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The map label for this gene is purA

Identifier: 126208547

GI number: 126208547

Start: 1243448

End: 1244746

Strand: Reverse

Name: purA

Synonym: APL_1075

Alternate gene names: 126208547

Gene position: 1244746-1243448 (Counterclockwise)

Preceding gene: 126208548

Following gene: 126208546

Centisome position: 54.73

GC content: 44.96

Gene sequence:

>1299_bases
ATGGGTAAAAGTGTTGCTATTCTCGGCGCTCAGTGGGGCGATGAAGGGAAAGGTAAAATCGTAGATTTATTAACGGATCG
TGTGAAATACGTAGTTCGTTATCAAGGGGGTCACAATGCGGGCCATACCTTAATTATCAATGGCGAAAAAACCGTTCTTC
GTCTGATTCCGAGCGGTATTTTACGTGATAATGTAACCTGTTTAATCGGTAACGGTGTGGTGCTTTCTCCGGAAGCGTTA
ATGAAAGAAATGGGTGAATTGGAAGCCCGTGGCATTAACGTTCGTGATCGTTTAAAAATTTCAGAGGCTTGCCCGTTAAT
TCTTCCTTACCATGTGGCAATGGATCACGCGCGTGAAGCGGCGTTAGGTAAAAACAAAATCGGTACGACCGGTCGCGGTA
TCGGTCCGGCGTACGAGGATAAAGTGGCTCGCCGCGGTTTACGTGTCAGCGATTTATTTGATAAAGAAGCCTTTGCTGAA
AAATTAAAAGATATTTTGGATTACTACAATTTCCAATTAGTACATTACTACAAAGTTGAACCGGTAGATTTCCAAAAAAC
ATTAGATGACGTATTTGCGATTGCCGATGTAATTAAAGGTATGGTGGCGGACGTAACAACCTTACTTCACCAAGCACGTA
AAGAAGGCGTGAATATCTTATTCGAAGGTGCGCAAGGCACAATGTTAGATATCGACCACGGTACATATCCGTTTGTAACC
AGCTCAAATACTACGGCGGGTGGTGTTGCAACCGGTTCGGGTTTCGGTCCTCGTAATCTTGACTATGTATTGGGTATTAT
CAAAGCATACTGTACTCGTGTCGGTAGCGGTCCGTTCACCACTGAATTATTTGATGAAGTGGGGGCGGAAATCGCTCGTA
AAGGTAACGAGTTCGGTGCGGTAACCGGTCGTCCGCGCCGTTGCGGTTGGTTTGATGCGGTAGCTGTACGCCGTGCAGTA
CAAATTAACTCGATTTCAGGCTTCTGTATGACAAAATTAGACGTATTAGACGGCTTTGAAGAGTTAAAAATTTGTACTGC
ATACAAAATGCCAAACGGTGAAATCGTAGAATACGCACCAATGGCGGCGAAAGATTGGAAAGGGGTCGAGCCGATTTACG
AAACCATGCCGGGCTGGTCTGAAAATACCTTCCGTGTAACTAAACGTGAAGAATTACCGCAAGCGGCGTTAGATTACATC
AAACGTATTGAAGAGCTTGTCGGTGTACCGGTTGATATTCTTTCAACAGGTCCGGATCGTGTAGAAACAATGATCTTACG
TGACCCGTTTGCGGCGTAA

Upstream 100 bases:

>100_bases
CTGTTTTTTAGCAAATTTTTTATCGAATCCGACCGCTTGTCGTGTGTTTGGAGCGAGTACACCAATTTTTCATTTGAAAC
TAACTAAAGAGGCAATTCCT

Downstream 100 bases:

>100_bases
TTTCGTTTAAATTTTAAGTTGTTTTCTTACCACGGAAAACACGGAGTTCACGGAATTTTCTGTGTATTCCGTGCTTTCCG
TGGTAATTGTTTTTTGATAC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MGKSVAILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTLIINGEKTVLRLIPSGILRDNVTCLIGNGVVLSPEAL
MKEMGELEARGINVRDRLKISEACPLILPYHVAMDHAREAALGKNKIGTTGRGIGPAYEDKVARRGLRVSDLFDKEAFAE
KLKDILDYYNFQLVHYYKVEPVDFQKTLDDVFAIADVIKGMVADVTTLLHQARKEGVNILFEGAQGTMLDIDHGTYPFVT
SSNTTAGGVATGSGFGPRNLDYVLGIIKAYCTRVGSGPFTTELFDEVGAEIARKGNEFGAVTGRPRRCGWFDAVAVRRAV
QINSISGFCMTKLDVLDGFEELKICTAYKMPNGEIVEYAPMAAKDWKGVEPIYETMPGWSENTFRVTKREELPQAALDYI
KRIEELVGVPVDILSTGPDRVETMILRDPFAA

Sequences:

>Translated_432_residues
MGKSVAILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTLIINGEKTVLRLIPSGILRDNVTCLIGNGVVLSPEAL
MKEMGELEARGINVRDRLKISEACPLILPYHVAMDHAREAALGKNKIGTTGRGIGPAYEDKVARRGLRVSDLFDKEAFAE
KLKDILDYYNFQLVHYYKVEPVDFQKTLDDVFAIADVIKGMVADVTTLLHQARKEGVNILFEGAQGTMLDIDHGTYPFVT
SSNTTAGGVATGSGFGPRNLDYVLGIIKAYCTRVGSGPFTTELFDEVGAEIARKGNEFGAVTGRPRRCGWFDAVAVRRAV
QINSISGFCMTKLDVLDGFEELKICTAYKMPNGEIVEYAPMAAKDWKGVEPIYETMPGWSENTFRVTKREELPQAALDYI
KRIEELVGVPVDILSTGPDRVETMILRDPFAA
>Mature_431_residues
GKSVAILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTLIINGEKTVLRLIPSGILRDNVTCLIGNGVVLSPEALM
KEMGELEARGINVRDRLKISEACPLILPYHVAMDHAREAALGKNKIGTTGRGIGPAYEDKVARRGLRVSDLFDKEAFAEK
LKDILDYYNFQLVHYYKVEPVDFQKTLDDVFAIADVIKGMVADVTTLLHQARKEGVNILFEGAQGTMLDIDHGTYPFVTS
SNTTAGGVATGSGFGPRNLDYVLGIIKAYCTRVGSGPFTTELFDEVGAEIARKGNEFGAVTGRPRRCGWFDAVAVRRAVQ
INSISGFCMTKLDVLDGFEELKICTAYKMPNGEIVEYAPMAAKDWKGVEPIYETMPGWSENTFRVTKREELPQAALDYIK
RIEELVGVPVDILSTGPDRVETMILRDPFAA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=42.018779342723, Blast_Score=319, Evalue=3e-87,
Organism=Homo sapiens, GI34577063, Length=432, Percent_Identity=42.1296296296296, Blast_Score=318, Evalue=9e-87,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=40.4522613065327, Blast_Score=282, Evalue=5e-76,
Organism=Escherichia coli, GI1790620, Length=432, Percent_Identity=75.462962962963, Blast_Score=688, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146553, Length=434, Percent_Identity=42.1658986175115, Blast_Score=333, Evalue=8e-92,
Organism=Caenorhabditis elegans, GI25146555, Length=434, Percent_Identity=42.1658986175115, Blast_Score=333, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6324109, Length=439, Percent_Identity=42.369020501139, Blast_Score=337, Evalue=3e-93,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=41.8052256532066, Blast_Score=332, Evalue=2e-91,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_ACTP2 (A3N181)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053772.1
- ProteinModelPortal:   A3N181
- SMR:   A3N181
- STRING:   A3N181
- GeneID:   4850489
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1075
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- PhylomeDB:   A3N181
- ProtClustDB:   PRK01117
- BioCyc:   APLE416269:APL_1075-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47457; Mature: 47326

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 14-14 ACT_SITE 42-42 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSVAILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTLIINGEKTVLRLIPSGI
CCCCEEEEECCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCHHEEHHHCCHH
LRDNVTCLIGNGVVLSPEALMKEMGELEARGINVRDRLKISEACPLILPYHVAMDHAREA
HHCCCEEEECCCEEECHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHH
ALGKNKIGTTGRGIGPAYEDKVARRGLRVSDLFDKEAFAEKLKDILDYYNFQLVHYYKVE
HCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEC
PVDFQKTLDDVFAIADVIKGMVADVTTLLHQARKEGVNILFEGAQGTMLDIDHGTYPFVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEEE
SSNTTAGGVATGSGFGPRNLDYVLGIIKAYCTRVGSGPFTTELFDEVGAEIARKGNEFGA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
VTGRPRRCGWFDAVAVRRAVQINSISGFCMTKLDVLDGFEELKICTAYKMPNGEIVEYAP
CCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCHHHHEEEEEEECCCCCEEEECC
MAAKDWKGVEPIYETMPGWSENTFRVTKREELPQAALDYIKRIEELVGVPVDILSTGPDR
CCCCCCCCCCHHHHHCCCCCCCCEEECCHHHCCHHHHHHHHHHHHHHCCCHHHHCCCHHH
VETMILRDPFAA
HHEEEECCCCCC
>Mature Secondary Structure 
GKSVAILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTLIINGEKTVLRLIPSGI
CCCEEEEECCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCHHEEHHHCCHH
LRDNVTCLIGNGVVLSPEALMKEMGELEARGINVRDRLKISEACPLILPYHVAMDHAREA
HHCCCEEEECCCEEECHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHH
ALGKNKIGTTGRGIGPAYEDKVARRGLRVSDLFDKEAFAEKLKDILDYYNFQLVHYYKVE
HCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEC
PVDFQKTLDDVFAIADVIKGMVADVTTLLHQARKEGVNILFEGAQGTMLDIDHGTYPFVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEEE
SSNTTAGGVATGSGFGPRNLDYVLGIIKAYCTRVGSGPFTTELFDEVGAEIARKGNEFGA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCC
VTGRPRRCGWFDAVAVRRAVQINSISGFCMTKLDVLDGFEELKICTAYKMPNGEIVEYAP
CCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCHHHHEEEEEEECCCCCEEEECC
MAAKDWKGVEPIYETMPGWSENTFRVTKREELPQAALDYIKRIEELVGVPVDILSTGPDR
CCCCCCCCCCHHHHHCCCCCCCCEEECCHHHCCHHHHHHHHHHHHHHCCCHHHHCCCHHH
VETMILRDPFAA
HHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA