch to the map view.

The map label for this gene is clpB [H]

Identifier: 126208559

GI number: 126208559

Start: 1257302

End: 1259875

Strand: Direct

Name: clpB [H]

Synonym: APL_1087

Alternate gene names: 126208559

Gene position: 1257302-1259875 (Clockwise)

Preceding gene: 126208557

Following gene: 126208566

Centisome position: 55.28

GC content: 44.64

Gene sequence:

>2574_bases
ATGAATTTCGAAAAATTTACTACCAAATTGCAAGAAGCCCTTGCAGAAGCACAATCGTTAGCTATCGGAAAAGATAATAA
CTATATTGAACCGGCACATTTACTTTATGCGTTAGTCAAACAACCGGACGGCTCTATCGCCCCGCTATTTACTGCACTTA
ATGTACAACCGCAACGTATTTTGAGTGAATTAGAGACGATTTTAAACCGCTTACCGACCGTATCCGGCGGTACAACCCAA
CCGTCACAACAATTAATTCGCCTGTTAAATCAATGCGATAAACTAGCGCAGCAATTCGGTGACAGCTTTATTTCATCGGA
ATTATTCGTGCTTGCCGCACTGGACGATAACGGCGATTTAGGCAAATTATTAAAACAATTTGGCTTGACTAAAGAAAAAG
TAAACGCAGCCATCAGCCAAATTCGAGGAGGAGAAAACGTGAATAATCAAAACGCAGAAGAAACCAGACAGGCCCTTCAA
AAATATACTATCGATCTCACAGAAAGAGCCAAAGCCGGTAAATTAGATCCGGTCATCGGGCGTGATGAAGAAATCCGCCG
TGCAGTACAAGTTTTACAACGCCGTACTAAAAATAACCCGGTATTAATCGGTGAACCTGGGGTCGGTAAAACTGCGATCG
TAGAAGGCTTGGCACAACGTATCGTCAACGGCGAAGTGCCGGAAGGTTTAAAAAATAAACGTGTACTTTCTCTCGATATG
GGCGCATTAATTGCTGGTGCAAAATATCGCGGCGAATTTGAAGAACGTCTAAAAGCGGTACTTAACGAATTAGCCAAAGA
AGAAGGTCAGGTTATCTTATTTATTGATGAAATTCACACCATGGTCGGTGCGGGTAAAACAGACGGCGCAATGGATGCCG
GTAACTTACTCAAGCCGTCACTTGCTCGCGGCGAATTACATTGCGTTGGGGCAACTACGTTAGACGAATACCGCCAATAT
ATCGAAAAAGATGCGGCACTTGAACGTCGTTTCCAAAAAGTATTAGTGGACGAACCGACCGTTGAGGATACGATTGCAAT
TTTACGCGGCTTGAAAGAACGTTATGAAATTCATCACCACGTACAAATTACCGACCCGGCAATTGTCGCGGCGGCAACGC
TTTCGCACCGTTATATTTCCGACCGCCAATTACCGGATAAAGCGATCGATTTAATTGATGAAGCCGCATCCAGTTTACGT
ATGGAAATCGATTCTAAACCTGAGCCGTTGGATAAACTTGAACGCCGTATTATTCAACTTAAATTGGAACGCCAAGCGTT
ACAAAAAGAGGAAGATGAAGCAAGCCGCCAACGTCTTGCAAAATTAGATGAAGAATTGACCGCTCGCGAGCGTGAATATT
CCGAATTGGAAGAAGTTTGGAAAGCGGAGAAATCAGCATTACTCGGTACGCAACATATCAAAACCGAGTTAGAAAATGCC
CGTATCGAAATGGATCAGGCTCGCCGTGAAAATAATTTCGAGAAAATGTCCGAATTGCAATACGGTAAAATTCCGGCATT
GGAAAAACAATTACACGAAGCGGTAAAACGCGAGGAAGAAGGCAGTGAAAATCAACTGCTTCGTACCAAAGTCACCGAGG
AGGAAATCGCAGAAGTGCTTTCAAAAGCGACCGGTATTCCGGTTTCCAAAATGATGGAAGGCGAAAAAGAAAAACTATTG
CGTATGGAAGAAGTGTTACACAGCCGTGTCATCGGACAAAATGAAGCGGTAGAAGCGGTGGCAAATGCAATTCGCCGTAG
CCGTGCCGGATTGTCGGATCCGAATCGTCCGATCGGTTCGTTCTTATTCCTTGGCCCGACCGGGGTCGGTAAGACCGAAC
TTTGCAAAACCTTGGCGAATTTCCTATTCGATGATCCTGATGCGATGGTACGTATCGATATGTCGGAATTTATGGAAAAA
CACAGCGTTTCTCGTTTAGTCGGCGCACCTCCGGGCTATGTCGGTTATGAAGAAGGCGGTTATTTAACCGAAGCGGCTCG
CCGCCGTCCATATTCGGTAGTATTACTCGATGAAGTGGAAAAAGCGCACCCTGACGTGTTTAATATCTTGCTTCAAGTGT
TGGATGACGGTCGTTTAACCGACGGTCAAGGTCGTACGGTGGATTTTCGTAATACGGTAGTAATTATGACTTCAAACTTA
GGTTCACATTTAATCCAAGAAAATCCGGAATTAGATTATGCTGGAATGAAAGAAGTAGTGATGTCAGTGGTAGGACAACA
TTTCCGTCCTGAATTTATCAACCGTATTGATGAAACTGTGGTGTTCCACCCACTTGGTCAAGCACATATCCGTGCGATTG
CTCGCATTCAGTTACAACGTTTAATCGCTCGCCTTGCGGAACGCGGTTACGAAGTAACAGTTACCGATGCGGCATTAGAC
CATATCGGCAAAGCCGGCTTTGACCCGTTATTCGGTGCAAGACCGCTCAAACGTGCGATTCAGCAAGAGCTTGAAAATCC
GCTTTCACAACAGATCTTAAGCGGCAAATTATTACCGAACAGCCCAGTTGTGGTGGACTATCAAAATGACCGATTAAGCA
TTAGCAACAGCTAA

Upstream 100 bases:

>100_bases
CATTTTTACTTGAAATAATCTTGACTAGCTCCATTTATTAAAACGTTGAAGCGGTATAAATTCTGCTCGATACAAATTAA
TGAAATTTAGGAGTAAAAAT

Downstream 100 bases:

>100_bases
TTCCATAAACAAGCGGTCGAGTTTGCAGAAATTTTGCAAATTCGACCGCTTTTCTTTTAGGCGTTATGGCGATTAATCCA
CCAAACTAACGACAGCATAA

Product: chaperone ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MNFEKFTTKLQEALAEAQSLAIGKDNNYIEPAHLLYALVKQPDGSIAPLFTALNVQPQRILSELETILNRLPTVSGGTTQ
PSQQLIRLLNQCDKLAQQFGDSFISSELFVLAALDDNGDLGKLLKQFGLTKEKVNAAISQIRGGENVNNQNAEETRQALQ
KYTIDLTERAKAGKLDPVIGRDEEIRRAVQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKNKRVLSLDM
GALIAGAKYRGEFEERLKAVLNELAKEEGQVILFIDEIHTMVGAGKTDGAMDAGNLLKPSLARGELHCVGATTLDEYRQY
IEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYEIHHHVQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAASSLR
MEIDSKPEPLDKLERRIIQLKLERQALQKEEDEASRQRLAKLDEELTAREREYSELEEVWKAEKSALLGTQHIKTELENA
RIEMDQARRENNFEKMSELQYGKIPALEKQLHEAVKREEEGSENQLLRTKVTEEEIAEVLSKATGIPVSKMMEGEKEKLL
RMEEVLHSRVIGQNEAVEAVANAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKTLANFLFDDPDAMVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAARRRPYSVVLLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSHLIQENPELDYAGMKEVVMSVVGQHFRPEFINRIDETVVFHPLGQAHIRAIARIQLQRLIARLAERGYEVTVTDAALD
HIGKAGFDPLFGARPLKRAIQQELENPLSQQILSGKLLPNSPVVVDYQNDRLSISNS

Sequences:

>Translated_857_residues
MNFEKFTTKLQEALAEAQSLAIGKDNNYIEPAHLLYALVKQPDGSIAPLFTALNVQPQRILSELETILNRLPTVSGGTTQ
PSQQLIRLLNQCDKLAQQFGDSFISSELFVLAALDDNGDLGKLLKQFGLTKEKVNAAISQIRGGENVNNQNAEETRQALQ
KYTIDLTERAKAGKLDPVIGRDEEIRRAVQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKNKRVLSLDM
GALIAGAKYRGEFEERLKAVLNELAKEEGQVILFIDEIHTMVGAGKTDGAMDAGNLLKPSLARGELHCVGATTLDEYRQY
IEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYEIHHHVQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAASSLR
MEIDSKPEPLDKLERRIIQLKLERQALQKEEDEASRQRLAKLDEELTAREREYSELEEVWKAEKSALLGTQHIKTELENA
RIEMDQARRENNFEKMSELQYGKIPALEKQLHEAVKREEEGSENQLLRTKVTEEEIAEVLSKATGIPVSKMMEGEKEKLL
RMEEVLHSRVIGQNEAVEAVANAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKTLANFLFDDPDAMVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAARRRPYSVVLLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSHLIQENPELDYAGMKEVVMSVVGQHFRPEFINRIDETVVFHPLGQAHIRAIARIQLQRLIARLAERGYEVTVTDAALD
HIGKAGFDPLFGARPLKRAIQQELENPLSQQILSGKLLPNSPVVVDYQNDRLSISNS
>Mature_857_residues
MNFEKFTTKLQEALAEAQSLAIGKDNNYIEPAHLLYALVKQPDGSIAPLFTALNVQPQRILSELETILNRLPTVSGGTTQ
PSQQLIRLLNQCDKLAQQFGDSFISSELFVLAALDDNGDLGKLLKQFGLTKEKVNAAISQIRGGENVNNQNAEETRQALQ
KYTIDLTERAKAGKLDPVIGRDEEIRRAVQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKNKRVLSLDM
GALIAGAKYRGEFEERLKAVLNELAKEEGQVILFIDEIHTMVGAGKTDGAMDAGNLLKPSLARGELHCVGATTLDEYRQY
IEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYEIHHHVQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAASSLR
MEIDSKPEPLDKLERRIIQLKLERQALQKEEDEASRQRLAKLDEELTAREREYSELEEVWKAEKSALLGTQHIKTELENA
RIEMDQARRENNFEKMSELQYGKIPALEKQLHEAVKREEEGSENQLLRTKVTEEEIAEVLSKATGIPVSKMMEGEKEKLL
RMEEVLHSRVIGQNEAVEAVANAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKTLANFLFDDPDAMVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAARRRPYSVVLLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSHLIQENPELDYAGMKEVVMSVVGQHFRPEFINRIDETVVFHPLGQAHIRAIARIQLQRLIARLAERGYEVTVTDAALD
HIGKAGFDPLFGARPLKRAIQQELENPLSQQILSGKLLPNSPVVVDYQNDRLSISNS

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=412, Percent_Identity=33.0097087378641, Blast_Score=198, Evalue=2e-50,
Organism=Escherichia coli, GI1788943, Length=855, Percent_Identity=74.6198830409357, Blast_Score=1305, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=391, Percent_Identity=40.4092071611253, Blast_Score=277, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6320464, Length=709, Percent_Identity=52.1861777150917, Blast_Score=756, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=862, Percent_Identity=42.6914153132251, Blast_Score=667, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95774; Mature: 95774

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFEKFTTKLQEALAEAQSLAIGKDNNYIEPAHLLYALVKQPDGSIAPLFTALNVQPQRI
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCHHHH
LSELETILNRLPTVSGGTTQPSQQLIRLLNQCDKLAQQFGDSFISSELFVLAALDDNGDL
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCH
GKLLKQFGLTKEKVNAAISQIRGGENVNNQNAEETRQALQKYTIDLTERAKAGKLDPVIG
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
RDEEIRRAVQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKNKRVLSLDM
CCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECH
GALIAGAKYRGEFEERLKAVLNELAKEEGQVILFIDEIHTMVGAGKTDGAMDAGNLLKPS
HHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCC
LARGELHCVGATTLDEYRQYIEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYEIHHH
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
VQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAASSLRMEIDSKPEPLDKLERRIIQL
EEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHH
KLERQALQKEEDEASRQRLAKLDEELTAREREYSELEEVWKAEKSALLGTQHIKTELENA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIEMDQARRENNFEKMSELQYGKIPALEKQLHEAVKREEEGSENQLLRTKVTEEEIAEVL
HHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHH
SKATGIPVSKMMEGEKEKLLRMEEVLHSRVIGQNEAVEAVANAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKTLANFLFDDPDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAARRRPYSVVLLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
CHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCH
GSHLIQENPELDYAGMKEVVMSVVGQHFRPEFINRIDETVVFHPLGQAHIRAIARIQLQR
HHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHHH
LIARLAERGYEVTVTDAALDHIGKAGFDPLFGARPLKRAIQQELENPLSQQILSGKLLPN
HHHHHHHCCCEEEEEHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCC
SPVVVDYQNDRLSISNS
CCEEEEECCCEEECCCC
>Mature Secondary Structure
MNFEKFTTKLQEALAEAQSLAIGKDNNYIEPAHLLYALVKQPDGSIAPLFTALNVQPQRI
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCHHHH
LSELETILNRLPTVSGGTTQPSQQLIRLLNQCDKLAQQFGDSFISSELFVLAALDDNGDL
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCH
GKLLKQFGLTKEKVNAAISQIRGGENVNNQNAEETRQALQKYTIDLTERAKAGKLDPVIG
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
RDEEIRRAVQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKNKRVLSLDM
CCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECH
GALIAGAKYRGEFEERLKAVLNELAKEEGQVILFIDEIHTMVGAGKTDGAMDAGNLLKPS
HHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCC
LARGELHCVGATTLDEYRQYIEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYEIHHH
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
VQITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAASSLRMEIDSKPEPLDKLERRIIQL
EEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHH
KLERQALQKEEDEASRQRLAKLDEELTAREREYSELEEVWKAEKSALLGTQHIKTELENA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIEMDQARRENNFEKMSELQYGKIPALEKQLHEAVKREEEGSENQLLRTKVTEEEIAEVL
HHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHH
SKATGIPVSKMMEGEKEKLLRMEEVLHSRVIGQNEAVEAVANAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKTLANFLFDDPDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAARRRPYSVVLLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
CHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCH
GSHLIQENPELDYAGMKEVVMSVVGQHFRPEFINRIDETVVFHPLGQAHIRAIARIQLQR
HHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHHH
LIARLAERGYEVTVTDAALDHIGKAGFDPLFGARPLKRAIQQELENPLSQQILSGKLLPN
HHHHHHHCCCEEEEEHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCC
SPVVVDYQNDRLSISNS
CCEEEEECCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA