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The map label for this gene is thiI [H]

Identifier: 126208546

GI number: 126208546

Start: 1241813

End: 1243261

Strand: Reverse

Name: thiI [H]

Synonym: APL_1074

Alternate gene names: 126208546

Gene position: 1243261-1241813 (Counterclockwise)

Preceding gene: 126208547

Following gene: 126208544

Centisome position: 54.66

GC content: 41.48

Gene sequence:

>1449_bases
ATGAAATTTATTATTAAACTTTTCCCTGAAATTATGATTAAAAGCGATTCGGTTCGTAAACGCTTTATCAAAATTTTGAC
TTCCAATATCCGCAATTTGTTATTGCGTGAAACCGAAAATGTCGCGGTTATTCGTAACTGGGACTTTATTGAAGTGCGTG
CCAAAGTGGCGGAAGAAGCGCCGTTGGTATTGGAATTACTGAAACGTACGCCGGGTATTCACCACATTTTAGAAGTGGAA
GAAATGCCGTTTACTACGATGCACGATATCTTTGAAAATACATTGGCAAAAGTACGTGATGAGTTAGAAGGTAAAACGTT
CTGCGTGCGTGTACGCCGTAAAGGTAAGCACGAATTCCGTTCGTTAGATGTAGAAAAATATGTGGGTGGCGGTTTAAATC
AACATATTGAATCGGCTCGGGTGAAATTAACCAAGCCGGATGTAACCGTTCGTATTGATATTGACGGCGATAAAATGTTG
CTGATCAATGCTCGTCACGAAGGTTTAGGCGGTTATCCGATCGGTACGCAAGAAGATGTATTATCGCTTATTTCGGGCGG
ATTCGATTCGGGTGTATCAAGCTATATGCTGATTCGCCGTGGTTCACGTGTACATTACTGCTTCTTTAATTTAGGCGGTG
CGGCGCACGAAATCGGTGTAAAACAAATGGCATACCATATTTGGAGCCGTTACAGCACTTCACACAAAGTCCGTTTTGTG
GCGATTAATTTTGAAAATGTGGTCGGCGAAATTTTAGAGAAAGTTGATAATGGTCAAATGGGCGTGGTGTTAAAACGTAT
GATGGTGCGTGCCGCGAGCAAAATTGCCGAGCGTTTTGATATTCAAGCGATCGTAACCGGCGAAGCGTTAGGTCAGGTTT
CAAGCCAAACCTTAACCAATTTACGTTTAATCGACAAAGCGTCCGATACATTGGTATTACGTCCGTTAATCACGCACGAT
AAAGAGCAAATTATTGCCATGGCAAAAGAAATCGGCACCGATGATATTGCTAAATCCATGCCGGAATTTTGTGGCGTTAT
CTCGAAAAATCCGACGGTGAAAGCGATTGAAAGCAAAATTGTAGAAGAGGAAGGACATTTCAATTTTGAAGTGTTGGAAC
AAGCAGTGGCAAACGCACAGTTCCTCGATATTCGTGAGATTGCTCAACAAACGGAACAAGAGGTCGTGTCGGTTGAAACC
ACTTCCGAATTAACCGAAAACGATATTATTTTGGATATTCGTAGTCCGGAAGAAACTGATGAGGCTCCGTTTAGTTTGGA
CGGTATAGAAGTAAAATTAATGCCGTTCTATAAACTTTCAAGCCAGTTCGCTTCGTTGGATCAATCGAAAAACTACTTAC
TTTACTGCCAACGTGGGGTAATGAGCAAATTACAAGCGCTTTATCTCAAAGAGAATGGCTTTAGTAATGTAAAAGTGTTT
CGACACTAA

Upstream 100 bases:

>100_bases
TTGTTTTTTGATACTTGTCTGAAAAGCAGTTAAATTTGCAAATTTTTAGCGAAATTTAACCGCTTGTTTGGGCAACTTTT
TTCTGTAGTGAAACCGAAAA

Downstream 100 bases:

>100_bases
TGATTTTATAGATGAAAAGCGGTCATTTTGGGTTAAACTTTTGCAAGTTTTTGCCGAAAAATGGCCGCTTTCTATTAAGC
TATTACACTAAATCATTAAG

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKFIIKLFPEIMIKSDSVRKRFIKILTSNIRNLLLRETENVAVIRNWDFIEVRAKVAEEAPLVLELLKRTPGIHHILEVE
EMPFTTMHDIFENTLAKVRDELEGKTFCVRVRRKGKHEFRSLDVEKYVGGGLNQHIESARVKLTKPDVTVRIDIDGDKML
LINARHEGLGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWSRYSTSHKVRFV
AINFENVVGEILEKVDNGQMGVVLKRMMVRAASKIAERFDIQAIVTGEALGQVSSQTLTNLRLIDKASDTLVLRPLITHD
KEQIIAMAKEIGTDDIAKSMPEFCGVISKNPTVKAIESKIVEEEGHFNFEVLEQAVANAQFLDIREIAQQTEQEVVSVET
TSELTENDIILDIRSPEETDEAPFSLDGIEVKLMPFYKLSSQFASLDQSKNYLLYCQRGVMSKLQALYLKENGFSNVKVF
RH

Sequences:

>Translated_482_residues
MKFIIKLFPEIMIKSDSVRKRFIKILTSNIRNLLLRETENVAVIRNWDFIEVRAKVAEEAPLVLELLKRTPGIHHILEVE
EMPFTTMHDIFENTLAKVRDELEGKTFCVRVRRKGKHEFRSLDVEKYVGGGLNQHIESARVKLTKPDVTVRIDIDGDKML
LINARHEGLGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWSRYSTSHKVRFV
AINFENVVGEILEKVDNGQMGVVLKRMMVRAASKIAERFDIQAIVTGEALGQVSSQTLTNLRLIDKASDTLVLRPLITHD
KEQIIAMAKEIGTDDIAKSMPEFCGVISKNPTVKAIESKIVEEEGHFNFEVLEQAVANAQFLDIREIAQQTEQEVVSVET
TSELTENDIILDIRSPEETDEAPFSLDGIEVKLMPFYKLSSQFASLDQSKNYLLYCQRGVMSKLQALYLKENGFSNVKVF
RH
>Mature_482_residues
MKFIIKLFPEIMIKSDSVRKRFIKILTSNIRNLLLRETENVAVIRNWDFIEVRAKVAEEAPLVLELLKRTPGIHHILEVE
EMPFTTMHDIFENTLAKVRDELEGKTFCVRVRRKGKHEFRSLDVEKYVGGGLNQHIESARVKLTKPDVTVRIDIDGDKML
LINARHEGLGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWSRYSTSHKVRFV
AINFENVVGEILEKVDNGQMGVVLKRMMVRAASKIAERFDIQAIVTGEALGQVSSQTLTNLRLIDKASDTLVLRPLITHD
KEQIIAMAKEIGTDDIAKSMPEFCGVISKNPTVKAIESKIVEEEGHFNFEVLEQAVANAQFLDIREIAQQTEQEVVSVET
TSELTENDIILDIRSPEETDEAPFSLDGIEVKLMPFYKLSSQFASLDQSKNYLLYCQRGVMSKLQALYLKENGFSNVKVF
RH

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=481, Percent_Identity=69.6465696465696, Blast_Score=694, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 54606; Mature: 54606

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS51165 THUMP ; PS01165 COPPER_AMINE_OXID_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIIKLFPEIMIKSDSVRKRFIKILTSNIRNLLLRETENVAVIRNWDFIEVRAKVAEEA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEHHHHHHCCC
PLVLELLKRTPGIHHILEVEEMPFTTMHDIFENTLAKVRDELEGKTFCVRVRRKGKHEFR
HHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCHHHH
SLDVEKYVGGGLNQHIESARVKLTKPDVTVRIDIDGDKMLLINARHEGLGGYPIGTQEDV
HCCHHHHHCCCHHHHHHHCEEEEECCCEEEEEEECCCEEEEEEECCCCCCCCCCCCHHHH
LSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWSRYSTSHKVRFV
HHHHHCCCCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
AINFENVVGEILEKVDNGQMGVVLKRMMVRAASKIAERFDIQAIVTGEALGQVSSQTLTN
EEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHH
LRLIDKASDTLVLRPLITHDKEQIIAMAKEIGTDDIAKSMPEFCGVISKNPTVKAIESKI
HEEEECCCCCEEEEHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
VEEEGHFNFEVLEQAVANAQFLDIREIAQQTEQEVVSVETTSELTENDIILDIRSPEETD
HHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEHHHHCCCCCEEEEECCCCCCC
EAPFSLDGIEVKLMPFYKLSSQFASLDQSKNYLLYCQRGVMSKLQALYLKENGFSNVKVF
CCCCEECCEEEEEEEHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCCEEEE
RH
EC
>Mature Secondary Structure
MKFIIKLFPEIMIKSDSVRKRFIKILTSNIRNLLLRETENVAVIRNWDFIEVRAKVAEEA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEHHHHHHCCC
PLVLELLKRTPGIHHILEVEEMPFTTMHDIFENTLAKVRDELEGKTFCVRVRRKGKHEFR
HHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCHHHH
SLDVEKYVGGGLNQHIESARVKLTKPDVTVRIDIDGDKMLLINARHEGLGGYPIGTQEDV
HCCHHHHHCCCHHHHHHHCEEEEECCCEEEEEEECCCEEEEEEECCCCCCCCCCCCHHHH
LSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEIGVKQMAYHIWSRYSTSHKVRFV
HHHHHCCCCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
AINFENVVGEILEKVDNGQMGVVLKRMMVRAASKIAERFDIQAIVTGEALGQVSSQTLTN
EEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHH
LRLIDKASDTLVLRPLITHDKEQIIAMAKEIGTDDIAKSMPEFCGVISKNPTVKAIESKI
HEEEECCCCCEEEEHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
VEEEGHFNFEVLEQAVANAQFLDIREIAQQTEQEVVSVETTSELTENDIILDIRSPEETD
HHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEHHHHCCCCCEEEEECCCCCCC
EAPFSLDGIEVKLMPFYKLSSQFASLDQSKNYLLYCQRGVMSKLQALYLKENGFSNVKVF
CCCCEECCEEEEEEEHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCCEEEE
RH
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA