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The map label for this gene is kefBC [H]

Identifier: 126208525

GI number: 126208525

Start: 1222558

End: 1224423

Strand: Reverse

Name: kefBC [H]

Synonym: APL_1053

Alternate gene names: 126208525

Gene position: 1224423-1222558 (Counterclockwise)

Preceding gene: 126208526

Following gene: 126208524

Centisome position: 53.83

GC content: 46.2

Gene sequence:

>1866_bases
ATGGCGATAGATATCTCTCATTTGGATAATCCGGAATTAGCAAAAATCGTAGCGTTACTCGCTACCAGTATTACGATTGT
GCCGTTATTTAAACGTATCGGGCTGGGTAGCGTACTGGGTTATTTAGTGGCGGGATGTCTGATCGGTCCGTCCGTGTTGG
GGCTGATTCAAGATCCGCAAGCGGTCGTCCATTTGGCGGAACTCGGTGTCGTGATGTTCCTATTCATTATCGGTTTGGAA
ATGCACCCGGAGCTATTGTGGAGTATGCGTAAAGCTATTTTCGGAAGAGGCTTCTTACAAGTGGCGACTTGCGGCGTTTT
ATTGACTTTAGCCGGTATCTATATTCTGGGTTTAACTAAAGAAGTGGCGTTTATCGCCGGTGCGGGTTTTACCCTGTCTT
CCACCGCGATTGTGATGCAAGTGCTTGAAGATAAAGGGATTGCCAGCACGCAAAAAGGACAGCGCATCGTTTCGACTTTA
TTATTTGAAGATTTGGCTATCGTGCCTTTGTTGGCGACGATTGCCTTTTTGGCGCCGGAACAAGCGAATGAAGAAAAAAC
ATTCAGCTTAACTTCTATCGGTATTGCGATTGTCGGCATTATTATTTTAGTTGTCGGCAGTAAATGGGTAATGAATCCGC
TATTTAGAATGATCTCGAAAGCGAAAGTACGTGAAATGATGACGGCCGCAGCGTTATTAGTGGTTCTCGGTGCGGCGTTA
TTGATGGAAATGAGCGGGCTTTCGATGGCAATGGGAGCGTTCGTTGCCGGAGTTGTTCTGTCCGAATCTTCTTTCCGTCA
CCAATTGGAAGCGGATATCGAGCCGTTCAGGGGCTTATTGCTCGGGCTGTTTTTTATGGGCGTCGGGATGTCGCTTGATC
TGAATTTCGTATTTAACGATTTAATTTGGTTATTAAGTATCGTAGCGGTCTGTGTGTTAGGGAAAGCGTTCGGCGTTTAT
ATCATCGCTTTAATTACACGATTGCCTCATCGCGAAGCCTTGATGCGTACTTCGATTATGAGCCATGGCGGCGAATTTGC
ATTCGTCCTGTTTTCCGCAGCGGCGGCGGCAGGCTTGTTGGATCAAGATCAACAGGCGACTTTTACCGCAGCGGTGATTG
TGTCGATGTTAATCTCGCCGTTAGTGATGTTGCTGATGCAGCGTATGGTTGCGCGTAAAGCGAGTGTCGAAGCGCCGAAT
ATGATGGGGATTGAGATTGCCGAAGATTTGGAGAACAGCGTAATTGTTATCGGATTCGGGCGTTTTAGTCAGATTGTCTG
TCAGGCATTGTTAGCGAGAGGTATTACGGTTACCGTGATTGATTCAAGTACCGATCGTATTCGAGCAGCGGCTACTTTCG
GGTTTAAAGTGTATTATGGGGACGGTACTCGACTTGACGTGTTGCGAGCAAGCGGATTGGAAAAAGCCGATTGTGTCGTA
TTGGGCATCAATAATCCGGCGCGTAGCGCATTAATCGTAGAACAAATCAAAAACGAATATCCGCTCACGCCGATCTTTGC
ACGAACTTATGACCGCCATAGTGCGATTGAATTAACGAAATTACATGTAGATTTCCAAATTCGTGAAACCTTGGAATCCG
CATTGGTATTGAGTAAAGCGGCAATGATGAAATTAGGGGTGGACGAAGTGGAAGCAACCGAAATTGTGGAGAATGTACGT
TTGCTGGATAGAGAGCGTTTTAAAGAAGAACTGATTTACGGCACTTCGGCGGAATTGATTCGTAAATATTTTACGCCGAA
GCCGTTTTTTAAGCCGCAACAAGAAGCGGAAGCCTTAAATGAAGATGCGGCGGAAATCTTAGCGGAAGAAGCGGTCGAGT
CAAATGCGGAAAGCAAAGAGAAATAA

Upstream 100 bases:

>100_bases
CAACAAGGATAATATGCTTATTACTCAACTGCTAAATTGATGTGCGTATCGAAGTAAACAAGCATGGATTTAGTTATTTT
ATATTTGGAACCTCAATTTT

Downstream 100 bases:

>100_bases
ACGTCTGAAAAATTCGTATTTGATCAAATATCTTAACAACAAAGGGCGAGTTATCGCCCTTTTCGTTTACATAAAGGTAA
CAAATCTTTATGATTAGCCT

Product: glutathione-regulated potassium-efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MAIDISHLDNPELAKIVALLATSITIVPLFKRIGLGSVLGYLVAGCLIGPSVLGLIQDPQAVVHLAELGVVMFLFIIGLE
MHPELLWSMRKAIFGRGFLQVATCGVLLTLAGIYILGLTKEVAFIAGAGFTLSSTAIVMQVLEDKGIASTQKGQRIVSTL
LFEDLAIVPLLATIAFLAPEQANEEKTFSLTSIGIAIVGIIILVVGSKWVMNPLFRMISKAKVREMMTAAALLVVLGAAL
LMEMSGLSMAMGAFVAGVVLSESSFRHQLEADIEPFRGLLLGLFFMGVGMSLDLNFVFNDLIWLLSIVAVCVLGKAFGVY
IIALITRLPHREALMRTSIMSHGGEFAFVLFSAAAAAGLLDQDQQATFTAAVIVSMLISPLVMLLMQRMVARKASVEAPN
MMGIEIAEDLENSVIVIGFGRFSQIVCQALLARGITVTVIDSSTDRIRAAATFGFKVYYGDGTRLDVLRASGLEKADCVV
LGINNPARSALIVEQIKNEYPLTPIFARTYDRHSAIELTKLHVDFQIRETLESALVLSKAAMMKLGVDEVEATEIVENVR
LLDRERFKEELIYGTSAELIRKYFTPKPFFKPQQEAEALNEDAAEILAEEAVESNAESKEK

Sequences:

>Translated_621_residues
MAIDISHLDNPELAKIVALLATSITIVPLFKRIGLGSVLGYLVAGCLIGPSVLGLIQDPQAVVHLAELGVVMFLFIIGLE
MHPELLWSMRKAIFGRGFLQVATCGVLLTLAGIYILGLTKEVAFIAGAGFTLSSTAIVMQVLEDKGIASTQKGQRIVSTL
LFEDLAIVPLLATIAFLAPEQANEEKTFSLTSIGIAIVGIIILVVGSKWVMNPLFRMISKAKVREMMTAAALLVVLGAAL
LMEMSGLSMAMGAFVAGVVLSESSFRHQLEADIEPFRGLLLGLFFMGVGMSLDLNFVFNDLIWLLSIVAVCVLGKAFGVY
IIALITRLPHREALMRTSIMSHGGEFAFVLFSAAAAAGLLDQDQQATFTAAVIVSMLISPLVMLLMQRMVARKASVEAPN
MMGIEIAEDLENSVIVIGFGRFSQIVCQALLARGITVTVIDSSTDRIRAAATFGFKVYYGDGTRLDVLRASGLEKADCVV
LGINNPARSALIVEQIKNEYPLTPIFARTYDRHSAIELTKLHVDFQIRETLESALVLSKAAMMKLGVDEVEATEIVENVR
LLDRERFKEELIYGTSAELIRKYFTPKPFFKPQQEAEALNEDAAEILAEEAVESNAESKEK
>Mature_620_residues
AIDISHLDNPELAKIVALLATSITIVPLFKRIGLGSVLGYLVAGCLIGPSVLGLIQDPQAVVHLAELGVVMFLFIIGLEM
HPELLWSMRKAIFGRGFLQVATCGVLLTLAGIYILGLTKEVAFIAGAGFTLSSTAIVMQVLEDKGIASTQKGQRIVSTLL
FEDLAIVPLLATIAFLAPEQANEEKTFSLTSIGIAIVGIIILVVGSKWVMNPLFRMISKAKVREMMTAAALLVVLGAALL
MEMSGLSMAMGAFVAGVVLSESSFRHQLEADIEPFRGLLLGLFFMGVGMSLDLNFVFNDLIWLLSIVAVCVLGKAFGVYI
IALITRLPHREALMRTSIMSHGGEFAFVLFSAAAAAGLLDQDQQATFTAAVIVSMLISPLVMLLMQRMVARKASVEAPNM
MGIEIAEDLENSVIVIGFGRFSQIVCQALLARGITVTVIDSSTDRIRAAATFGFKVYYGDGTRLDVLRASGLEKADCVVL
GINNPARSALIVEQIKNEYPLTPIFARTYDRHSAIELTKLHVDFQIRETLESALVLSKAAMMKLGVDEVEATEIVENVRL
LDRERFKEELIYGTSAELIRKYFTPKPFFKPQQEAEALNEDAAEILAEEAVESNAESKEK

Specific function: Transport system that facilitate potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=386, Percent_Identity=27.4611398963731, Blast_Score=77, Evalue=6e-14,
Organism=Escherichia coli, GI1786232, Length=563, Percent_Identity=38.898756660746, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1789749, Length=553, Percent_Identity=38.6980108499096, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1786685, Length=523, Percent_Identity=27.3422562141491, Blast_Score=168, Evalue=8e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 67365; Mature: 67233

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIDISHLDNPELAKIVALLATSITIVPLFKRIGLGSVLGYLVAGCLIGPSVLGLIQDPQ
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCHH
AVVHLAELGVVMFLFIIGLEMHPELLWSMRKAIFGRGFLQVATCGVLLTLAGIYILGLTK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
EVAFIAGAGFTLSSTAIVMQVLEDKGIASTQKGQRIVSTLLFEDLAIVPLLATIAFLAPE
HHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QANEEKTFSLTSIGIAIVGIIILVVGSKWVMNPLFRMISKAKVREMMTAAALLVVLGAAL
CCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMEMSGLSMAMGAFVAGVVLSESSFRHQLEADIEPFRGLLLGLFFMGVGMSLDLNFVFND
HHHHHCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEHHHHHHH
LIWLLSIVAVCVLGKAFGVYIIALITRLPHREALMRTSIMSHGGEFAFVLFSAAAAAGLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
DQDQQATFTAAVIVSMLISPLVMLLMQRMVARKASVEAPNMMGIEIAEDLENSVIVIGFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEECC
RFSQIVCQALLARGITVTVIDSSTDRIRAAATFGFKVYYGDGTRLDVLRASGLEKADCVV
HHHHHHHHHHHHCCCEEEEECCCCCHHHHEEEEEEEEEECCCCEEEHHHHCCCCCCCEEE
LGINNPARSALIVEQIKNEYPLTPIFARTYDRHSAIELTKLHVDFQIRETLESALVLSKA
EECCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHH
AMMKLGVDEVEATEIVENVRLLDRERFKEELIYGTSAELIRKYFTPKPFFKPQQEAEALN
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHH
EDAAEILAEEAVESNAESKEK
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AIDISHLDNPELAKIVALLATSITIVPLFKRIGLGSVLGYLVAGCLIGPSVLGLIQDPQ
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCHH
AVVHLAELGVVMFLFIIGLEMHPELLWSMRKAIFGRGFLQVATCGVLLTLAGIYILGLTK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
EVAFIAGAGFTLSSTAIVMQVLEDKGIASTQKGQRIVSTLLFEDLAIVPLLATIAFLAPE
HHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QANEEKTFSLTSIGIAIVGIIILVVGSKWVMNPLFRMISKAKVREMMTAAALLVVLGAAL
CCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMEMSGLSMAMGAFVAGVVLSESSFRHQLEADIEPFRGLLLGLFFMGVGMSLDLNFVFND
HHHHHCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEHHHHHHH
LIWLLSIVAVCVLGKAFGVYIIALITRLPHREALMRTSIMSHGGEFAFVLFSAAAAAGLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
DQDQQATFTAAVIVSMLISPLVMLLMQRMVARKASVEAPNMMGIEIAEDLENSVIVIGFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEECC
RFSQIVCQALLARGITVTVIDSSTDRIRAAATFGFKVYYGDGTRLDVLRASGLEKADCVV
HHHHHHHHHHHHCCCEEEEECCCCCHHHHEEEEEEEEEECCCCEEEHHHHCCCCCCCEEE
LGINNPARSALIVEQIKNEYPLTPIFARTYDRHSAIELTKLHVDFQIRETLESALVLSKA
EECCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHH
AMMKLGVDEVEATEIVENVRLLDRERFKEELIYGTSAELIRKYFTPKPFFKPQQEAEALN
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHH
EDAAEILAEEAVESNAESKEK
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]