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The map label for this gene is truA

Identifier: 126208378

GI number: 126208378

Start: 1044938

End: 1045726

Strand: Reverse

Name: truA

Synonym: APL_0902

Alternate gene names: 126208378

Gene position: 1045726-1044938 (Counterclockwise)

Preceding gene: 126208379

Following gene: 126208376

Centisome position: 45.98

GC content: 46.51

Gene sequence:

>789_bases
ATGAAAATTGCATTAGGCATTGAATATGACGGCAGTCGCTATTTCGGCTGGCAGCGTCAAGATGAAGTGGAAAGCGTGCA
ACAGAAGCTGGAAGAAGCATTGTCGATTGTCGCCAACGCGCCGATAGAGGTTTTTTGTGCCGGACGAACCGATTCCGGCG
TACACGGTACGGGGCAAGTGGTGCATTTTGAAACGCAGGCGATACGTCCGTTACAAAGTTGGTGCTTTGGTACCAATGCG
AATTTACCGGACGATATTGCGGTAAAATGGGCGGTTGAGGTGAGTGAGGATTTTCACGCACGTTTTAGTGCCACGGCAAG
ACGTTATCGTTACATTATTTTTAATAACAAACTGCGTTCGGCAATTTTGCCGAAAGGTGTCTCACATTATCATTATGCTC
TCGATCATCAGAAAATGCACGTAGCTGGTCAGTTTTTGTTAGGCGAAAATGACTTTAGCTCATTCCGTGCCGCTAAATGT
CAATCGCATACCCCTTGGCGTAACGTTCATCATCTGAATGTCAGCCGCTTGGGTAATTATATTGTGGTGGATATTCAAGC
GAATGCTTTTGTGCATCATATGGTGCGCAATATTGTCGAAAGCCTAATTGAAGTCGGGCAGGGCAGACAACCGGTCGAAT
GGATTCAATGGTTATTGGCACAACGAGATCGTACTTTAGCGGCGCCGACCGCAAAGGCGGAAGGTTTATATTTGGTGGAT
GTGCATTATCCGGAGCGTTTCGGTATCCCTAAAACCGCATTAGGTCCGTTATTTCTGGCGGATAATTAA

Upstream 100 bases:

>100_bases
TTTGGGTTTAATCAATAAATAATTGAACGGATAAGCGGTCTGATTTTGGCAAATTTTTGCAAAATTTAACCGCTTATCTT
TTTTGATGTAGAGCAAAATA

Downstream 100 bases:

>100_bases
ATTAAAAAAGCCAAACCGAAATTCGATTTGGCTTTATCTTCAATTGTTATTTAATTTTTTAAACGTTTTTTAAAGTACTG
TCCGTACGACGCGCTTCATT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MKIALGIEYDGSRYFGWQRQDEVESVQQKLEEALSIVANAPIEVFCAGRTDSGVHGTGQVVHFETQAIRPLQSWCFGTNA
NLPDDIAVKWAVEVSEDFHARFSATARRYRYIIFNNKLRSAILPKGVSHYHYALDHQKMHVAGQFLLGENDFSSFRAAKC
QSHTPWRNVHHLNVSRLGNYIVVDIQANAFVHHMVRNIVESLIEVGQGRQPVEWIQWLLAQRDRTLAAPTAKAEGLYLVD
VHYPERFGIPKTALGPLFLADN

Sequences:

>Translated_262_residues
MKIALGIEYDGSRYFGWQRQDEVESVQQKLEEALSIVANAPIEVFCAGRTDSGVHGTGQVVHFETQAIRPLQSWCFGTNA
NLPDDIAVKWAVEVSEDFHARFSATARRYRYIIFNNKLRSAILPKGVSHYHYALDHQKMHVAGQFLLGENDFSSFRAAKC
QSHTPWRNVHHLNVSRLGNYIVVDIQANAFVHHMVRNIVESLIEVGQGRQPVEWIQWLLAQRDRTLAAPTAKAEGLYLVD
VHYPERFGIPKTALGPLFLADN
>Mature_262_residues
MKIALGIEYDGSRYFGWQRQDEVESVQQKLEEALSIVANAPIEVFCAGRTDSGVHGTGQVVHFETQAIRPLQSWCFGTNA
NLPDDIAVKWAVEVSEDFHARFSATARRYRYIIFNNKLRSAILPKGVSHYHYALDHQKMHVAGQFLLGENDFSSFRAAKC
QSHTPWRNVHHLNVSRLGNYIVVDIQANAFVHHMVRNIVESLIEVGQGRQPVEWIQWLLAQRDRTLAAPTAKAEGLYLVD
VHYPERFGIPKTALGPLFLADN

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=285, Percent_Identity=27.719298245614, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1788657, Length=260, Percent_Identity=71.1538461538462, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_ACTP2 (A3N0R2)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053603.1
- ProteinModelPortal:   A3N0R2
- SMR:   A3N0R2
- STRING:   A3N0R2
- GeneID:   4850172
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0902
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   A3N0R2
- ProtClustDB:   PRK00021
- BioCyc:   APLE416269:APL_0902-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29693; Mature: 29693

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIALGIEYDGSRYFGWQRQDEVESVQQKLEEALSIVANAPIEVFCAGRTDSGVHGTGQV
CEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCE
VHFETQAIRPLQSWCFGTNANLPDDIAVKWAVEVSEDFHARFSATARRYRYIIFNNKLRS
EEEEHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECHHHHHHHHHHHHHEEEEEECCCHHH
AILPKGVSHYHYALDHQKMHVAGQFLLGENDFSSFRAAKCQSHTPWRNVHHLNVSRLGNY
HHCCCCCCHHHEECCCCEEEEEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEHHHCCCE
IVVDIQANAFVHHMVRNIVESLIEVGQGRQPVEWIQWLLAQRDRTLAAPTAKAEGLYLVD
EEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEECCCCCCCCEEEEE
VHYPERFGIPKTALGPLFLADN
EECCHHCCCCCHHCCCEEEECC
>Mature Secondary Structure
MKIALGIEYDGSRYFGWQRQDEVESVQQKLEEALSIVANAPIEVFCAGRTDSGVHGTGQV
CEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCE
VHFETQAIRPLQSWCFGTNANLPDDIAVKWAVEVSEDFHARFSATARRYRYIIFNNKLRS
EEEEHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECHHHHHHHHHHHHHEEEEEECCCHHH
AILPKGVSHYHYALDHQKMHVAGQFLLGENDFSSFRAAKCQSHTPWRNVHHLNVSRLGNY
HHCCCCCCHHHEECCCCEEEEEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEHHHCCCE
IVVDIQANAFVHHMVRNIVESLIEVGQGRQPVEWIQWLLAQRDRTLAAPTAKAEGLYLVD
EEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEECCCCCCCCEEEEE
VHYPERFGIPKTALGPLFLADN
EECCHHCCCCCHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA