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The map label for this gene is menF [H]

Identifier: 126208516

GI number: 126208516

Start: 1209987

End: 1211267

Strand: Reverse

Name: menF [H]

Synonym: APL_1042

Alternate gene names: 126208516

Gene position: 1211267-1209987 (Counterclockwise)

Preceding gene: 126208521

Following gene: 126208514

Centisome position: 53.25

GC content: 42.23

Gene sequence:

>1281_bases
ATGTCTATTTTTAATCAATTAAAACAACAACTTAGCAAGCAATTAATAGAATCTGGAACTCATTTCGGTTTGATGGAGTA
TCAAGCCGAGGTTGCTTTGTCTGAAGAAAATATTGCGTTATTAAGTTGGTTAAAGGCACAAAATCATTATCCGCATTTTT
TTTGGCAGGCGAGAGAGACTGATCAGACCATTGCAAGTATCGGTGCAGTTCGCTCGTTTTTCAGTGTTGATGAAGCGCAG
CAATTTGTAAAACAAACGCAATTCGGCTTAGTCGGCGGCGTACGGTTTGAGGGCGGTTGTCAATTCATTCTGCCTCGTTT
GCTATTGGTAAAAAATCAGCAAAATTTAACCGCTTATTTCTACTTGAATGAAGCTGAATTAGCCCAACAAGCGGTCATTT
TTGAACGATTTTTTGCAAATTTCTGCCAAACCCTACCGCTTGAATTAACGGAAAATAATCTGCTTGCTCAGACTTCCGTT
TACGATTTTGATGGCTGGCAACAAAATATTGAGCGAGCGATTAGTCATATTCAGCAAAAAGACTTTAATAAAGTGGTACT
GGCTAACGCCAAAACGTTACAATTTGAAAATGAGCTATCCGCTTATGATTTACTTGCCGCCAGCCAAACCACTAATTTAG
GTTGTTACCATTTTATTTGGGCGGAAAATGTCGAATCGGCATTTGTCGGCTCAAGCCCGGAACGTTTATATCACCGAAAA
GGCAATCAATTTCATACGGAAGCATTAGCGGGTACCGCACCGGTAACGGAAAGTATGGTGCAGACGGAGCTAAATGCGCA
GTGGTTACTTACCGATCCGAAAAATATCTATGAAAATCAGTTGGTTGTAGATGATATTCAAGCACAATTAACCGATTGCG
TAACCGAATTGCAAATCAGCCAAGCGGAAATTAAACGTTTGCATAACGTGCAACATTTACGCCGTAAAATTTCCGCGGTA
TTAAAACCGGAGATGAGTGATGCGGATTGCCTTGCCCGTATGCATCCGACCGCAGCGGTTGCTGGCTTGCCTCGCCAAGC
TGCAAAACAATTTATTGCCGAATATGAGCAGTTCACTCGTTGTTGGTATGCCGGTACATTAGGCTATTTACAACCGGATG
AAGCGGAATTTTGCGTTGCTTTGCGTTCCGCGCAAATCGAACGAAACCGTATTACGCTCTATGCCGGTGCAGGTATTGTT
GAAGAGTCGGAACCGCAATCGGAATGGCAGGAAATTGAACGGAAGTCGTTGGCATTAGCCAAATTACTAAAAGTGGATTA
A

Upstream 100 bases:

>100_bases
CTCTGATTTAGTAGGAATTTAAAGAGGAAATGAGCTAAAATAATGACCTTTTTTCACAATAGCTAAAACTTAGCGGTCGA
ATTCTTCAGAAATTTTGCAA

Downstream 100 bases:

>100_bases
GAAACAAAAAATGGCAGGTATAACCCTGCCATTTTGTTTTATCCGGCTAAGCCGATAAATAATGCTAATAATAACGGTGC
AACCAAATTCACAATAAAGC

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSIFNQLKQQLSKQLIESGTHFGLMEYQAEVALSEENIALLSWLKAQNHYPHFFWQARETDQTIASIGAVRSFFSVDEAQ
QFVKQTQFGLVGGVRFEGGCQFILPRLLLVKNQQNLTAYFYLNEAELAQQAVIFERFFANFCQTLPLELTENNLLAQTSV
YDFDGWQQNIERAISHIQQKDFNKVVLANAKTLQFENELSAYDLLAASQTTNLGCYHFIWAENVESAFVGSSPERLYHRK
GNQFHTEALAGTAPVTESMVQTELNAQWLLTDPKNIYENQLVVDDIQAQLTDCVTELQISQAEIKRLHNVQHLRRKISAV
LKPEMSDADCLARMHPTAAVAGLPRQAAKQFIAEYEQFTRCWYAGTLGYLQPDEAEFCVALRSAQIERNRITLYAGAGIV
EESEPQSEWQEIERKSLALAKLLKVD

Sequences:

>Translated_426_residues
MSIFNQLKQQLSKQLIESGTHFGLMEYQAEVALSEENIALLSWLKAQNHYPHFFWQARETDQTIASIGAVRSFFSVDEAQ
QFVKQTQFGLVGGVRFEGGCQFILPRLLLVKNQQNLTAYFYLNEAELAQQAVIFERFFANFCQTLPLELTENNLLAQTSV
YDFDGWQQNIERAISHIQQKDFNKVVLANAKTLQFENELSAYDLLAASQTTNLGCYHFIWAENVESAFVGSSPERLYHRK
GNQFHTEALAGTAPVTESMVQTELNAQWLLTDPKNIYENQLVVDDIQAQLTDCVTELQISQAEIKRLHNVQHLRRKISAV
LKPEMSDADCLARMHPTAAVAGLPRQAAKQFIAEYEQFTRCWYAGTLGYLQPDEAEFCVALRSAQIERNRITLYAGAGIV
EESEPQSEWQEIERKSLALAKLLKVD
>Mature_425_residues
SIFNQLKQQLSKQLIESGTHFGLMEYQAEVALSEENIALLSWLKAQNHYPHFFWQARETDQTIASIGAVRSFFSVDEAQQ
FVKQTQFGLVGGVRFEGGCQFILPRLLLVKNQQNLTAYFYLNEAELAQQAVIFERFFANFCQTLPLELTENNLLAQTSVY
DFDGWQQNIERAISHIQQKDFNKVVLANAKTLQFENELSAYDLLAASQTTNLGCYHFIWAENVESAFVGSSPERLYHRKG
NQFHTEALAGTAPVTESMVQTELNAQWLLTDPKNIYENQLVVDDIQAQLTDCVTELQISQAEIKRLHNVQHLRRKISAVL
KPEMSDADCLARMHPTAAVAGLPRQAAKQFIAEYEQFTRCWYAGTLGYLQPDEAEFCVALRSAQIERNRITLYAGAGIVE
ESEPQSEWQEIERKSLALAKLLKVD

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=392, Percent_Identity=41.8367346938776, Blast_Score=278, Evalue=7e-76,
Organism=Escherichia coli, GI1786809, Length=275, Percent_Identity=27.6363636363636, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1787518, Length=273, Percent_Identity=27.8388278388278, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788114, Length=196, Percent_Identity=25.5102040816327, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=262, Percent_Identity=27.0992366412214, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 48461; Mature: 48330

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIFNQLKQQLSKQLIESGTHFGLMEYQAEVALSEENIALLSWLKAQNHYPHFFWQARET
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCCHHHHHHHHHCCCCCCEEEECCHH
DQTIASIGAVRSFFSVDEAQQFVKQTQFGLVGGVRFEGGCQFILPRLLLVKNQQNLTAYF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCEEEEE
YLNEAELAQQAVIFERFFANFCQTLPLELTENNLLAQTSVYDFDGWQQNIERAISHIQQK
EECHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
DFNKVVLANAKTLQFENELSAYDLLAASQTTNLGCYHFIWAENVESAFVGSSPERLYHRK
CCCEEEEECCCEEEECCCCCHHHHHHHCCCCCCCEEEEEECCCCCHHHCCCCHHHHHHHC
GNQFHTEALAGTAPVTESMVQTELNAQWLLTDPKNIYENQLVVDDIQAQLTDCVTELQIS
CCCEEHHHHCCCCHHHHHHHHHHCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHH
QAEIKRLHNVQHLRRKISAVLKPEMSDADCLARMHPTAAVAGLPRQAAKQFIAEYEQFTR
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHH
CWYAGTLGYLQPDEAEFCVALRSAQIERNRITLYAGAGIVEESEPQSEWQEIERKSLALA
HHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
KLLKVD
HHHHCC
>Mature Secondary Structure 
SIFNQLKQQLSKQLIESGTHFGLMEYQAEVALSEENIALLSWLKAQNHYPHFFWQARET
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCCHHHHHHHHHCCCCCCEEEECCHH
DQTIASIGAVRSFFSVDEAQQFVKQTQFGLVGGVRFEGGCQFILPRLLLVKNQQNLTAYF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCEEEEE
YLNEAELAQQAVIFERFFANFCQTLPLELTENNLLAQTSVYDFDGWQQNIERAISHIQQK
EECHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
DFNKVVLANAKTLQFENELSAYDLLAASQTTNLGCYHFIWAENVESAFVGSSPERLYHRK
CCCEEEEECCCEEEECCCCCHHHHHHHCCCCCCCEEEEEECCCCCHHHCCCCHHHHHHHC
GNQFHTEALAGTAPVTESMVQTELNAQWLLTDPKNIYENQLVVDDIQAQLTDCVTELQIS
CCCEEHHHHCCCCHHHHHHHHHHCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHH
QAEIKRLHNVQHLRRKISAVLKPEMSDADCLARMHPTAAVAGLPRQAAKQFIAEYEQFTR
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHH
CWYAGTLGYLQPDEAEFCVALRSAQIERNRITLYAGAGIVEESEPQSEWQEIERKSLALA
HHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
KLLKVD
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]