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The map label for this gene is folD

Identifier: 126208373

GI number: 126208373

Start: 1039775

End: 1040632

Strand: Direct

Name: folD

Synonym: APL_0897

Alternate gene names: 126208373

Gene position: 1039775-1040632 (Clockwise)

Preceding gene: 126208372

Following gene: 126208374

Centisome position: 45.71

GC content: 47.55

Gene sequence:

>858_bases
ATGACGGCACACATTATCTCAGGCACAGCGGTGGCAAAGCAAGTTAAAGCGAATATCGCCGAACAAATTCAAGCCTATAC
CGCACAAGATAAACGTAAACCGGGATTAGCGGTGATTTTAGTGGGAATGGATCCCGCCTCTCAGGTGTATGTGAATAGTA
AACGAAAAAGTTGTGCCGAAATCGGTATCGAATCCAAATCGTACGATCTGCCTGCCGAAACCGGTGAAGCCGAATTACTT
GCGATTATCGAACAATTAAACCATGACGATTCGGTCGATGGCATTCTGGTGCAATTACCGCTACCGAAGCAAATTGATGC
GACTAAAGTTACCGAAGCGATTGTGCCGCATAAAGATGTGGACGGTTTCCATCCTTACAATGTCGGACGTTTATGCCAAA
AAATTCCGACCTTACGCTCTTGTACACCATACGGCGTTATGAAATTACTGGAAAGTACCGGCGTGAATCTCGCCGGATTA
CATGCCGTTGTTGTCGGTGCTTCAAATATTGTCGGTCGTCCGATGGCAATGGAACTACTGCTTGCCGGTTGTACCGTTAC
CATCACACACAGCCGCACTAAAGATTTGGCTTATCACGTTTCGCAAGCGGATATTGTGGTTGCCGGAGTCGGCAAACCGA
ACTTTGTAAAAGGTGAATGGATTAAACCGGGTGCGATTGTGATTGATGTCGGCATTAACCGAGTGGAAGGCAAATTAATC
GGTGACGTGGAATACTCCGCTGCGGAAGCTAAAGCGAGCTTCATCACGCCGGTACCGGGCGGTGTCGGTCCGATGACGGT
AGCGATGTTAATGCAAAATACCTTGCAAGCCTATCAAGTTCATTTACAAGCGGTCTGA

Upstream 100 bases:

>100_bases
GTATTTATACATGGCGATCTTTAATTTACCTACTTGCAAATCAACCGTTTTCTGACAAAATAGCAAACGTTTGCAATCTA
ATCGAATCAAGGGAGAAACA

Downstream 100 bases:

>100_bases
TTTTCAATTTATTCCGCAATTAAGCCTCTTTTGAAAAAAAGAGGCTTTTTTACTGAATATTCTCTTACGCAAACGTTTGC
GCAAATGATTTTTGCTGTTA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTAHIISGTAVAKQVKANIAEQIQAYTAQDKRKPGLAVILVGMDPASQVYVNSKRKSCAEIGIESKSYDLPAETGEAELL
AIIEQLNHDDSVDGILVQLPLPKQIDATKVTEAIVPHKDVDGFHPYNVGRLCQKIPTLRSCTPYGVMKLLESTGVNLAGL
HAVVVGASNIVGRPMAMELLLAGCTVTITHSRTKDLAYHVSQADIVVAGVGKPNFVKGEWIKPGAIVIDVGINRVEGKLI
GDVEYSAAEAKASFITPVPGGVGPMTVAMLMQNTLQAYQVHLQAV

Sequences:

>Translated_285_residues
MTAHIISGTAVAKQVKANIAEQIQAYTAQDKRKPGLAVILVGMDPASQVYVNSKRKSCAEIGIESKSYDLPAETGEAELL
AIIEQLNHDDSVDGILVQLPLPKQIDATKVTEAIVPHKDVDGFHPYNVGRLCQKIPTLRSCTPYGVMKLLESTGVNLAGL
HAVVVGASNIVGRPMAMELLLAGCTVTITHSRTKDLAYHVSQADIVVAGVGKPNFVKGEWIKPGAIVIDVGINRVEGKLI
GDVEYSAAEAKASFITPVPGGVGPMTVAMLMQNTLQAYQVHLQAV
>Mature_284_residues
TAHIISGTAVAKQVKANIAEQIQAYTAQDKRKPGLAVILVGMDPASQVYVNSKRKSCAEIGIESKSYDLPAETGEAELLA
IIEQLNHDDSVDGILVQLPLPKQIDATKVTEAIVPHKDVDGFHPYNVGRLCQKIPTLRSCTPYGVMKLLESTGVNLAGLH
AVVVGASNIVGRPMAMELLLAGCTVTITHSRTKDLAYHVSQADIVVAGVGKPNFVKGEWIKPGAIVIDVGINRVEGKLIG
DVEYSAAEAKASFITPVPGGVGPMTVAMLMQNTLQAYQVHLQAV

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=297, Percent_Identity=46.1279461279461, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI94721354, Length=291, Percent_Identity=46.0481099656357, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI222418558, Length=291, Percent_Identity=43.6426116838488, Blast_Score=228, Evalue=6e-60,
Organism=Homo sapiens, GI36796743, Length=230, Percent_Identity=30, Blast_Score=88, Evalue=9e-18,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=63.3093525179856, Blast_Score=371, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17568735, Length=288, Percent_Identity=43.4027777777778, Blast_Score=205, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6319558, Length=284, Percent_Identity=49.2957746478873, Blast_Score=258, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6321643, Length=301, Percent_Identity=44.1860465116279, Blast_Score=240, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322933, Length=316, Percent_Identity=27.2151898734177, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17136816, Length=293, Percent_Identity=46.0750853242321, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI17136818, Length=293, Percent_Identity=46.0750853242321, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI62472483, Length=292, Percent_Identity=46.2328767123288, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI45551871, Length=292, Percent_Identity=46.2328767123288, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24645718, Length=292, Percent_Identity=46.2328767123288, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI17137370, Length=292, Percent_Identity=46.2328767123288, Blast_Score=236, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_ACTP2 (A3N0Q7)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053598.1
- ProteinModelPortal:   A3N0Q7
- SMR:   A3N0Q7
- STRING:   A3N0Q7
- GeneID:   4850107
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0897
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- PhylomeDB:   A3N0Q7
- ProtClustDB:   PRK10792
- BioCyc:   APLE416269:APL_0897-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30266; Mature: 30134

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAHIISGTAVAKQVKANIAEQIQAYTAQDKRKPGLAVILVGMDPASQVYVNSKRKSCAE
CCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCHHHHHHH
IGIESKSYDLPAETGEAELLAIIEQLNHDDSVDGILVQLPLPKQIDATKVTEAIVPHKDV
HCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCC
DGFHPYNVGRLCQKIPTLRSCTPYGVMKLLESTGVNLAGLHAVVVGASNIVGRPMAMELL
CCCCCCCHHHHHHHCCCHHCCCHHHHHHHHHHCCCCHHHHEEEEEECHHHCCCHHHHHHH
LAGCTVTITHSRTKDLAYHVSQADIVVAGVGKPNFVKGEWIKPGAIVIDVGINRVEGKLI
HHCCEEEEECCCCCHHHEEECCCCEEEEECCCCCCCCCCEECCCEEEEEECCCEECCEEE
GDVEYSAAEAKASFITPVPGGVGPMTVAMLMQNTLQAYQVHLQAV
ECCCCCHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
TAHIISGTAVAKQVKANIAEQIQAYTAQDKRKPGLAVILVGMDPASQVYVNSKRKSCAE
CEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCHHHHHHH
IGIESKSYDLPAETGEAELLAIIEQLNHDDSVDGILVQLPLPKQIDATKVTEAIVPHKDV
HCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCC
DGFHPYNVGRLCQKIPTLRSCTPYGVMKLLESTGVNLAGLHAVVVGASNIVGRPMAMELL
CCCCCCCHHHHHHHCCCHHCCCHHHHHHHHHHCCCCHHHHEEEEEECHHHCCCHHHHHHH
LAGCTVTITHSRTKDLAYHVSQADIVVAGVGKPNFVKGEWIKPGAIVIDVGINRVEGKLI
HHCCEEEEECCCCCHHHEEECCCCEEEEECCCCCCCCCCEECCCEEEEEECCCEECCEEE
GDVEYSAAEAKASFITPVPGGVGPMTVAMLMQNTLQAYQVHLQAV
ECCCCCHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA