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The map label for this gene is folE

Identifier: 126208379

GI number: 126208379

Start: 1045805

End: 1046461

Strand: Reverse

Name: folE

Synonym: APL_0903

Alternate gene names: 126208379

Gene position: 1046461-1045805 (Counterclockwise)

Preceding gene: 126208381

Following gene: 126208378

Centisome position: 46.01

GC content: 43.53

Gene sequence:

>657_bases
ATGAGCATTATCTCCGCAGAAGCCCAAAAAGTGCGTGAAGCCTTAATTGCGAAAGGCATTGAAACCCCAACCGTTCAACT
GACTAAAGACAAAGATTCCCGTCGTGCCGAAATTCAACAGCATATGCGTTCGGTACTGGAATTACTGGGATTGGATTTAC
AAGACGATAGTTTGGAAGAAACTCCGCACCGCTTGGCGAAGATGTATGTCGATGAAATTTTTAGCGGCTTGGATTATGCG
ACTTTCCCGAAAATTACCAATATTGAAAATCGTATGAAAGTCAGCGAAATGGTGTTGGTGGACGATATTACCCTGACTTC
CACTTGCGAACACCATTTTGTGACGATTGACGGTAAAGTGGCGGTGGCGTATTACCCGAAAAAATGGGTAATCGGCTTAT
CTAAAATTAATCGCGTGGTGCAATTTTTCGCTCAACGTCCACAGGTGCAAGAGCGTTTTACCGAACAGATCCTAACCGCA
TTCCAAACGATTTTAGAAACGGACGATGTCGCAGTTTTTGTTAAAGCAACACATTTCTGCGTGAAATGCCGCGGCGTGAA
AGATACGAATAGCTATACGGTAACGTCGGCATTCGGCGGCGTTTTCTTAGAAGACAGAGAAACGCGCAAAGAATTTTTGG
GTTTAATCAATAAATAA

Upstream 100 bases:

>100_bases
ACGGATTATTGGGACGAGTTATTATTGAGATAAATTTTAGTGCTATTTTTTTTGATGTCAATTTGTTAAAATTCTCGCCT
TGATTTTTAGAGGAACCCCA

Downstream 100 bases:

>100_bases
TTGAACGGATAAGCGGTCTGATTTTGGCAAATTTTTGCAAAATTTAACCGCTTATCTTTTTTGATGTAGAGCAAAATAAT
GAAAATTGCATTAGGCATTG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSIISAEAQKVREALIAKGIETPTVQLTKDKDSRRAEIQQHMRSVLELLGLDLQDDSLEETPHRLAKMYVDEIFSGLDYA
TFPKITNIENRMKVSEMVLVDDITLTSTCEHHFVTIDGKVAVAYYPKKWVIGLSKINRVVQFFAQRPQVQERFTEQILTA
FQTILETDDVAVFVKATHFCVKCRGVKDTNSYTVTSAFGGVFLEDRETRKEFLGLINK

Sequences:

>Translated_218_residues
MSIISAEAQKVREALIAKGIETPTVQLTKDKDSRRAEIQQHMRSVLELLGLDLQDDSLEETPHRLAKMYVDEIFSGLDYA
TFPKITNIENRMKVSEMVLVDDITLTSTCEHHFVTIDGKVAVAYYPKKWVIGLSKINRVVQFFAQRPQVQERFTEQILTA
FQTILETDDVAVFVKATHFCVKCRGVKDTNSYTVTSAFGGVFLEDRETRKEFLGLINK
>Mature_217_residues
SIISAEAQKVREALIAKGIETPTVQLTKDKDSRRAEIQQHMRSVLELLGLDLQDDSLEETPHRLAKMYVDEIFSGLDYAT
FPKITNIENRMKVSEMVLVDDITLTSTCEHHFVTIDGKVAVAYYPKKWVIGLSKINRVVQFFAQRPQVQERFTEQILTAF
QTILETDDVAVFVKATHFCVKCRGVKDTNSYTVTSAFGGVFLEDRETRKEFLGLINK

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=180, Percent_Identity=44.4444444444444, Blast_Score=134, Evalue=7e-32,
Organism=Homo sapiens, GI4503949, Length=180, Percent_Identity=44.4444444444444, Blast_Score=134, Evalue=7e-32,
Organism=Homo sapiens, GI66932970, Length=141, Percent_Identity=40.4255319148936, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI66932972, Length=141, Percent_Identity=40.4255319148936, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1788476, Length=215, Percent_Identity=64.1860465116279, Blast_Score=279, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17560486, Length=183, Percent_Identity=42.0765027322404, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6321706, Length=193, Percent_Identity=40.4145077720207, Blast_Score=124, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24656796, Length=182, Percent_Identity=40.6593406593407, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24656782, Length=182, Percent_Identity=40.6593406593407, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24656791, Length=122, Percent_Identity=49.1803278688525, Blast_Score=116, Evalue=1e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_ACTP2 (A3N0R3)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053604.1
- ProteinModelPortal:   A3N0R3
- SMR:   A3N0R3
- STRING:   A3N0R3
- GeneID:   4850242
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0903
- eggNOG:   COG0302
- HOGENOM:   HBG370191
- OMA:   ACSHHLC
- PhylomeDB:   A3N0R3
- ProtClustDB:   PRK09347
- BioCyc:   APLE416269:APL_0903-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 24838; Mature: 24707

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2; PS00589 PTS_HPR_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIISAEAQKVREALIAKGIETPTVQLTKDKDSRRAEIQQHMRSVLELLGLDLQDDSLEE
CCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHH
TPHRLAKMYVDEIFSGLDYATFPKITNIENRMKVSEMVLVDDITLTSTCEHHFVTIDGKV
HHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHEEECCEEECCCCCEEEEECCEE
AVAYYPKKWVIGLSKINRVVQFFAQRPQVQERFTEQILTAFQTILETDDVAVFVKATHFC
EEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHEEE
VKCRGVKDTNSYTVTSAFGGVFLEDRETRKEFLGLINK
EEECCCCCCCCEEEEECCCCEEECCHHHHHHHHHHCCC
>Mature Secondary Structure 
SIISAEAQKVREALIAKGIETPTVQLTKDKDSRRAEIQQHMRSVLELLGLDLQDDSLEE
CCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHH
TPHRLAKMYVDEIFSGLDYATFPKITNIENRMKVSEMVLVDDITLTSTCEHHFVTIDGKV
HHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHEEECCEEECCCCCEEEEECCEE
AVAYYPKKWVIGLSKINRVVQFFAQRPQVQERFTEQILTAFQTILETDDVAVFVKATHFC
EEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHEEE
VKCRGVKDTNSYTVTSAFGGVFLEDRETRKEFLGLINK
EEECCCCCCCCEEEEECCCCEEECCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA